Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica

Detalhes bibliográficos
Autor(a) principal: Silva, Laíz Aparecida Azevedo
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/8105
Resumo: Cardiovascular diseases (CVD) are the major responsible factor for morbidity and mortality rates worldwide. The intake of foods rich in antioxidants, such as acai, can help reducing the risk of CVD. The objective of this study was to investigate the effect of the jussara acai (Euterpe edulis Martius) on cardiovascular risk biomarcador in mice submitted to a high fat diet. The pulp of jussara acai was lyophilized and analysed for its chemical composition, phenolic compounds, anthocyanins and antioxidant capacity. For the biological assay, 43 adult male Swiss mice were used. Initially, the animals were submitted a control diet (AIN-93M) (n = 22) or high fat diet (n = 21) for 8 weeks. For a period of additional 8 weeks, the group received the control diet (AIN-93M): control diet (AIN-93M) (CT, n = 10) or control diet (AIN-93M) supplemented with 2% lyophilized jussara açai pulp (CT+A, n = 12); the group received high fat diet: high fat diet (HF, n = 9) or high fat diet supplemented with 2% lyophilized jussara acai pulp (HF+A, n = 12). At the end of the experiment the animals were euthanized and blood samples collected for analysis: aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids, glucose, activator inhibitor plasminogen type 1 (PAI-1), intercellular cell adhesion molecule (ICAM), e-selectin, were histologically analyzed liver and adipose tissue. The results were analyzed by t test to evaluate the control and HF groups prior to supplementation. The 'two-way' analysis of variance (ANOVA) was performed to evaluate the effect of diet and fruit and/or its interaction among the experimental groups (CT, HF, CT+A and HF+A). The data were presented as mean and standard error, with 5% significance level. The experimental groups CT, CT+A and HF+A had less than 33% of hepatocytes with lipid accumulation, however, the intensity of hepatic steatosis HF group was classified between 33 and 66% of the hepatic parenchyma with fat deposition. As for the area of adipocytes was no effect of diet, fruit or the interaction between the experimental groups. Supplementation with 2% lyophilized jussara açaí was not effective to improve the levels of AST, ALT, total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids and glucose. The jussara açaí did not change cardiovascular risk biomarcador ICAM, PAI-1 and e-selectin. In conclusion, supplementation with acai jussara was not effective in improving cardiovascular risk biomarcador and other biochemical parameters evaluated. On the other hand, acai jussara promoted reduction of hepatic steatosis of the mice subjected to high-fat diet.
id UFES_c9dacdc1853048e6337d4ac929423863
oai_identifier_str oai:repositorio.ufes.br:10/8105
network_acronym_str UFES
network_name_str Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
repository_id_str 2108
spelling Costa, Neuza Maria BrunoroOliveira, Daniela da SilvaCosta, André Gustavo VasconcelosSilva, Laíz Aparecida AzevedoNunes, Erika TakagiHermsdorff, Helen Hermana Miranda2018-08-01T23:19:05Z2018-08-012018-08-01T23:19:05Z2016-07-11Cardiovascular diseases (CVD) are the major responsible factor for morbidity and mortality rates worldwide. The intake of foods rich in antioxidants, such as acai, can help reducing the risk of CVD. The objective of this study was to investigate the effect of the jussara acai (Euterpe edulis Martius) on cardiovascular risk biomarcador in mice submitted to a high fat diet. The pulp of jussara acai was lyophilized and analysed for its chemical composition, phenolic compounds, anthocyanins and antioxidant capacity. For the biological assay, 43 adult male Swiss mice were used. Initially, the animals were submitted a control diet (AIN-93M) (n = 22) or high fat diet (n = 21) for 8 weeks. For a period of additional 8 weeks, the group received the control diet (AIN-93M): control diet (AIN-93M) (CT, n = 10) or control diet (AIN-93M) supplemented with 2% lyophilized jussara açai pulp (CT+A, n = 12); the group received high fat diet: high fat diet (HF, n = 9) or high fat diet supplemented with 2% lyophilized jussara acai pulp (HF+A, n = 12). At the end of the experiment the animals were euthanized and blood samples collected for analysis: aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids, glucose, activator inhibitor plasminogen type 1 (PAI-1), intercellular cell adhesion molecule (ICAM), e-selectin, were histologically analyzed liver and adipose tissue. The results were analyzed by t test to evaluate the control and HF groups prior to supplementation. The 'two-way' analysis of variance (ANOVA) was performed to evaluate the effect of diet and fruit and/or its interaction among the experimental groups (CT, HF, CT+A and HF+A). The data were presented as mean and standard error, with 5% significance level. The experimental groups CT, CT+A and HF+A had less than 33% of hepatocytes with lipid accumulation, however, the intensity of hepatic steatosis HF group was classified between 33 and 66% of the hepatic parenchyma with fat deposition. As for the area of adipocytes was no effect of diet, fruit or the interaction between the experimental groups. Supplementation with 2% lyophilized jussara açaí was not effective to improve the levels of AST, ALT, total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids and glucose. The jussara açaí did not change cardiovascular risk biomarcador ICAM, PAI-1 and e-selectin. In conclusion, supplementation with acai jussara was not effective in improving cardiovascular risk biomarcador and other biochemical parameters evaluated. On the other hand, acai jussara promoted reduction of hepatic steatosis of the mice subjected to high-fat diet.As doenças cardiovasculares (DCV) são as maiores responsáveis pelas taxas de morbimortalidade no mundo. A ingestão de alimentos ricos em compostos antioxidantes, como o açaí, pode contribuir para a redução do risco de DCV. O objetivo deste trabalho foi investigar o efeito do açaí da palmeira jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica. A polpa de açaí jussara foi liofilizada e avaliada quanto à sua composição centesimal, conteúdo de compostos fenólicos e antocianinas totais e sua capacidade antioxidante in vitro. Para o ensaio biológico, foram utilizados 43 camundongos machos adultos Swiss. Inicialmente, os animais foram submetidos a uma dieta normolipídica (n=22) ou dieta hiperlipídica (n=21), durante 8 semanas. Por um período de mais 8 semanas, o grupo em dieta normolipídica recebeu:dieta controle (AIN-93M) (CT, n=10) ou dieta controle (AIN-93M) suplementada com 2% de polpa do açaí jussara liofilizado (CT+A, n=12); o grupo em dieta hiperlipídica recebeu: dieta hiperlipídica (HF, n=9) ou dieta hiperlipídica suplementada com 2% de polpa do açaí jussara liofilizado (HF+A, n=12). Ao final do período experimental, os animais foram eutanasiados e colhidas amostras de sangue para análises de aspartato aminotransferase (AST), alanina aminotransferase (ALT), colesterol total, HDL-c, LDL-c, triacilgliceróis, ácidos graxos livres, glicose, inibidor do ativador do plasminogênio tipo 1 (PAI-1), molécula de adesão celular intercelular (ICAM) e e-selectina. Ainda, foram realizadas análises histológicas do fígado e do tecido adiposo. Os resultados foram analisados por o teste t para avaliar os grupos CT e HF, antes do período de suplementação. A análise de variância, Two-way, foi realizada para avaliar o efeito da dieta e do fruto e/ou suas interações entre os grupos experimentais (CT, HF, CT+A e HF+A). Os dados foram apresentados em média e erro padrão, com nível de significância de 5%. Os grupos experimentais CT, CT+A e HF+A apresentaram menos que 33% dos hepatócitos com acúmulo de lipídios, entretanto, a intensidade da esteatose hepática do grupo HF foi classificada entre 33 e 66% do parênquima hepático com deposição de gordura. Quanto à área dos adipócitos não foi observado efeito da dieta, fruto ou da interação entre os grupos experimentais. A suplementação com 2% de polpa de açaí jussara liofilizado não foi eficaz para melhorar os níveis de AST, ALT, colesterol total, HDL-c, LDL-c, triacilgliceróis, ácidos graxos livres e glicose. O açaí jussara não promoveu alterações nos marcadores de risco cardiovascular, ICAM, PAI-1 e e-selectina. Conclui-se que, a suplementação com açaí jussara não foi eficaz em melhorar os marcadores de risco cardiovascular e demais parâmetros bioquímicos avaliados. Por outro lado, o açaí jussara promoveu redução da esteatose hepática dos camundongos submetidos a dieta hiperlipídica.Texthttp://repositorio.ufes.br/handle/10/8105porUniversidade Federal do Espírito SantoMestrado em Ciência e Tecnologia de AlimentosPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosUFESBRCentro de Ciências Agrárias e EngenhariasAntioxidantCardiovascular healthAnthocyaninsHigh fat dietSaúde cardiovascularDieta hiperlipídicaAçaí jussaraCoração - DoençasSistema cardiovascular - DoençasAntioxidantesAntocianinasAçaíDietoterapiaCiência e Tecnologia de Alimentos664Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídicaEffect of jussara açaí (Euterpe edulis Martius) on markers risk cardiovascular in mice submitted to a high fat dietinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_9497_Resumo Dissertação Final - Laíz Aparecida Azevedo Silva.pdfapplication/pdf44729http://repositorio.ufes.br/bitstreams/19cf25d3-b3ee-4798-b840-25baedbd4634/downloadc9af6653fd453794e901414896b81fb2MD5110/81052024-06-21 16:56:13.084oai:repositorio.ufes.br:10/8105http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-07-11T14:34:25.455906Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
dc.title.alternative.none.fl_str_mv Effect of jussara açaí (Euterpe edulis Martius) on markers risk cardiovascular in mice submitted to a high fat diet
title Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
spellingShingle Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
Silva, Laíz Aparecida Azevedo
Antioxidant
Cardiovascular health
Anthocyanins
High fat diet
Saúde cardiovascular
Dieta hiperlipídica
Açaí jussara
Ciência e Tecnologia de Alimentos
Coração - Doenças
Sistema cardiovascular - Doenças
Antioxidantes
Antocianinas
Açaí
Dietoterapia
664
title_short Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
title_full Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
title_fullStr Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
title_full_unstemmed Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
title_sort Efeito do açai jussara (Euterpe edulis Martius) sobre marcadores de risco cardiovascular em camundongos submetidos a uma dieta hiperlipídica
author Silva, Laíz Aparecida Azevedo
author_facet Silva, Laíz Aparecida Azevedo
author_role author
dc.contributor.advisor-co1.fl_str_mv Costa, Neuza Maria Brunoro
dc.contributor.advisor-co2.fl_str_mv Oliveira, Daniela da Silva
dc.contributor.advisor1.fl_str_mv Costa, André Gustavo Vasconcelos
dc.contributor.author.fl_str_mv Silva, Laíz Aparecida Azevedo
dc.contributor.referee1.fl_str_mv Nunes, Erika Takagi
dc.contributor.referee2.fl_str_mv Hermsdorff, Helen Hermana Miranda
contributor_str_mv Costa, Neuza Maria Brunoro
Oliveira, Daniela da Silva
Costa, André Gustavo Vasconcelos
Nunes, Erika Takagi
Hermsdorff, Helen Hermana Miranda
dc.subject.eng.fl_str_mv Antioxidant
Cardiovascular health
Anthocyanins
High fat diet
topic Antioxidant
Cardiovascular health
Anthocyanins
High fat diet
Saúde cardiovascular
Dieta hiperlipídica
Açaí jussara
Ciência e Tecnologia de Alimentos
Coração - Doenças
Sistema cardiovascular - Doenças
Antioxidantes
Antocianinas
Açaí
Dietoterapia
664
dc.subject.por.fl_str_mv Saúde cardiovascular
Dieta hiperlipídica
Açaí jussara
dc.subject.cnpq.fl_str_mv Ciência e Tecnologia de Alimentos
dc.subject.br-rjbn.none.fl_str_mv Coração - Doenças
Sistema cardiovascular - Doenças
Antioxidantes
Antocianinas
Açaí
Dietoterapia
dc.subject.udc.none.fl_str_mv 664
description Cardiovascular diseases (CVD) are the major responsible factor for morbidity and mortality rates worldwide. The intake of foods rich in antioxidants, such as acai, can help reducing the risk of CVD. The objective of this study was to investigate the effect of the jussara acai (Euterpe edulis Martius) on cardiovascular risk biomarcador in mice submitted to a high fat diet. The pulp of jussara acai was lyophilized and analysed for its chemical composition, phenolic compounds, anthocyanins and antioxidant capacity. For the biological assay, 43 adult male Swiss mice were used. Initially, the animals were submitted a control diet (AIN-93M) (n = 22) or high fat diet (n = 21) for 8 weeks. For a period of additional 8 weeks, the group received the control diet (AIN-93M): control diet (AIN-93M) (CT, n = 10) or control diet (AIN-93M) supplemented with 2% lyophilized jussara açai pulp (CT+A, n = 12); the group received high fat diet: high fat diet (HF, n = 9) or high fat diet supplemented with 2% lyophilized jussara acai pulp (HF+A, n = 12). At the end of the experiment the animals were euthanized and blood samples collected for analysis: aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids, glucose, activator inhibitor plasminogen type 1 (PAI-1), intercellular cell adhesion molecule (ICAM), e-selectin, were histologically analyzed liver and adipose tissue. The results were analyzed by t test to evaluate the control and HF groups prior to supplementation. The 'two-way' analysis of variance (ANOVA) was performed to evaluate the effect of diet and fruit and/or its interaction among the experimental groups (CT, HF, CT+A and HF+A). The data were presented as mean and standard error, with 5% significance level. The experimental groups CT, CT+A and HF+A had less than 33% of hepatocytes with lipid accumulation, however, the intensity of hepatic steatosis HF group was classified between 33 and 66% of the hepatic parenchyma with fat deposition. As for the area of adipocytes was no effect of diet, fruit or the interaction between the experimental groups. Supplementation with 2% lyophilized jussara açaí was not effective to improve the levels of AST, ALT, total cholesterol, HDL-C, LDL-C, triglycerides, free fatty acids and glucose. The jussara açaí did not change cardiovascular risk biomarcador ICAM, PAI-1 and e-selectin. In conclusion, supplementation with acai jussara was not effective in improving cardiovascular risk biomarcador and other biochemical parameters evaluated. On the other hand, acai jussara promoted reduction of hepatic steatosis of the mice subjected to high-fat diet.
publishDate 2016
dc.date.issued.fl_str_mv 2016-07-11
dc.date.accessioned.fl_str_mv 2018-08-01T23:19:05Z
dc.date.available.fl_str_mv 2018-08-01
2018-08-01T23:19:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufes.br/handle/10/8105
url http://repositorio.ufes.br/handle/10/8105
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv Text
dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciência e Tecnologia de Alimentos
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
dc.publisher.initials.fl_str_mv UFES
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências Agrárias e Engenharias
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
instname:Universidade Federal do Espírito Santo (UFES)
instacron:UFES
instname_str Universidade Federal do Espírito Santo (UFES)
instacron_str UFES
institution UFES
reponame_str Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
collection Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
bitstream.url.fl_str_mv http://repositorio.ufes.br/bitstreams/19cf25d3-b3ee-4798-b840-25baedbd4634/download
bitstream.checksum.fl_str_mv c9af6653fd453794e901414896b81fb2
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)
repository.mail.fl_str_mv
_version_ 1813022612970799104