DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
Texto Completo: | https://app.uff.br/riuff/handle/1/18272 |
Resumo: | Introduction: Neuron-specific enolase (NSE) is a glycolytic enzyme involved in the glycolysis pathway. Assays to evaluate the presence of NSE in lung cancer have been widely investigated and its presence defines neuroendocrine differentiation. However, their diagnostic values have not yet been clarified in pleural fluids (PFs). Objective: To determine the usefulness of NSE in PFs (L-NSE) in malign pleural effusion and its differentiation from benign effusions. Method: Time-resolved immunofluorometric assay (TRF). Results: The enzyme levels were evaluated in seventy patients with benign and malignant PFs. Twenty patients with malignant pleurisy due to non-small cell lung cancer or NSCLC (n=15), lymphomas (n=4) and neuroblastoma (n=1). Fifty control patients with benign PFs with miscellaneous causes, mainly tuberculosis (33.0%). The group of malign pleural effusion was 9 men and 11 women with average age of 67. 6 years (35 to 92 years). Benign PFs were comprised of 30 men and 20 women with an average age of 49 years (3 to 94 years). The mean level of L-NSE was higher in malign pleural effusions (38.5 ± 54.9µg/L) than in patients with benign pleural effusions (9.35 ± 23.7µg/L). Two tail p value was statistically significant (p=0.0304). Kruskal-Wallis test with post hoc procedures compared three unpaired groups. It was statistically significant (H=9.998; p=0.0067). Post-Hoc Dunn's test revealed a significant difference of benign group versus NSCLC group (p<0.01) but not lymphomas versus benign group (p>0.05) and lymphomas versus NSCLC (p>0.05). L-NSE levels 28,5 µg/L in malignant pleurisy showed a sensitivity of 30,0 %, a specificity of 96,0 %, a positive predictive value of 75,0 %, a negative predictive value of 77,0 %, a positive likelihood of 7,5 a negative likelihood of 0,32 and a test accuracy of 77,1. Conclusion: L-NSE is useful to differentiate malignant from benign pleurisy. |
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DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICADiagnosis differentiation Neuroendocrine in pleural fluid via molecular marker Cytophasmic enolase neuron specificFosfopiruvato hidrataseDerrame pleuralMarcador biológico de tumorFosfopiruvatobiological marker of tumorpleural effusionsCNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIAIntroduction: Neuron-specific enolase (NSE) is a glycolytic enzyme involved in the glycolysis pathway. Assays to evaluate the presence of NSE in lung cancer have been widely investigated and its presence defines neuroendocrine differentiation. However, their diagnostic values have not yet been clarified in pleural fluids (PFs). Objective: To determine the usefulness of NSE in PFs (L-NSE) in malign pleural effusion and its differentiation from benign effusions. Method: Time-resolved immunofluorometric assay (TRF). Results: The enzyme levels were evaluated in seventy patients with benign and malignant PFs. Twenty patients with malignant pleurisy due to non-small cell lung cancer or NSCLC (n=15), lymphomas (n=4) and neuroblastoma (n=1). Fifty control patients with benign PFs with miscellaneous causes, mainly tuberculosis (33.0%). The group of malign pleural effusion was 9 men and 11 women with average age of 67. 6 years (35 to 92 years). Benign PFs were comprised of 30 men and 20 women with an average age of 49 years (3 to 94 years). The mean level of L-NSE was higher in malign pleural effusions (38.5 ± 54.9µg/L) than in patients with benign pleural effusions (9.35 ± 23.7µg/L). Two tail p value was statistically significant (p=0.0304). Kruskal-Wallis test with post hoc procedures compared three unpaired groups. It was statistically significant (H=9.998; p=0.0067). Post-Hoc Dunn's test revealed a significant difference of benign group versus NSCLC group (p<0.01) but not lymphomas versus benign group (p>0.05) and lymphomas versus NSCLC (p>0.05). L-NSE levels 28,5 µg/L in malignant pleurisy showed a sensitivity of 30,0 %, a specificity of 96,0 %, a positive predictive value of 75,0 %, a negative predictive value of 77,0 %, a positive likelihood of 7,5 a negative likelihood of 0,32 and a test accuracy of 77,1. Conclusion: L-NSE is useful to differentiate malignant from benign pleurisy.Introdução: Enolase neurônio-específica (NSE) é uma enzima envolvida na via glicolítica e seus níveis séricos têm sido analisados nos carcinomas de pulmão como marcador de diferenciação neuroendócrina. Entretanto, sua dosagem nos líquidos pleurais ainda não é utilizada como método diagnóstico. Objetivo principal: Contribuir com o estudo da enzima enolase neurônio-específica pleural (NSE-L) utilizando sua dosagem para diagnóstico diferencial entre derrame pleural benigno e maligno. MÉTODO: Imunofluorometria a tempo resolvido (TRF). RESULTADOS: NSE-L foi dosada em 70 pacientes: 20 pacientes com derrames pleurais malignos devido a NSCLC ou carcinoma brônquico de não-pequenas células (n=15), linfomas (n=4) e neuroblastoma (n=1). 50 pacientes (grupo controle) com derrames pleurais benignos por várias causas, principalmente tuberculose (33%). O grupo com malignidade foi constituído por 9 homens com uma média de idade de 67,6 anos (35 92) e o grupo controle por 30 homens com uma média de idade de 49 anos (3 94). A média de dosagens de NSE-L foi mais elevada no grupo maligno (38,5 ± 59,9 µg/L) do que no grupo benigno (9,35 ± 23,7 µg/L) com um valor de p bicaudal igual a 0,0304). A comparação multivariada pelo teste de Kruskal- Wallis foi estatisticamente significante (H=9,998, p=0,0067). O teste post-hoc de Dunn calculou uma diferença estatisticamente significativa entre as doenças benignas e NSCLC (p<0,01), mas não entre linfomas versus NSCLC (p>0,05) e linfomas versus doenças benignas (p>0,05). Os parâmetros diagnósticos do exame da NSE-L, com valor de referência calculado de 28,5 µg/L, para o diagnóstico de derrame pleural maligno foi sensibilidade de 30,0%; especificidade de 96,0 %; valor preditivo positivo de 75,0%; valor preditivo negativo de 77,0 %; eficiência de 77,1 %; razão de verossimilhança positiva de 7,5 e razão de verossimilhança negativa de 0,32. CONCLUSÕES: A dosagem de NSE-L apresenta potencial bioquímico para diferenciar derrames pleurais malignos e benignos.Programa de Pós-graduação em NeuroimunologiaNeuroimunologiaAraújo, Elizabeth Giestal deCPF:55544433122http://lattes.cnpq.br/8230334072312477Carvalho, Roberto Paes deCPF:88867757722http://lattes.cnpq.br/6093972827853331Cardoso, Gilberto PerezCPF:95234953622http://lattes.cnpq.br/3949041976687209Saito, Eduardo HaruoCPF:89658447222http://lattes.cnpq.br/7735240966958450Lopes, Agnaldo JoséCPF:00089879822http://lattes.cnpq.br/1548788734980219Silva Junior, Cyro Teixeira da2021-03-10T20:44:05Z2009-06-052021-03-10T20:44:05Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/18272porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:44:05Zoai:app.uff.br:1/18272Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T11:15:40.078800Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA Diagnosis differentiation Neuroendocrine in pleural fluid via molecular marker Cytophasmic enolase neuron specific |
title |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA |
spellingShingle |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA Silva Junior, Cyro Teixeira da Fosfopiruvato hidratase Derrame pleural Marcador biológico de tumor Fosfopiruvato biological marker of tumor pleural effusions CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA |
title_short |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA |
title_full |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA |
title_fullStr |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA |
title_full_unstemmed |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA |
title_sort |
DIAGNÓSTICO DE DIFERENCIAÇÃO NEUROENDÓCRINA NO LÍQUIDO PLEURAL ATRAVÉS DO MARCADOR MOLECULAR CITOPLASMÁTICO ENOLASE NEURÔNIO ESPECÍFICA |
author |
Silva Junior, Cyro Teixeira da |
author_facet |
Silva Junior, Cyro Teixeira da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Araújo, Elizabeth Giestal de CPF:55544433122 http://lattes.cnpq.br/8230334072312477 Carvalho, Roberto Paes de CPF:88867757722 http://lattes.cnpq.br/6093972827853331 Cardoso, Gilberto Perez CPF:95234953622 http://lattes.cnpq.br/3949041976687209 Saito, Eduardo Haruo CPF:89658447222 http://lattes.cnpq.br/7735240966958450 Lopes, Agnaldo José CPF:00089879822 http://lattes.cnpq.br/1548788734980219 |
dc.contributor.author.fl_str_mv |
Silva Junior, Cyro Teixeira da |
dc.subject.por.fl_str_mv |
Fosfopiruvato hidratase Derrame pleural Marcador biológico de tumor Fosfopiruvato biological marker of tumor pleural effusions CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA |
topic |
Fosfopiruvato hidratase Derrame pleural Marcador biológico de tumor Fosfopiruvato biological marker of tumor pleural effusions CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA |
description |
Introduction: Neuron-specific enolase (NSE) is a glycolytic enzyme involved in the glycolysis pathway. Assays to evaluate the presence of NSE in lung cancer have been widely investigated and its presence defines neuroendocrine differentiation. However, their diagnostic values have not yet been clarified in pleural fluids (PFs). Objective: To determine the usefulness of NSE in PFs (L-NSE) in malign pleural effusion and its differentiation from benign effusions. Method: Time-resolved immunofluorometric assay (TRF). Results: The enzyme levels were evaluated in seventy patients with benign and malignant PFs. Twenty patients with malignant pleurisy due to non-small cell lung cancer or NSCLC (n=15), lymphomas (n=4) and neuroblastoma (n=1). Fifty control patients with benign PFs with miscellaneous causes, mainly tuberculosis (33.0%). The group of malign pleural effusion was 9 men and 11 women with average age of 67. 6 years (35 to 92 years). Benign PFs were comprised of 30 men and 20 women with an average age of 49 years (3 to 94 years). The mean level of L-NSE was higher in malign pleural effusions (38.5 ± 54.9µg/L) than in patients with benign pleural effusions (9.35 ± 23.7µg/L). Two tail p value was statistically significant (p=0.0304). Kruskal-Wallis test with post hoc procedures compared three unpaired groups. It was statistically significant (H=9.998; p=0.0067). Post-Hoc Dunn's test revealed a significant difference of benign group versus NSCLC group (p<0.01) but not lymphomas versus benign group (p>0.05) and lymphomas versus NSCLC (p>0.05). L-NSE levels 28,5 µg/L in malignant pleurisy showed a sensitivity of 30,0 %, a specificity of 96,0 %, a positive predictive value of 75,0 %, a negative predictive value of 77,0 %, a positive likelihood of 7,5 a negative likelihood of 0,32 and a test accuracy of 77,1. Conclusion: L-NSE is useful to differentiate malignant from benign pleurisy. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-05 2021-03-10T20:44:05Z 2021-03-10T20:44:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/18272 |
url |
https://app.uff.br/riuff/handle/1/18272 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Neuroimunologia Neuroimunologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Neuroimunologia Neuroimunologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
instacron_str |
UFF |
institution |
UFF |
reponame_str |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
collection |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
repository.mail.fl_str_mv |
riuff@id.uff.br |
_version_ |
1811823705503825920 |