Análise epidemiológica, histopatológica e imuno-histoquímica de ameloblastomas : casuística de seis anos

• Autor(a) principal: Rocha, Regina Furbino Villefort
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
• Texto Completo: http://repositorio.ufes.br/handle/10/5866
• topic: Continuity of patient care; Lost to follow up; Histology; World Health Organization; Beta-catenin; Immunohistochemical; Ameloblastoma; Continuidade de assistência ao paciente; Perda de seguimento; Histologia; Organização Mundial da Saúde; Beta catenina; Imuno-histoquímica; Odontologia; Acompanhamento terapêutico; Diagnóstico molecular; Histopatologia; Imunohistoquímica; Tumores odontogênicos; Transdução de sinal celular; 616.314

Ameloblastomas are odontogenic tumors (OTs) derived from epithelium which etiology remains unknown. However, recent studies have identified molecular changes associated with the development and progression of OTs, including cell adhesion molecules like E-cadherin and beta-catenin. Objectives: to conduct an epidemiological investigation of ameloblastomas cases from files of the Anatomical Pathology Service at Federal University of Espírito Santo (SAPB-UFES), analyze their histopathological features and the expression of beta-catenin in different variants of ameloblastomas. Methods: a retrospective study of ameloblastomas registered at SAPB-UFES between March 2004 and December 2010. Sociodemographic, clinical and imaginological data were collected, as well as data about access, diagnosis, treatment and follow up of these patients. The histopathological analyzes were based on Vickers and Gorlin, Waldron and El-Mofty and the World Health Organization criteria. Primary antibody anti beta-catenin mouse monoclonal and indirect immuno-peroxidase technique was employed for immunohistochemical analysis. Intensity and location of the immunostaining were analysed. For semiquantitative analysis the scores were: negative, focal, variable and uniformity positivity. Results: there were 13 ameloblastomas, histopathologically classified as solid (06), unicystic (03) and desmoplastic (03). All of them were immunostained. The intensity of immunostaining ranged from weak to strong (1-3). The mean of immunostaining ranged from 10.82% to 13.38% in the nucleus; from 39.93% to 47.61% in the membrane; and from 90.01% to 98.53% in the cytoplasm. However, there was no significant difference in expression of beta-catenin between three different types of ameloblastomas. Conclusion: The results were similar to other epidemiological studies. The cytoplasmic expression of beta-catenin shows accumulation in the cytoplasm and suggests changes in the Wnt signaling pathway. Moreover, the reduction of membrane expression suggests changes in cell adhesion.