Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional

Detalhes bibliográficos
Autor(a) principal: Santos, Adailton Pereira dos
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8698
Resumo: β-lactamases are enzymes that hydrolyze the β-lactam ring, inactivating the action of β-lactam antibiotics. The objective of this work was to diagnose phenotypically and molecularly 14 β- lactamase resistance genes expressed in Pseudomonas aeruginosa and to correlate the results found. A total of 99 samples of Pseudomonas aeruginosa were selected and the antibiogram was performed. Real-time PCR is being performed using the Sybr Green system to amplify the genes corresponding to the resistances found in phenotyping. Three/14 (21.4%) genes blaSME, blaOXA, blaGIM were found simultaneously in three samples. According to the statistical test, when evaluating the amplification results obtained for PPT, the molecular method was more sensitive for the detection of the gene coding for multidrug resistance, presenting values of (p <0.05). This information suggests that gene research is more sensitive and specific compared to the antibiogram.
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spelling Carneiro, Lilian Carlahttp://lattes.cnpq.br/6506744224041777Braga, Carla Afonso da Silva Bitencourthttp://lattes.cnpq.br/7673897995590123Carneiro, Lilian CarlaAmaral, André CorrêaBarbosa, Mônica SantiagoSantos, Mônica de OliveiraVieira Filho, Aroldo Moraeshttp://lattes.cnpq.br/2927204642170936Santos, Adailton Pereira dos2018-07-17T13:12:23Z2018-06-29SANTOS, A. P. Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional. 2018. 114 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/8698ark:/38995/0013000000d02β-lactamases are enzymes that hydrolyze the β-lactam ring, inactivating the action of β-lactam antibiotics. The objective of this work was to diagnose phenotypically and molecularly 14 β- lactamase resistance genes expressed in Pseudomonas aeruginosa and to correlate the results found. A total of 99 samples of Pseudomonas aeruginosa were selected and the antibiogram was performed. Real-time PCR is being performed using the Sybr Green system to amplify the genes corresponding to the resistances found in phenotyping. Three/14 (21.4%) genes blaSME, blaOXA, blaGIM were found simultaneously in three samples. According to the statistical test, when evaluating the amplification results obtained for PPT, the molecular method was more sensitive for the detection of the gene coding for multidrug resistance, presenting values of (p <0.05). This information suggests that gene research is more sensitive and specific compared to the antibiogram.As β-lactamases são enzimas que hidrolisam o anel β-lactâmico, inativando a ação de antibióticos β-lactâmicos. O objetivo deste trabalho foi diagnosticar fenotipicamente e molecularmente 14 genes de resistência à β-lactamases expressos em Pseudomonas aeruginosa e correlacionar os resultados encontrados. Um total de 99 amostras de Pseudomonas aeruginosa foi selecionado e o antibiograma foi realizado. A PCR em tempo real foi realizada usando o sistema Sybr Green para amplificar os genes correspondentes às resistências encontradas na fenotipagem. Das 99 amostras, 14 foram identificadas como fenotipicamente resistentes ao ATM antimicrobiano. Três/14 (21,4%) genes blaSME, blaOXA, blaGIM foram encontrados simultaneamente em três amostras. De acordo com o teste estatístico, ao avaliar os resultados de amplificação obtidos para o PPT, o método molecular foi ma s sensível para a detecção do gene que codifica a resistência a múltiplos fármacos, apresentando valores de (p <0,05). Esta informação sugere que a pesquisa genética é mais sensível e específica em comparação com o antibiograma.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2018-07-16T11:25:09Z No. of bitstreams: 2 Dissertação - Adailton Pereira dos Santos - 2018.pdf: 2470326 bytes, checksum: b19858377cb05cc2abd767e4560cd12a (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Cláudia Bueno (claudiamoura18@gmail.com) on 2018-07-17T13:12:23Z (GMT) No. of bitstreams: 2 Dissertação - Adailton Pereira dos Santos - 2018.pdf: 2470326 bytes, checksum: b19858377cb05cc2abd767e4560cd12a (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-07-17T13:12:23Z (GMT). No. of bitstreams: 2 Dissertação - Adailton Pereira dos Santos - 2018.pdf: 2470326 bytes, checksum: b19858377cb05cc2abd767e4560cd12a (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-06-29application/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências da Saúde (FM)UFGBrasilFaculdade de Medicina - FM (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAntibiogramaResistência antimicrobianaβ- lactâmicosGenesDiagnóstico molecularAntibiogramAntimicrobial resistanceβ-lactamsGenesMolecular diagnosisCIENCIAS DA SAUDEPerfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacionalResearch training profile of Pseudomonas aeruginosa and follow-up of the educational routineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-100686431261774531060060060015457724759504863388765449414823306929reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Adailton Pereira dos Santos - 2018.pdfDissertação - Adailton Pereira dos Santos - 2018.pdfapplication/pdf2470326http://repositorio.bc.ufg.br/tede/bitstreams/f7004d6f-2466-436a-82e9-71c19cd2f4cc/downloadb19858377cb05cc2abd767e4560cd12aMD55LICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
dc.title.alternative.eng.fl_str_mv Research training profile of Pseudomonas aeruginosa and follow-up of the educational routine
title Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
spellingShingle Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
Santos, Adailton Pereira dos
Antibiograma
Resistência antimicrobiana
β- lactâmicos
Genes
Diagnóstico molecular
Antibiogram
Antimicrobial resistance
β-lactams
Genes
Molecular diagnosis
CIENCIAS DA SAUDE
title_short Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
title_full Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
title_fullStr Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
title_full_unstemmed Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
title_sort Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
author Santos, Adailton Pereira dos
author_facet Santos, Adailton Pereira dos
author_role author
dc.contributor.advisor1.fl_str_mv Carneiro, Lilian Carla
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6506744224041777
dc.contributor.advisor-co1.fl_str_mv Braga, Carla Afonso da Silva Bitencourt
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7673897995590123
dc.contributor.referee1.fl_str_mv Carneiro, Lilian Carla
dc.contributor.referee2.fl_str_mv Amaral, André Corrêa
dc.contributor.referee3.fl_str_mv Barbosa, Mônica Santiago
dc.contributor.referee4.fl_str_mv Santos, Mônica de Oliveira
dc.contributor.referee5.fl_str_mv Vieira Filho, Aroldo Moraes
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2927204642170936
dc.contributor.author.fl_str_mv Santos, Adailton Pereira dos
contributor_str_mv Carneiro, Lilian Carla
Braga, Carla Afonso da Silva Bitencourt
Carneiro, Lilian Carla
Amaral, André Corrêa
Barbosa, Mônica Santiago
Santos, Mônica de Oliveira
Vieira Filho, Aroldo Moraes
dc.subject.por.fl_str_mv Antibiograma
Resistência antimicrobiana
β- lactâmicos
Genes
Diagnóstico molecular
topic Antibiograma
Resistência antimicrobiana
β- lactâmicos
Genes
Diagnóstico molecular
Antibiogram
Antimicrobial resistance
β-lactams
Genes
Molecular diagnosis
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv Antibiogram
Antimicrobial resistance
β-lactams
Genes
Molecular diagnosis
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description β-lactamases are enzymes that hydrolyze the β-lactam ring, inactivating the action of β-lactam antibiotics. The objective of this work was to diagnose phenotypically and molecularly 14 β- lactamase resistance genes expressed in Pseudomonas aeruginosa and to correlate the results found. A total of 99 samples of Pseudomonas aeruginosa were selected and the antibiogram was performed. Real-time PCR is being performed using the Sybr Green system to amplify the genes corresponding to the resistances found in phenotyping. Three/14 (21.4%) genes blaSME, blaOXA, blaGIM were found simultaneously in three samples. According to the statistical test, when evaluating the amplification results obtained for PPT, the molecular method was more sensitive for the detection of the gene coding for multidrug resistance, presenting values of (p <0.05). This information suggests that gene research is more sensitive and specific compared to the antibiogram.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-07-17T13:12:23Z
dc.date.issued.fl_str_mv 2018-06-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv SANTOS, A. P. Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional. 2018. 114 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/8698
dc.identifier.dark.fl_str_mv ark:/38995/0013000000d02
identifier_str_mv SANTOS, A. P. Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional. 2018. 114 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018.
ark:/38995/0013000000d02
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
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dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Faculdade de Medicina - FM (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
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