Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento

Detalhes bibliográficos
Autor(a) principal: Veras, Poliana Ribeiro Valadares
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/0013000002zpz
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/6069
Resumo: American tegumentary leishmaniasis (ATL) is an infectious parasitic disease caused by Leishmania protozoa. The disease presents as cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML). The mechanisms of the immunopathogenesis and infection control are not yet fully understood, and monocytes can be involved in these mechanisms. The monocytes are divided into three subsets (CD14hiCD16-, classical monocytes; CD14hiCD16+, the intermediates; and CD14loCD16+ non- classical) and are the major cell producing cytokines in peripheral blood.The objective of this study was to assess possible alterations in the percentages of monocytes subsets; and in the tumor necrosis factor (TNF) and interleukin 10 (IL -10) production in whole blood cultures from patients with CL and ML, before and after treatment. Peripheral blood from patients (n = 54; 31 CL and ML 23) and controls (n = 54) was used to identify monocytes by flow cytometry and for whole blood cultures. The blood was incubated in the absence (medium) or presence of toll-like receptor agonists (Pam3Cys and LPS for TLR2 and TLR4, respectively) or L. (V.) braziliensisantigens (AG) to assess TNF and IL-10. Cytokines were quantified by enzyme-linked immunosorbent assay (ELISA).The results showed an increase in the percentage of CD16+ monocytes, especially CD14loCD16+monocyte subset in CL patients, but not in ML, before treatment (p <0.05). After treatment, the percentages of these monocytes in CL patients back to similar levels of those from healthy individuals. In patients with ML, there was also a reduction in the percentages of CD16+ monocytes after treatment. The production of TNF and IL-10 was not significantly altered in whole blood cultures from patients, compared with those from healthy controls. Among the stimuli used, only the AG did not induce significant amounts of IL-10 in whole blood cultures from patients. After treatment, TNF concentrations decreased in CL whole blood cultures, except when the stimulus was Pam3Cys (TLR2), which induced an increase in TNF levels (p <0.05). In ML whole blood cultures no significant differences were detected between the concentrations of TNF and IL-10 produced before and after treatment.The IL-10 concentrations were not significantly altered after treatment of CL patients. The data indicate that percentages of CD16+ monocytes are increased in CL. Also, they suggest that monocytes from patients with CL or ML show a decreased capacity to produce IL-10 in response to AG, what can hamper the control of the inflammatory response. The data also suggest that the ability of monocytes to be activatedthrough TLR2 can be suppressed in CL what isrecovered after treatment. An analysis of TLR2 and TLR4 in monocytes from patients with ATL can improve the knowledge about cytokine induction in these cells.
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spelling Dias, Fátima Ribeirohttp://lattes.cnpq.br/5741031258926403Dorta, Miriam LeandroGomes, Rodrigo Saarhttp://lattes.cnpq.br/3032438721919576Veras, Poliana Ribeiro Valadares2016-09-01T11:58:38Z2015-07-02VERAS, P. R. V. Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento. 2015. 56 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/6069ark:/38995/0013000002zpzAmerican tegumentary leishmaniasis (ATL) is an infectious parasitic disease caused by Leishmania protozoa. The disease presents as cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML). The mechanisms of the immunopathogenesis and infection control are not yet fully understood, and monocytes can be involved in these mechanisms. The monocytes are divided into three subsets (CD14hiCD16-, classical monocytes; CD14hiCD16+, the intermediates; and CD14loCD16+ non- classical) and are the major cell producing cytokines in peripheral blood.The objective of this study was to assess possible alterations in the percentages of monocytes subsets; and in the tumor necrosis factor (TNF) and interleukin 10 (IL -10) production in whole blood cultures from patients with CL and ML, before and after treatment. Peripheral blood from patients (n = 54; 31 CL and ML 23) and controls (n = 54) was used to identify monocytes by flow cytometry and for whole blood cultures. The blood was incubated in the absence (medium) or presence of toll-like receptor agonists (Pam3Cys and LPS for TLR2 and TLR4, respectively) or L. (V.) braziliensisantigens (AG) to assess TNF and IL-10. Cytokines were quantified by enzyme-linked immunosorbent assay (ELISA).The results showed an increase in the percentage of CD16+ monocytes, especially CD14loCD16+monocyte subset in CL patients, but not in ML, before treatment (p <0.05). After treatment, the percentages of these monocytes in CL patients back to similar levels of those from healthy individuals. In patients with ML, there was also a reduction in the percentages of CD16+ monocytes after treatment. The production of TNF and IL-10 was not significantly altered in whole blood cultures from patients, compared with those from healthy controls. Among the stimuli used, only the AG did not induce significant amounts of IL-10 in whole blood cultures from patients. After treatment, TNF concentrations decreased in CL whole blood cultures, except when the stimulus was Pam3Cys (TLR2), which induced an increase in TNF levels (p <0.05). In ML whole blood cultures no significant differences were detected between the concentrations of TNF and IL-10 produced before and after treatment.The IL-10 concentrations were not significantly altered after treatment of CL patients. The data indicate that percentages of CD16+ monocytes are increased in CL. Also, they suggest that monocytes from patients with CL or ML show a decreased capacity to produce IL-10 in response to AG, what can hamper the control of the inflammatory response. The data also suggest that the ability of monocytes to be activatedthrough TLR2 can be suppressed in CL what isrecovered after treatment. An analysis of TLR2 and TLR4 in monocytes from patients with ATL can improve the knowledge about cytokine induction in these cells.A leishmaniose tegumentar americana (LTA) é uma doença infecto-parasitária causada por protozoários do gênero Leishmania. Esta doença apresenta-se como leishmaniose cutânea (LC) e leishmaniose mucosa (LM). Os mecanismos da imunopatogenia e do controle da infecção ainda não estão completamente esclarecidos, sendo que os monócitos podem participar destes mecanismos. Os monócitos são subdivididos em três populações (CD14hiCD16-, os clássicos; CD14hiCD16+, os intermediários; e CD14loCD16+, os não clássicos) e são as principais células produtoras de citocinas no sangue periférico. O objetivo deste estudo foi avaliar se existem alterações nas proporções das três subpopulações dos monócitos e das citocinas fator de necrose tumoral (TNF) e interleucina 10 (IL-10), em pacientes com LC ou LM, antes e após o tratamento. Sangue periférico de pacientes (n = 54; 31 LC e 23 LM) e controles (n = 54) foi utilizado para identificar os monócitos por citometria de fluxo e para hemoculturas. As hemoculturas foram incubadas na ausência (Meio) ou presença de agonistas (LPS, Pam3Cys) de receptores similares a toll dos tipos dois e quatro (TLR2 e TLR4) ou de antígenos (AG) de L. (Viannia) braziliensis, para avaliar a produção de TNF e IL-10. As citocinas foram quantificadas por ensaio imunoenzimático (ELISA). Os resultados mostraram um aumento da porcentagem dos monócitos CD16+, especialmente da subpopulação CD14loCD16+, em pacientes com LC, mas não com LM, antes do tratamento (p < 0,05). Após o tratamento, as porcentagens destes monócitos, em pacientes com LC, retornaram para níveis similares àqueles de indivíduos sadios. Em pacientes com LM, também ocorreu uma redução das porcentagens dos monócitos CD16+ após o tratamento. A produção de TNF e a de IL-10 não estavam significantemente alteradas nas hemoculturas de pacientes, quando comparadas com as de controles sadios. Entre os estímulos usados, somente o AG não induziu quantidades significantes de IL-10 nas hemoculturas dos pacientes. Após o tratamento, as concentrações de TNF diminuíram nas hemoculturas de pacientes com LC, exceto quando o estímulo foi Pam3Cys (TLR2), o qual induziu aumento na produção de TNF (p < 0,05). Em hemoculturas de pacientes com LM não foram detectadas diferenças significantes entre as concentrações de TNF e IL-10 produzidas antes e após o tratamento. As concentrações de IL-10 também não foram significantemente alteradas após o tratamento dos pacientes com LC. Os dados indicam que as porcentagens de monócitos CD16+ estão aumentadas na LC. Ainda, sugerem que monócitos de pacientes com LC ou LM apresentam uma diminuída capacidade de produção de IL-10 em resposta ao AG, o que pode dificultar o controle da resposta inflamatória. Os dados também sugerem que a capacidade de resposta dos monócitos via TLR2 pode estar suprimida na LC, sendo elevada após o tratamento. Uma análise de TLR2 e TLR4 em monócitos de pacientes com LTA pode auxiliar a entender melhor a indução de citocinas nestas células.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-01T11:58:23Z No. of bitstreams: 2 Dissertação - Poliana Ribeiro Valadares Veras - 2015.pdf: 4546054 bytes, checksum: d4bed497af887b82ba08b0d21d58ed42 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-01T11:58:38Z (GMT) No. of bitstreams: 2 Dissertação - Poliana Ribeiro Valadares Veras - 2015.pdf: 4546054 bytes, checksum: d4bed497af887b82ba08b0d21d58ed42 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-09-01T11:58:38Z (GMT). No. of bitstreams: 2 Dissertação - Poliana Ribeiro Valadares Veras - 2015.pdf: 4546054 bytes, checksum: d4bed497af887b82ba08b0d21d58ed42 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-07-02application/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessLeishmaniosesMonócitosLeishmaniasisMonocytesIMUNOLOGIA::IMUNOLOGIA APLICADAAvaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamentoEvaluation of monocytes from patients with tegumentary leishmaniasis before and after treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6085308344741430434600600600-7769011444564556288-7923306237058907834reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
dc.title.alternative.eng.fl_str_mv Evaluation of monocytes from patients with tegumentary leishmaniasis before and after treatment
title Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
spellingShingle Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
Veras, Poliana Ribeiro Valadares
Leishmanioses
Monócitos
Leishmaniasis
Monocytes
IMUNOLOGIA::IMUNOLOGIA APLICADA
title_short Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
title_full Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
title_fullStr Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
title_full_unstemmed Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
title_sort Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento
author Veras, Poliana Ribeiro Valadares
author_facet Veras, Poliana Ribeiro Valadares
author_role author
dc.contributor.advisor1.fl_str_mv Dias, Fátima Ribeiro
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5741031258926403
dc.contributor.referee1.fl_str_mv Dorta, Miriam Leandro
dc.contributor.referee2.fl_str_mv Gomes, Rodrigo Saar
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3032438721919576
dc.contributor.author.fl_str_mv Veras, Poliana Ribeiro Valadares
contributor_str_mv Dias, Fátima Ribeiro
Dorta, Miriam Leandro
Gomes, Rodrigo Saar
dc.subject.por.fl_str_mv Leishmanioses
Monócitos
topic Leishmanioses
Monócitos
Leishmaniasis
Monocytes
IMUNOLOGIA::IMUNOLOGIA APLICADA
dc.subject.eng.fl_str_mv Leishmaniasis
Monocytes
dc.subject.cnpq.fl_str_mv IMUNOLOGIA::IMUNOLOGIA APLICADA
description American tegumentary leishmaniasis (ATL) is an infectious parasitic disease caused by Leishmania protozoa. The disease presents as cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML). The mechanisms of the immunopathogenesis and infection control are not yet fully understood, and monocytes can be involved in these mechanisms. The monocytes are divided into three subsets (CD14hiCD16-, classical monocytes; CD14hiCD16+, the intermediates; and CD14loCD16+ non- classical) and are the major cell producing cytokines in peripheral blood.The objective of this study was to assess possible alterations in the percentages of monocytes subsets; and in the tumor necrosis factor (TNF) and interleukin 10 (IL -10) production in whole blood cultures from patients with CL and ML, before and after treatment. Peripheral blood from patients (n = 54; 31 CL and ML 23) and controls (n = 54) was used to identify monocytes by flow cytometry and for whole blood cultures. The blood was incubated in the absence (medium) or presence of toll-like receptor agonists (Pam3Cys and LPS for TLR2 and TLR4, respectively) or L. (V.) braziliensisantigens (AG) to assess TNF and IL-10. Cytokines were quantified by enzyme-linked immunosorbent assay (ELISA).The results showed an increase in the percentage of CD16+ monocytes, especially CD14loCD16+monocyte subset in CL patients, but not in ML, before treatment (p <0.05). After treatment, the percentages of these monocytes in CL patients back to similar levels of those from healthy individuals. In patients with ML, there was also a reduction in the percentages of CD16+ monocytes after treatment. The production of TNF and IL-10 was not significantly altered in whole blood cultures from patients, compared with those from healthy controls. Among the stimuli used, only the AG did not induce significant amounts of IL-10 in whole blood cultures from patients. After treatment, TNF concentrations decreased in CL whole blood cultures, except when the stimulus was Pam3Cys (TLR2), which induced an increase in TNF levels (p <0.05). In ML whole blood cultures no significant differences were detected between the concentrations of TNF and IL-10 produced before and after treatment.The IL-10 concentrations were not significantly altered after treatment of CL patients. The data indicate that percentages of CD16+ monocytes are increased in CL. Also, they suggest that monocytes from patients with CL or ML show a decreased capacity to produce IL-10 in response to AG, what can hamper the control of the inflammatory response. The data also suggest that the ability of monocytes to be activatedthrough TLR2 can be suppressed in CL what isrecovered after treatment. An analysis of TLR2 and TLR4 in monocytes from patients with ATL can improve the knowledge about cytokine induction in these cells.
publishDate 2015
dc.date.issued.fl_str_mv 2015-07-02
dc.date.accessioned.fl_str_mv 2016-09-01T11:58:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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dc.identifier.citation.fl_str_mv VERAS, P. R. V. Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento. 2015. 56 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/6069
dc.identifier.dark.fl_str_mv ark:/38995/0013000002zpz
identifier_str_mv VERAS, P. R. V. Avaliação dos monócitos de pacientes com Leishmaniose Tegumentar Americana antes e após o tratamento. 2015. 56 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.
ark:/38995/0013000002zpz
url http://repositorio.bc.ufg.br/tede/handle/tede/6069
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language por
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dc.relation.confidence.fl_str_mv 600
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dc.relation.cnpq.fl_str_mv -7923306237058907834
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
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instname_str Universidade Federal de Goiás (UFG)
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institution UFG
reponame_str Repositório Institucional da UFG
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repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
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