Prevalência, fatores de risco e genótipos do vírus da hepatite C em usuários de crack institucionalizados em Goiânia, Goiás

• Autor(a) principal: Del-Rios, Nativa Helena Alves
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8059
• topic: Hepatite C; Usuários de crack; Genótipos; Fatores de risco; Hepatitis C; Crack users; Genotypes; Risk factors; CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Crack users are uniquely vulnerable to acquisition and transmission of infectious agents such as hepatitis C virus (HCV) because the artifacts used for drug consumption are often shared among individuals with lesions in the oral cavity. The objective of this study was to investigate the epidemiological and molecular profile of infection with hepatitis C virus among in-treatment crack users in Goiania, Goias, Central Brazil. The population consisted of 600 crack users in a drug treatment hospital in Goiania (Spiritist Hospital Euripides Barsanulfo - Euripides House), from August 2012 to April 2013. Blood samples were tested for antibodies to HCV by (Enzyme-Linked Immunosorbent Assay - ELISA). Samples with anti-HCV reagents were subjected to detection of viral RNA by RT-PCR (Reverse Transcription - Polymerase Chain Reaction). All HCV RNA-positive samples were genotyped by line probe assay - LiPA, using the 5 'NC and direct sequencing of the NS5B region of the viral genome, followed by phylogenetic analysis. The prevalence of HCV infection among the study participants was 3.7% (95% CI: 2.4% -5.6%). Viral RNA was detected in 63.6% of the samples for the 5 'NC region and 54.5% in the NS5B region. Genotypes 1 and 3 were identified, predominantly subtype 1a (n = 7) followed subtype 1b (n = 3) and 3a (n = 2). Following logistic regression, age older than 40 years (OR: 17.72; 95% CI: 6.22 to 50.48) and a history of injection drug use (OR: 11.10; 95% CI: 3.95 to 31, 22) were associated with HCV infection (p <0.05). These findings reinforce the need to implement public health policies emphasizing prevention and control of hepatitis C, aimed specifically toward crack users.