Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Alessandra Casagrande
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/1363
Resumo: Pink pepper is the fruit of the pepper tree (Schinus terebinthifolius Raddi), a native plant from Brazil. The extraction and characterization of its essential oil enables its industrial use. This work aimed to evaluate the effect of essential oil of pink pepper addition on Minas Frescal cheese. 95.36% of constituents of essential oil were identified, and the d-carene-3 was the major compontente. Pink pepper essential oil showed antioxidant (2.526 ± 0.277 µmols per ml Trolox oil) and antimicrobial characteristics regarding the development of Staphylococcus aureus. It was observed in the agar diffusion test, the formation of inhibition zone of 1.35 ± 0.322 cm, and in the minimum inhibitory concentration analysis, the value of 3.13%. Essential oil is sensitive to degradation, so it requires technological processes to ensure its action. In order to protect the essential oil, it was microencapsulated with the use of a spray dryer. To microencapsulate it, three wallpaper formulations were developed using different concentrations of gum arabic, modified starch and maltodextrin. The microcapsules were analyzed according to their physical, chemical and morphological characteristics and a formulation was selected to be added to Minas Frescal cheese. The formulation with 5% modified starch, 10% of maltodextrin and 5% arabic gum was selected. In order to define the cheese essential oil concentration, it was conducted a sensory test-ordering preference and the formulation with 0.01% essential oil was the most preferred. Control cheese (CO) and cheese with addition of pink pepper essential oil microcapsules (OEPR) were prepared and characterized. Concerning physico-chemical characteristics, CO and OEPR cheeses only differed on moisture. OEPR cheese presented good sensory acceptance. Global acceptance means were 7.6 ± 0.97, with no significant difference (p> 0.05) compared to control cheese (7.6 ± 1.18). To evaluate the effect of pink pepper essential oil on inhibiting the development of S. aureus, the oil was inoculated in CO and OEPR cheeses with initial concentrations of 106 CFU.g-1. The cheeses were stored in BOD at temperature of 4 ± 1 °C during the period of time of analysis: 0, 3, 6, 9, 12, 15 and 30 days. Results showed reduction of 1.53 cycles Log in cheese with the addition of pink pepper essential oil. According to these results, pink pepper essential oil is a feasible alternative of addition in Minas Frescal cheese due to its antioxidant and antimicrobial potential, and its sensory acceptance.
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spelling Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescalEffect of microencapsulated essential oil of pink pepper (Schinus terebinthifolius Raddi) addition on Minas Frescal cheeseMicroencapsulationSpray dryingMicroencapsulaçãoSecagem por atomizaçãoStaphylococcus aureusCiência e Tecnologia de Alimentos664Pink pepper is the fruit of the pepper tree (Schinus terebinthifolius Raddi), a native plant from Brazil. The extraction and characterization of its essential oil enables its industrial use. This work aimed to evaluate the effect of essential oil of pink pepper addition on Minas Frescal cheese. 95.36% of constituents of essential oil were identified, and the d-carene-3 was the major compontente. Pink pepper essential oil showed antioxidant (2.526 ± 0.277 µmols per ml Trolox oil) and antimicrobial characteristics regarding the development of Staphylococcus aureus. It was observed in the agar diffusion test, the formation of inhibition zone of 1.35 ± 0.322 cm, and in the minimum inhibitory concentration analysis, the value of 3.13%. Essential oil is sensitive to degradation, so it requires technological processes to ensure its action. In order to protect the essential oil, it was microencapsulated with the use of a spray dryer. To microencapsulate it, three wallpaper formulations were developed using different concentrations of gum arabic, modified starch and maltodextrin. The microcapsules were analyzed according to their physical, chemical and morphological characteristics and a formulation was selected to be added to Minas Frescal cheese. The formulation with 5% modified starch, 10% of maltodextrin and 5% arabic gum was selected. In order to define the cheese essential oil concentration, it was conducted a sensory test-ordering preference and the formulation with 0.01% essential oil was the most preferred. Control cheese (CO) and cheese with addition of pink pepper essential oil microcapsules (OEPR) were prepared and characterized. Concerning physico-chemical characteristics, CO and OEPR cheeses only differed on moisture. OEPR cheese presented good sensory acceptance. Global acceptance means were 7.6 ± 0.97, with no significant difference (p> 0.05) compared to control cheese (7.6 ± 1.18). To evaluate the effect of pink pepper essential oil on inhibiting the development of S. aureus, the oil was inoculated in CO and OEPR cheeses with initial concentrations of 106 CFU.g-1. The cheeses were stored in BOD at temperature of 4 ± 1 °C during the period of time of analysis: 0, 3, 6, 9, 12, 15 and 30 days. Results showed reduction of 1.53 cycles Log in cheese with the addition of pink pepper essential oil. According to these results, pink pepper essential oil is a feasible alternative of addition in Minas Frescal cheese due to its antioxidant and antimicrobial potential, and its sensory acceptance.A pimenta rosa é o fruto da aroeira (Schinus terebinthifolius Raddi), uma planta nativa do Brasil. A extração e caracterização do seu óleo essencial viabiliza a sua utilização industrial. Este trabalho teve como objetivo avaliar o efeito da adição de óleo essencial de pimenta rosa em queijo Minas Frescal . Foram identificados 95,36% dos constituintes do óleo essencial, sendo o δ-careno-3 o componente majoritário. O óleo essencial de pimenta rosa apresentou características antioxidante (2,53 ± 0,28 μmols de trolox por mL de óleo) e antimicrobiana para o desenvolvimento de Staphylococcus aureus. Foi observado, no teste de difusão em ágar, a formação do halo de inibição de 1,35 ± 0,32 cm, e na análise de concentração inibitória mínima, o valor de 3,13%. O óleo essencial é sensível à degradação no meio em que se encontra, portanto necessita de processos tecnológicos para assegurar sua ação. Para proteger o óleo essencial foi realizada a microencapsulação por meio da secagem por spray dryer. O óleo foi microencapsulado utilizando três formulações com diferentes concentrações de goma arábica, maltodextrina e amido modificado como papel de parede. As microcápsulas obtidas foram analisadas quanto às suas características físico-químicas e morfológicas e foi selecionada uma formulação para posterior adição ao queijo Minas Frescal. A formulação com 5% de amido modificado, 10% de maltodextrina e 5% de goma arábica foi selecionada. Para definição da concentração de óleo essencial que foi utilzada no queijo, foi realizado teste sensorial de ordenação-preferência, visto que a concentração inibitória mínima (3,13%) era muito alta para ser utilizada nos queijos. A formulação com 0,01% de óleo essencial foi a preferida e utilizada para fabricação dos queijos. Foram elaborados e caracterizados os queijos controle (CO) e o queijo com adição das microcápsulas de óleo essencial de pimenta rosa (OEPR). Em relação às características físico-químicas, os queijos CO e OEPR só apresentaram diferença significativa em relação à umidade (P= 0,0021; α=0,05). O queijo OEPR teve boa aceitação sensorial com média de aceitação global de 7,6 ± 0,97, não apresentando diferença significativa, ao nível de 95% de confiança, do queijo controle (7,6 ± 1,18). Para avaliar a capacidade do óleo essencial de pimenta rosa microencapsulado em inibir o desenvolvimento de S. aureus, foram inoculadas nos queijos CO e OEPR concentrações iniciais de 106 UFG.g. Os queijos foram armazenados em BOD à temperatura de 4 ± 1 °C até realização das análises, que ocorreram nos tempos: 0, 3, 6, 9, 12, 15 e 30 dias. Houve redução de 1,53 ciclos Log no queijo adicionado de óleo essencial de pimenta rosa. De acordo com os resultados obtidos, conclui-se que o óleo essencial de pimenta rosa é uma alternativa viável de utilização em queijo Minas Frescal , devido ao seu potencial antioxidante, antimicrobiano e à sua aceitação sensorial.Universidade Federal do Espírito SantoBRMestrado em Ciência e Tecnologia de AlimentosCentro de Ciências Agrárias e EngenhariasUFESPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosBernardes, Patrícia CamposSilva, Pollyanna IbrahimBosi, Mirela GuedesPinheiro, Patrícia FontesSaraiva, Sérgio HenriquesRibeiro, Alessandra Casagrande2015-04-30T20:10:18Z2016-06-24T06:00:07Z2015-02-122015-02-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTextapplication/pdfhttp://repositorio.ufes.br/handle/10/1363porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFES2024-06-21T16:56:13Zoai:repositorio.ufes.br:10/1363Repositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-06-21T16:56:13Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
Effect of microencapsulated essential oil of pink pepper (Schinus terebinthifolius Raddi) addition on Minas Frescal cheese
title Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
spellingShingle Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
Ribeiro, Alessandra Casagrande
Microencapsulation
Spray drying
Microencapsulação
Secagem por atomização
Staphylococcus aureus
Ciência e Tecnologia de Alimentos
664
title_short Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
title_full Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
title_fullStr Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
title_full_unstemmed Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
title_sort Efeito da adição de óleo essencial de pimenta rosa (Schinus terebinthifolius Raddi) microencapsulado em queijo minas frescal
author Ribeiro, Alessandra Casagrande
author_facet Ribeiro, Alessandra Casagrande
author_role author
dc.contributor.none.fl_str_mv Bernardes, Patrícia Campos
Silva, Pollyanna Ibrahim
Bosi, Mirela Guedes
Pinheiro, Patrícia Fontes
Saraiva, Sérgio Henriques
dc.contributor.author.fl_str_mv Ribeiro, Alessandra Casagrande
dc.subject.por.fl_str_mv Microencapsulation
Spray drying
Microencapsulação
Secagem por atomização
Staphylococcus aureus
Ciência e Tecnologia de Alimentos
664
topic Microencapsulation
Spray drying
Microencapsulação
Secagem por atomização
Staphylococcus aureus
Ciência e Tecnologia de Alimentos
664
description Pink pepper is the fruit of the pepper tree (Schinus terebinthifolius Raddi), a native plant from Brazil. The extraction and characterization of its essential oil enables its industrial use. This work aimed to evaluate the effect of essential oil of pink pepper addition on Minas Frescal cheese. 95.36% of constituents of essential oil were identified, and the d-carene-3 was the major compontente. Pink pepper essential oil showed antioxidant (2.526 ± 0.277 µmols per ml Trolox oil) and antimicrobial characteristics regarding the development of Staphylococcus aureus. It was observed in the agar diffusion test, the formation of inhibition zone of 1.35 ± 0.322 cm, and in the minimum inhibitory concentration analysis, the value of 3.13%. Essential oil is sensitive to degradation, so it requires technological processes to ensure its action. In order to protect the essential oil, it was microencapsulated with the use of a spray dryer. To microencapsulate it, three wallpaper formulations were developed using different concentrations of gum arabic, modified starch and maltodextrin. The microcapsules were analyzed according to their physical, chemical and morphological characteristics and a formulation was selected to be added to Minas Frescal cheese. The formulation with 5% modified starch, 10% of maltodextrin and 5% arabic gum was selected. In order to define the cheese essential oil concentration, it was conducted a sensory test-ordering preference and the formulation with 0.01% essential oil was the most preferred. Control cheese (CO) and cheese with addition of pink pepper essential oil microcapsules (OEPR) were prepared and characterized. Concerning physico-chemical characteristics, CO and OEPR cheeses only differed on moisture. OEPR cheese presented good sensory acceptance. Global acceptance means were 7.6 ± 0.97, with no significant difference (p> 0.05) compared to control cheese (7.6 ± 1.18). To evaluate the effect of pink pepper essential oil on inhibiting the development of S. aureus, the oil was inoculated in CO and OEPR cheeses with initial concentrations of 106 CFU.g-1. The cheeses were stored in BOD at temperature of 4 ± 1 °C during the period of time of analysis: 0, 3, 6, 9, 12, 15 and 30 days. Results showed reduction of 1.53 cycles Log in cheese with the addition of pink pepper essential oil. According to these results, pink pepper essential oil is a feasible alternative of addition in Minas Frescal cheese due to its antioxidant and antimicrobial potential, and its sensory acceptance.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-30T20:10:18Z
2015-02-12
2015-02-12
2016-06-24T06:00:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufes.br/handle/10/1363
url http://repositorio.ufes.br/handle/10/1363
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv Text
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
BR
Mestrado em Ciência e Tecnologia de Alimentos
Centro de Ciências Agrárias e Engenharias
UFES
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
BR
Mestrado em Ciência e Tecnologia de Alimentos
Centro de Ciências Agrárias e Engenharias
UFES
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
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instname:Universidade Federal do Espírito Santo (UFES)
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institution UFES
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)
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