Influência Materna na Indução da Resposta Alérgica Alimentar

• Autor(a) principal: Campos, Sylvia Maria Nicolau
• Data de Publicação: 2005
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal Fluminense (RIUFF)
• Texto Completo: https://app.uff.br/riuff/handle/1/17562
• topic: Medicina; Patologia experimental; Relação materno-fetal; Inflamação intestinal crônica; Amendoim; Enteropatias; Inflamação; Intestino; Hipersensibilidade alimentar; Medicina experimental; Camundongos; CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA APLICADA

The neonates immune system is less stimulated during the fetal period than in postnatal life. At birth responses to antigens differ from the adults immune response due to the fact that the neonates immune system is still maturing. However the offspring s immunity is influenced by their mothers immune system via the placenta and breast milk. Reports in medical research indicate that the offspring of allergic mothers are more prone to develop allergy than those born to normal mothers. Food allergy is a common disease in children represented by hypersensitivity reactions and as a consequence may lead to the development of inflammatory bowel disease (IBD). Our aims are to evaluate if mother s IBD, caused by specific antigen reexposure during pregnancy, influences the offspring s allergic response and interferes in her survival and/or mortality. Immune and normal C57Bl/6J mice were given a challenge diet of peanuts or mouse chow as of the 2nd week of pregnancy. Half of the offspring received daily 10µl of peanut protein extract by the oral route during the first week of life to induce food allergy. All animals were then imunized with 100µg of peanut protein extract at 2 months of age. One week after sc challenge all were given a diet containing peanuts for 30 days. We evaluated total IgG titers, height and width of villi, number of villi in duodenum area, number of enterocytes (Ent), intraepithelial leukocytes (IEL), the Ent/IEL and Ent/lamina propria (LP) ratio of duodenum segments, as well as goblet cells in villosities and leukocytes in muscular layer. Our results show that the mother´s immunization as well as the diet during pregnancy did not influence the offspring allergic response. However the mortality was significantly higher in the offspring from allergic mothers (p<0.002). The early exposure to low doses to peanut protein did not interfere in the responses to peanuts as of the weaning. There was no significant difference in the IgG titers in the weaning and after each immunization. These differences just exist between immunized and normal offspring, independently of mother´s treatment (p<0.001). The histopathologic analysis confirmed the sorology results and significant differences were observed in these parameters when comparing immunized to normal offspring (p<0.001). We conclude that, in this experimental protocol, the maternal immunization is not decisive in the induction of offspring s food allergy but that IBD rises the probability of complications during the gestational and perinatal periods, as for example the occurrence of abortion (re-absorption in mice), increased mortality and cannibalism of the offspring.