Potencial biotecnológico de linhagens bacterianas endofíticas de Anacardium othonianum Rizzini

• Autor(a) principal: Faria, Paula Sperotto Alberto
• Data de Publicação: 2017
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/0013000004qtf
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9284
• topic: Caju-de-árvore-do-cerrado; Ecologia microbiana; Biotecnologia; Traços funcionais; Promoção de crescimento vegetal; Cerrado-tree-cashew; Microbial ecology; Biotechnology; Functional traits; Promotion of plant growth; CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Plants are filled with microorganisms that colonize their structures, those capable of inhabiting plant tissues internally are known as endophytes and provide new nutritional and defense pathways, acting as a plant strategy to withstand biotic and abiotic adversities. Anacardium othonianum Rizzini, also known as cerrado-tree-cashew, is one of the main cashew trees of economic and social importance in the central-western region of Brazil, but its endophytic bacterial community has not been investigated yet. In this regard, the aim of this work was to define endophytic bacterial lineages with functional potential to promote growth of Anacardium othonianum Rizzini. Root fragments of A. othonianum were inoculated in a culture medium. Grown colonies were isolated, purified and the identification was performed by comparing the 16S rDNA sequence in the GenBank database. Subsequently, in vitro tests of biostimulation, biofertilization and biocontrol were performed to select lineages with multifactors that promote plant growth. Two endophytic lines were selected and investigated with in vivo inoculation in A. othonianum to confirm their growth promoting action. This work is the first report on the isolation, selection and application of endophytic bacteria that promote plant growth of A. othonianum. Twenty-two species were isolated, of which Acinetobacter sp. Bac109, Bacillus mycoides Bac160, Lysinibacillus sphaericus Bac161 and Pantoea agglomerans Bac131 with multifactors of interest. Co-inoculation of A. othonianum with the endophytic bacteria Acinetobacter sp. (Bac109) and Pantoea agglomerans (Bac131), promoted an increase in initial growth.