Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/11905 |
Resumo: | Introduction: The evaluation of ocular toxicity is a mandatory parameter for the classification and labeling of products that may come into contact with the ocular surface, and is carried out according to the extent and depth of the damage caused after exposure. Currently, no model is capable of predicting mild and reversible damage to the cornea that would allow continuous and repeated ex-posures. Objective:! Standardize the cultivation of bovine cornea, in an air-liquid interface, evaluat-ing parameters of tissue viability and cell proliferation, after exposure to chemical and pharmaceuti-cal products. Methodology: Bovine eyes were collected from recently slaughtered animals in a slaughterhouse (Goiânia-GO). Central parts of the corneas were removed and cultivated in DMEM culture medium, supplemented with 5% bovine fetal serum and 10ng/mL of epidermal growth factor at 37°C and atmosphere 5% CO2. Initially, the model was exposed to ten chemical substances of different categories and evaluated by tissue viability assay (MTT). The model was also exposed to samples of capillary straighteners of commercial use nebulized by the Vitrocell® Cloud 12 exposure chamber and evaluated for tissue viability via MTT. Hitological analysis and expression of intracellu-lar formation of Reactive Oxygen Species (ROs) were performed, via DCFH- DA. Results: The model was viable for six days in air-liquid interface and was able to distinguish categories1/2. Expo-sure to Category 2A/2B substances showed a more significant reduction in tissue viability in the 48 hours following exposure, and not immediately after exposure. All samples of commercial use of capillary straightener were cytotoxic. Furthermore, the analysis of one of the samples demonstrated histopathological changes and an increase in intracellular production of ROs. Conclusion: The model was viable during six days of cultivation, being able to distinguish substances from different categories of ocular irritation. In addition, the model enables support for studies with continuous and repeated exposures, being a promising model for occupational toxicity data. |
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Valadares, Marize Camposhttp://lattes.cnpq.br/6157755243167018Valadares, Marize CamposCunha, Luiz Carlos daAndrade, Wanessa Machadohttp://lattes.cnpq.br/7953484567057189Penha, Jaqueline Rodrigues da2022-02-24T13:08:28Z2022-02-24T13:08:28Z2021-07-13PENHA , J. R. Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade. 2021. 100 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2021.http://repositorio.bc.ufg.br/tede/handle/tede/11905Introduction: The evaluation of ocular toxicity is a mandatory parameter for the classification and labeling of products that may come into contact with the ocular surface, and is carried out according to the extent and depth of the damage caused after exposure. Currently, no model is capable of predicting mild and reversible damage to the cornea that would allow continuous and repeated ex-posures. Objective:! Standardize the cultivation of bovine cornea, in an air-liquid interface, evaluat-ing parameters of tissue viability and cell proliferation, after exposure to chemical and pharmaceuti-cal products. Methodology: Bovine eyes were collected from recently slaughtered animals in a slaughterhouse (Goiânia-GO). Central parts of the corneas were removed and cultivated in DMEM culture medium, supplemented with 5% bovine fetal serum and 10ng/mL of epidermal growth factor at 37°C and atmosphere 5% CO2. Initially, the model was exposed to ten chemical substances of different categories and evaluated by tissue viability assay (MTT). The model was also exposed to samples of capillary straighteners of commercial use nebulized by the Vitrocell® Cloud 12 exposure chamber and evaluated for tissue viability via MTT. Hitological analysis and expression of intracellu-lar formation of Reactive Oxygen Species (ROs) were performed, via DCFH- DA. Results: The model was viable for six days in air-liquid interface and was able to distinguish categories1/2. Expo-sure to Category 2A/2B substances showed a more significant reduction in tissue viability in the 48 hours following exposure, and not immediately after exposure. All samples of commercial use of capillary straightener were cytotoxic. Furthermore, the analysis of one of the samples demonstrated histopathological changes and an increase in intracellular production of ROs. Conclusion: The model was viable during six days of cultivation, being able to distinguish substances from different categories of ocular irritation. In addition, the model enables support for studies with continuous and repeated exposures, being a promising model for occupational toxicity data.Introdução: A avaliação da toxicidade ocular é um parâmetro obrigatório para classificação e rotu-lagem de produtos que podem entrar em contato com a superfície ocular e é feita de acordo com a extensão e profundidade dos danos causados após exposição. Atualmente, nenhum modelo é ca-paz de prever danos leves e reversíveis à córnea e que possibilitem exposições contínuas e repeti-das. Objetivo: Padronizar o cultivo de córnea bovina, em interface ar-líquido, avaliando parâmetros de viabilidade tecidual e proliferação celular, após exposições a substâncias de diferentes classes e produtos acabados. Métodologia: Olhos bovinos foram cedidos pelo Frigorífico JBS (Goiânia-GO). Partes centrais das córneas foram removidas e cultivadas em meio de cultura DMEM, suplementa-do com 5% de Soro Fetal Bovino e 10ng/mL de fator de crescimento epidermal à 37°C e atmosfera 5% CO2. Inicialmente, o modelo foi exposto à dez substâncias químicas de diferentes categorias e avaliado por meio do ensaio de viabilidade tecidual (MTT). O modelo também foi exposto a amos-tras de alisantes capilares de uso comercial nebulizadas pela câmara de exposição Vitrocell® Cloud 12 e avaliado quanto à viabilidade tecidual via MTT. Foi realizada a análise hitológica e expressão de formação intracelular de Espécies Reativas de Oxigênio (EROs), via DCFH-DA. Resultados: O modelo apresentou-se viável durante seis dias em interface ar-líquido e conseguiu distinguir cate-gorias1/2. A exposição à substâncias de Categoria 2A/2B, demonstrou redução na viabilidade teci-dual de forma mais expressiva nas 48 horas subsequentes à exposição, e não logo após a expo-sição. Todas as amostras de uso comercial de alisante capilar apresentaram- se citotóxicas. Além disso, a análise de uma das amostras demonstrou alterações histopatológicas e aumento na pro-dução intracelular de EROs. Conclusões: O modelo apresentou-se viável durante seis dias de cultivo, sendo capaz de distinguir substâncias de diferentes categorias de irritantação ocular. Além disso, o modelo possibilita embasamento para estudos com exposições continuas e repetidas, sen-do promissor modelo para dados de toxicidade ocupacional.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2022-02-24T00:50:05Z No. of bitstreams: 2 Dissertação - Jaqueline Rodrigues da Penha - 2021.pdf: 8036602 bytes, checksum: 1878d4c4476b02f2a31c3077ae1c8d0c (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2022-02-24T13:08:28Z (GMT) No. of bitstreams: 2 Dissertação - Jaqueline Rodrigues da Penha - 2021.pdf: 8036602 bytes, checksum: 1878d4c4476b02f2a31c3077ae1c8d0c (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2022-02-24T13:08:28Z (GMT). No. of bitstreams: 2 Dissertação - Jaqueline Rodrigues da Penha - 2021.pdf: 8036602 bytes, checksum: 1878d4c4476b02f2a31c3077ae1c8d0c (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2021-07-13Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Amparo à Pesquisa do Estado de GoiásporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências Farmacêuticas (FF)UFGBrasilFaculdade de Farmácia - FF (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAlisantes capilaresIrritação ocularTeste de DraizeHair straightenerEye IrritationDraize testCIENCIAS DA SAUDE::FARMACIA::ANALISE E CONTROLE E MEDICAMENTOSDesenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidadeDevelopment of an ex vivo model of bovine cornea in an air-liquid interface for ocular toxicity: standardization and applicabilityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis2750050050050050050022512013reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/faa84a5c-0ec5-42e7-a302-07db1d3ae1f8/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/21287534-f813-4f09-9f97-d63bf756585e/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Jaqueline Rodrigues da Penha - 2021.pdfDissertação - Jaqueline Rodrigues da Penha - 2021.pdfapplication/pdf8036602http://repositorio.bc.ufg.br/tede/bitstreams/a43c9911-30f4-4d99-beef-f1ee97d1c99f/download1878d4c4476b02f2a31c3077ae1c8d0cMD53tede/119052022-02-24 10:08:28.364http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/11905http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2022-02-24T13:08:28Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)falseTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |
dc.title.pt_BR.fl_str_mv |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
dc.title.alternative.eng.fl_str_mv |
Development of an ex vivo model of bovine cornea in an air-liquid interface for ocular toxicity: standardization and applicability |
title |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
spellingShingle |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade Penha, Jaqueline Rodrigues da Alisantes capilares Irritação ocular Teste de Draize Hair straightener Eye Irritation Draize test CIENCIAS DA SAUDE::FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
title_short |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
title_full |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
title_fullStr |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
title_full_unstemmed |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
title_sort |
Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade |
author |
Penha, Jaqueline Rodrigues da |
author_facet |
Penha, Jaqueline Rodrigues da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Valadares, Marize Campos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6157755243167018 |
dc.contributor.referee1.fl_str_mv |
Valadares, Marize Campos |
dc.contributor.referee2.fl_str_mv |
Cunha, Luiz Carlos da |
dc.contributor.referee3.fl_str_mv |
Andrade, Wanessa Machado |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7953484567057189 |
dc.contributor.author.fl_str_mv |
Penha, Jaqueline Rodrigues da |
contributor_str_mv |
Valadares, Marize Campos Valadares, Marize Campos Cunha, Luiz Carlos da Andrade, Wanessa Machado |
dc.subject.por.fl_str_mv |
Alisantes capilares Irritação ocular Teste de Draize |
topic |
Alisantes capilares Irritação ocular Teste de Draize Hair straightener Eye Irritation Draize test CIENCIAS DA SAUDE::FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
dc.subject.eng.fl_str_mv |
Hair straightener Eye Irritation Draize test |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
description |
Introduction: The evaluation of ocular toxicity is a mandatory parameter for the classification and labeling of products that may come into contact with the ocular surface, and is carried out according to the extent and depth of the damage caused after exposure. Currently, no model is capable of predicting mild and reversible damage to the cornea that would allow continuous and repeated ex-posures. Objective:! Standardize the cultivation of bovine cornea, in an air-liquid interface, evaluat-ing parameters of tissue viability and cell proliferation, after exposure to chemical and pharmaceuti-cal products. Methodology: Bovine eyes were collected from recently slaughtered animals in a slaughterhouse (Goiânia-GO). Central parts of the corneas were removed and cultivated in DMEM culture medium, supplemented with 5% bovine fetal serum and 10ng/mL of epidermal growth factor at 37°C and atmosphere 5% CO2. Initially, the model was exposed to ten chemical substances of different categories and evaluated by tissue viability assay (MTT). The model was also exposed to samples of capillary straighteners of commercial use nebulized by the Vitrocell® Cloud 12 exposure chamber and evaluated for tissue viability via MTT. Hitological analysis and expression of intracellu-lar formation of Reactive Oxygen Species (ROs) were performed, via DCFH- DA. Results: The model was viable for six days in air-liquid interface and was able to distinguish categories1/2. Expo-sure to Category 2A/2B substances showed a more significant reduction in tissue viability in the 48 hours following exposure, and not immediately after exposure. All samples of commercial use of capillary straightener were cytotoxic. Furthermore, the analysis of one of the samples demonstrated histopathological changes and an increase in intracellular production of ROs. Conclusion: The model was viable during six days of cultivation, being able to distinguish substances from different categories of ocular irritation. In addition, the model enables support for studies with continuous and repeated exposures, being a promising model for occupational toxicity data. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-07-13 |
dc.date.accessioned.fl_str_mv |
2022-02-24T13:08:28Z |
dc.date.available.fl_str_mv |
2022-02-24T13:08:28Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PENHA , J. R. Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade. 2021. 100 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2021. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/11905 |
identifier_str_mv |
PENHA , J. R. Desenvolvimento de modelo ex vivo (Nov-ex) de córnea bovina em inferface ar-líquido para avaliação de toxicidade ocular: padronização e aplicabilidade. 2021. 100 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2021. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/11905 |
dc.language.iso.fl_str_mv |
por |
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por |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Goiás |
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Programa de Pós-graduação em Ciências Farmacêuticas (FF) |
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UFG |
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Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Farmácia - FF (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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