Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Maisa
Data de Publicação: 2020
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/0013000004k2f
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/10745
Resumo: Introduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.
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spelling Vulcani, Valcinir Aloisio Scallahttp://lattes.cnpq.br/0967468447941793Moraes, Julia de Mirandahttp://lattes.cnpq.br/7729197454739642Vulcani, Valcinir Aloisio ScallaPrado, Rodrigo PaschoalAraújo, Gustavo Henrique MarquesSaturnino, Klaus CasaroRita, Ricardo de Mattos Santahttp://lattes.cnpq.br/0967468447941793Ribeiro, Maisa2020-09-17T15:15:16Z2020-09-17T15:15:16Z2020-07-31RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10745ark:/38995/0013000004k2fIntroduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.Introdução: Lesões provocadas por queimadura de espessura total são consideradas como uma forma severa de trauma, com elevado índice de morbimortalidade mundialmente, capaz de promover destruição do tecido cutâneo e comprometer morfuncionalmente o organismo. O uso de células-tronco derivadas do tecido adiposo (ADSC) e laser de baixa potência (LBP) são alternativas terapêuticas promissoras exploradas pela medicina regenerativa. O objetivo deste estudo foi estabelecer um modelo de isolamento eficaz para células ADSC mais homogêneas; avaliar os efeitos das ADSC e LBP no processo de cicatrização cutânea e investigar a associação das técnicas (LBP e ADSC) no tratamento para queimadura de espessura total em modelo experimental de rato. Material e métodos: As obtenções das ADSC foram realizadas por meio do isolamento imonumagnético por proteína CD49d e cultivo convencional. Um total de 150 ratos Wistar foram divididos em grupo controle e grupos tratados com ADSC CD49d positiva, ADSC CD49d negativa, células-tronco de isolamento convencional (CULT), LBP, ADSC+LBP e ADSC-LBP. Foram induzidas queimaduras por escaldadura no dorso de cada animal, com posterior debridamento cirúrgico. Utilizou-se LBP com comprimento de onda de 660 nm, 30 mW de potência em cinco pontos da ferida, sob fluência total de 450 Joules/cm2 e aplicação total de 5 Joules. Foram realizadas análises macroscópicas e histopatológicas de acordo com biópsias coletadas nos dias 5, 14 e 21 pós-lesão, processadas e coradas pelas técnicas de Hematoxilina e Eosina (HE) e Tricrômico de Gomori (TG). As análises estatísticas foram feitas por Teste de Tukey e análise de Variância (ANOVA), Teste de Kruskal-Wallis (não-paramétrico) e Teste de T-Pareado. Para todas as análises empregou-se o nível de significância de 5% (p<0,05). Resultados: Os resultados apontaram diferença estatística na morfometria entre os grupos com melhor grau de reepitelização e diminuição da área de lesão apresentado pelos tratamentos ADSC+ e CULT aos 21 dias de biópsia. A análise histopatológica evidenciou que os tratamentos ADSC+, LBP e ADSC+LBP influenciaram significativamente o processo de cicatrização apresentando melhora quanto ao aumento de infiltrados inflamatórios mononuclear, maior angiogênese, diminuição do edema, aumento no tecido de granulação, no número de fibroblastos no início do reparo tecidual, além de promover reepitelização de forma mais organizada com maior deposição de colágeno e formação de anexos epiteliais aos 21 dias após a lesão. Conclusão: A fotobiomodulação por laser de baixa potência das células ADSC potencializou o processo de reparo em feridas por queimadura de espessura total demonstrando uma interação significativa para o remodelamento da matriz extracelular e tecido cutâneo. Estudos adicionais são necessários para mensurar os efeitos do LBP e a atividade das ADSCs no reparo do tecido cutâneo.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-17T12:34:32Z No. of bitstreams: 2 Tese - Maisa Ribeiro - 2020.pdf: 4130049 bytes, checksum: f0b8a18ce8ebcb127ec1ebb7e14216d6 (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-17T15:15:16Z (GMT) No. of bitstreams: 2 Tese - Maisa Ribeiro - 2020.pdf: 4130049 bytes, checksum: f0b8a18ce8ebcb127ec1ebb7e14216d6 (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)Made available in DSpace on 2020-09-17T15:15:16Z (GMT). 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dc.title.pt_BR.fl_str_mv Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
dc.title.alternative.eng.fl_str_mv Effect of photobiomodulation and stem cells derived from adipose tissue on the repair of wounds by thermal burn in rats
title Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
spellingShingle Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
Ribeiro, Maisa
Células-tronco
Tecido adiposo
Queimadura
Fotobiomodulação
Stem cells
Adipose tissue
Burn
Photobiomodulation
CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
title_short Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
title_full Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
title_fullStr Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
title_full_unstemmed Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
title_sort Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
author Ribeiro, Maisa
author_facet Ribeiro, Maisa
author_role author
dc.contributor.advisor1.fl_str_mv Vulcani, Valcinir Aloisio Scalla
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0967468447941793
dc.contributor.advisor-co1.fl_str_mv Moraes, Julia de Miranda
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7729197454739642
dc.contributor.referee1.fl_str_mv Vulcani, Valcinir Aloisio Scalla
dc.contributor.referee2.fl_str_mv Prado, Rodrigo Paschoal
dc.contributor.referee3.fl_str_mv Araújo, Gustavo Henrique Marques
dc.contributor.referee4.fl_str_mv Saturnino, Klaus Casaro
dc.contributor.referee5.fl_str_mv Rita, Ricardo de Mattos Santa
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0967468447941793
dc.contributor.author.fl_str_mv Ribeiro, Maisa
contributor_str_mv Vulcani, Valcinir Aloisio Scalla
Moraes, Julia de Miranda
Vulcani, Valcinir Aloisio Scalla
Prado, Rodrigo Paschoal
Araújo, Gustavo Henrique Marques
Saturnino, Klaus Casaro
Rita, Ricardo de Mattos Santa
dc.subject.por.fl_str_mv Células-tronco
Tecido adiposo
Queimadura
Fotobiomodulação
topic Células-tronco
Tecido adiposo
Queimadura
Fotobiomodulação
Stem cells
Adipose tissue
Burn
Photobiomodulation
CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
dc.subject.eng.fl_str_mv Stem cells
Adipose tissue
Burn
Photobiomodulation
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA
description Introduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-09-17T15:15:16Z
dc.date.available.fl_str_mv 2020-09-17T15:15:16Z
dc.date.issued.fl_str_mv 2020-07-31
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/10745
dc.identifier.dark.fl_str_mv ark:/38995/0013000004k2f
identifier_str_mv RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.
ark:/38995/0013000004k2f
url http://repositorio.bc.ufg.br/tede/handle/tede/10745
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 18
dc.relation.confidence.fl_str_mv 500
500
500
dc.relation.department.fl_str_mv 6
dc.relation.cnpq.fl_str_mv 241
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciência Animal (EVZ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Repositório Institucional da UFG
collection Repositório Institucional da UFG
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http://repositorio.bc.ufg.br/tede/bitstreams/75e0528c-6047-4d08-80ad-e2c54c115b52/download
http://repositorio.bc.ufg.br/tede/bitstreams/6fe80661-fbc5-4e4b-8136-2a1e395ac2f6/download
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bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
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