Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/4835 |
Resumo: | The actinobacteria constitute a wide and diverse phylum of gram-positive bacteria with a high content of guanine and cytosine in their DNA, standing out by the production of secondary metabolites with antibiotic activity. The treatment of multi-drug resistant pathogens infections and cancer represents a major challenge for modern medicine and bioactive microbial metabolites constitute a rich source of antimicrobial and antitumor drugs. The main aim of this study was to analyse the potential antimicrobial and cytotoxic activities of actinobactéria isolated from various habitats. The evaluation of these bioactivities was accomplished by the application of a set of qualitative screening in vitro assays. From a total of 19 morphospecies, isolated from the goiano Cerrado soil, 16 were evaluated in the primary antimicrobial activity tests, of which 5 (31,25%) antagonized the growth of indicator microorganisms. The ECL of the morphospecie BC-A22 and the crude extracts of the strain ADU 1.3 stood out for their strong antibiotic action against MRSA. The most of the crude extracts exhibited lytic action on the staphylococcal cell wall. Only ECLs of morphospecies GUARA 1 and PEG 23 and extracts of the strain PEG 30 were cytotoxic against A. salina in low concentration. None of the extracts showed an intercalation effect on the DNA molecule. With exception of BC-A22, the other six isolates were morphologically characterized as members of the Streptomyces genus. The morphospecie ADU 1.3 was molecularly identified as Streptomyces sp., highly likely to consist of a new species. The results of this initial phase of microbial bioprospecting highlight that all the isolates are potential producers of antibiotics biomolecules, excelling the morphospecie BC-A22, which exhibited a strong antimicrobial activity against the clinical isolates of MRSA and most of gram-negative bacteria. |
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Vieira, José Daniel Gonçalveshttp://lattes.cnpq.br/1742731776579730Vieira, José Daniel GonçalvesUlhoa, Cirano JoséSouza, Keili Maria Cardoso dehttp://lattes.cnpq.br/0409870707537485Oliveira, Bruno Francesco Rodrigues de2015-10-29T14:47:16Z2015-03-18OLIVEIRA, B. F. R. Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats. 2015. 151 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/4835The actinobacteria constitute a wide and diverse phylum of gram-positive bacteria with a high content of guanine and cytosine in their DNA, standing out by the production of secondary metabolites with antibiotic activity. The treatment of multi-drug resistant pathogens infections and cancer represents a major challenge for modern medicine and bioactive microbial metabolites constitute a rich source of antimicrobial and antitumor drugs. The main aim of this study was to analyse the potential antimicrobial and cytotoxic activities of actinobactéria isolated from various habitats. The evaluation of these bioactivities was accomplished by the application of a set of qualitative screening in vitro assays. From a total of 19 morphospecies, isolated from the goiano Cerrado soil, 16 were evaluated in the primary antimicrobial activity tests, of which 5 (31,25%) antagonized the growth of indicator microorganisms. The ECL of the morphospecie BC-A22 and the crude extracts of the strain ADU 1.3 stood out for their strong antibiotic action against MRSA. The most of the crude extracts exhibited lytic action on the staphylococcal cell wall. Only ECLs of morphospecies GUARA 1 and PEG 23 and extracts of the strain PEG 30 were cytotoxic against A. salina in low concentration. None of the extracts showed an intercalation effect on the DNA molecule. With exception of BC-A22, the other six isolates were morphologically characterized as members of the Streptomyces genus. The morphospecie ADU 1.3 was molecularly identified as Streptomyces sp., highly likely to consist of a new species. The results of this initial phase of microbial bioprospecting highlight that all the isolates are potential producers of antibiotics biomolecules, excelling the morphospecie BC-A22, which exhibited a strong antimicrobial activity against the clinical isolates of MRSA and most of gram-negative bacteria.As actinobactérias compõem um amplo e diverso filo de bactérias gram-positivas com elevado conteúdo de guanina e citosina em seu DNA, destacando-se pela produção de metabólitos secundários com atividade antibiótica. O tratamento das infecções por patógenos multirresistentes aos antimicrobianos e do câncer representa um dos maiores desafios da medicina moderna e moléculas microbianas bioativas ainda constituem uma fonte rica de drogas antimicrobianas e antitumorais. O objetivo principal do presente estudo foi analisar as potenciais ações antimicrobiana e citotóxica de actinobactérias isoladas de vários habitats. A avaliação dessas bioatividades foi efetuada mediante aplicação de uma série de ensaios qualitativos de triagem in vitro. Do total de 19 morfoespécies, isoladas do solo de Cerrado goiano, 16 foram avaliadas nos testes primários de atividade antimicrobiana, das quais 5 (31,25%) antagonizaram o crescimento dos micro-organismos indicadores. O ECL da morfoespécie BC-A22 e os extratos brutos do isolado ADU 1.3 se sobressaíram quanto a sua alta ação antibiótica frente à MRSA. A maioria dos extratos brutos exibiu ação lítica sobre a parede celular estafilocóccica. Apenas os ECLs das morfoespécies GUARA 1 e PEG 23 e os extratos do isolado PEG 30 foram citotóxicos a A. salina em baixa concentração. Nenhum dos extratos mostrou efeito de intercalação sob a molécula de DNA. Com exceção de BC-A22, os seis isolados foram caracterizados morfologicamente como membros do gênero Streptomyces. A morfoespécie ADU 1.3 foi identificada molecularmente como Streptomyces sp., com alta probabilidade de consistir em uma espécie nova. Os resultados obtidos dessa etapa de bioprospecção inicial evidenciam que todos os sete isolados são potenciais produtores de biomoléculas antibióticas, notabilizando-se a morfoespécie BC-A22, que exibiu uma alta ação antimicrobiana frente aos MRSA hospitalares e a maior parte das bactérias gram-negativas. Palavras-chave: actinobactérias; bioprospecção microbiana; metabólitos bioativos; antibióticos.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-10-29T14:44:05Z No. of bitstreams: 2 Dissertação - Bruno Francesco Rodrigues de Oliveira - 2015.pdf: 3313694 bytes, checksum: 5cb9779c379ed9f47de7c0d65abeb95b (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-10-29T14:47:16Z (GMT) No. of bitstreams: 2 Dissertação - Bruno Francesco Rodrigues de Oliveira - 2015.pdf: 3313694 bytes, checksum: 5cb9779c379ed9f47de7c0d65abeb95b (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2015-10-29T14:47:16Z (GMT). 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dc.title.por.fl_str_mv |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
dc.title.alternative.eng.fl_str_mv |
Analyses of antimicrobial and cytotoxic activities of actinobacteria isolated from diverse habitats |
title |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
spellingShingle |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats Oliveira, Bruno Francesco Rodrigues de Actinobactérias Bioprospecção microbiana Metabólitos bioativos Antibióticos Actinobacteria Microbial bioprospecting Bioactive metabolites Antibiotics CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
title_full |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
title_fullStr |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
title_full_unstemmed |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
title_sort |
Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats |
author |
Oliveira, Bruno Francesco Rodrigues de |
author_facet |
Oliveira, Bruno Francesco Rodrigues de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vieira, José Daniel Gonçalves |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1742731776579730 |
dc.contributor.referee1.fl_str_mv |
Vieira, José Daniel Gonçalves |
dc.contributor.referee2.fl_str_mv |
Ulhoa, Cirano José |
dc.contributor.referee3.fl_str_mv |
Souza, Keili Maria Cardoso de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0409870707537485 |
dc.contributor.author.fl_str_mv |
Oliveira, Bruno Francesco Rodrigues de |
contributor_str_mv |
Vieira, José Daniel Gonçalves Vieira, José Daniel Gonçalves Ulhoa, Cirano José Souza, Keili Maria Cardoso de |
dc.subject.por.fl_str_mv |
Actinobactérias Bioprospecção microbiana Metabólitos bioativos Antibióticos |
topic |
Actinobactérias Bioprospecção microbiana Metabólitos bioativos Antibióticos Actinobacteria Microbial bioprospecting Bioactive metabolites Antibiotics CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
Actinobacteria Microbial bioprospecting Bioactive metabolites Antibiotics |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
The actinobacteria constitute a wide and diverse phylum of gram-positive bacteria with a high content of guanine and cytosine in their DNA, standing out by the production of secondary metabolites with antibiotic activity. The treatment of multi-drug resistant pathogens infections and cancer represents a major challenge for modern medicine and bioactive microbial metabolites constitute a rich source of antimicrobial and antitumor drugs. The main aim of this study was to analyse the potential antimicrobial and cytotoxic activities of actinobactéria isolated from various habitats. The evaluation of these bioactivities was accomplished by the application of a set of qualitative screening in vitro assays. From a total of 19 morphospecies, isolated from the goiano Cerrado soil, 16 were evaluated in the primary antimicrobial activity tests, of which 5 (31,25%) antagonized the growth of indicator microorganisms. The ECL of the morphospecie BC-A22 and the crude extracts of the strain ADU 1.3 stood out for their strong antibiotic action against MRSA. The most of the crude extracts exhibited lytic action on the staphylococcal cell wall. Only ECLs of morphospecies GUARA 1 and PEG 23 and extracts of the strain PEG 30 were cytotoxic against A. salina in low concentration. None of the extracts showed an intercalation effect on the DNA molecule. With exception of BC-A22, the other six isolates were morphologically characterized as members of the Streptomyces genus. The morphospecie ADU 1.3 was molecularly identified as Streptomyces sp., highly likely to consist of a new species. The results of this initial phase of microbial bioprospecting highlight that all the isolates are potential producers of antibiotics biomolecules, excelling the morphospecie BC-A22, which exhibited a strong antimicrobial activity against the clinical isolates of MRSA and most of gram-negative bacteria. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-10-29T14:47:16Z |
dc.date.issued.fl_str_mv |
2015-03-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, B. F. R. Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats. 2015. 151 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/4835 |
identifier_str_mv |
OLIVEIRA, B. F. R. Análise das atividades antimicrobiana e citotóxica de actinobactérias isoladas de diversos habitats. 2015. 151 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/4835 |
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por |
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por |
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dc.relation.cnpq.fl_str_mv |
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dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
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Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia das Interações PH (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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bitstream.checksum.fl_str_mv |
5cb9779c379ed9f47de7c0d65abeb95b bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f 683d9883b2ad62ac3b8bafc566b2e600 9da0b6dfac957114c6a7714714b86306 977a76820854f21d65ce0df8a3f26941 f3b6477618fc57e31b016f9fe3f55be5 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044387298508800 |