Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Philippsen, Hellen Kempfer
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/001300000936g
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/3222
Resumo: Paracoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways.
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spelling Soares, Célia Maria de Almeidahttp://lattes.cnpq.br/8539946335852637Soares, Célia Maria de AlmeidaKipnis, AndréPereira, MaristelaBailão, Alexandre MeloBorges, Clayton Luíshttp://lattes.cnpq.br/9963999800396051Philippsen, Hellen Kempfer2014-09-30T15:28:08Z2011-08-30PHILIPPSEN, Hellen Kempfer. Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis. 2011. 57 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2011.http://repositorio.bc.ufg.br/tede/handle/tede/3222ark:/38995/001300000936gParacoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways.Paracoccidioides brasiliensis é um fungo patogênico termodimórfico, agente causador da Paracoccidioidomicose (PCM), uma micose sistêmica que afeta principalmente indivíduos da América Latina. Muitos fatores são necessários para que P. brasiliensis estabeleça infecção, como o remodelamento da parede celular e a capacidade de captar nutrientes do hospedeiro para seu metabolismo. Como o íon ferro é um cofator de diversas reações, o hospedeiro diminui a disponibilidade de íons ferro na forma livre para o patógeno. Portanto, o presente trabalho visa investigar o perfil de proteínas da parede celular de P. brasiliensis quando em condição de depleção do íon ferro. Para esse fim, realizou-se a extração das proteínas da parede das células de P. brasiliensis em meio com restrição do íon ferro. O perfil proteômico das condições experimental e controle foram obtidos por eletroforese bidimensional com posterior análise de imagens. Análises estatísticas demonstraram as proteínas reguladas nas condições de depleção de ferro, que foram submetidas à digestão tríptica e identificação por espectrometria de massas. Foram identificadas 26 proteínas por perfil de digestão tríptica (PMF) e/ou perfil de fragmentação dos íons (MS/MS). A maioria dessas proteínas apresenta preditas modificações pós - traducionais e apenas algumas apresentam peptídeo sinal em suas sequências, o que sugere que elas são endereçadas à superfície por vias não clássicas.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-30T12:45:52Z No. of bitstreams: 2 Philippsen, Hellen Kempfer-2011-dissertação.pdf: 1457910 bytes, checksum: 4ee585b7a28a164d44414a0a2ef76ae5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-30T15:28:08Z (GMT) No. of bitstreams: 2 Philippsen, Hellen Kempfer-2011-dissertação.pdf: 1457910 bytes, checksum: 4ee585b7a28a164d44414a0a2ef76ae5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-09-30T15:28:08Z (GMT). No. of bitstreams: 2 Philippsen, Hellen Kempfer-2011-dissertação.pdf: 1457910 bytes, checksum: 4ee585b7a28a164d44414a0a2ef76ae5 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2011-08-30Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/9102/Philippsen%2c%20Hellen%20Kempfer-2011-disserta%c3%a7%c3%a3o.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)1. Abdul, R.A.; Oellerich, M.; Amstrong, V.W.; Riemenschneider, B.; Reichard, U. (2006). Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens. Proteome research. 5, 954-962. 2. Almeida, F.B.D.R.; Carvalho, F.C.; Mariano, V.S.; Alegre, A.C.P.; Silva, R.N.; Hanna, E.S.; Roque-Barreira, M.C. (2011). 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dc.title.por.fl_str_mv Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
dc.title.alternative.eng.fl_str_mv Influence of iron in the composition of surface protein non-covalently bound of paracoccidioides brasiliensis
title Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
spellingShingle Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
Philippsen, Hellen Kempfer
Paracoccidioides brasiliensis
Parede celular
Paracoccidioidomicose
Deficiência de ferro
Iron
Cell wall
Protein
MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA
title_short Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
title_full Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
title_fullStr Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
title_full_unstemmed Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
title_sort Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis
author Philippsen, Hellen Kempfer
author_facet Philippsen, Hellen Kempfer
author_role author
dc.contributor.advisor1.fl_str_mv Soares, Célia Maria de Almeida
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8539946335852637
dc.contributor.referee1.fl_str_mv Soares, Célia Maria de Almeida
dc.contributor.referee2.fl_str_mv Kipnis, André
dc.contributor.referee3.fl_str_mv Pereira, Maristela
dc.contributor.referee4.fl_str_mv Bailão, Alexandre Melo
dc.contributor.referee5.fl_str_mv Borges, Clayton Luís
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9963999800396051
dc.contributor.author.fl_str_mv Philippsen, Hellen Kempfer
contributor_str_mv Soares, Célia Maria de Almeida
Soares, Célia Maria de Almeida
Kipnis, André
Pereira, Maristela
Bailão, Alexandre Melo
Borges, Clayton Luís
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
Parede celular
Paracoccidioidomicose
Deficiência de ferro
topic Paracoccidioides brasiliensis
Parede celular
Paracoccidioidomicose
Deficiência de ferro
Iron
Cell wall
Protein
MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA
dc.subject.eng.fl_str_mv Iron
Cell wall
Protein
dc.subject.cnpq.fl_str_mv MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA
description Paracoccidioides brasiliensis is a dimorphic fungus, the causative agent of paracoccidioidomycosis (PCM), a systemic mycosis that affects mainly people from Latin America. In order to establish infection, many factors are required for P. brasiliensis, such as cell wall remodeling and the ability to capture nutrients from the host. As iron is an important cofactor of many reactions, the host reduces the availability of free iron ions to the pathogen. Therefore, this study aims to elucidate the proteomic profile of the cell wall in response to iron depletion. The extraction of surface proteins of the cell wall in iron restriction and iron replete conditions was performed and the proteomic profile was obtained by two-dimensional electrophoresis with subsequent analysis of images. Statistical analysis demonstrated proteins with differential level of expression in each condition, which were subjected to tryptic digestion and identification by mass spectrometry. It was identified 26 proteins by peptide mass fingerprint (PMF) and/or ions fragmentation (MS/MS). The majority of these proteins have post - translational modifications and only some have in their sequences signal peptide, suggesting that they are addressed to the surface by non-classical pathways.
publishDate 2011
dc.date.issued.fl_str_mv 2011-08-30
dc.date.accessioned.fl_str_mv 2014-09-30T15:28:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PHILIPPSEN, Hellen Kempfer. Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis. 2011. 57 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2011.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/3222
dc.identifier.dark.fl_str_mv ark:/38995/001300000936g
identifier_str_mv PHILIPPSEN, Hellen Kempfer. Influência de ferro na composição de proteínas de superfície não covalentemente ligadas de paracoccidioides brasiliensis. 2011. 57 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2011.
ark:/38995/001300000936g
url http://repositorio.bc.ufg.br/tede/handle/tede/3222
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 6085308344741430434
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -7769011444564556288
dc.relation.cnpq.fl_str_mv 8648187577661350585
dc.relation.sponsorship.fl_str_mv -2555911436985713659
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