Expressão gênica diferencial de quatro espécies da Aliança Tabebuia em resposta ao deficit hídrico

• Autor(a) principal: Sobreiro, Mariane Brom
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000087v2
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/7212
• topic: Estresse hídrico; Handroanthus impetiginosus; Handroanthus ochraceus; Handroanthus serratifolius; Ipê; RNA-seq; Tabebuia aurea; Drought stress; Handroanthus ochraceus; Handroanthus impetiginosus; Handroanthus serratifolius; Ipe; RNA-seq; Tabebuia aurea; CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Considering the rate of increase in average annual temperature and the seasonality of rainfall in several regions of the country, investigations on the mechanisms of plant’s response to low water availability become relevant. Tabebuia Alliance species - monophyletic clade of the Bignoniaceae family are commonly known as ipe – are distribuited in areas with different soil and climatic conditions. This feature makes them an interesting model to understand mechanisms tolerance’s to abiotic stresses. For each species there were two groups: control group, which had maintained irrigation; low water availability group, which irrigation was stopped and the experiment continued until the subtrate reached 40% of field capacity. The main objective of this work was to identify differentially expressed genes (DEG) in four species - two from the Brazilian savannah (T. aurea and Handroanthus ochraceus) and two from seasonally dry forests (H. impetiginosus and H. serratifolius). Then, RNA was extracted from the plants for sequencing on the Illumina platform with paired-end sequences of 100 base pairs (bp). Sequences were evaluated for quality control and mapped onto the genome of H. impetiginosus to identify DEG using the R software. The DGEs obtained by DESeq2 were subjected to functional enrichment analysis and potential changes in the level of expression of genes encoding enzymes of particular metabolic pathways. In all tools, H. serratifolius species showed the highest number of DGE (4908 noDESeq2), while H. ochraceus had the lowest number of DGE (6 in DESeq2). Functional enrichment analyzes demonstrated that the species presented, individually or collectively, typical responses of low water availability such as decrease of photosynthetic rate, increase of proline and increase of starch degradation. Although species share some responses, the complexity of organisms does not allow them to exhibit identical behaviors.