Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue

Detalhes bibliográficos
Autor(a) principal: Zambuzzi, Willian Fernando
Data de Publicação: 2005
Outros Autores: Oliveira, Rodrigo Cardoso de, Alanis, Danilo, Menezes, Renato, Letra, Ariadne, Cestari, Tânia Mary, Taga, Rumio, Granjeiro, José Mauro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3312
Resumo: The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue.
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spelling Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue Análise microscópica do osso bovino inorgânico microgranular implantado em subcutâneo de ratos Osso medularAnálise histológicaBiocompatibilidadeEnxerto ósseoXenoenxertoBone marrowHistological analysisBiocompatibilityBone graftXenograft The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue. A resposta tecidual ao osso inorgânico bovino medular implantado em subcutâneo de rato foi avaliada por análise subjetiva através de microscopia de luz. Quarenta ratos foram divididos em 2 grupos: controle (cápsulas vazias de colágeno) e teste (cápsulas de colágeno contendo 0,1g do biomaterial) e mortos 10, 20, 30 e 60 dias após a implantação. Histologicamente, aos 10 dias, observou-se infiltrado inflamatório crônico composto por macrófagos e Células Gigantes Multinucleadas Inflamatórias (IMGC). Neutrófilos, plasmócitos e linfócitos estavam presentes de maneira discreta, desaparecendo durante o processo de reparo tecidual. A porosidade do material foi preenchida pelo tecido conjuntivo reacional mostrando as IMGC. A fibrose foi mais intensa aos 60 dias e evidentemente superior ao grupo controle. Entretanto, o material não causou reações adversas severas, não estimulando a resposta imunológica. Baseado nos resultados encontrados, concluímos que o osso inorgânico bovino medular foi bem tolerado pelo tecido conjuntivo de rato. Universidade de São Paulo. Faculdade de Odontologia de Bauru2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/331210.1590/S1678-77572005000400013Journal of Applied Oral Science; Vol. 13 No. 4 (2005); 382-386 Journal of Applied Oral Science; Vol. 13 Núm. 4 (2005); 382-386 Journal of Applied Oral Science; v. 13 n. 4 (2005); 382-386 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3312/4002Copyright (c) 2005 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessZambuzzi, Willian FernandoOliveira, Rodrigo Cardoso deAlanis, DaniloMenezes, RenatoLetra, AriadneCestari, Tânia MaryTaga, RumioGranjeiro, José Mauro2012-04-27T11:51:56Zoai:revistas.usp.br:article/3312Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:51:56Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
Análise microscópica do osso bovino inorgânico microgranular implantado em subcutâneo de ratos
title Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
spellingShingle Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
Zambuzzi, Willian Fernando
Osso medular
Análise histológica
Biocompatibilidade
Enxerto ósseo
Xenoenxerto
Bone marrow
Histological analysis
Biocompatibility
Bone graft
Xenograft
title_short Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
title_full Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
title_fullStr Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
title_full_unstemmed Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
title_sort Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue
author Zambuzzi, Willian Fernando
author_facet Zambuzzi, Willian Fernando
Oliveira, Rodrigo Cardoso de
Alanis, Danilo
Menezes, Renato
Letra, Ariadne
Cestari, Tânia Mary
Taga, Rumio
Granjeiro, José Mauro
author_role author
author2 Oliveira, Rodrigo Cardoso de
Alanis, Danilo
Menezes, Renato
Letra, Ariadne
Cestari, Tânia Mary
Taga, Rumio
Granjeiro, José Mauro
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zambuzzi, Willian Fernando
Oliveira, Rodrigo Cardoso de
Alanis, Danilo
Menezes, Renato
Letra, Ariadne
Cestari, Tânia Mary
Taga, Rumio
Granjeiro, José Mauro
dc.subject.por.fl_str_mv Osso medular
Análise histológica
Biocompatibilidade
Enxerto ósseo
Xenoenxerto
Bone marrow
Histological analysis
Biocompatibility
Bone graft
Xenograft
topic Osso medular
Análise histológica
Biocompatibilidade
Enxerto ósseo
Xenoenxerto
Bone marrow
Histological analysis
Biocompatibility
Bone graft
Xenograft
description The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3312
10.1590/S1678-77572005000400013
url https://www.revistas.usp.br/jaos/article/view/3312
identifier_str_mv 10.1590/S1678-77572005000400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3312/4002
dc.rights.driver.fl_str_mv Copyright (c) 2005 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2005 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 13 No. 4 (2005); 382-386
Journal of Applied Oral Science; Vol. 13 Núm. 4 (2005); 382-386
Journal of Applied Oral Science; v. 13 n. 4 (2005); 382-386
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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