Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
Texto Completo: | https://app.uff.br/riuff/handle/1/17631 |
Resumo: | The aim of this work is to evaluate the presence of Charcot-Leyden crystals in stool specimens as an auxiliary reliable laboratorial diagnostic method and to establish a methodological protocol of parasitological exam of feces to determine the presence of enteroparasitosis. Stool and blood samples of two distinct populations were analyzed in order to determine the prevalence. The first group was composed of 168 children (of both sexes and between 2 and 5 years and 11 months old) who are enrolled in public daycare centers in Niterói, in the state of Rio de Janeiro. The second group was composed of 369 patients from the Antônio Pedro University hospital (HUAP). All exams from both groups were analyzed in the same period, in the Hematology and Parasitology Laboratories of HUAP. The stool specimens from the children were collected in labeled plastic vials with 10% buffered formaldehyde and processed through the standard Coprotest method. Specific white cell count was determined from the blood that was collected by finger prick with appropriate lancet. The blood smear was prepared immediately, stained with Wright stain and subsequently analyzed under a optic microscope with a 1000 fold magnification. The counts were undertaken with the help of a differential cell counter. The blood samples of the outpatients from HUAP were processed in the hospitals Hematology Laboratory, in an automatic cell counter. The parasitological exams of the stool specimens of both outpatients and children were processed by routine methods used in HUAP s Parasitology Laboratory. In short, smears were prepared as of the stool samples and stained subsequently with Wright stain to search for the presence of Charcot-Leyden Crystals. The stool smears were analyzed under an optic microscope with a 1000 fold increase to determine the presence of enteroparasitosis and Charcot-Leyden Crystals. The presence of eosinophils in the blood smears was searched to establish a possible correlation between the findings in the studied populations. The most frequent intestinal parasites encountered in the daycare center population were: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lambia (8%) while in HUAP´s outpatient population were: Entamoeba coli (15%), Giardia lambia (11%), Strongyloides stercoralis (6%) and Blastocystis hominis (5%). The presence of Charcot-Leyden Crystals showed to be a quick, low cost and highly efficient method in the aid of parasitological exam in stool specimens, when associated to the presence of eosinophilia in the peripheral blood. The detection of these pathogenic enteroparasites suggests poor hygiene conditions of the studied populations |
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Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinaisCristais de Charcot-LeydenEosinofiliaEnteroparasitosesEnteropatia parasitáriaTécnica e procedimento de laboratórioFezesParasitologiaPatologia ClínicaMedicinaCharcot-Leyden CrystalsEosinophilia: EnteroparasitosisCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICAThe aim of this work is to evaluate the presence of Charcot-Leyden crystals in stool specimens as an auxiliary reliable laboratorial diagnostic method and to establish a methodological protocol of parasitological exam of feces to determine the presence of enteroparasitosis. Stool and blood samples of two distinct populations were analyzed in order to determine the prevalence. The first group was composed of 168 children (of both sexes and between 2 and 5 years and 11 months old) who are enrolled in public daycare centers in Niterói, in the state of Rio de Janeiro. The second group was composed of 369 patients from the Antônio Pedro University hospital (HUAP). All exams from both groups were analyzed in the same period, in the Hematology and Parasitology Laboratories of HUAP. The stool specimens from the children were collected in labeled plastic vials with 10% buffered formaldehyde and processed through the standard Coprotest method. Specific white cell count was determined from the blood that was collected by finger prick with appropriate lancet. The blood smear was prepared immediately, stained with Wright stain and subsequently analyzed under a optic microscope with a 1000 fold magnification. The counts were undertaken with the help of a differential cell counter. The blood samples of the outpatients from HUAP were processed in the hospitals Hematology Laboratory, in an automatic cell counter. The parasitological exams of the stool specimens of both outpatients and children were processed by routine methods used in HUAP s Parasitology Laboratory. In short, smears were prepared as of the stool samples and stained subsequently with Wright stain to search for the presence of Charcot-Leyden Crystals. The stool smears were analyzed under an optic microscope with a 1000 fold increase to determine the presence of enteroparasitosis and Charcot-Leyden Crystals. The presence of eosinophils in the blood smears was searched to establish a possible correlation between the findings in the studied populations. The most frequent intestinal parasites encountered in the daycare center population were: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lambia (8%) while in HUAP´s outpatient population were: Entamoeba coli (15%), Giardia lambia (11%), Strongyloides stercoralis (6%) and Blastocystis hominis (5%). The presence of Charcot-Leyden Crystals showed to be a quick, low cost and highly efficient method in the aid of parasitological exam in stool specimens, when associated to the presence of eosinophilia in the peripheral blood. The detection of these pathogenic enteroparasites suggests poor hygiene conditions of the studied populationsO presente trabalho tem como objetivo avaliar a pesquisa de Cristais de Charcort-Leyden nas fezes, como método de diagnóstico laboratorial auxiliar de enteroparasitoses e estabelecer um protocolo metodológico de exame parasitológico de fezes para o diagnóstico das enteroparasitoses, com alta confiabilidade. Foi feito um estudo de prevalência em duas populações distintas. Foram analisadas amostras fecais e sangüíneas de 168 crianças de ambos os sexos, com faixa etária de 2 anos até 5 anos e 11 meses matriculadas em creches municipais e/ou municipalizadas de Niterói-RJ, e de 369 pacientes atendidos no HUAP, que tinham exames a serem analisados na mesma data, nos Laboratórios de Hematologia e Parasitologia do HUAP. Nas creches estudadas as fezes foram colhidas em frascos contendo formol tamponado 10% e processadas pelo método padronizado Coprotest. A contagem da série branca específica foi determinada a partir do sangue colhido de picada de dedo com lanceta apropriada. Foram preparados esfregaços sangüíneos posteriormente corados pelo Wright. Os esfregaços foram analisados ao microscópio óptico com aumento de 1000X e a contagem foi feita com auxílio de contador diferencial de células. As amostras de sangue dos pacientes atendidos no HUAP foram processadas no Laboratório de Hematologia do HUAP, em aparelho de automação. O exame parasitológico de fezes foi realizado no Laboratório de Parasitologia do HUAP. Ambos os exames dos pacientes atendidos no HUAP, foram realizados através de métodos utilizados segundo protocolo dos laboratórios. Com as amostras fecais de ambas populações estudadas, foram feitos esfregaços estirados e corados posteriormente com Wright para pesquisar Cristais de Charcot-Leyden. Esses esfregaços foram analisados em microscópio óptico com aumento 1000X. Foram analisadas a prevalência das enteroparasitoses, presença de Cristais de Charcot-Leyden nas fezes, eosinofilia sangüínea e a correlação destes ítens citados nas populações estudadas. Os parasitos intestinais mais freqüentes nas creches foram: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lamblia (8%). No HUAP foram: Entamoeba coli (15%), Giardia lamblia (11%), Strongyloides stercoralis (6%) e Blastocystis hominis (5%). A detecção desses enteroparasitos patogênicos sugere deficiência na higiene das populações estudadas. Para diagnostica-las, a pesquisa de cristais de Charcot-Leyden nas fezes mostrou ser um método rápido, de baixo custo e boa eficácia para auxiliar o exame parasitológico de fezes, associado à avaliação de eosinofilia sanguíneaPrograma de Pós-graduação em PatologiaPatologiaMacedo, Heloisa Werneck deCPF:99124122734http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707043E6Kang, Hye ChungCPF:00788511122http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4775656D2Mondino, Silvia Susana Bona deCPF:59567546720http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707023A6Reis, Neuzir TrindadeCPF:77800563422Sá, Maria de Fátima Leal Manhães de2021-03-10T20:42:17Z2008-01-112021-03-10T20:42:17Z2007-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/17631porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:42:17Zoai:app.uff.br:1/17631Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T10:47:06.849383Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
title |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
spellingShingle |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais Sá, Maria de Fátima Leal Manhães de Cristais de Charcot-Leyden Eosinofilia Enteroparasitoses Enteropatia parasitária Técnica e procedimento de laboratório Fezes Parasitologia Patologia Clínica Medicina Charcot-Leyden Crystals Eosinophilia: Enteroparasitosis CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
title_short |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
title_full |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
title_fullStr |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
title_full_unstemmed |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
title_sort |
Avaliação da pesquisa de cristais de CHARCOT-LEYDEN nas fezes, como método de diagnóstico laboratorial auxiliar de infecções parasitárias intestinais |
author |
Sá, Maria de Fátima Leal Manhães de |
author_facet |
Sá, Maria de Fátima Leal Manhães de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Macedo, Heloisa Werneck de CPF:99124122734 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707043E6 Kang, Hye Chung CPF:00788511122 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4775656D2 Mondino, Silvia Susana Bona de CPF:59567546720 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707023A6 Reis, Neuzir Trindade CPF:77800563422 |
dc.contributor.author.fl_str_mv |
Sá, Maria de Fátima Leal Manhães de |
dc.subject.por.fl_str_mv |
Cristais de Charcot-Leyden Eosinofilia Enteroparasitoses Enteropatia parasitária Técnica e procedimento de laboratório Fezes Parasitologia Patologia Clínica Medicina Charcot-Leyden Crystals Eosinophilia: Enteroparasitosis CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
topic |
Cristais de Charcot-Leyden Eosinofilia Enteroparasitoses Enteropatia parasitária Técnica e procedimento de laboratório Fezes Parasitologia Patologia Clínica Medicina Charcot-Leyden Crystals Eosinophilia: Enteroparasitosis CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
description |
The aim of this work is to evaluate the presence of Charcot-Leyden crystals in stool specimens as an auxiliary reliable laboratorial diagnostic method and to establish a methodological protocol of parasitological exam of feces to determine the presence of enteroparasitosis. Stool and blood samples of two distinct populations were analyzed in order to determine the prevalence. The first group was composed of 168 children (of both sexes and between 2 and 5 years and 11 months old) who are enrolled in public daycare centers in Niterói, in the state of Rio de Janeiro. The second group was composed of 369 patients from the Antônio Pedro University hospital (HUAP). All exams from both groups were analyzed in the same period, in the Hematology and Parasitology Laboratories of HUAP. The stool specimens from the children were collected in labeled plastic vials with 10% buffered formaldehyde and processed through the standard Coprotest method. Specific white cell count was determined from the blood that was collected by finger prick with appropriate lancet. The blood smear was prepared immediately, stained with Wright stain and subsequently analyzed under a optic microscope with a 1000 fold magnification. The counts were undertaken with the help of a differential cell counter. The blood samples of the outpatients from HUAP were processed in the hospitals Hematology Laboratory, in an automatic cell counter. The parasitological exams of the stool specimens of both outpatients and children were processed by routine methods used in HUAP s Parasitology Laboratory. In short, smears were prepared as of the stool samples and stained subsequently with Wright stain to search for the presence of Charcot-Leyden Crystals. The stool smears were analyzed under an optic microscope with a 1000 fold increase to determine the presence of enteroparasitosis and Charcot-Leyden Crystals. The presence of eosinophils in the blood smears was searched to establish a possible correlation between the findings in the studied populations. The most frequent intestinal parasites encountered in the daycare center population were: Blastocystis hominis (20%), Entamoeba coli (10%), Giardia lambia (8%) while in HUAP´s outpatient population were: Entamoeba coli (15%), Giardia lambia (11%), Strongyloides stercoralis (6%) and Blastocystis hominis (5%). The presence of Charcot-Leyden Crystals showed to be a quick, low cost and highly efficient method in the aid of parasitological exam in stool specimens, when associated to the presence of eosinophilia in the peripheral blood. The detection of these pathogenic enteroparasites suggests poor hygiene conditions of the studied populations |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-01 2008-01-11 2021-03-10T20:42:17Z 2021-03-10T20:42:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/17631 |
url |
https://app.uff.br/riuff/handle/1/17631 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
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Universidade Federal Fluminense (UFF) |
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UFF |
institution |
UFF |
reponame_str |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
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riuff@id.uff.br |
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1811823569215160320 |