Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/10717 |
Resumo: | Introduction: Thymol (Thy) is a monoterpene with biocidal activity against fungi, bacteria, some agricultural pests and mosquito larvae, and other applications. It is repeatedly used in high concentrations, due to its low stability with respect to temperature and light and by its rapid degradation in the environment. These facts can reduce exposure for non-target organisms. The application of nanotechnology by changing the physical-chemical characteristics of Thy and a controlled release system could reduce toxicity as a biocide. Objective: This study aimed to investigate and compare the acute toxicity and genotoxicity of the non-encapsulated Thy and its association in biogenic silica nanoparticles (BSiO2#Thy) on aquatic organisms. Methods: The acute toxicity test was performed with Artemia salina (Meyer et al., 1982 and OECD 202, 2004) and zebrafish (Danio rerio) early-life stage (OECD 236, 2013). The genotoxic effects were investigated in the comet assays with cells from zebrafish larvae (Kosmehl et al., 2006; Tice, 2000). Results: For A. salina test, the acute toxicity of Thy associated in a silica nanoparticle (BSiO2#Thy - LC50-48h = 1.06 ± 0.56 mg/L) remained practically unchanged in comparison to the non-encapsulated Thy (LC50- 48h = 1.03 ± 0.23 mg/L). The unloaded BSiO2 nanoparticle also induced significant mortality, but with less toxic potential (LC50-48h = 61.83 ± 0.43 mg/L). For zebrafish assay, the BSiO2 did not cause significant lethal, sublethal or genotoxic effects, even in high concentrations; however, the BSiO2#Thy was more toxic for the embryo-larval stage of zebrafish (LC50-96h = 13.13 mg/L) when compared to the non-encapsulated Thy (LC50-96h = 25.60 mg/L). In addition, BSiO2#Thy induced the same significant sublethal effects as the non-encapsulated Thy, such as defect in absorption of the yolk sac, pericardial edema and delayed hatching at 96h of exposure. Both formulations (BSiO2#Thy and Thy) were significantly genotoxic from 6.25 mg/L. Conclusion: Therefore, it can be concluded that the encapsulation of the Thy using silica nanoparticles increased acute toxicity for the zebrafish early-life stage and did not remove the toxic potential for A. salina nauplii and genotoxic for zebrafish larvae, since both effects remain unchanged. |
id |
UFG-2_2a4d1cfebe48c44a8c6ded2ca5bd7c51 |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/10717 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Oliveira, Gisele Augusto Rodrigues dehttp://lattes.cnpq.br/6221735418479539Oliveira, Gisele Augusto Rodrigues deTaveira, Stephânia FleurySilva, Gloria Narjara Santos dahttp://lattes.cnpq.br/1963306207676404Pereira, Iúri Barbosa2020-09-16T11:52:59Z2020-09-16T11:52:59Z2020-04-01PEREIRA, I. B. Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos. 2020. 63 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10717ark:/38995/0013000005315Introduction: Thymol (Thy) is a monoterpene with biocidal activity against fungi, bacteria, some agricultural pests and mosquito larvae, and other applications. It is repeatedly used in high concentrations, due to its low stability with respect to temperature and light and by its rapid degradation in the environment. These facts can reduce exposure for non-target organisms. The application of nanotechnology by changing the physical-chemical characteristics of Thy and a controlled release system could reduce toxicity as a biocide. Objective: This study aimed to investigate and compare the acute toxicity and genotoxicity of the non-encapsulated Thy and its association in biogenic silica nanoparticles (BSiO2#Thy) on aquatic organisms. Methods: The acute toxicity test was performed with Artemia salina (Meyer et al., 1982 and OECD 202, 2004) and zebrafish (Danio rerio) early-life stage (OECD 236, 2013). The genotoxic effects were investigated in the comet assays with cells from zebrafish larvae (Kosmehl et al., 2006; Tice, 2000). Results: For A. salina test, the acute toxicity of Thy associated in a silica nanoparticle (BSiO2#Thy - LC50-48h = 1.06 ± 0.56 mg/L) remained practically unchanged in comparison to the non-encapsulated Thy (LC50- 48h = 1.03 ± 0.23 mg/L). The unloaded BSiO2 nanoparticle also induced significant mortality, but with less toxic potential (LC50-48h = 61.83 ± 0.43 mg/L). For zebrafish assay, the BSiO2 did not cause significant lethal, sublethal or genotoxic effects, even in high concentrations; however, the BSiO2#Thy was more toxic for the embryo-larval stage of zebrafish (LC50-96h = 13.13 mg/L) when compared to the non-encapsulated Thy (LC50-96h = 25.60 mg/L). In addition, BSiO2#Thy induced the same significant sublethal effects as the non-encapsulated Thy, such as defect in absorption of the yolk sac, pericardial edema and delayed hatching at 96h of exposure. Both formulations (BSiO2#Thy and Thy) were significantly genotoxic from 6.25 mg/L. Conclusion: Therefore, it can be concluded that the encapsulation of the Thy using silica nanoparticles increased acute toxicity for the zebrafish early-life stage and did not remove the toxic potential for A. salina nauplii and genotoxic for zebrafish larvae, since both effects remain unchanged.Introdução: O timol (Thy) é um monoterpeno com atividade biocida contra fungos, bactérias, algumas pragas agrícolas e larvas de mosquitos, dentre outras aplicações. O Thy é usado em altas concentrações e de forma repetida, devido a sua baixa estabilidade com relação à temperatura e luminosidade e também pela rápida degradação no meio ambiente, podendo reduzir o seu contato com organismos não-alvo. A aplicação da nanotecnologia, através da alteração das características físico-químicas do Thy e de um sistema de liberação controlada poderiam reduzir os efeitos toxicos do biocida. Objetivo: Assim, esse trabalho teve como objetivo investigar e comparar a toxicidade aguda e genotoxicidade do timol não-encapsulado e associado em nanopartícula biogênica de sílica (BSiO2) sobre organismos aquáticos. Métodos: Para tanto foram realizados o teste de toxicidade aguda com Artemia salina (Meyer et al., 1982 e OECD 202, 2004), o teste de toxicidade aguda com embriões e larvas de zebrafish (Danio rerio) (OECD 236, 2013) seguido pelo ensaio do cometa (Kosmehl et al., 2006; Tice 2000). Resultados: Para A. salina, a toxicidade aguda do Thy encapsulado em nanopartícula biogênica de sílica (BSiO2#Thy - CL50-48h = 1,06 ± 0,56 mg/L) permaneceu praticamente inalterada em relação ao Thy não-encapsulado (CL50-48h = 1,03 ± 0,23 mg/L). A nanopartícula de BSiO2 não-carregada também induziu mortalidade significativa, mas com menor potencial tóxico (CE50-48h = 61,83 ± 0,43 mg/L). Para embriões e larvas de zebrafish, a BSiO2 não induziu efeitos letais, subletais ou genotóxicos significativos, mesmo em altas concentrações. Já o Thy nanoencapsulado (BSiO2#Thy) foi mais tóxico para o estágio embrio-larval de zebrafish (CL50-96h = 13,13 mg/L) quando comparado ao Thy não-encapsulado (CL50-96h = 25,60 mg/L). Além disso, o BSiO2#Thy induziu os mesmos efeitos subletais significativos do Thy não-carregado, como má absorção do saco vitelino, edema pericárdico e atraso na eclosão em 96h de exposição. Ambas as formulações (BSiO2#Thy e Thy) foram significativamente genotóxico a partir de 6,25 mg/L. Conclusões: Portanto, pode-se concluir que, a encapsulação do Thy em nanopartícula biogênica de sílica aumenta a toxicidade aguda para o estágio embrio-larval de zebrafish, e não remove o potencial tóxico para os náuplios de A. salina e genotóxico para as larvas de zebrafish, já que ambos efeitos permaneceram inalterados.Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2020-09-15T16:43:26Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Iúri Barbosa Pereira - 2020.pdf: 2278705 bytes, checksum: a3e18e0c52d9844f5f92202008cdc1b9 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-16T11:52:59Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Iúri Barbosa Pereira - 2020.pdf: 2278705 bytes, checksum: a3e18e0c52d9844f5f92202008cdc1b9 (MD5)Made available in DSpace on 2020-09-16T11:52:59Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Iúri Barbosa Pereira - 2020.pdf: 2278705 bytes, checksum: a3e18e0c52d9844f5f92202008cdc1b9 (MD5) Previous issue date: 2020-04-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Farmacêuticas (FF)UFGBrasilFaculdade Farmácia - FF (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessEcotoxicologiaZebrafishArtemia salinaEnsaio do cometaEcotoxicologyZebrafishArtemia salinaComet assayCIENCIAS DA SAUDE::FARMACIAAvaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticosToxicogenetic evaluation of the biocide thymol loaded in biogenic silica nanoparticles on aquatic organismsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis26500500500500231751reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/de8be3a1-bc15-44e1-83fb-37a19a85273a/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/af654610-2501-4182-858d-ae47acdb7d54/downloade39d27027a6cc9cb039ad269a5db8e34MD52ORIGINALDissertação - Iúri Barbosa Pereira - 2020.pdfDissertação - Iúri Barbosa Pereira - 2020.pdfapplication/pdf2278705http://repositorio.bc.ufg.br/tede/bitstreams/64644eba-b9b3-422b-a755-28ac00b054e9/downloada3e18e0c52d9844f5f92202008cdc1b9MD53tede/107172020-09-16 08:53:00.356http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10717http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-09-16T11:53Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
dc.title.alternative.eng.fl_str_mv |
Toxicogenetic evaluation of the biocide thymol loaded in biogenic silica nanoparticles on aquatic organisms |
title |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
spellingShingle |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos Pereira, Iúri Barbosa Ecotoxicologia Zebrafish Artemia salina Ensaio do cometa Ecotoxicology Zebrafish Artemia salina Comet assay CIENCIAS DA SAUDE::FARMACIA |
title_short |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
title_full |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
title_fullStr |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
title_full_unstemmed |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
title_sort |
Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos |
author |
Pereira, Iúri Barbosa |
author_facet |
Pereira, Iúri Barbosa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Gisele Augusto Rodrigues de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6221735418479539 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Gisele Augusto Rodrigues de |
dc.contributor.referee2.fl_str_mv |
Taveira, Stephânia Fleury |
dc.contributor.referee3.fl_str_mv |
Silva, Gloria Narjara Santos da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1963306207676404 |
dc.contributor.author.fl_str_mv |
Pereira, Iúri Barbosa |
contributor_str_mv |
Oliveira, Gisele Augusto Rodrigues de Oliveira, Gisele Augusto Rodrigues de Taveira, Stephânia Fleury Silva, Gloria Narjara Santos da |
dc.subject.por.fl_str_mv |
Ecotoxicologia Zebrafish Artemia salina Ensaio do cometa |
topic |
Ecotoxicologia Zebrafish Artemia salina Ensaio do cometa Ecotoxicology Zebrafish Artemia salina Comet assay CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Ecotoxicology Zebrafish Artemia salina Comet assay |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA |
description |
Introduction: Thymol (Thy) is a monoterpene with biocidal activity against fungi, bacteria, some agricultural pests and mosquito larvae, and other applications. It is repeatedly used in high concentrations, due to its low stability with respect to temperature and light and by its rapid degradation in the environment. These facts can reduce exposure for non-target organisms. The application of nanotechnology by changing the physical-chemical characteristics of Thy and a controlled release system could reduce toxicity as a biocide. Objective: This study aimed to investigate and compare the acute toxicity and genotoxicity of the non-encapsulated Thy and its association in biogenic silica nanoparticles (BSiO2#Thy) on aquatic organisms. Methods: The acute toxicity test was performed with Artemia salina (Meyer et al., 1982 and OECD 202, 2004) and zebrafish (Danio rerio) early-life stage (OECD 236, 2013). The genotoxic effects were investigated in the comet assays with cells from zebrafish larvae (Kosmehl et al., 2006; Tice, 2000). Results: For A. salina test, the acute toxicity of Thy associated in a silica nanoparticle (BSiO2#Thy - LC50-48h = 1.06 ± 0.56 mg/L) remained practically unchanged in comparison to the non-encapsulated Thy (LC50- 48h = 1.03 ± 0.23 mg/L). The unloaded BSiO2 nanoparticle also induced significant mortality, but with less toxic potential (LC50-48h = 61.83 ± 0.43 mg/L). For zebrafish assay, the BSiO2 did not cause significant lethal, sublethal or genotoxic effects, even in high concentrations; however, the BSiO2#Thy was more toxic for the embryo-larval stage of zebrafish (LC50-96h = 13.13 mg/L) when compared to the non-encapsulated Thy (LC50-96h = 25.60 mg/L). In addition, BSiO2#Thy induced the same significant sublethal effects as the non-encapsulated Thy, such as defect in absorption of the yolk sac, pericardial edema and delayed hatching at 96h of exposure. Both formulations (BSiO2#Thy and Thy) were significantly genotoxic from 6.25 mg/L. Conclusion: Therefore, it can be concluded that the encapsulation of the Thy using silica nanoparticles increased acute toxicity for the zebrafish early-life stage and did not remove the toxic potential for A. salina nauplii and genotoxic for zebrafish larvae, since both effects remain unchanged. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-16T11:52:59Z |
dc.date.available.fl_str_mv |
2020-09-16T11:52:59Z |
dc.date.issued.fl_str_mv |
2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, I. B. Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos. 2020. 63 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10717 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000005315 |
identifier_str_mv |
PEREIRA, I. B. Avaliação toxicogenetica do biocida timol encapsulado em nanopartícula biogênica de sílica sobre organismos aquáticos. 2020. 63 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2020. ark:/38995/0013000005315 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10717 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
26 |
dc.relation.confidence.fl_str_mv |
500 500 500 500 |
dc.relation.department.fl_str_mv |
23 |
dc.relation.cnpq.fl_str_mv |
175 |
dc.relation.sponsorship.fl_str_mv |
1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciências Farmacêuticas (FF) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade Farmácia - FF (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/de8be3a1-bc15-44e1-83fb-37a19a85273a/download http://repositorio.bc.ufg.br/tede/bitstreams/af654610-2501-4182-858d-ae47acdb7d54/download http://repositorio.bc.ufg.br/tede/bitstreams/64644eba-b9b3-422b-a755-28ac00b054e9/download |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 e39d27027a6cc9cb039ad269a5db8e34 a3e18e0c52d9844f5f92202008cdc1b9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1811721394690457600 |