Efeitos cardiometabólicos e renais de peptídeos bioativos do feijão comum (phaseolus vulgaris)

• Autor(a) principal: Ribeiro, Juliana Vila Verde
• Data de Publicação: 2022
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/001300000c15s
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/13069
• topic: Feijão comum; Feijão endurecido; Peptídeos bioativos; PV3; hipertensão; Resistência à insulina e obesidade; Common beans; Hardened beans; Bioactive peptides; Hypertension; Insulin resistance and obesity; CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA GERAL

The common bean is susceptible to the hardening process and once hardened, it is not commercialized, being considered an agroindustry residue. However, it is possible to use it as a source of proteins and bioactive peptides. The bean bioactive peptides may have antihypertensive, antihyperglycemic, hypoglycemic, antioxidant and hypocholesterolemic activity. Thus, this work sought to study the bioactivity of the low molecular weight peptide fraction extracted from hardened common bean (Phaseolus vulgaris) grains on renal, cardiovascular and metabolic functions. To evaluate the effect of the common bean peptide, the following methodologies were used: I) Obtaining the extract and peptide fraction – from the hardened beans, flour was made using a knife mill. This flour was used to extract the peptides and proteins, using a combined solution of acetonitrile, water and formic acid (25: 24: 1), this extract was subjected to the fractionation process, using a 3 kDa filtration membrane, this extract was named as PV3. II) Comparison of PV3 amino acid sequences with other amino acid sequences reported by other authors. III) Evaluation of osmolarity and sodium and potassium ions in the PV3 extract. IV) Evaluation of the vasorelaxant effect and physiological mechanisms involved in vasorelaxation in renal artery rings from normotensive (Wistar) and hypertensive (SHR) animals. V) Evaluation of renal parameters in WT and SHR animals. VI) Evaluation of hemodynamic parameters in WT and SHR. VII) Evaluation of body mass gains and food consumption in C57BL6/6J mice submitted to a high-fat diet and administration of PV3 by pseudogavem. VIII) Assessment of glycemic levels, pyruvate, insulin and pyruvate tolerance test. IX) Evaluation of the mechanisms involved in PV3 in the insulin pathway by Western Blotting and qPCR. X) evaluation of the energy metabolism of PV3 by q PCR. The main results of this study are: I) homology of amino acid sequences of PV3 with several amino acid sequences described in the literature that show antihypertensive activity, II) PV3 causes a relaxing effect dependent on the endothelium and oxidonitrergic pathways; III) PV3 caused a natriuretic effect and decreased the glomerular filtration rate; IV) PV3 caused a hypotensive effect that was concomitant to a reduction in vascular resistance in the aortic and renal vascular beds; V) PV3 prevented the damage caused by the hyperlipidic diet, such as reduction of adiposity and glycemic levels.; VI) PV3 acts on the IRS/PI3K and AMPK pathway to reduce glycemic levels; 22 VII) PV3 acts in the regulation of energy balance. Such findings are relevant for the molecular and physiological study of bioactive peptides of food origin; and these results contribute to the use of hardened beans in the formulation of nutraceutical foods to treat hypertension, type 2 diabetes and obesity.