Desenvolvimento de sensores colorimétricos em materiais poliméricos para detecção de biomarcadores para diagnósticos clínicos

• Autor(a) principal: Oliveira, Karoliny Almeida
• Data de Publicação: 2017
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/001300000fbrq
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8652
• topic: Microzonas impressa; Bioensaios; Detecção colorimétrica; Análise multivariada de imagem; Printed microzones; Bioassays; Colorimetric detection; Multivariate image analysis; CIENCIAS EXATAS E DA TERRA::QUIMICA

This thesis describes the development of colorimetric sensors in polymeric materials for the biomarkers detection for clinical diagnostics. The devices were manufactured with 96 microzones from the direct printing technology, using transparency film. The printed microplates were used to detect dengue, glucose and total proteins. Dengue detection in patient serum samples was performed with the capture and indirect ELISA assay for the detection of immunoglobulin M and immunoglobulin G, respectively. After the acquisition of the images with a cellular camera, a color histogram analysis and the principal component analysis (PCA) were performed. The results of these analyzes allowed to easily differentiate the groups of serum samples from patients who were infected and non-infected with the dengue virus. In order to promote carboxyl groups on the surface of the polyester film, this was modified by two methodologies: alkaline hydrolysis and adsorption of a polymer. The glucose assay was performed on the films modified by both methodologies and detected by a cellular camera, and the detection limits found were 0.70 mM and 0.93 mM for the polymer and hydrolyzed film, respectively. In addition, the biuret assay was performed on the microplates for the analysis of total proteins in canine serum samples using a bench scanner as the detection system. The limit of detection was 9.7 μM for the analytical curve ranging from 0 to 200 μM albumin.