Expressão de genes ortólogos relacionados à tolerância à seca em arroz

Detalhes bibliográficos
Autor(a) principal: Abreu, Fernanda Raquel Martins
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/001300000d8n4
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5454
Resumo: Drought, a major problem concerning a sustainable rice production in Brazil and worldwide, is responsible for a series of plant responses, including modification in gene expression, accumulation of metabolites and protein synthesis. In order to verify the correspondence between five Arabidopsis genes (PLDα1, LEW2, GluR2, Lsi1 e EIN2), previously related to drought tolerance, and their respective orthologs in rice, the present study analyzed two contrasting rice genotypes for drought, Douradão, the tolerant genotype, and Primavera, the susceptible one. The genotypes were submitted to drought stress and subsequently evaluated for gene expression by quantitative real-time Polymerase Chain Reaction (qPCR). The comparison of gene expression, between leaf and root tissues, showed a greater expression in roots, within their vegetative stage, and leaves, within their reproductive stage. Differential expression were observed mainly among the genes whose orthologs in Arabidopsis encode phospholipase Dα1 (PLDα1) and ethylene-insensitive protein (EIN2); these proteins are directly related to abscisic acid (ABA), a phytohormone that when identified in higher concentration in cells triggers the expression of drought stress-responsive genes, besides it is also responsible for the regulating the water loss (by transpiration) by controlling of stomatal movement. The results suggested that orthologs genes were in fact drought stress-responsive genes in rice, and emphasized the feasibility of PLDα1 and EIN2 overexpression in rice plants, supporting plant breeding programs in the development of drought tolerant genotypes.
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spelling Brondani, Claudiohttp://lattes.cnpq.br/4775600104554147Brondani, ClaudioPereira, MaristelaVianello, Rosana Pereirahttp://lattes.cnpq.br/6508170811705834Abreu, Fernanda Raquel Martins2016-04-06T11:34:39Z2014-04-04ABREU, F. R. M. Expressão de genes ortólogos relacionados à tolerância à seca em arroz. 2014. 76 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/5454ark:/38995/001300000d8n4Drought, a major problem concerning a sustainable rice production in Brazil and worldwide, is responsible for a series of plant responses, including modification in gene expression, accumulation of metabolites and protein synthesis. In order to verify the correspondence between five Arabidopsis genes (PLDα1, LEW2, GluR2, Lsi1 e EIN2), previously related to drought tolerance, and their respective orthologs in rice, the present study analyzed two contrasting rice genotypes for drought, Douradão, the tolerant genotype, and Primavera, the susceptible one. The genotypes were submitted to drought stress and subsequently evaluated for gene expression by quantitative real-time Polymerase Chain Reaction (qPCR). The comparison of gene expression, between leaf and root tissues, showed a greater expression in roots, within their vegetative stage, and leaves, within their reproductive stage. Differential expression were observed mainly among the genes whose orthologs in Arabidopsis encode phospholipase Dα1 (PLDα1) and ethylene-insensitive protein (EIN2); these proteins are directly related to abscisic acid (ABA), a phytohormone that when identified in higher concentration in cells triggers the expression of drought stress-responsive genes, besides it is also responsible for the regulating the water loss (by transpiration) by controlling of stomatal movement. The results suggested that orthologs genes were in fact drought stress-responsive genes in rice, and emphasized the feasibility of PLDα1 and EIN2 overexpression in rice plants, supporting plant breeding programs in the development of drought tolerant genotypes.A seca, um dos principais problemas para a sustentabilidade do cultivo de arroz no Brasil e no mundo, é responsável por uma série de respostas em plantas, incluindo mudanças na expressão gênica, acúmulo de metabólitos e síntese de proteínas. No intuito de verificar a correspondência entre cinco genes de Arabidopsis (PLDα1, LEW2, GluR2, Lsi1 e EIN2), previamente relacionados com a tolerância à seca, e seus respectivos genes ortólogos em arroz, o presente estudo analisou dois genótipos de arroz contrastantes no nível de tolerância à seca, Douradão, genótipo tolerante, e Primavera, genótipo susceptível. Os genótipos foram submetidos a experimento de seca e posterior avaliação de expressão gênica via PCR quantitativa em tempo real, qPCR (real time quantitative-Polimerase Chain Reaction). Ao se comparar os níveis de expressão entre os tecidos foliar e radicular, desses genótipos, foi observado, de uma forma geral, que houve maior expressão em raízes no estádio vegetativo e em folhas no estádio reprodutivo. Níveis diferenciais de expressão entre os genótipos foram observados principalmente para os genes ortólogos responsáveis por produzir fosfolipase Dα1 (PLDα1) e proteína etileno insensitiva (EIN2); estes produtos estão diretamente relacionados ao ácido abscísico (ABA), um fito hormônio induzido por estresse de seca que, quando em alta concentração nas células, além de acionar a expressão de muitos genes, desempenha um papel vital na regulação da perda de água por transpiração ao controlar os movimentos estomáticos. Os resultados apresentados nesta pesquisa sugeriram que os genes ortólogos estariam de fato atuando em resposta à tolerância à seca em arroz, e reforçaram a hipótese de que a superexpressão desses genes, em especial, PLDα1 e EIN2, pode auxiliar programas de melhoramento genético de arroz no desenvolvimento de cultivares mais tolerantes à seca.Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2016-04-05T19:37:55Z No. of bitstreams: 2 Dissertação - Fernanda Raquel Martins Abreu - 2014.pdf: 2480777 bytes, checksum: f8c50ff359945dffab56d7874ad25086 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-04-06T11:34:39Z (GMT) No. of bitstreams: 2 Dissertação - Fernanda Raquel Martins Abreu - 2014.pdf: 2480777 bytes, checksum: f8c50ff359945dffab56d7874ad25086 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-04-06T11:34:39Z (GMT). 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dc.title.por.fl_str_mv Expressão de genes ortólogos relacionados à tolerância à seca em arroz
dc.title.alternative.eng.fl_str_mv Expression of orthologs genes related to drought tolerance in rice
title Expressão de genes ortólogos relacionados à tolerância à seca em arroz
spellingShingle Expressão de genes ortólogos relacionados à tolerância à seca em arroz
Abreu, Fernanda Raquel Martins
ABA
Arabidopsis
Genes de referência
Genes responsivos à seca
qPCR
ABA
Arabidopsis
Drought stress-responsive genes
qPCR
Reference genes.
CIENCIAS AGRARIAS::AGRONOMIA
title_short Expressão de genes ortólogos relacionados à tolerância à seca em arroz
title_full Expressão de genes ortólogos relacionados à tolerância à seca em arroz
title_fullStr Expressão de genes ortólogos relacionados à tolerância à seca em arroz
title_full_unstemmed Expressão de genes ortólogos relacionados à tolerância à seca em arroz
title_sort Expressão de genes ortólogos relacionados à tolerância à seca em arroz
author Abreu, Fernanda Raquel Martins
author_facet Abreu, Fernanda Raquel Martins
author_role author
dc.contributor.advisor1.fl_str_mv Brondani, Claudio
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4775600104554147
dc.contributor.referee1.fl_str_mv Brondani, Claudio
dc.contributor.referee2.fl_str_mv Pereira, Maristela
dc.contributor.referee3.fl_str_mv Vianello, Rosana Pereira
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6508170811705834
dc.contributor.author.fl_str_mv Abreu, Fernanda Raquel Martins
contributor_str_mv Brondani, Claudio
Brondani, Claudio
Pereira, Maristela
Vianello, Rosana Pereira
dc.subject.por.fl_str_mv ABA
Arabidopsis
Genes de referência
Genes responsivos à seca
qPCR
topic ABA
Arabidopsis
Genes de referência
Genes responsivos à seca
qPCR
ABA
Arabidopsis
Drought stress-responsive genes
qPCR
Reference genes.
CIENCIAS AGRARIAS::AGRONOMIA
dc.subject.eng.fl_str_mv ABA
Arabidopsis
Drought stress-responsive genes
qPCR
Reference genes.
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::AGRONOMIA
description Drought, a major problem concerning a sustainable rice production in Brazil and worldwide, is responsible for a series of plant responses, including modification in gene expression, accumulation of metabolites and protein synthesis. In order to verify the correspondence between five Arabidopsis genes (PLDα1, LEW2, GluR2, Lsi1 e EIN2), previously related to drought tolerance, and their respective orthologs in rice, the present study analyzed two contrasting rice genotypes for drought, Douradão, the tolerant genotype, and Primavera, the susceptible one. The genotypes were submitted to drought stress and subsequently evaluated for gene expression by quantitative real-time Polymerase Chain Reaction (qPCR). The comparison of gene expression, between leaf and root tissues, showed a greater expression in roots, within their vegetative stage, and leaves, within their reproductive stage. Differential expression were observed mainly among the genes whose orthologs in Arabidopsis encode phospholipase Dα1 (PLDα1) and ethylene-insensitive protein (EIN2); these proteins are directly related to abscisic acid (ABA), a phytohormone that when identified in higher concentration in cells triggers the expression of drought stress-responsive genes, besides it is also responsible for the regulating the water loss (by transpiration) by controlling of stomatal movement. The results suggested that orthologs genes were in fact drought stress-responsive genes in rice, and emphasized the feasibility of PLDα1 and EIN2 overexpression in rice plants, supporting plant breeding programs in the development of drought tolerant genotypes.
publishDate 2014
dc.date.issued.fl_str_mv 2014-04-04
dc.date.accessioned.fl_str_mv 2016-04-06T11:34:39Z
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dc.identifier.citation.fl_str_mv ABREU, F. R. M. Expressão de genes ortólogos relacionados à tolerância à seca em arroz. 2014. 76 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2014.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/5454
dc.identifier.dark.fl_str_mv ark:/38995/001300000d8n4
identifier_str_mv ABREU, F. R. M. Expressão de genes ortólogos relacionados à tolerância à seca em arroz. 2014. 76 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2014.
ark:/38995/001300000d8n4
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publisher.none.fl_str_mv Universidade Federal de Goiás
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