Detecção molecular de Salmonella sp. em amostras avícolas

• Autor(a) principal: Medeiros, Nadielly Xavier de
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/5217
• topic: Diagnóstico; Frangos; PCR em tempo real; Salmonella sp; Broiler; Diagnostic; Real-time PCR; Salmonella sp; CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS

Salmonella is recognized as an etiological agent of food poisoning and it is classified as one of the most relevant microorganisms to public health, as well as to the monitoring of poultry industry. Salmonella is widely spread in environment and has asymptomatic transmitters, which also favors its spread during food processing steps, becoming a considerable problem for the processing industry. Monitoring pathogens in poultry slaughterhouses demands the application of fast and reliable methodologies. In order to investigate the presence of Salmonella in slaughterhouses, this study aimed to evaluate swabs of picking machines and evisceration flume, carcasses, hearts, livers, and gizzards of poultry. Laboratory tests were developed in the Laboratory of Bacteriology and Molecular Biology, Department of Veterinary Preventive Medicine, Medicine Veterinary College and Animal Science of the Universidade Federal de Goiás (Federal University of Goiás), and circumscribed to the laboratory research of pre-enrichment broths composed of peptone water to 1%, added to the samples. Therefore, after inoculation of broth samples were incubated for a period of 18 to 24 hours, and then frozen at -18 °C for a later analyzes. The analytical methods of choice for detecting Salmonella sp. were conventional polymerase chain reaction (PCR) and real-time PCR, there had been also check for previous studies with conventional bacterial isolation and VIDAS - SLM. Target genes were, respectively, iroB and bipA, associated to bacterial virulence and its adaptation face to stressful situations. Gizzard was the sample category with the largest contamination percentage (13.3%). The real-time PCR technique was able to identify a greater number of positive samples, 22 (7.3%) compared to 5 (1.6%) by conventional PCR; however, there was no equivalent to the most previous results of total bacterial isolation. In conclusion, Real-time PCR can expedite the laboratory diagnosis, being made selective enrichment with immediately pre-application of the culture method, and salmonella is present in poultry source food, in installations, and equipment.