Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/10700 |
Resumo: | The rural environment lacks basic sanitation services. Thus, the water supply and sewage disposal facilities are often under the initiative of each resident, who may not have the financial resources and technical knowledge to build and keep them functioning properly. Thus, water for human consumption is subject to fecal contamination and, consequently, to the presence of waterborne pathogens, such as enteric viruses. The objective of this work was to evaluate the fecal contamination of water samples from individual sources used for household supply in rural areas of the State of Goiás. The samples were collected from 86 homes, distributed in 15 communities, whose water sources were tubular wells, dug wells, springs, surface waters and rainwater. Escherichia coli (EC) bacteria, analyzed by the defined chromogenic substrate method, and the enteric virus human adenovirus (HAdV) and enterovirus (EV), analyzed by qPCR, were used as fecal contamination indicators. It was observed that 90.7% of the samples showed indications of fecal contamination. Detection rates were 74.4% for EC, 57% for HAdV and 9.3% for EV. The concentration averages of these indicators were, respectively, 8.34 x 101 NMP / 100mL, 8.6 x 105 CG / L and 9.75 x 105 CG / L. The EC indicator was the most prevalent in ground and surface water samples. The HAdV indicator was significantly more detected in groundwater samples than in surface water and was more efficient in indicating contamination in tubular wells. In cistern samples, viral indicators were the most prevalent. There was no association of frequencies or correlation of concentrations between EC and HAdV. HAdV indicated human fecal contamination and performed well as a complementary indicator. These results reveal that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens. |
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Carneiro, Lilian Carlahttp://lattes.cnpq.br/6506744224041777Scalize, Paulo SérgioSantos, Mônica de OliveiraGabriel, Ellen Flávia MoreiraGama, Aline Rodrigueshttp://lattes.cnpq.br/9162511540634652Lima, Fernando Santos2020-09-15T11:29:15Z2020-09-15T11:29:15Z2020-06-23LIMA, F. S. Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás. 2020. 62 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10700The rural environment lacks basic sanitation services. Thus, the water supply and sewage disposal facilities are often under the initiative of each resident, who may not have the financial resources and technical knowledge to build and keep them functioning properly. Thus, water for human consumption is subject to fecal contamination and, consequently, to the presence of waterborne pathogens, such as enteric viruses. The objective of this work was to evaluate the fecal contamination of water samples from individual sources used for household supply in rural areas of the State of Goiás. The samples were collected from 86 homes, distributed in 15 communities, whose water sources were tubular wells, dug wells, springs, surface waters and rainwater. Escherichia coli (EC) bacteria, analyzed by the defined chromogenic substrate method, and the enteric virus human adenovirus (HAdV) and enterovirus (EV), analyzed by qPCR, were used as fecal contamination indicators. It was observed that 90.7% of the samples showed indications of fecal contamination. Detection rates were 74.4% for EC, 57% for HAdV and 9.3% for EV. The concentration averages of these indicators were, respectively, 8.34 x 101 NMP / 100mL, 8.6 x 105 CG / L and 9.75 x 105 CG / L. The EC indicator was the most prevalent in ground and surface water samples. The HAdV indicator was significantly more detected in groundwater samples than in surface water and was more efficient in indicating contamination in tubular wells. In cistern samples, viral indicators were the most prevalent. There was no association of frequencies or correlation of concentrations between EC and HAdV. HAdV indicated human fecal contamination and performed well as a complementary indicator. These results reveal that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens.O meio rural carece de serviços de saneamento básico. Assim, frequentemente, as instalações de obtenção de água e de disposição de esgoto ficam sob iniciativa de cada morador, os quais podem não contar com o recurso financeiro e o conhecimento técnico para construí-las e mantê-las funcionando adequadamente. Dessa forma, a água para consumo humano fica sujeita à contaminação fecal e, consequentemente, à presença de patógenos de veiculação hídrica, como os vírus entéricos. O objetivo desse trabalho foi avaliar a contaminação fecal de amostras de água proveniente de fontes individuais utilizadas para o abastecimento domiciliar em áreas rurais do Estado de Goiás. As amostras foram coletadas de 86 residências, distribuídas em 15 comunidades, cujas fontes de água eram poços tubulares, poços escavados, nascentes, mananciais superficiais e água da chuva. Utilizou-se como indicadores de contaminação fecal a bactéria Escherichia coli (EC), analisada pelo método do substrato cromogênico definido, e os vírus entéricos adenovírus humano (HAdV) e enterovírus (EV), analisados por qPCR. Observou-se que 90,7% das amostras apresentaram indicativo de contaminação fecal. As taxas de detecção foram 74,4% para EC, 57% para HAdV e 9,3% para EV. As médias de concentração desses indicadores foram, respectivamente, 8,34 x 101 NMP/100mL, 8,6 x 105 CG/L e 9,75 x 105 CG/L. O indicador EC foi o mais prevalente em amostras de água subterrânea e superficial. O indicador HAdV foi significativamente mais detectado em amostras de águas subterrâneas em relação as de águas superficiais e foi mais eficiente em indicar contaminação em poços tubulares. Em amostras de cisternas, os indicadores virais foram os mais prevalentes. Não foi observada associação de frequências nem correlação de concentrações entre EC e HAdV. HAdV indicou contaminação fecal humana e teve bom desempenho como indicador complementar. Esses resultados revelam que a população analisada está vulnerável a doenças de veiculação hídrica causadas por patógenos entéricos.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-14T14:11:26Z No. of bitstreams: 2 Dissertação - Fernando Santos Lima - 2020.pdf: 1051764 bytes, checksum: 777cef94e105abd7a64abbf59840a76a (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-15T11:29:15Z (GMT) No. of bitstreams: 2 Dissertação - Fernando Santos Lima - 2020.pdf: 1051764 bytes, checksum: 777cef94e105abd7a64abbf59840a76a (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5)Made available in DSpace on 2020-09-15T11:29:15Z (GMT). No. of bitstreams: 2 Dissertação - Fernando Santos Lima - 2020.pdf: 1051764 bytes, checksum: 777cef94e105abd7a64abbf59840a76a (MD5) license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Previous issue date: 2020-06-23Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessComunidades ruraisFontes de águaQualidade da águaContaminação fecalVírus entéricosRural communitiesWater sourcesWater qualityFecal contaminationEnteric virusesCIENCIAS DA SAUDE::SAUDE COLETIVAEscherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de GoiásEscherichia coli, adenovirus and enterovirus in samples of water consumed in rural areas of Goiásinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis15500500500500291811reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Fernando Santos Lima - 2020.pdfDissertação - Fernando Santos Lima - 2020.pdfapplication/pdf1051764http://repositorio.bc.ufg.br/tede/bitstreams/5926b390-8f4d-42fc-9796-5a4a37f4e0ac/download777cef94e105abd7a64abbf59840a76aMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/633878a8-02dd-4906-bf96-7963ae3f8bd6/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/436e43d8-aa37-4454-bacf-85d2ec150296/downloade39d27027a6cc9cb039ad269a5db8e34MD52tede/107002020-09-15 08:29:15.808http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10700http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-09-15T11:29:15Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)falseTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |
dc.title.pt_BR.fl_str_mv |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
dc.title.alternative.eng.fl_str_mv |
Escherichia coli, adenovirus and enterovirus in samples of water consumed in rural areas of Goiás |
title |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
spellingShingle |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás Lima, Fernando Santos Comunidades rurais Fontes de água Qualidade da água Contaminação fecal Vírus entéricos Rural communities Water sources Water quality Fecal contamination Enteric viruses CIENCIAS DA SAUDE::SAUDE COLETIVA |
title_short |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
title_full |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
title_fullStr |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
title_full_unstemmed |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
title_sort |
Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás |
author |
Lima, Fernando Santos |
author_facet |
Lima, Fernando Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Carneiro, Lilian Carla |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6506744224041777 |
dc.contributor.referee1.fl_str_mv |
Scalize, Paulo Sérgio |
dc.contributor.referee2.fl_str_mv |
Santos, Mônica de Oliveira |
dc.contributor.referee3.fl_str_mv |
Gabriel, Ellen Flávia Moreira |
dc.contributor.referee4.fl_str_mv |
Gama, Aline Rodrigues |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9162511540634652 |
dc.contributor.author.fl_str_mv |
Lima, Fernando Santos |
contributor_str_mv |
Carneiro, Lilian Carla Scalize, Paulo Sérgio Santos, Mônica de Oliveira Gabriel, Ellen Flávia Moreira Gama, Aline Rodrigues |
dc.subject.por.fl_str_mv |
Comunidades rurais Fontes de água Qualidade da água Contaminação fecal Vírus entéricos |
topic |
Comunidades rurais Fontes de água Qualidade da água Contaminação fecal Vírus entéricos Rural communities Water sources Water quality Fecal contamination Enteric viruses CIENCIAS DA SAUDE::SAUDE COLETIVA |
dc.subject.eng.fl_str_mv |
Rural communities Water sources Water quality Fecal contamination Enteric viruses |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::SAUDE COLETIVA |
description |
The rural environment lacks basic sanitation services. Thus, the water supply and sewage disposal facilities are often under the initiative of each resident, who may not have the financial resources and technical knowledge to build and keep them functioning properly. Thus, water for human consumption is subject to fecal contamination and, consequently, to the presence of waterborne pathogens, such as enteric viruses. The objective of this work was to evaluate the fecal contamination of water samples from individual sources used for household supply in rural areas of the State of Goiás. The samples were collected from 86 homes, distributed in 15 communities, whose water sources were tubular wells, dug wells, springs, surface waters and rainwater. Escherichia coli (EC) bacteria, analyzed by the defined chromogenic substrate method, and the enteric virus human adenovirus (HAdV) and enterovirus (EV), analyzed by qPCR, were used as fecal contamination indicators. It was observed that 90.7% of the samples showed indications of fecal contamination. Detection rates were 74.4% for EC, 57% for HAdV and 9.3% for EV. The concentration averages of these indicators were, respectively, 8.34 x 101 NMP / 100mL, 8.6 x 105 CG / L and 9.75 x 105 CG / L. The EC indicator was the most prevalent in ground and surface water samples. The HAdV indicator was significantly more detected in groundwater samples than in surface water and was more efficient in indicating contamination in tubular wells. In cistern samples, viral indicators were the most prevalent. There was no association of frequencies or correlation of concentrations between EC and HAdV. HAdV indicated human fecal contamination and performed well as a complementary indicator. These results reveal that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-15T11:29:15Z |
dc.date.available.fl_str_mv |
2020-09-15T11:29:15Z |
dc.date.issued.fl_str_mv |
2020-06-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, F. S. Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás. 2020. 62 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10700 |
identifier_str_mv |
LIMA, F. S. Escherichia coli, adenovírus e enterovírus em amostras de água consumida em áreas rurais de Goiás. 2020. 62 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2020. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10700 |
dc.language.iso.fl_str_mv |
por |
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por |
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15 |
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500 500 500 500 |
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181 |
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1 |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP) |
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UFG |
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Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
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Universidade Federal de Goiás |
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