Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/13035 |
Resumo: | Hancornia speciosa Gomes, popularly known as mangabeira, is a native fruit species of the Cerrado used for food purposes and as herbal medicine. Currently, due to habitat loss and predatory extraction, there is a great demand in more studies for the conservation and use of this fruit species. The main challenge for the in vitro establishment of the species is the high number of endophytic agents (fungi and bacteria) that are present in plant tissues. This makes it more difficult to introduce plant material into the in vitro culture. This work aimed to optimize the in vitro establishment protocols in addition to carrying out vegetative propagation, via layering, generating clones of matrix plants. For the in vitro establishment of one of the varieties of the species, var. gardineri, first the fall fruits were collected in the Collection of Native Fruits of the Cerrado of the School of Agronomy at UFG. Fruits were pulped and 80 seeds were selected, which had their tegument removed and proceeded to the decontamination process. In a laminar flow chamber, two groups of seeds were separated: 40 seeds were opened and their embryos exposed, isolated and inoculated in MS culture medium; the other 40 seeds were inoculated directly in MS culture medium. To test the effect of stretching on reducing the period of development in vitro, embryos were inoculated in treatments with 0, 1, 2 and 4 μM of gibberellic acid (GA3) and later in a second experiment in MS medium with 1,0 μM of 6-benzylaminopurine (BAP), with lower concentrations of GA3: 0.2; 0.4; 0.6; 0.8 and 1 μM. For the next stage, treatments were carried out to root the new shoots with indolbutyric acid (IBA) in concentrations 0, 1, 3, 5 and 7 mg.mL-1 . The germination of the isolated embryos showed less than 5% of contamination and was 30% faster when compared to the seed. The effect of GA3 on isolated embryos allowed the plant to elongate with 0.8 μM, reaching 130 mm in 75 days, reducing in vitro cultivation by more than 100 days. In rooting, the concentration of 1 mg.mL-1 of AIB allowed more than 9 roots per plant and an average length of 54 mm. The acclimatization of the treatment plants took place on substrate with 50% sand and 50% broad red soil with commercial substrate. In this process, mortality was less than 10%. The layering experiments were carried out with four varieties of H. speciosa: gardineri, speciosa, cuyabensis and pubescens and in two different seasons: the dry season, and the rainy season. AIB was used to induce rooting at concentrations of 0, 4, 5 and 6 mg.mL-1 , with 15 ml per branch. The substrate used was soil humidified with commercial substrate fixed to the ringed branch. After 180 days, the best results were in the concentration of 5 mg.mL-1 in the four varieties used. However, the results for each variety were at different times of the year, due to the specific phenological phases. |
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Sibov, Sérgio Tadeuhttp://lattes.cnpq.br/4627553641870284Sibov, Sérgio TadeuGanga, Rita Maria DevósAguiar, Renata Alves dehttp://lattes.cnpq.br/9506144829333238Tiago, Bruno dos Santos2023-09-08T11:32:28Z2023-09-08T11:32:28Z2020-02-28TIAGO, B. S. Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes). 2020. 68 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/13035ark:/38995/0013000000gksHancornia speciosa Gomes, popularly known as mangabeira, is a native fruit species of the Cerrado used for food purposes and as herbal medicine. Currently, due to habitat loss and predatory extraction, there is a great demand in more studies for the conservation and use of this fruit species. The main challenge for the in vitro establishment of the species is the high number of endophytic agents (fungi and bacteria) that are present in plant tissues. This makes it more difficult to introduce plant material into the in vitro culture. This work aimed to optimize the in vitro establishment protocols in addition to carrying out vegetative propagation, via layering, generating clones of matrix plants. For the in vitro establishment of one of the varieties of the species, var. gardineri, first the fall fruits were collected in the Collection of Native Fruits of the Cerrado of the School of Agronomy at UFG. Fruits were pulped and 80 seeds were selected, which had their tegument removed and proceeded to the decontamination process. In a laminar flow chamber, two groups of seeds were separated: 40 seeds were opened and their embryos exposed, isolated and inoculated in MS culture medium; the other 40 seeds were inoculated directly in MS culture medium. To test the effect of stretching on reducing the period of development in vitro, embryos were inoculated in treatments with 0, 1, 2 and 4 μM of gibberellic acid (GA3) and later in a second experiment in MS medium with 1,0 μM of 6-benzylaminopurine (BAP), with lower concentrations of GA3: 0.2; 0.4; 0.6; 0.8 and 1 μM. For the next stage, treatments were carried out to root the new shoots with indolbutyric acid (IBA) in concentrations 0, 1, 3, 5 and 7 mg.mL-1 . The germination of the isolated embryos showed less than 5% of contamination and was 30% faster when compared to the seed. The effect of GA3 on isolated embryos allowed the plant to elongate with 0.8 μM, reaching 130 mm in 75 days, reducing in vitro cultivation by more than 100 days. In rooting, the concentration of 1 mg.mL-1 of AIB allowed more than 9 roots per plant and an average length of 54 mm. The acclimatization of the treatment plants took place on substrate with 50% sand and 50% broad red soil with commercial substrate. In this process, mortality was less than 10%. The layering experiments were carried out with four varieties of H. speciosa: gardineri, speciosa, cuyabensis and pubescens and in two different seasons: the dry season, and the rainy season. AIB was used to induce rooting at concentrations of 0, 4, 5 and 6 mg.mL-1 , with 15 ml per branch. The substrate used was soil humidified with commercial substrate fixed to the ringed branch. After 180 days, the best results were in the concentration of 5 mg.mL-1 in the four varieties used. However, the results for each variety were at different times of the year, due to the specific phenological phases.Hancornia speciosa Gomes, popularmente conhecida como mangabeira, é uma espécie frutífera nativa do Cerrado utilizada para fins alimentícios e como fitoterápico. Atualmente, pela perda de habitat e pelo extrativismo predatório, há uma grande demanda em mais estudos para a conservação e uso desta frutífera. O estabelecimento in vitro da espécie apresenta como principal desafio o elevado número de agentes endofíticos (fungos e bactérias) que estão presentes nos tecidos da planta. Isso torna mais difícil a introdução de material vegetal na cultura in vitro. Este trabalho visou otimizar os protocolos de estabelecimento in vitro além de realizar a propagação vegetativa, via alporquia, gerando clones de plantas matrizes. Para o estabelecimento in vitro de uma das variedades da espécie, a var. Gardineri, primeiro coletou-se os frutos de queda, na Coleção de Frutíferas Nativas do Cerrado da Escola de Agronomia da UFG. Os frutos foram despolpados e selecionou-se 80 sementes que tiveram seu tegumento removido e seguiram para o processo de descontaminação. Em câmara de fluxo laminar, dois grupos de sementes foram separados: 40 sementes foram abertas e seus embriões expostos, isolados e inoculados em meio de cultura MS; as outras 40 sementes foram inoculadas diretamente em meio de cultura MS. Para testar o efeito do alongamento na redução do período de desenvolvimento in vitro, embriões foram inoculados em tratamentos com 0, 1, 2 e 4 μM de ácido giberélico (GA3) e posteriormente em um segundo experimento em meio MS com 1,0 μM de 6-benzilaminopurina (BAP), com concentrações menores de GA3: 0,2; 0,4; 0,6; 0,8 e 1 μM. Para a etapa seguinte, realizou-se tratamentos para o enraizamento dos novos brotos com ácido indolbutírico (AIB) nas concentrações 0, 1, 3, 5 e 7 mg.mL-1. A germinação dos embriões isolados apresentou menos de 5% de contaminação e foi 30% mais rápida quando comparada com a semente. O efeito do GA3 nos embriões isolados possibilitou o alongamento da planta com 0,8 μM, atingindo 130 mm em 75 dias, reduzindo em mais de 100 dias o cultivo in vitro. No enraizamento, a concentração de 1 mg.mL-1 de AIB possibilitou mais de nove raízes por planta e comprimento médio de 54 mm. A aclimatização das plantas dos tratamentos aconteceu em substrato com 50% de areia e 50% de lato solo vermelho com substrato comercial. Neste processo a mortalidade foi inferior a 10%. Os experimentos de alporquia foram realizados com quatro variedades de H. speciosa: gardineri, speciosa, cuyabensis e pubescens e em duas épocas distintas: a estação seca, e a estação chuvosa. Utilizou-se AIB para a indução de enraizamento nas concentrações de 4 mg/mL-1, 5 mg.mL-1 e 6 mg.mL-1 e controle com 0 mg.mL-1, sendo aplicado 15 ml por ramo. O substrato usado foi solo humificado com substrato comercial fixado no ramo anelado. Após 180 dias, os melhores resultados foram na concentração de 5 mg.mL-1 nas quatro variedades utilizadas. Porém, os resultados em cada variedade, sofreram variação de acordo comas diferentes estações do ano, devido as fases fenológicas específicas.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2023-09-06T19:17:28Z No. of bitstreams: 2 Dissertação - Bruno dos Santos Tiago - 2020.pdf: 18863839 bytes, checksum: 1c359ae48894ef6594cbc45ffb9ad54b (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2023-09-08T11:32:28Z (GMT) No. of bitstreams: 2 Dissertação - Bruno dos Santos Tiago - 2020.pdf: 18863839 bytes, checksum: 1c359ae48894ef6594cbc45ffb9ad54b (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2023-09-08T11:32:28Z (GMT). No. of bitstreams: 2 Dissertação - Bruno dos Santos Tiago - 2020.pdf: 18863839 bytes, checksum: 1c359ae48894ef6594cbc45ffb9ad54b (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2020-02-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Melhoramento de Plantas (EA)UFGBrasilEscola de Agronomia - EA (RMG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCerradoMangabaPropagaçãoCultura de tecidosPropagationTissue cultureCIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETALPropagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes)Vegetative propagation by alporquia and optimization of in vitro establishment protocol of mangabeira (Hancornia speciosa Gomes)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6050050050050026511reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/3e73e84c-4568-46f4-9278-a8ff1263f679/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/7175a1bc-d71d-46fe-a835-c8010da5122f/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Bruno dos Santos Tiago - 2020.pdfDissertação - Bruno dos Santos Tiago - 2020.pdfapplication/pdf18863839http://repositorio.bc.ufg.br/tede/bitstreams/fb7503d5-6ea3-414a-9d09-8913f7fe0915/download1c359ae48894ef6594cbc45ffb9ad54bMD53tede/130352023-09-08 08:32:28.944http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/13035http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2023-09-08T11:32:28Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
dc.title.alternative.eng.fl_str_mv |
Vegetative propagation by alporquia and optimization of in vitro establishment protocol of mangabeira (Hancornia speciosa Gomes) |
title |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
spellingShingle |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) Tiago, Bruno dos Santos Cerrado Mangaba Propagação Cultura de tecidos Propagation Tissue culture CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL |
title_short |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
title_full |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
title_fullStr |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
title_full_unstemmed |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
title_sort |
Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes) |
author |
Tiago, Bruno dos Santos |
author_facet |
Tiago, Bruno dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Sibov, Sérgio Tadeu |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4627553641870284 |
dc.contributor.referee1.fl_str_mv |
Sibov, Sérgio Tadeu |
dc.contributor.referee2.fl_str_mv |
Ganga, Rita Maria Devós |
dc.contributor.referee3.fl_str_mv |
Aguiar, Renata Alves de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9506144829333238 |
dc.contributor.author.fl_str_mv |
Tiago, Bruno dos Santos |
contributor_str_mv |
Sibov, Sérgio Tadeu Sibov, Sérgio Tadeu Ganga, Rita Maria Devós Aguiar, Renata Alves de |
dc.subject.por.fl_str_mv |
Cerrado Mangaba Propagação Cultura de tecidos |
topic |
Cerrado Mangaba Propagação Cultura de tecidos Propagation Tissue culture CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL |
dc.subject.eng.fl_str_mv |
Propagation Tissue culture |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL |
description |
Hancornia speciosa Gomes, popularly known as mangabeira, is a native fruit species of the Cerrado used for food purposes and as herbal medicine. Currently, due to habitat loss and predatory extraction, there is a great demand in more studies for the conservation and use of this fruit species. The main challenge for the in vitro establishment of the species is the high number of endophytic agents (fungi and bacteria) that are present in plant tissues. This makes it more difficult to introduce plant material into the in vitro culture. This work aimed to optimize the in vitro establishment protocols in addition to carrying out vegetative propagation, via layering, generating clones of matrix plants. For the in vitro establishment of one of the varieties of the species, var. gardineri, first the fall fruits were collected in the Collection of Native Fruits of the Cerrado of the School of Agronomy at UFG. Fruits were pulped and 80 seeds were selected, which had their tegument removed and proceeded to the decontamination process. In a laminar flow chamber, two groups of seeds were separated: 40 seeds were opened and their embryos exposed, isolated and inoculated in MS culture medium; the other 40 seeds were inoculated directly in MS culture medium. To test the effect of stretching on reducing the period of development in vitro, embryos were inoculated in treatments with 0, 1, 2 and 4 μM of gibberellic acid (GA3) and later in a second experiment in MS medium with 1,0 μM of 6-benzylaminopurine (BAP), with lower concentrations of GA3: 0.2; 0.4; 0.6; 0.8 and 1 μM. For the next stage, treatments were carried out to root the new shoots with indolbutyric acid (IBA) in concentrations 0, 1, 3, 5 and 7 mg.mL-1 . The germination of the isolated embryos showed less than 5% of contamination and was 30% faster when compared to the seed. The effect of GA3 on isolated embryos allowed the plant to elongate with 0.8 μM, reaching 130 mm in 75 days, reducing in vitro cultivation by more than 100 days. In rooting, the concentration of 1 mg.mL-1 of AIB allowed more than 9 roots per plant and an average length of 54 mm. The acclimatization of the treatment plants took place on substrate with 50% sand and 50% broad red soil with commercial substrate. In this process, mortality was less than 10%. The layering experiments were carried out with four varieties of H. speciosa: gardineri, speciosa, cuyabensis and pubescens and in two different seasons: the dry season, and the rainy season. AIB was used to induce rooting at concentrations of 0, 4, 5 and 6 mg.mL-1 , with 15 ml per branch. The substrate used was soil humidified with commercial substrate fixed to the ringed branch. After 180 days, the best results were in the concentration of 5 mg.mL-1 in the four varieties used. However, the results for each variety were at different times of the year, due to the specific phenological phases. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-02-28 |
dc.date.accessioned.fl_str_mv |
2023-09-08T11:32:28Z |
dc.date.available.fl_str_mv |
2023-09-08T11:32:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TIAGO, B. S. Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes). 2020. 68 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/13035 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000000gks |
identifier_str_mv |
TIAGO, B. S. Propagação vegetativa por alporquia e otimização de protocolo de estabelecimento in vitro de mangabeira (Hancornia speciosa Gomes). 2020. 68 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2020. ark:/38995/0013000000gks |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/13035 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
60 |
dc.relation.confidence.fl_str_mv |
500 500 500 500 |
dc.relation.department.fl_str_mv |
2 |
dc.relation.cnpq.fl_str_mv |
651 |
dc.relation.sponsorship.fl_str_mv |
1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Genética e Melhoramento de Plantas (EA) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Agronomia - EA (RMG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
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Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
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tasesdissertacoes.bc@ufg.br |
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