Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade

• Autor(a) principal: Penha, Jaqueline Rodrigues da
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/11905
• topic: Alisantes capilares; Irritação ocular; Teste de Draize; Hair straightener; Eye Irritation; Draize test; CIENCIAS DA SAUDE::FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS

Introduction: The evaluation of ocular toxicity is a mandatory parameter for the classification and labeling of products that may come into contact with the ocular surface, and is carried out according to the extent and depth of the damage caused after exposure. Currently, no model is capable of predicting mild and reversible damage to the cornea that would allow continuous and repeated ex-posures. Objective:! Standardize the cultivation of bovine cornea, in an air-liquid interface, evaluat-ing parameters of tissue viability and cell proliferation, after exposure to chemical and pharmaceuti-cal products. Methodology: Bovine eyes were collected from recently slaughtered animals in a slaughterhouse (Goiânia-GO). Central parts of the corneas were removed and cultivated in DMEM culture medium, supplemented with 5% bovine fetal serum and 10ng/mL of epidermal growth factor at 37°C and atmosphere 5% CO2. Initially, the model was exposed to ten chemical substances of different categories and evaluated by tissue viability assay (MTT). The model was also exposed to samples of capillary straighteners of commercial use nebulized by the Vitrocell® Cloud 12 exposure chamber and evaluated for tissue viability via MTT. Hitological analysis and expression of intracellu-lar formation of Reactive Oxygen Species (ROs) were performed, via DCFH- DA. Results: The model was viable for six days in air-liquid interface and was able to distinguish categories1/2. Expo-sure to Category 2A/2B substances showed a more significant reduction in tissue viability in the 48 hours following exposure, and not immediately after exposure. All samples of commercial use of capillary straightener were cytotoxic. Furthermore, the analysis of one of the samples demonstrated histopathological changes and an increase in intracellular production of ROs. Conclusion: The model was viable during six days of cultivation, being able to distinguish substances from different categories of ocular irritation. In addition, the model enables support for studies with continuous and repeated exposures, being a promising model for occupational toxicity data.