Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais

Detalhes bibliográficos
Autor(a) principal: Sá, Maria de Fátima Leal Manhães de
Data de Publicação: 2007
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
Texto Completo: https://app.uff.br/riuff/handle/1/17631
Resumo: The aim of this work is to evaluate the presence of Charcot-Leyden crystals in stool specimens as an auxiliary reliable laboratorial diagnostic method and to establish a methodological protocol of parasitological exam of feces to determine the presence of enteroparasitosis. Stool and blood samples of two distinct populations were analyzed in order to determine the prevalence. The first group was composed of 168 children (of both sexes and between 2 and 5 years and 11 months old) who are enrolled in public daycare centers in Niterói, in the state of Rio de Janeiro. The second group was composed of 369 patients from the Antônio Pedro University hospital (HUAP). All exams from both groups were analyzed in the same period, in the Hematology and Parasitology Laboratories of HUAP. The stool specimens from the children were collected in labeled plastic vials with 10% buffered formaldehyde and processed through the standard Coprotest method. Specific white cell count was determined from the blood that was collected by finger prick with appropriate lancet. The blood smear was prepared immediately, stained with Wright stain and subsequently analyzed under a optic microscope with a 1000 fold magnification. The counts were undertaken with the help of a differential cell counter. The blood samples of the outpatients from HUAP were processed in the hospitals Hematology Laboratory, in an automatic cell counter. The parasitological exams of the stool specimens of both outpatients and children were processed by routine methods used in HUAP s Parasitology Laboratory. In short, smears were prepared as of the stool samples and stained subsequently with Wright stain to search for the presence of Charcot-Leyden Crystals. The stool smears were analyzed under an optic microscope with a 1000 fold increase to determine the presence of enteroparasitosis and Charcot-Leyden Crystals. The presence of eosinophils in the blood smears was searched to establish a possible correlation between the findings in the studied populations. The most frequent intestinal parasites encountered in the daycare center population were: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lambia (8%) while in HUAP´s outpatient population were: Entamoeba coli (15%), Giardia lambia (11%), Strongyloides stercoralis (6%) and Blastocystis hominis (5%). The presence of Charcot-Leyden Crystals showed to be a quick, low cost and highly efficient method in the aid of parasitological exam in stool specimens, when associated to the presence of eosinophilia in the peripheral blood. The detection of these pathogenic enteroparasites suggests poor hygiene conditions of the studied populations
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spelling Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinaisCristais de Charcot-LeydenEosinofiliaEnteroparasitosesEnteropatia parasitáriaTécnica e procedimento de laboratórioFezesParasitologiaPatologia ClínicaMedicinaCharcot-Leyden CrystalsEosinophilia: EnteroparasitosisCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICAThe aim of this work is to evaluate the presence of Charcot-Leyden crystals in stool specimens as an auxiliary reliable laboratorial diagnostic method and to establish a methodological protocol of parasitological exam of feces to determine the presence of enteroparasitosis. Stool and blood samples of two distinct populations were analyzed in order to determine the prevalence. The first group was composed of 168 children (of both sexes and between 2 and 5 years and 11 months old) who are enrolled in public daycare centers in Niterói, in the state of Rio de Janeiro. The second group was composed of 369 patients from the Antônio Pedro University hospital (HUAP). All exams from both groups were analyzed in the same period, in the Hematology and Parasitology Laboratories of HUAP. The stool specimens from the children were collected in labeled plastic vials with 10% buffered formaldehyde and processed through the standard Coprotest method. Specific white cell count was determined from the blood that was collected by finger prick with appropriate lancet. The blood smear was prepared immediately, stained with Wright stain and subsequently analyzed under a optic microscope with a 1000 fold magnification. The counts were undertaken with the help of a differential cell counter. The blood samples of the outpatients from HUAP were processed in the hospitals Hematology Laboratory, in an automatic cell counter. The parasitological exams of the stool specimens of both outpatients and children were processed by routine methods used in HUAP s Parasitology Laboratory. In short, smears were prepared as of the stool samples and stained subsequently with Wright stain to search for the presence of Charcot-Leyden Crystals. The stool smears were analyzed under an optic microscope with a 1000 fold increase to determine the presence of enteroparasitosis and Charcot-Leyden Crystals. The presence of eosinophils in the blood smears was searched to establish a possible correlation between the findings in the studied populations. The most frequent intestinal parasites encountered in the daycare center population were: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lambia (8%) while in HUAP´s outpatient population were: Entamoeba coli (15%), Giardia lambia (11%), Strongyloides stercoralis (6%) and Blastocystis hominis (5%). The presence of Charcot-Leyden Crystals showed to be a quick, low cost and highly efficient method in the aid of parasitological exam in stool specimens, when associated to the presence of eosinophilia in the peripheral blood. The detection of these pathogenic enteroparasites suggests poor hygiene conditions of the studied populationsO presente trabalho tem como objetivo avaliar a pesquisa de Cristais de Charcort-Leyden nas fezes, como método de diagnóstico laboratorial auxiliar de enteroparasitoses e estabelecer um protocolo metodológico de exame parasitológico de fezes para o diagnóstico das enteroparasitoses, com alta confiabilidade. Foi feito um estudo de prevalência em duas populações distintas. Foram analisadas amostras fecais e sangüíneas de 168 crianças de ambos os sexos, com faixa etária de 2 anos até 5 anos e 11 meses matriculadas em creches municipais e/ou municipalizadas de Niterói-RJ, e de 369 pacientes atendidos no HUAP, que tinham exames a serem analisados na mesma data, nos Laboratórios de Hematologia e Parasitologia do HUAP. Nas creches estudadas as fezes foram colhidas em frascos contendo formol tamponado 10% e processadas pelo método padronizado Coprotest. A contagem da série branca específica foi determinada a partir do sangue colhido de picada de dedo com lanceta apropriada. Foram preparados esfregaços sangüíneos posteriormente corados pelo Wright. Os esfregaços foram analisados ao microscópio óptico com aumento de 1000X e a contagem foi feita com auxílio de contador diferencial de células. As amostras de sangue dos pacientes atendidos no HUAP foram processadas no Laboratório de Hematologia do HUAP, em aparelho de automação. O exame parasitológico de fezes foi realizado no Laboratório de Parasitologia do HUAP. Ambos os exames dos pacientes atendidos no HUAP, foram realizados através de métodos utilizados segundo protocolo dos laboratórios. Com as amostras fecais de ambas populações estudadas, foram feitos esfregaços estirados e corados posteriormente com Wright para pesquisar Cristais de Charcot-Leyden. Esses esfregaços foram analisados em microscópio óptico com aumento 1000X. Foram analisadas a prevalência das enteroparasitoses, presença de Cristais de Charcot-Leyden nas fezes, eosinofilia sangüínea e a correlação destes ítens citados nas populações estudadas. Os parasitos intestinais mais freqüentes nas creches foram: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lamblia (8%). No HUAP foram: Entamoeba coli (15%), Giardia lamblia (11%), Strongyloides stercoralis (6%) e Blastocystis hominis (5%). A detecção desses enteroparasitos patogênicos sugere deficiência na higiene das populações estudadas. Para diagnostica-las, a pesquisa de cristais de Charcot-Leyden nas fezes mostrou ser um método rápido, de baixo custo e boa eficácia para auxiliar o exame parasitológico de fezes, associado à avaliação de eosinofilia sanguíneaPrograma de Pós-graduação em PatologiaPatologiaMacedo, Heloisa Werneck deCPF:99124122734http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707043E6Kang, Hye ChungCPF:00788511122http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4775656D2Mondino, Silvia Susana Bona deCPF:59567546720http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707023A6Reis, Neuzir TrindadeCPF:77800563422Sá, Maria de Fátima Leal Manhães de2021-03-10T20:42:17Z2008-01-112021-03-10T20:42:17Z2007-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/17631porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:42:17Zoai:app.uff.br:1/17631Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T10:47:06.849383Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false
dc.title.none.fl_str_mv Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
title Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
spellingShingle Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
Sá, Maria de Fátima Leal Manhães de
Cristais de Charcot-Leyden
Eosinofilia
Enteroparasitoses
Enteropatia parasitária
Técnica e procedimento de laboratório
Fezes
Parasitologia
Patologia Clínica
Medicina
Charcot-Leyden Crystals
Eosinophilia: Enteroparasitosis
CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA
title_short Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
title_full Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
title_fullStr Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
title_full_unstemmed Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
title_sort Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
author Sá, Maria de Fátima Leal Manhães de
author_facet Sá, Maria de Fátima Leal Manhães de
author_role author
dc.contributor.none.fl_str_mv Macedo, Heloisa Werneck de
CPF:99124122734
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707043E6
Kang, Hye Chung
CPF:00788511122
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4775656D2
Mondino, Silvia Susana Bona de
CPF:59567546720
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707023A6
Reis, Neuzir Trindade
CPF:77800563422
dc.contributor.author.fl_str_mv Sá, Maria de Fátima Leal Manhães de
dc.subject.por.fl_str_mv Cristais de Charcot-Leyden
Eosinofilia
Enteroparasitoses
Enteropatia parasitária
Técnica e procedimento de laboratório
Fezes
Parasitologia
Patologia Clínica
Medicina
Charcot-Leyden Crystals
Eosinophilia: Enteroparasitosis
CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA
topic Cristais de Charcot-Leyden
Eosinofilia
Enteroparasitoses
Enteropatia parasitária
Técnica e procedimento de laboratório
Fezes
Parasitologia
Patologia Clínica
Medicina
Charcot-Leyden Crystals
Eosinophilia: Enteroparasitosis
CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA
description The aim of this work is to evaluate the presence of Charcot-Leyden crystals in stool specimens as an auxiliary reliable laboratorial diagnostic method and to establish a methodological protocol of parasitological exam of feces to determine the presence of enteroparasitosis. Stool and blood samples of two distinct populations were analyzed in order to determine the prevalence. The first group was composed of 168 children (of both sexes and between 2 and 5 years and 11 months old) who are enrolled in public daycare centers in Niterói, in the state of Rio de Janeiro. The second group was composed of 369 patients from the Antônio Pedro University hospital (HUAP). All exams from both groups were analyzed in the same period, in the Hematology and Parasitology Laboratories of HUAP. The stool specimens from the children were collected in labeled plastic vials with 10% buffered formaldehyde and processed through the standard Coprotest method. Specific white cell count was determined from the blood that was collected by finger prick with appropriate lancet. The blood smear was prepared immediately, stained with Wright stain and subsequently analyzed under a optic microscope with a 1000 fold magnification. The counts were undertaken with the help of a differential cell counter. The blood samples of the outpatients from HUAP were processed in the hospitals Hematology Laboratory, in an automatic cell counter. The parasitological exams of the stool specimens of both outpatients and children were processed by routine methods used in HUAP s Parasitology Laboratory. In short, smears were prepared as of the stool samples and stained subsequently with Wright stain to search for the presence of Charcot-Leyden Crystals. The stool smears were analyzed under an optic microscope with a 1000 fold increase to determine the presence of enteroparasitosis and Charcot-Leyden Crystals. The presence of eosinophils in the blood smears was searched to establish a possible correlation between the findings in the studied populations. The most frequent intestinal parasites encountered in the daycare center population were: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lambia (8%) while in HUAP´s outpatient population were: Entamoeba coli (15%), Giardia lambia (11%), Strongyloides stercoralis (6%) and Blastocystis hominis (5%). The presence of Charcot-Leyden Crystals showed to be a quick, low cost and highly efficient method in the aid of parasitological exam in stool specimens, when associated to the presence of eosinophilia in the peripheral blood. The detection of these pathogenic enteroparasites suggests poor hygiene conditions of the studied populations
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
2008-01-11
2021-03-10T20:42:17Z
2021-03-10T20:42:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://app.uff.br/riuff/handle/1/17631
url https://app.uff.br/riuff/handle/1/17631
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language por
dc.rights.driver.fl_str_mv CC-BY-SA
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC-BY-SA
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Programa de Pós-graduação em Patologia
Patologia
publisher.none.fl_str_mv Programa de Pós-graduação em Patologia
Patologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)
instname:Universidade Federal Fluminense (UFF)
instacron:UFF
instname_str Universidade Federal Fluminense (UFF)
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institution UFF
reponame_str Repositório Institucional da Universidade Federal Fluminense (RIUFF)
collection Repositório Institucional da Universidade Federal Fluminense (RIUFF)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)
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