Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína

• Autor(a) principal: Silva, Dayane Moreira da
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/00130000036pm
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9836
• topic: Memora nodosa; Alantoína; Antiulcerogênica; Allantoin; Antiulcerogenic; FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS

Memora nodosa (Silva Manso) Miers (Bignoniaceae) is a Cerrado plant popularly known as caroba. In traditional medicine leaves and roots are used by the wound healing properties and the roots for abdominal pain. From ethanolic root extract of Memora nodosa (EERMN) was extracted a purinic derivative, allantoin, that is founded in products for topical use by the wound healing properties. Previous works revealed anti-inflammatory and antinociceptive properties with EERMN and allantoin. In this context, we seek evidence of whether, in addition to antiinflammatory action, there would be some gastric damaging effect. However, after the initial results obtained, this work sought to evaluate the antiulcerogenic effect of the extract and the allantoin and elicit the mechanisms involved in this activity. Albino Swiss mice, weighing 30-35g, maintained on standard conditions of temperature (23  1C) and illumination (12-h light/12-h dark cycle) were used. The gastroprotective activity was designed in different experimental gastric ulcers models induced by food restriction, indomethacin, stress, ethanol, ethanol acidity and acetic acid. Seven days after food restriction the animals treated with EERMN (300 mg/kg p.o.) showed reduction in the index of lesion from 15.01  2.92 to 5.20  1.44. In indomethacin induced gastric lesion, different EERMN doses (100, 300 and 1000 mg/kg p.o.) reduced the index of lesion from 12.70  1.70 (control) to 7.20  0.35; 6.37  0.41 and 5.71  0.47 respectively. Seven days after food restriction the animals treated with EERMN (300 mg/kg p. o.) showed reduction in the lesion index from 15.01  2.92 to 5.20  1.44. When the gastric ulcers were induced by ethanol 75%, the EERMN reduced the ulcerated area in 69%. The gastric acid secretion parameters (volume, pH and total acidity) did not alter by the treatment with the plant extract. However, the treatment with EERMN was capable to avoid the gastric mucus barrier depletion when compared to control lesion group, although it was not able to alter the glutathione levels. In the ethanol acidity model, the treatment with allantoin in doses of 30, 60 and 120 mg/kg reduced ulcerated area from 67.08  3.07% to 51.53  4.67; 42.32  4.93 and 43.18  4.90% respectively. When the lesions were induced by ethanol, allantoin (60 mg/kg p.o.) reduced ulcerated area in 80%. In models of acute lesions induced by stress and indomethacin, this same allantoin dose reduced the index of lesion from 11.90  0.93 to 6.62  0.49 and from 15.35  0.81 to 7.09  0.96 respectively. In the chronic model, allantoin demonstrated an antiulcerogenic effect by the reduction of lesion area from 74.41  4.57 to 24.10  0.89 mm2 in the acetic acid model. In the quantification of gastric mucus, similarly to EERMN, allantoin was able to prevent the mucus depletion (34.77  4.14) when compared with control lesion group (19.75  2.53). Moreover, allantoin was able to restore the activity of catalase enzyme from 403.7  15.1 (control lesion group) to 499.9  16.38 nmol/mg protein after gastric lesion induced by ethanol. These results demonstrate antiulcerogenic activity for EERMN and allantoin throughout different mechanisms.