Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
dARK ID: | ark:/38995/0013000004k2f |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/10745 |
Resumo: | Introduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair. |
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Vulcani, Valcinir Aloisio Scallahttp://lattes.cnpq.br/0967468447941793Moraes, Julia de Mirandahttp://lattes.cnpq.br/7729197454739642Vulcani, Valcinir Aloisio ScallaPrado, Rodrigo PaschoalAraújo, Gustavo Henrique MarquesSaturnino, Klaus CasaroRita, Ricardo de Mattos Santahttp://lattes.cnpq.br/0967468447941793Ribeiro, Maisa2020-09-17T15:15:16Z2020-09-17T15:15:16Z2020-07-31RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10745ark:/38995/0013000004k2fIntroduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.Introdução: Lesões provocadas por queimadura de espessura total são consideradas como uma forma severa de trauma, com elevado índice de morbimortalidade mundialmente, capaz de promover destruição do tecido cutâneo e comprometer morfuncionalmente o organismo. O uso de células-tronco derivadas do tecido adiposo (ADSC) e laser de baixa potência (LBP) são alternativas terapêuticas promissoras exploradas pela medicina regenerativa. O objetivo deste estudo foi estabelecer um modelo de isolamento eficaz para células ADSC mais homogêneas; avaliar os efeitos das ADSC e LBP no processo de cicatrização cutânea e investigar a associação das técnicas (LBP e ADSC) no tratamento para queimadura de espessura total em modelo experimental de rato. Material e métodos: As obtenções das ADSC foram realizadas por meio do isolamento imonumagnético por proteína CD49d e cultivo convencional. Um total de 150 ratos Wistar foram divididos em grupo controle e grupos tratados com ADSC CD49d positiva, ADSC CD49d negativa, células-tronco de isolamento convencional (CULT), LBP, ADSC+LBP e ADSC-LBP. Foram induzidas queimaduras por escaldadura no dorso de cada animal, com posterior debridamento cirúrgico. Utilizou-se LBP com comprimento de onda de 660 nm, 30 mW de potência em cinco pontos da ferida, sob fluência total de 450 Joules/cm2 e aplicação total de 5 Joules. Foram realizadas análises macroscópicas e histopatológicas de acordo com biópsias coletadas nos dias 5, 14 e 21 pós-lesão, processadas e coradas pelas técnicas de Hematoxilina e Eosina (HE) e Tricrômico de Gomori (TG). As análises estatísticas foram feitas por Teste de Tukey e análise de Variância (ANOVA), Teste de Kruskal-Wallis (não-paramétrico) e Teste de T-Pareado. Para todas as análises empregou-se o nível de significância de 5% (p<0,05). Resultados: Os resultados apontaram diferença estatística na morfometria entre os grupos com melhor grau de reepitelização e diminuição da área de lesão apresentado pelos tratamentos ADSC+ e CULT aos 21 dias de biópsia. A análise histopatológica evidenciou que os tratamentos ADSC+, LBP e ADSC+LBP influenciaram significativamente o processo de cicatrização apresentando melhora quanto ao aumento de infiltrados inflamatórios mononuclear, maior angiogênese, diminuição do edema, aumento no tecido de granulação, no número de fibroblastos no início do reparo tecidual, além de promover reepitelização de forma mais organizada com maior deposição de colágeno e formação de anexos epiteliais aos 21 dias após a lesão. Conclusão: A fotobiomodulação por laser de baixa potência das células ADSC potencializou o processo de reparo em feridas por queimadura de espessura total demonstrando uma interação significativa para o remodelamento da matriz extracelular e tecido cutâneo. Estudos adicionais são necessários para mensurar os efeitos do LBP e a atividade das ADSCs no reparo do tecido cutâneo.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-17T12:34:32Z No. of bitstreams: 2 Tese - Maisa Ribeiro - 2020.pdf: 4130049 bytes, checksum: f0b8a18ce8ebcb127ec1ebb7e14216d6 (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-17T15:15:16Z (GMT) No. of bitstreams: 2 Tese - Maisa Ribeiro - 2020.pdf: 4130049 bytes, checksum: f0b8a18ce8ebcb127ec1ebb7e14216d6 (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)Made available in DSpace on 2020-09-17T15:15:16Z (GMT). No. of bitstreams: 2 Tese - Maisa Ribeiro - 2020.pdf: 4130049 bytes, checksum: f0b8a18ce8ebcb127ec1ebb7e14216d6 (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Previous issue date: 2020-07-31porUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCélulas-troncoTecido adiposoQueimaduraFotobiomodulaçãoStem cellsAdipose tissueBurnPhotobiomodulationCIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIAEfeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratosEffect of photobiomodulation and stem cells derived from adipose tissue on the repair of wounds by thermal burn in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis185005005006241reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALTese - Maisa Ribeiro - 2020.pdfTese - Maisa Ribeiro - 2020.pdfapplication/pdf4130049http://repositorio.bc.ufg.br/tede/bitstreams/3cbe6d4f-9e28-4fd8-aaf1-bd39fc6f682d/downloadf0b8a18ce8ebcb127ec1ebb7e14216d6MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/75e0528c-6047-4d08-80ad-e2c54c115b52/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/6fe80661-fbc5-4e4b-8136-2a1e395ac2f6/downloade39d27027a6cc9cb039ad269a5db8e34MD52tede/107452020-09-17 12:15:16.755http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10745http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-09-17T15:15:16Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)falseTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |
dc.title.pt_BR.fl_str_mv |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
dc.title.alternative.eng.fl_str_mv |
Effect of photobiomodulation and stem cells derived from adipose tissue on the repair of wounds by thermal burn in rats |
title |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
spellingShingle |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos Ribeiro, Maisa Células-tronco Tecido adiposo Queimadura Fotobiomodulação Stem cells Adipose tissue Burn Photobiomodulation CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA |
title_short |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
title_full |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
title_fullStr |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
title_full_unstemmed |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
title_sort |
Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos |
author |
Ribeiro, Maisa |
author_facet |
Ribeiro, Maisa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vulcani, Valcinir Aloisio Scalla |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0967468447941793 |
dc.contributor.advisor-co1.fl_str_mv |
Moraes, Julia de Miranda |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7729197454739642 |
dc.contributor.referee1.fl_str_mv |
Vulcani, Valcinir Aloisio Scalla |
dc.contributor.referee2.fl_str_mv |
Prado, Rodrigo Paschoal |
dc.contributor.referee3.fl_str_mv |
Araújo, Gustavo Henrique Marques |
dc.contributor.referee4.fl_str_mv |
Saturnino, Klaus Casaro |
dc.contributor.referee5.fl_str_mv |
Rita, Ricardo de Mattos Santa |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0967468447941793 |
dc.contributor.author.fl_str_mv |
Ribeiro, Maisa |
contributor_str_mv |
Vulcani, Valcinir Aloisio Scalla Moraes, Julia de Miranda Vulcani, Valcinir Aloisio Scalla Prado, Rodrigo Paschoal Araújo, Gustavo Henrique Marques Saturnino, Klaus Casaro Rita, Ricardo de Mattos Santa |
dc.subject.por.fl_str_mv |
Células-tronco Tecido adiposo Queimadura Fotobiomodulação |
topic |
Células-tronco Tecido adiposo Queimadura Fotobiomodulação Stem cells Adipose tissue Burn Photobiomodulation CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA |
dc.subject.eng.fl_str_mv |
Stem cells Adipose tissue Burn Photobiomodulation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA |
description |
Introduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-17T15:15:16Z |
dc.date.available.fl_str_mv |
2020-09-17T15:15:16Z |
dc.date.issued.fl_str_mv |
2020-07-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10745 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000004k2f |
identifier_str_mv |
RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020. ark:/38995/0013000004k2f |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10745 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
18 |
dc.relation.confidence.fl_str_mv |
500 500 500 |
dc.relation.department.fl_str_mv |
6 |
dc.relation.cnpq.fl_str_mv |
241 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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tasesdissertacoes.bc@ufg.br |
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