Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura

Detalhes bibliográficos
Autor(a) principal: Gasparini, Xismenia Soares da Silva
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/13885
Resumo: PPaubrasilia echinata is a tree species originating from the Atlantic Forest. This species has different morphotypes, among them the small and medium that differ in morphology and ecological habit. The small morphotype is described as an ombrophilous plant, while the medium morphotype shows heliophilic behavior. From the climate reports released, it is noted that environmental disturbances may occur as a result of these changes. According to reports, for the northeast portion of the Atlantic Forest is expected intense warming and reduction of rainfall. For the south / southeast portion, trends indicate warmer weather, with intensification in rainfall patterns. The objective of this study was to evaluate the combined effect of vapor pressure deficit (DPV) and temperature on carbon allocation, as well as non-enzymatic antioxidant responses (flavonoids, phenols and MDA) as mechanisms of tolerance to these climate changes. Therefore, plants of small and medium morphotypes were submitted to four different controlled environments (A), being A1- low DPV and temperature (0.7 KPa and 25.9 ° C); A2- low DPV and high temperature (31.6 ° C and 1.0 KPa); A3- high DPV and temperature (32.6 ° C and 3.1 KPa) and A4- high DPV and temperature (26 ° C and 1.7 KPa). The analysis of soluble sugars (AS), sucrose, reducing sugars (AR), starch and wall polymers (cellulose, hemicellulose and lignin) were performed in the three organs (leaf, stem and root) of plants of both morphotypes. Already the analyzes referring to the non- enzymatic oxidant system were performed in the leaves. Both morphotypes showed, in the A3 environment, higher concentration of leaf AS in relation to the stem and root. This result presented higher RA concentration and lower leaf sucrose concentration, since this carbohydrate can be used as osmoregulator, besides being the main translucent photoassimilate in plants and can be used in other reactions. The roots of the small morphotype, under elevated temperature, presented higher root concentration of sucrose compared to the other environments. In both morphotypes, the RA concentration was higher in leaves, especially in A3. Under high DPV, especially in A3, the small morphotype showed higher root starch concentration and lower leaf concentration. In the mean morphotype,. starch allocation was higher in leaves than in other organs. The highest starch concentration is related to storage organs (seeds, tubers, roots ...) and its lower leaf concentration is related to stress conditions. The high concentration of lignin in the roots of both morphotypes may favor water transport, besides protecting plant tissues from chemical / biological degradation. The highest concentration of cellulose and hemicellulose was observed in the medium morphotype A1 stems, and the opposite for the small morphotype. Regarding the oxidative reaction, the small morphotype presented higher content of H2O2 in A4, where the lipid peroxidation, indicated by the higher MDA content, was higher. The same result was observed for the average morphotype in A1. Under these conditions, the non enzymatic antioxidant system was inefficient due to the lower concentrations of phenols and flavonoids. In higher temperature environment, the small morphotype (A2) and the medium morphotype (A3) presented lower concentration of H2O2 and MDA. This result may suggest less cellular damage due to lower lipid peroxidation. Thus, it can be concluded that A1 and A4 appear to be stressful conditions for the small and medium morphotype, respectively. However A2 and A3 can be considered favorable environments, respectively, for the small and medium morphotypes.
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spelling Cuzzuol, Geraldo Rogerio Faustinihttps://orcid.org/http://lattes.cnpq.br/6127436626143032Gasparini, Xismenia Soares da Silvahttps://orcid.org/http://lattes.cnpq.br/Aquije, Gloria Maria de Farias Viegashttps://orcid.org/0000-0002-8810-7253http://lattes.cnpq.br/Milanez, Camilla Rozindo Diashttps://orcid.org/http://lattes.cnpq.br/1288447995978779Gomes, Marcos Thiago Gaudiohttps://orcid.org/http://lattes.cnpq.br/9903805899337418Cavatte, Paulo Cezarhttp://lattes.cnpq.br/80292799679504252024-05-30T00:48:44Z2024-05-30T00:48:44Z2019-11-06PPaubrasilia echinata is a tree species originating from the Atlantic Forest. This species has different morphotypes, among them the small and medium that differ in morphology and ecological habit. The small morphotype is described as an ombrophilous plant, while the medium morphotype shows heliophilic behavior. From the climate reports released, it is noted that environmental disturbances may occur as a result of these changes. According to reports, for the northeast portion of the Atlantic Forest is expected intense warming and reduction of rainfall. For the south / southeast portion, trends indicate warmer weather, with intensification in rainfall patterns. The objective of this study was to evaluate the combined effect of vapor pressure deficit (DPV) and temperature on carbon allocation, as well as non-enzymatic antioxidant responses (flavonoids, phenols and MDA) as mechanisms of tolerance to these climate changes. Therefore, plants of small and medium morphotypes were submitted to four different controlled environments (A), being A1- low DPV and temperature (0.7 KPa and 25.9 ° C); A2- low DPV and high temperature (31.6 ° C and 1.0 KPa); A3- high DPV and temperature (32.6 ° C and 3.1 KPa) and A4- high DPV and temperature (26 ° C and 1.7 KPa). The analysis of soluble sugars (AS), sucrose, reducing sugars (AR), starch and wall polymers (cellulose, hemicellulose and lignin) were performed in the three organs (leaf, stem and root) of plants of both morphotypes. Already the analyzes referring to the non- enzymatic oxidant system were performed in the leaves. Both morphotypes showed, in the A3 environment, higher concentration of leaf AS in relation to the stem and root. This result presented higher RA concentration and lower leaf sucrose concentration, since this carbohydrate can be used as osmoregulator, besides being the main translucent photoassimilate in plants and can be used in other reactions. The roots of the small morphotype, under elevated temperature, presented higher root concentration of sucrose compared to the other environments. In both morphotypes, the RA concentration was higher in leaves, especially in A3. Under high DPV, especially in A3, the small morphotype showed higher root starch concentration and lower leaf concentration. In the mean morphotype,. starch allocation was higher in leaves than in other organs. The highest starch concentration is related to storage organs (seeds, tubers, roots ...) and its lower leaf concentration is related to stress conditions. The high concentration of lignin in the roots of both morphotypes may favor water transport, besides protecting plant tissues from chemical / biological degradation. The highest concentration of cellulose and hemicellulose was observed in the medium morphotype A1 stems, and the opposite for the small morphotype. Regarding the oxidative reaction, the small morphotype presented higher content of H2O2 in A4, where the lipid peroxidation, indicated by the higher MDA content, was higher. The same result was observed for the average morphotype in A1. Under these conditions, the non enzymatic antioxidant system was inefficient due to the lower concentrations of phenols and flavonoids. In higher temperature environment, the small morphotype (A2) and the medium morphotype (A3) presented lower concentration of H2O2 and MDA. This result may suggest less cellular damage due to lower lipid peroxidation. Thus, it can be concluded that A1 and A4 appear to be stressful conditions for the small and medium morphotype, respectively. However A2 and A3 can be considered favorable environments, respectively, for the small and medium morphotypes.Paubrasilia echinata é uma espécie arbórea originária da Mata Atlântica. Essa espécie apresenta diferentes morfotipos, entre eles o pequeno e médio que se diferem na morfologia e hábito ecológico. O morfotipo pequeno é descrito como uma planta ombrófila, enquanto o morfotipo médio apresenta comportamento heliófilo. A partir dos relatórios climáticos divulgados, nota-se que poderão ocorrer perturbações ambientais decorrentes dessas mudanças. Segundo os relatórios, para porção nordeste da Mata Atlântica espera-se um aquecimento intenso e redução do índice pluviométrico. Já para a porção sul/sudeste, as tendências indicam clima mais quente, com intensificação nos padrões de chuva. Objetivou-se com este trabalho avaliar o efeito combinado de déficit de pressão de vapor (DPV) e temperatura na alocação de carbono, bem como as respostas antioxidantes não enzimáticas (flavonóides, fenóis e MDA), como mecanismos de tolerância a essas alterações climáticas. Para tanto, plantas dos morfotipos pequeno e médio foram submetidos a quatro diferentes ambientes (A) controlados, sendo A1- baixo DPV e temperatura (0,7 KPa e 25,9°C); A2- baixo DPV e alta temperatura (31,6°C e 1,0 KPa); A3- alto DPV e temperatura (32,6°C e 3,1 KPa) e A4- alto DPV e baixa temperatura (26°C e 1,7 KPa). As análises de açúcares solúveis (AS), sacarose, açúcares redutores (AR), amido e polímeros de parede (celulose, hemicelulose e lignina) foram realizados nos três órgãos (folha, caule e raiz) de plantas dos dois morfotipos. Já as análises referentes ao sistema oxidante não enzimático foram realizadas nas folhas. Ambos os morfotipos demostraram, no ambiente A3, maior concentração de AS foliar em relação ao caule e raiz. Esse resultado apresentou maior concentração de AR e menor concentração de sacarose foliar, uma vez que esse carboidrato pode ser utilizado como osmorregulador, além de ser o principal fotoassimilado translocado em plantas, podendo ser utilizado em outras reações. As raízes do morfotipo pequeno, sob temperatura elevada, apresentaram maior concentração radicular de sacarose em comparação com aos demais ambientes. Observou-se que, nos dois morfotipos, a concentração de AR foi superior em folhas, principalmente em A3. Sob alto DPV, principalmente em A3, o morfotipo pequeno apresentou maior concentração de amido radicular e menor concentração nas folhas. Já no morfotipo médio, a alocação de amido foi maior nas folhas em relação aos demais órgãos. A maior concentração de amido está relacionada a órgãos de armazenamento (sementes, tubérculos, raízes...) e sua menor concentração foliar está relacionada a condições de estresse. Já a elevada concentração de lignina nas raízes de ambos os morfotipos pode favorecer o transporte de água, além de proteger os tecidos vegetais de degradação quimica/biológica. A maior concentração de celulose e hemicelulose foi observada nos caules de A1 do morfotipo médio, sendo o inverso para o morfotipo pequeno. Quanto á reação oxidante, o morfotipo pequeno apresentou maior teor de H2O2 em A4, onde a peroxidação lipídica, indicada pelo maior teor de MDA, foi maior. O mesmo resultado foi observado para o morfotipo médio no A1. Nessas condições, o sistema antioxidante não enzimático mostrou-se ineficiente devido às menores concentrações de fenóis e flavonóides. Em ambiente de maior temperatura, o morfotipo pequeno (A2) e o morfotipo médio (A3) apresentaram menor concentração de H2O2 e MDA. Esse resultado pode sugerir menor dano celular, devido a menor peroxidação lipídica. Sendo assim, pode-se concluir que A1 e A4 aparentam ser condições estressantes para o morfotipo pequeno e médio, respectivamente. Entretanto A2 e A3 podem ser considerados ambientes favoráveis, respectivamente, para os morfotipos pequeno e médioTexthttp://repositorio.ufes.br/handle/10/13885porUniversidade Federal do Espírito SantoDoutorado em Biologia VegetalPrograma de Pós-Graduação em Biologia VegetalUFESBRCentro de Ciências Humanas e Naturaissubject.br-rjbnAgronomiaPau-brasil DPVDPVDPVTemperaturaTemperaturaTemperaturaCarboidratosCarboidratosCarboidratosERO’sERO’sERO’sBrazil woodTemperatureCarbohydratesAlocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperaturatitle.alternativeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALXismeniaSoaresdaSilvaGasparini-2019-Tese.pdfapplication/pdf1662307http://repositorio.ufes.br/bitstreams/9f44da52-8723-4021-a54e-9b797c928786/download58cc3827576ac548d1254bc76579497fMD5110/138852024-07-25 00:09:58.998oai:repositorio.ufes.br:10/13885http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T18:00:43.150996Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
dc.title.alternative.none.fl_str_mv title.alternative
title Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
spellingShingle Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
Gasparini, Xismenia Soares da Silva
Agronomia
Pau-brasil
DPV
DPV
DPV
Temperatura
Temperatura
Temperatura
Carboidratos
Carboidratos
Carboidratos
ERO’s
ERO’s
ERO’s
Brazil wood
Temperature
Carbohydrates
subject.br-rjbn
title_short Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
title_full Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
title_fullStr Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
title_full_unstemmed Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
title_sort Alocação de carbono e resposta oxidante em dois morfotipos de Paubrasilia echinata submetidos a diferentes condições de déficit de pressão de vapor e temperatura
author Gasparini, Xismenia Soares da Silva
author_facet Gasparini, Xismenia Soares da Silva
author_role author
dc.contributor.authorID.none.fl_str_mv https://orcid.org/
dc.contributor.authorLattes.none.fl_str_mv http://lattes.cnpq.br/
dc.contributor.advisor1.fl_str_mv Cuzzuol, Geraldo Rogerio Faustini
dc.contributor.advisor1ID.fl_str_mv https://orcid.org/
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6127436626143032
dc.contributor.author.fl_str_mv Gasparini, Xismenia Soares da Silva
dc.contributor.referee1.fl_str_mv Aquije, Gloria Maria de Farias Viegas
dc.contributor.referee1ID.fl_str_mv https://orcid.org/0000-0002-8810-7253
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/
dc.contributor.referee2.fl_str_mv Milanez, Camilla Rozindo Dias
dc.contributor.referee2ID.fl_str_mv https://orcid.org/
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1288447995978779
dc.contributor.referee3.fl_str_mv Gomes, Marcos Thiago Gaudio
dc.contributor.referee3ID.fl_str_mv https://orcid.org/
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/9903805899337418
dc.contributor.referee4.fl_str_mv Cavatte, Paulo Cezar
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/8029279967950425
contributor_str_mv Cuzzuol, Geraldo Rogerio Faustini
Aquije, Gloria Maria de Farias Viegas
Milanez, Camilla Rozindo Dias
Gomes, Marcos Thiago Gaudio
Cavatte, Paulo Cezar
dc.subject.cnpq.fl_str_mv Agronomia
topic Agronomia
Pau-brasil
DPV
DPV
DPV
Temperatura
Temperatura
Temperatura
Carboidratos
Carboidratos
Carboidratos
ERO’s
ERO’s
ERO’s
Brazil wood
Temperature
Carbohydrates
subject.br-rjbn
dc.subject.por.fl_str_mv Pau-brasil
DPV
DPV
DPV
Temperatura
Temperatura
Temperatura
Carboidratos
Carboidratos
Carboidratos
ERO’s
ERO’s
ERO’s
Brazil wood
Temperature
Carbohydrates
dc.subject.br-rjbn.none.fl_str_mv subject.br-rjbn
description PPaubrasilia echinata is a tree species originating from the Atlantic Forest. This species has different morphotypes, among them the small and medium that differ in morphology and ecological habit. The small morphotype is described as an ombrophilous plant, while the medium morphotype shows heliophilic behavior. From the climate reports released, it is noted that environmental disturbances may occur as a result of these changes. According to reports, for the northeast portion of the Atlantic Forest is expected intense warming and reduction of rainfall. For the south / southeast portion, trends indicate warmer weather, with intensification in rainfall patterns. The objective of this study was to evaluate the combined effect of vapor pressure deficit (DPV) and temperature on carbon allocation, as well as non-enzymatic antioxidant responses (flavonoids, phenols and MDA) as mechanisms of tolerance to these climate changes. Therefore, plants of small and medium morphotypes were submitted to four different controlled environments (A), being A1- low DPV and temperature (0.7 KPa and 25.9 ° C); A2- low DPV and high temperature (31.6 ° C and 1.0 KPa); A3- high DPV and temperature (32.6 ° C and 3.1 KPa) and A4- high DPV and temperature (26 ° C and 1.7 KPa). The analysis of soluble sugars (AS), sucrose, reducing sugars (AR), starch and wall polymers (cellulose, hemicellulose and lignin) were performed in the three organs (leaf, stem and root) of plants of both morphotypes. Already the analyzes referring to the non- enzymatic oxidant system were performed in the leaves. Both morphotypes showed, in the A3 environment, higher concentration of leaf AS in relation to the stem and root. This result presented higher RA concentration and lower leaf sucrose concentration, since this carbohydrate can be used as osmoregulator, besides being the main translucent photoassimilate in plants and can be used in other reactions. The roots of the small morphotype, under elevated temperature, presented higher root concentration of sucrose compared to the other environments. In both morphotypes, the RA concentration was higher in leaves, especially in A3. Under high DPV, especially in A3, the small morphotype showed higher root starch concentration and lower leaf concentration. In the mean morphotype,. starch allocation was higher in leaves than in other organs. The highest starch concentration is related to storage organs (seeds, tubers, roots ...) and its lower leaf concentration is related to stress conditions. The high concentration of lignin in the roots of both morphotypes may favor water transport, besides protecting plant tissues from chemical / biological degradation. The highest concentration of cellulose and hemicellulose was observed in the medium morphotype A1 stems, and the opposite for the small morphotype. Regarding the oxidative reaction, the small morphotype presented higher content of H2O2 in A4, where the lipid peroxidation, indicated by the higher MDA content, was higher. The same result was observed for the average morphotype in A1. Under these conditions, the non enzymatic antioxidant system was inefficient due to the lower concentrations of phenols and flavonoids. In higher temperature environment, the small morphotype (A2) and the medium morphotype (A3) presented lower concentration of H2O2 and MDA. This result may suggest less cellular damage due to lower lipid peroxidation. Thus, it can be concluded that A1 and A4 appear to be stressful conditions for the small and medium morphotype, respectively. However A2 and A3 can be considered favorable environments, respectively, for the small and medium morphotypes.
publishDate 2019
dc.date.issued.fl_str_mv 2019-11-06
dc.date.accessioned.fl_str_mv 2024-05-30T00:48:44Z
dc.date.available.fl_str_mv 2024-05-30T00:48:44Z
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dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Doutorado em Biologia Vegetal
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biologia Vegetal
dc.publisher.initials.fl_str_mv UFES
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dc.publisher.department.fl_str_mv Centro de Ciências Humanas e Naturais
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Doutorado em Biologia Vegetal
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