Genotipagem do gene kdr e análise de resistência de larvas e adultos de Aedes aegypti a inseticidas, em Goiânia- GO.

• Autor(a) principal: Chapadense, Francesca Guaracyaba Garcia
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/3400
• topic: Kdr; Aedes aegypti; Inseticidas; Resistência; Resistance; Inseticides; SAUDE COLETIVA::SAUDE PUBLICA

Organophosphorus and Pyrethroids are widely used in Brazil in the control of Aedes aegypti. Excessive use of these insecticides has resulted in the evolution of insecticide-resistant mosquito populations. The present study aimed to evaluate resistance to temephos and deltamethrin of A. aegypti populations from three districts in Goiânia-GO, Brazil (Finsocial, Sudoeste and Jardim America), to and to identify mutations in the kdr resistance-associated sodium channel gene pyrethroid. The collections were made using Ovitraps. The Dose-Response Bioassays were performed according to the WHO. Determined the lethal concentration (LC50 and LC95) for the three populations of the two compounds and their resistance ratios (RR) compared with the susceptible Rockefeller strain. DNA from 30 individuals of each population, and was extracted by using the allele-specific PCR, by dissociation curve to search Phe1534Cys polymorphism site, and Real-Time PCR and polyacrylamide gel for detection of gene polymorphism Val1016Ile kdr. In addition to the substitutions at sites identified individually, they were detected occurring concurrently. The Finsocial, Sudoeste and Jardim América, populations showed, RR95 of 5.1; 4.9 and 5.3 for temephos RR95 and 112.6, 64.7 and 75.9 for deltamethrin, respectively. The Phe1534Cys polymorphism was found in all populations having an allelic frequency: 1.0, 1.0 and 0.98 for Vila Finsocial, Sudoeste and Jardim América. The Val1016Ile polymorphism was detected and showed allele frequency of 0.47, 0.72 and 0.53 respectively in Vila Finsocial, Sudoeste and Jardim América. All populations showed concurrent evaluated polymorphisms occurring in the ratio of 0.25, 0.577 and 0.393 for Vila Finsocial, Southwest and Jardim America. We conclude that even after eight years of disuse of temephos in the region, the larvae of A. aegypti also show resistance to the compound, preventing his return. The deltamethrin is a measure of ineffective management for vector control in Goiânia-GO and mutations involved in the mechanism of resistance to pyrethroids are present at high level in all tested populations, jeopardizing the effectiveness of the main tool for vector control dengue virus.