Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?

Detalhes bibliográficos
Autor(a) principal: Silva, Paulo Marcos Amaral
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/7853
Resumo: The dioecy occurs in 6% of the angiosperms, including all the Myrsine species (Primulaceae) that show male and female individuals distinguishable based in sexual organs and morphology of sepals and petals. The sexual system fixed in this genus, was the motivation in research if the existence of sexual chromosomes culminate in dioecy for Myrsine. Employing tissue culture, flow cytometry and cytogenetic tools, the aimed this study was characterize the karyotype of Myrsine coriacea (Sw.) R.Br. ex Roem & Schult., Myrsine umbellata Mart., Myrsine guianensis (Aubl.) Kuntze and Myrsine parvifolia A.DC. From leaves of male and female individuals collected in the field and leaves and roots of unknown sex obtained of in vitro culture, were found mean values of DNA content statistically identical. These data were attributed to the presence of secondary metabolites reported for the genus. However, an intraspecific variation was observed in cytogenetic analysis in the four Myrsine species. Slides exhibited metaphases with 2n = 45 and 2n = 46 chromosomes in different individuals. These results were observed consistently from different times of exposition to anti-mitotic agent and distinct treatments of enzymatic maceration. Thus, the chromosome number variation found, associated with the sexual system well defined, can be concerning to the sexual chromosomes in Myrsine genus. These data suggest a chromosomal system of sex 9 determination with multiple X and/or Y described in some plant species. The system ZW also is possible, as well as X0 or Z0, systems still not reported in vegetal groups. The present work provided the first data about the nuclear DNA content by flow cytometry in Myrsine and supplied cytogenetics evidences that indicate the existence of sexual chromosomes.
id UFES_53ed4ec12454c95706d24ee1fa072a58
oai_identifier_str oai:repositorio.ufes.br:10/7853
network_acronym_str UFES
network_name_str Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
repository_id_str 2108
spelling Carrijo, Tatiana TavaresFontes, Milene Miranda PraçaSilva, Paulo Marcos AmaralClarindo, Wellington RonildoGonçalves, Rosado, Carla Cristina2018-08-01T22:57:30Z2018-08-012018-08-01T22:57:30Z2015-08-14The dioecy occurs in 6% of the angiosperms, including all the Myrsine species (Primulaceae) that show male and female individuals distinguishable based in sexual organs and morphology of sepals and petals. The sexual system fixed in this genus, was the motivation in research if the existence of sexual chromosomes culminate in dioecy for Myrsine. Employing tissue culture, flow cytometry and cytogenetic tools, the aimed this study was characterize the karyotype of Myrsine coriacea (Sw.) R.Br. ex Roem & Schult., Myrsine umbellata Mart., Myrsine guianensis (Aubl.) Kuntze and Myrsine parvifolia A.DC. From leaves of male and female individuals collected in the field and leaves and roots of unknown sex obtained of in vitro culture, were found mean values of DNA content statistically identical. These data were attributed to the presence of secondary metabolites reported for the genus. However, an intraspecific variation was observed in cytogenetic analysis in the four Myrsine species. Slides exhibited metaphases with 2n = 45 and 2n = 46 chromosomes in different individuals. These results were observed consistently from different times of exposition to anti-mitotic agent and distinct treatments of enzymatic maceration. Thus, the chromosome number variation found, associated with the sexual system well defined, can be concerning to the sexual chromosomes in Myrsine genus. These data suggest a chromosomal system of sex 9 determination with multiple X and/or Y described in some plant species. The system ZW also is possible, as well as X0 or Z0, systems still not reported in vegetal groups. The present work provided the first data about the nuclear DNA content by flow cytometry in Myrsine and supplied cytogenetics evidences that indicate the existence of sexual chromosomes.Texthttp://repositorio.ufes.br/handle/10/7853porUniversidade Federal do Espírito SantoMestrado em Genética e MelhoramentoPrograma de Pós-Graduação em Genética e MelhoramentoUFESBRCytogeneticFlow cytometrySex chromosomesSex determinatCitogenéticaCitometria de fluxoCromossomos sexuaisSexo - Causa e determinaçãoMelhoramento Vegetal631.523Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_9189_Dissertação Final Paulo Marcos Amaral Silva20160628-103338.pdfapplication/pdf832244http://repositorio.ufes.br/bitstreams/4c651b5a-ad45-43ff-a206-933180559872/downloadde4cc3d0c60078e1f76f3d4f95f3b534MD5110/78532024-06-24 10:17:35.548oai:repositorio.ufes.br:10/7853http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-07-11T14:29:51.508616Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
title Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
spellingShingle Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
Silva, Paulo Marcos Amaral
Cytogenetic
Flow cytometry
Sex chromosomes
Sex determinat
Melhoramento Vegetal
Citogenética
Citometria de fluxo
Cromossomos sexuais
Sexo - Causa e determinação
631.523
title_short Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
title_full Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
title_fullStr Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
title_full_unstemmed Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
title_sort Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
author Silva, Paulo Marcos Amaral
author_facet Silva, Paulo Marcos Amaral
author_role author
dc.contributor.advisor-co1.fl_str_mv Carrijo, Tatiana Tavares
dc.contributor.advisor1.fl_str_mv Fontes, Milene Miranda Praça
dc.contributor.author.fl_str_mv Silva, Paulo Marcos Amaral
dc.contributor.referee1.fl_str_mv Clarindo, Wellington Ronildo
dc.contributor.referee2.fl_str_mv Gonçalves, Rosado, Carla Cristina
contributor_str_mv Carrijo, Tatiana Tavares
Fontes, Milene Miranda Praça
Clarindo, Wellington Ronildo
Gonçalves, Rosado, Carla Cristina
dc.subject.por.fl_str_mv Cytogenetic
Flow cytometry
Sex chromosomes
Sex determinat
topic Cytogenetic
Flow cytometry
Sex chromosomes
Sex determinat
Melhoramento Vegetal
Citogenética
Citometria de fluxo
Cromossomos sexuais
Sexo - Causa e determinação
631.523
dc.subject.cnpq.fl_str_mv Melhoramento Vegetal
dc.subject.br-rjbn.none.fl_str_mv Citogenética
Citometria de fluxo
Cromossomos sexuais
Sexo - Causa e determinação
dc.subject.udc.none.fl_str_mv 631.523
description The dioecy occurs in 6% of the angiosperms, including all the Myrsine species (Primulaceae) that show male and female individuals distinguishable based in sexual organs and morphology of sepals and petals. The sexual system fixed in this genus, was the motivation in research if the existence of sexual chromosomes culminate in dioecy for Myrsine. Employing tissue culture, flow cytometry and cytogenetic tools, the aimed this study was characterize the karyotype of Myrsine coriacea (Sw.) R.Br. ex Roem & Schult., Myrsine umbellata Mart., Myrsine guianensis (Aubl.) Kuntze and Myrsine parvifolia A.DC. From leaves of male and female individuals collected in the field and leaves and roots of unknown sex obtained of in vitro culture, were found mean values of DNA content statistically identical. These data were attributed to the presence of secondary metabolites reported for the genus. However, an intraspecific variation was observed in cytogenetic analysis in the four Myrsine species. Slides exhibited metaphases with 2n = 45 and 2n = 46 chromosomes in different individuals. These results were observed consistently from different times of exposition to anti-mitotic agent and distinct treatments of enzymatic maceration. Thus, the chromosome number variation found, associated with the sexual system well defined, can be concerning to the sexual chromosomes in Myrsine genus. These data suggest a chromosomal system of sex 9 determination with multiple X and/or Y described in some plant species. The system ZW also is possible, as well as X0 or Z0, systems still not reported in vegetal groups. The present work provided the first data about the nuclear DNA content by flow cytometry in Myrsine and supplied cytogenetics evidences that indicate the existence of sexual chromosomes.
publishDate 2015
dc.date.issued.fl_str_mv 2015-08-14
dc.date.accessioned.fl_str_mv 2018-08-01T22:57:30Z
dc.date.available.fl_str_mv 2018-08-01
2018-08-01T22:57:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufes.br/handle/10/7853
url http://repositorio.ufes.br/handle/10/7853
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv Text
dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Genética e Melhoramento
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Genética e Melhoramento
dc.publisher.initials.fl_str_mv UFES
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Genética e Melhoramento
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
instname:Universidade Federal do Espírito Santo (UFES)
instacron:UFES
instname_str Universidade Federal do Espírito Santo (UFES)
instacron_str UFES
institution UFES
reponame_str Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
collection Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
bitstream.url.fl_str_mv http://repositorio.ufes.br/bitstreams/4c651b5a-ad45-43ff-a206-933180559872/download
bitstream.checksum.fl_str_mv de4cc3d0c60078e1f76f3d4f95f3b534
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)
repository.mail.fl_str_mv
_version_ 1813022599945388032