Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
Texto Completo: | https://app.uff.br/riuff/handle/1/17906 |
Resumo: | Oral Squamous Cell Carcinoma (OSCC) usually occurs in patients over 60 years old, with alcohol and tobacco history. Its occurrence in young patients may show distinct molecular mechanisms and may include the participation of other risk factors, such as Human Papillomavirus (HPV) and Epstein-Barr virus (EBV), both with oncogenic role. HPV and EBV may interfere in the p53 pathway, a tumor supressor protein, and telomerase expression, inducing neoplasic cells proliferation. This study evaluated 20 cases of OSCC, which were divided in two groups of ten. The first group, named Group A, was made of 10 cases of patients aged 45 years old or less. The second group, named Group B, was made of 10 cases of patients older than 45 years old. The purpose of this study was to analyze the association among HPV, EBV (LMP1), p53 and telomerase (hTERT) in OSCC of patients from the age group of usual manifestation, compared to the group of younger patients, using immunohistochemical technique. It was also evaluated the difference referring the presence of risk factors, tobacco and alcohol, and the histological grade. In group B, the results showed 90% of hTERT immunopositivity and greater correlation with risk factors, and both results had statistical significance. In group A, there was 40% of immunopositivity for hTERT and alcohol and tobacco use were less common. LMP1 was detected in 30% of the 20 cases and all patients belonged to group A. In this group, there was also a greater number of less differentiated histological grade lesions. The p53 was detected in 60% of all cases, without any difference between the groups. It was also of notice that the hTERT positive neoplasias were equally p53 positive, which suggested a synergism between both regarding neoplasic proliferation induction. HPV positivity was identified only in one case of the more aged group. The results suggest, on one hand, that OSCC in young patients may present a distinct biological behavior, probably more aggressive, less associated with conventional risk factors, such as tobacco and alcohol, and with a participation of other exogenous agents, such as EBV. On the other hand, p53 presence in OSCC of any age group shows its importance in the oral carcinogenesis process. Telomerase expression indicates that neoplasic cells can use this mechanism for its continuous replication, especially in p53 positive and more aged patient s tumors. |
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Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovensCarcinoma de células escamosas oralVírus oncogênicosMarcadores tumoraisNeoplasia bucalCarcinoma de células escamosasMarcador biológico de tumorMedicinaPatologia Buco-dentalCNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICAOral Squamous Cell Carcinoma (OSCC) usually occurs in patients over 60 years old, with alcohol and tobacco history. Its occurrence in young patients may show distinct molecular mechanisms and may include the participation of other risk factors, such as Human Papillomavirus (HPV) and Epstein-Barr virus (EBV), both with oncogenic role. HPV and EBV may interfere in the p53 pathway, a tumor supressor protein, and telomerase expression, inducing neoplasic cells proliferation. This study evaluated 20 cases of OSCC, which were divided in two groups of ten. The first group, named Group A, was made of 10 cases of patients aged 45 years old or less. The second group, named Group B, was made of 10 cases of patients older than 45 years old. The purpose of this study was to analyze the association among HPV, EBV (LMP1), p53 and telomerase (hTERT) in OSCC of patients from the age group of usual manifestation, compared to the group of younger patients, using immunohistochemical technique. It was also evaluated the difference referring the presence of risk factors, tobacco and alcohol, and the histological grade. In group B, the results showed 90% of hTERT immunopositivity and greater correlation with risk factors, and both results had statistical significance. In group A, there was 40% of immunopositivity for hTERT and alcohol and tobacco use were less common. LMP1 was detected in 30% of the 20 cases and all patients belonged to group A. In this group, there was also a greater number of less differentiated histological grade lesions. The p53 was detected in 60% of all cases, without any difference between the groups. It was also of notice that the hTERT positive neoplasias were equally p53 positive, which suggested a synergism between both regarding neoplasic proliferation induction. HPV positivity was identified only in one case of the more aged group. The results suggest, on one hand, that OSCC in young patients may present a distinct biological behavior, probably more aggressive, less associated with conventional risk factors, such as tobacco and alcohol, and with a participation of other exogenous agents, such as EBV. On the other hand, p53 presence in OSCC of any age group shows its importance in the oral carcinogenesis process. Telomerase expression indicates that neoplasic cells can use this mechanism for its continuous replication, especially in p53 positive and more aged patient s tumors.O Carcinoma de Células Escamosas Oral (CCEO) ocorre mais comumente em pacientes acima dos 60 anos de idade, com história de tabagismo e etilismo. A sua ocorrência em indivíduos jovens pode apresentar mecanismos moleculares distintos e a participação de outros fatores de risco, como o Papilomavírus Humano (HPV) e o vírus Epstein-Barr (EBV), ambos com ação oncogênica. O HPV e o EBV podem interferir na ação da p53, uma proteína supressora tumoral, bem como na expressão da telomerase, induzindo à proliferação das células neoplásicas. Este estudo avaliou 20 casos de CCEO, divididos em dois grupos de 10. O primeiro grupo, ou Grupo A, composto de 10 casos de indivíduos de idade até 45 anos, e segundo grupo, ou Grupo B, de 10 casos de pacientes maiores de 45 anos. O objetivo foi analisar, através da técnica da imuno-histoquímica, a associação entre o HPV, o EBV, a p53 e a Telomerase (hTERT) em CCEO de pacientes da faixa etária habitual de manifestação, comparados ao grupo de pacientes mais jovens. Também foram analisadas as diferenças referentes à presença dos fatores de risco, tabaco e álcool e o grau de diferenciação histológica do tumor. Os resultados mostraram que, no grupo B, houve 90% de casos hTERT positivos e maior correlação com os fatores de risco analisados, sendo ambos os resultados estatisticamente significativos. No grupo A, os casos hTERT positivos foram 40% e uma história de tabagismo e de etilismo foi relatada, menos freqüentemente. A LMP1 foi detectada em 30% dos 20 casos e todos os pacientes pertenciam ao grupo A. Nesse grupo, também houve maior número de casos de lesões morfologicamente menos diferenciadas. A p53 foi detectada em 60% do total de casos, sem haver diferenças de expressão entre os grupos. Também foi verificado que as neoplasias hTERT positivas eram igualmente p53 positivas, sugerindo uma ação sinérgica entre ambos, nos mecanismos de indução da proliferação neoplásica. A positividade para o HPV foi identificada em apenas um caso do grupo dos pacientes de maior faixa etária. Os resultados sugerem que o CCEO de pacientes jovens pode apresentar um comportamento biológico distinto, possivelmente mais agressivo, menos associado aos fatores de risco convencionais, como o tabaco e o álcool e com uma participação de outros agentes exógenos, como o EBV. Por outro lado, a presença da p53 em CCEO de qualquer faixa etária, mostra a sua importância no processo da carcinogênse oral. A expressão da telomerase, por sua vez, indica que as células neoplásicas podem dispor desse mecanismo para a sua contínua replicação, principalmente em tumores p53 positivos e provenientes de pacientes de idade mais avançada.Programa de Pós-graduação em PatologiaPatologiaCabral, Márcia GrilloCPF:88322017715http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704341Y5&dataRevisao=nullFonseca, Eliene Carvalho daCPF:41155367522http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782044E4Cardoso, Abel SilveiraCPF:00290874734Nunes, Fabio DaumasCPF:71712512022http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703945Y8Lourenço, Simone de Queiroz ChavesCPF:01456525727http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795619T7Venturi, Beatriz da Rocha Miranda2021-03-10T20:43:04Z2008-06-192021-03-10T20:43:04Z2004-02-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/17906porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:43:04Zoai:app.uff.br:1/17906Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202024-08-19T10:47:11.681360Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
title |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
spellingShingle |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens Venturi, Beatriz da Rocha Miranda Carcinoma de células escamosas oral Vírus oncogênicos Marcadores tumorais Neoplasia bucal Carcinoma de células escamosas Marcador biológico de tumor Medicina Patologia Buco-dental CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
title_short |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
title_full |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
title_fullStr |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
title_full_unstemmed |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
title_sort |
Estudo comparativo da correlação entre HPV, EBV, p53 e Telomerase em carcinoma de células escamosas oral em adultos e adultos jovens |
author |
Venturi, Beatriz da Rocha Miranda |
author_facet |
Venturi, Beatriz da Rocha Miranda |
author_role |
author |
dc.contributor.none.fl_str_mv |
Cabral, Márcia Grillo CPF:88322017715 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704341Y5&dataRevisao=null Fonseca, Eliene Carvalho da CPF:41155367522 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782044E4 Cardoso, Abel Silveira CPF:00290874734 Nunes, Fabio Daumas CPF:71712512022 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703945Y8 Lourenço, Simone de Queiroz Chaves CPF:01456525727 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795619T7 |
dc.contributor.author.fl_str_mv |
Venturi, Beatriz da Rocha Miranda |
dc.subject.por.fl_str_mv |
Carcinoma de células escamosas oral Vírus oncogênicos Marcadores tumorais Neoplasia bucal Carcinoma de células escamosas Marcador biológico de tumor Medicina Patologia Buco-dental CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
topic |
Carcinoma de células escamosas oral Vírus oncogênicos Marcadores tumorais Neoplasia bucal Carcinoma de células escamosas Marcador biológico de tumor Medicina Patologia Buco-dental CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA |
description |
Oral Squamous Cell Carcinoma (OSCC) usually occurs in patients over 60 years old, with alcohol and tobacco history. Its occurrence in young patients may show distinct molecular mechanisms and may include the participation of other risk factors, such as Human Papillomavirus (HPV) and Epstein-Barr virus (EBV), both with oncogenic role. HPV and EBV may interfere in the p53 pathway, a tumor supressor protein, and telomerase expression, inducing neoplasic cells proliferation. This study evaluated 20 cases of OSCC, which were divided in two groups of ten. The first group, named Group A, was made of 10 cases of patients aged 45 years old or less. The second group, named Group B, was made of 10 cases of patients older than 45 years old. The purpose of this study was to analyze the association among HPV, EBV (LMP1), p53 and telomerase (hTERT) in OSCC of patients from the age group of usual manifestation, compared to the group of younger patients, using immunohistochemical technique. It was also evaluated the difference referring the presence of risk factors, tobacco and alcohol, and the histological grade. In group B, the results showed 90% of hTERT immunopositivity and greater correlation with risk factors, and both results had statistical significance. In group A, there was 40% of immunopositivity for hTERT and alcohol and tobacco use were less common. LMP1 was detected in 30% of the 20 cases and all patients belonged to group A. In this group, there was also a greater number of less differentiated histological grade lesions. The p53 was detected in 60% of all cases, without any difference between the groups. It was also of notice that the hTERT positive neoplasias were equally p53 positive, which suggested a synergism between both regarding neoplasic proliferation induction. HPV positivity was identified only in one case of the more aged group. The results suggest, on one hand, that OSCC in young patients may present a distinct biological behavior, probably more aggressive, less associated with conventional risk factors, such as tobacco and alcohol, and with a participation of other exogenous agents, such as EBV. On the other hand, p53 presence in OSCC of any age group shows its importance in the oral carcinogenesis process. Telomerase expression indicates that neoplasic cells can use this mechanism for its continuous replication, especially in p53 positive and more aged patient s tumors. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-02-13 2008-06-19 2021-03-10T20:43:04Z 2021-03-10T20:43:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/17906 |
url |
https://app.uff.br/riuff/handle/1/17906 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Patologia Patologia |
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reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
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UFF |
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UFF |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
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Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
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riuff@id.uff.br |
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