Detecção molecular de Salmonella sp. em amostras avícolas
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/5217 |
Resumo: | Salmonella is recognized as an etiological agent of food poisoning and it is classified as one of the most relevant microorganisms to public health, as well as to the monitoring of poultry industry. Salmonella is widely spread in environment and has asymptomatic transmitters, which also favors its spread during food processing steps, becoming a considerable problem for the processing industry. Monitoring pathogens in poultry slaughterhouses demands the application of fast and reliable methodologies. In order to investigate the presence of Salmonella in slaughterhouses, this study aimed to evaluate swabs of picking machines and evisceration flume, carcasses, hearts, livers, and gizzards of poultry. Laboratory tests were developed in the Laboratory of Bacteriology and Molecular Biology, Department of Veterinary Preventive Medicine, Medicine Veterinary College and Animal Science of the Universidade Federal de Goiás (Federal University of Goiás), and circumscribed to the laboratory research of pre-enrichment broths composed of peptone water to 1%, added to the samples. Therefore, after inoculation of broth samples were incubated for a period of 18 to 24 hours, and then frozen at -18 °C for a later analyzes. The analytical methods of choice for detecting Salmonella sp. were conventional polymerase chain reaction (PCR) and real-time PCR, there had been also check for previous studies with conventional bacterial isolation and VIDAS - SLM. Target genes were, respectively, iroB and bipA, associated to bacterial virulence and its adaptation face to stressful situations. Gizzard was the sample category with the largest contamination percentage (13.3%). The real-time PCR technique was able to identify a greater number of positive samples, 22 (7.3%) compared to 5 (1.6%) by conventional PCR; however, there was no equivalent to the most previous results of total bacterial isolation. In conclusion, Real-time PCR can expedite the laboratory diagnosis, being made selective enrichment with immediately pre-application of the culture method, and salmonella is present in poultry source food, in installations, and equipment. |
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Rezende, Cíntia Silva Minafra ehttp://lattes.cnpq.br/5841210447886226Prado, Cristiano Saleshttp://lattes.cnpq.br/1661902818272650Rezende, Cíntia Silva Minafra eDuarte, Sabrina CastilhoAndrade, Maria Auxiliadora dehttp://lattes.cnpq.br/1086357508138344Medeiros, Nadielly Xavier de2016-02-11T11:08:43Z2013-06-21MEDEIROS, N. X. Detecção molecular de Salmonella sp. em amostras avícolas. 2013. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013.http://repositorio.bc.ufg.br/tede/handle/tede/5217ark:/38995/0013000006gbrSalmonella is recognized as an etiological agent of food poisoning and it is classified as one of the most relevant microorganisms to public health, as well as to the monitoring of poultry industry. Salmonella is widely spread in environment and has asymptomatic transmitters, which also favors its spread during food processing steps, becoming a considerable problem for the processing industry. Monitoring pathogens in poultry slaughterhouses demands the application of fast and reliable methodologies. In order to investigate the presence of Salmonella in slaughterhouses, this study aimed to evaluate swabs of picking machines and evisceration flume, carcasses, hearts, livers, and gizzards of poultry. Laboratory tests were developed in the Laboratory of Bacteriology and Molecular Biology, Department of Veterinary Preventive Medicine, Medicine Veterinary College and Animal Science of the Universidade Federal de Goiás (Federal University of Goiás), and circumscribed to the laboratory research of pre-enrichment broths composed of peptone water to 1%, added to the samples. Therefore, after inoculation of broth samples were incubated for a period of 18 to 24 hours, and then frozen at -18 °C for a later analyzes. The analytical methods of choice for detecting Salmonella sp. were conventional polymerase chain reaction (PCR) and real-time PCR, there had been also check for previous studies with conventional bacterial isolation and VIDAS - SLM. Target genes were, respectively, iroB and bipA, associated to bacterial virulence and its adaptation face to stressful situations. Gizzard was the sample category with the largest contamination percentage (13.3%). The real-time PCR technique was able to identify a greater number of positive samples, 22 (7.3%) compared to 5 (1.6%) by conventional PCR; however, there was no equivalent to the most previous results of total bacterial isolation. In conclusion, Real-time PCR can expedite the laboratory diagnosis, being made selective enrichment with immediately pre-application of the culture method, and salmonella is present in poultry source food, in installations, and equipment.Salmonella é reconhecida como agente etiológico de intoxicações alimentares e classifica-se como um dos microrganismos de maior relevância à saúde pública, bem como ao monitoramento dos plantéis avícolas. Esta bactéria está amplamente distribuída no meio ambiente, e possui portadores assintomáticos, o que, também, favorece sua disseminação durante etapas do processamento de alimentos, transformando-se em grande problema para as agroindústrias. O monitoramento de patógenos em abatedouros de aves demanda a aplicação de metodologias rápidas e confiáveis. Com o intuito de investigar a presença de Salmonella em abatedouros, objetivou-se avaliar suabes de depenadeiras e de calhas de evisceração, carcaças, coração, fígado e moela de aves. As análises laboratoriais foram desenvolvidas no Laboratório de Bacteriologia e Biologia Molecular, do Setor de Medicina Veterinária Preventiva da Escola de Veterinária e Zootecnia da Universidade Federal de Goiás e circunscreveram-se à investigação laboratorial de caldos de pré-enriquecimento compostos por água peptonada a 1%, adicionados das amostras. Para tanto, após a inoculação das amostras os caldos foram incubados pelo período de 18 a 24 horas e em seguida congelados a temperatura de -18°C, para posteriormente, serem analisados. Os métodos analíticos de eleição, para detecção de Salmonella sp., foram a reação em cadeia pela polimerase (PCR) convencional e PCR em tempo real, havendo também verificação para estudos prévios com isolamento bacteriano convencional e VIDAS-SLM. Os genes alvo foram, respectivamente, iroB e bipA, associados à virulência da bactéria e sua adaptação frente às situações de estresse. Moela foi a categoria de amostra de maior percentual de contaminação (13,3%). Verificou-se que a técnica de PCR em tempo real foi capaz de identificar maior número de amostras positivas, 22 (7,3%) em comparação à PCR convencional que detectou cinco (1,6%), no entanto, não houve equivalência à maioria dos resultados prévios do isolamento bacteriano total. Conclui-se que a PCR em tempo real pode agilizar o diagnóstico laboratorial, sendo feito pré-enriquecimento seletivo com aplicação do meio de cultura de forma imediata e que Salmonella está presente em alimentos de origem avícola e em instalações e equipamentos.Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2016-02-11T11:04:21Z No. of bitstreams: 2 Dissertação - Nadielly Xavier de Medeiros - 2013.pdf: 1507098 bytes, checksum: 3e76159239ff4a156048bd0d749eb1ac (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-02-11T11:08:43Z (GMT) No. of bitstreams: 2 Dissertação - Nadielly Xavier de Medeiros - 2013.pdf: 1507098 bytes, checksum: 3e76159239ff4a156048bd0d749eb1ac (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2016-02-11T11:08:43Z (GMT). No. of bitstreams: 2 Dissertação - Nadielly Xavier de Medeiros - 2013.pdf: 1507098 bytes, checksum: 3e76159239ff4a156048bd0d749eb1ac (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-06-21Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessDiagnósticoFrangosPCR em tempo realSalmonella spBroilerDiagnosticReal-time PCRSalmonella spCIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOSDetecção molecular de Salmonella sp. em amostras avícolasMolecular detection of Salmonella sp. in poultry samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis4581960685150189167600600600600-6217552114249094582-76365128114794953382075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Detecção molecular de Salmonella sp. em amostras avícolas |
dc.title.alternative.eng.fl_str_mv |
Molecular detection of Salmonella sp. in poultry samples |
title |
Detecção molecular de Salmonella sp. em amostras avícolas |
spellingShingle |
Detecção molecular de Salmonella sp. em amostras avícolas Medeiros, Nadielly Xavier de Diagnóstico Frangos PCR em tempo real Salmonella sp Broiler Diagnostic Real-time PCR Salmonella sp CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
title_short |
Detecção molecular de Salmonella sp. em amostras avícolas |
title_full |
Detecção molecular de Salmonella sp. em amostras avícolas |
title_fullStr |
Detecção molecular de Salmonella sp. em amostras avícolas |
title_full_unstemmed |
Detecção molecular de Salmonella sp. em amostras avícolas |
title_sort |
Detecção molecular de Salmonella sp. em amostras avícolas |
author |
Medeiros, Nadielly Xavier de |
author_facet |
Medeiros, Nadielly Xavier de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rezende, Cíntia Silva Minafra e |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5841210447886226 |
dc.contributor.advisor-co1.fl_str_mv |
Prado, Cristiano Sales |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1661902818272650 |
dc.contributor.referee1.fl_str_mv |
Rezende, Cíntia Silva Minafra e |
dc.contributor.referee2.fl_str_mv |
Duarte, Sabrina Castilho |
dc.contributor.referee3.fl_str_mv |
Andrade, Maria Auxiliadora de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1086357508138344 |
dc.contributor.author.fl_str_mv |
Medeiros, Nadielly Xavier de |
contributor_str_mv |
Rezende, Cíntia Silva Minafra e Prado, Cristiano Sales Rezende, Cíntia Silva Minafra e Duarte, Sabrina Castilho Andrade, Maria Auxiliadora de |
dc.subject.por.fl_str_mv |
Diagnóstico Frangos PCR em tempo real Salmonella sp |
topic |
Diagnóstico Frangos PCR em tempo real Salmonella sp Broiler Diagnostic Real-time PCR Salmonella sp CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Broiler Diagnostic Real-time PCR Salmonella sp |
dc.subject.cnpq.fl_str_mv |
CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
description |
Salmonella is recognized as an etiological agent of food poisoning and it is classified as one of the most relevant microorganisms to public health, as well as to the monitoring of poultry industry. Salmonella is widely spread in environment and has asymptomatic transmitters, which also favors its spread during food processing steps, becoming a considerable problem for the processing industry. Monitoring pathogens in poultry slaughterhouses demands the application of fast and reliable methodologies. In order to investigate the presence of Salmonella in slaughterhouses, this study aimed to evaluate swabs of picking machines and evisceration flume, carcasses, hearts, livers, and gizzards of poultry. Laboratory tests were developed in the Laboratory of Bacteriology and Molecular Biology, Department of Veterinary Preventive Medicine, Medicine Veterinary College and Animal Science of the Universidade Federal de Goiás (Federal University of Goiás), and circumscribed to the laboratory research of pre-enrichment broths composed of peptone water to 1%, added to the samples. Therefore, after inoculation of broth samples were incubated for a period of 18 to 24 hours, and then frozen at -18 °C for a later analyzes. The analytical methods of choice for detecting Salmonella sp. were conventional polymerase chain reaction (PCR) and real-time PCR, there had been also check for previous studies with conventional bacterial isolation and VIDAS - SLM. Target genes were, respectively, iroB and bipA, associated to bacterial virulence and its adaptation face to stressful situations. Gizzard was the sample category with the largest contamination percentage (13.3%). The real-time PCR technique was able to identify a greater number of positive samples, 22 (7.3%) compared to 5 (1.6%) by conventional PCR; however, there was no equivalent to the most previous results of total bacterial isolation. In conclusion, Real-time PCR can expedite the laboratory diagnosis, being made selective enrichment with immediately pre-application of the culture method, and salmonella is present in poultry source food, in installations, and equipment. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-06-21 |
dc.date.accessioned.fl_str_mv |
2016-02-11T11:08:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MEDEIROS, N. X. Detecção molecular de Salmonella sp. em amostras avícolas. 2013. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/5217 |
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ark:/38995/0013000006gbr |
identifier_str_mv |
MEDEIROS, N. X. Detecção molecular de Salmonella sp. em amostras avícolas. 2013. 51 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013. ark:/38995/0013000006gbr |
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http://repositorio.bc.ufg.br/tede/handle/tede/5217 |
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por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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