FGFb AUMENTA A PROLIFERAÇÃO DE CÉLULAS DA RETINA EM CULTURA: VIAS DE SINALIZAÇÃO ENVOLVIDAS

• Autor(a) principal: Ferraz, Carla Valéria Vieira Guilarducci
• Data de Publicação: 2009
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
• Texto Completo: https://app.uff.br/riuff/handle/1/18439
• topic: Fator de crescimento de fribroblasto básico; Proliferação; Tirosina cinase; P13 cinase; Proteína cinase C; Retina animal; Proliferação celular; Growth factor of basic fribroblasto; Proliferation; Tyrosine kinase, P13 kinase, protein kinase C; CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA

Basic fibroblast growth factor (bFGF) plays a central role during the development of the nervous system. It was already demonstrated the effect of bFGF on retinal cell proliferation. However, the intracellular pathways involved in the proliferative effect are still unknown. In the present work we analyzed the intracellular pathways involved in the increase of cell proliferation induced by bFGF treatment (25 and 50ng/mL). Our results clearly show that the bFGF effect is dose, time and plating density dependent. 50ng/mL bFGF induced the greatest effect (150% increase compared to the control), however none of the kinase inhibitors used in our study, were able to blocked the effect. Treatment with 25ng/mL bFGF induced a 100% increase in cell proliferation. This effect was blocked by inhibitors of tyrosine kinases, PI3 kinase and by simultaneous treatment with PKC and PI3 kinase inhibitors. Our results also show that bFGF induces a transient increase in the expression o phosphoAKT. We observed the greatest effect after 2h bFGF treatment. Our results indicate that bFGF plays an important role controlling the proliferative state of neonatal retinal cells.