Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/11070 |
Resumo: | The present study aimed to evaluate the in vitro and in vivo efficacy of different disinfectants against Trypanosoma vivax. In the in vitro phase, 21 disinfectant solutions were tested in quintuplicate. Disinfectants added to micro tubes containing blood with approximately 1x106(1.000.000) trypomastigotes of T. vivax were evaluated at the 30 seconds, one minute, 10, 15 and 30 minutes of homogenization. After the feasibility evaluation from the thick drop technique, the mobile trypomastigotes were quantified for the calculation of efficacy. In the in vivo step, only disinfectants that demonstrated 100% efficacy and easy acquisition in vitro against the protozoan in question were used. To that end, 30 calves, crossbreed, three to six months old, negative for T. vivax (thick drop, Woo and cPCR) were divided into six different groups of five animals each, which were then inoculated with 46% and 70% alcohol disinfectant solutions or iodine 0.5% + 1x106 trypomastigotes of the protozoan in question at the time of 30 seconds or 1 minute of preparation. One group of animals was inoculated with only 1x106 T. vivax trypomastigotes (positive control), while another group of cattle were not infected (negative control). In order to diagnose the possible presence of T. vivax in the blood stream of the calves, on the 7th, 14th and 21st day after inoculation (DPI), blood was collected from each animal to investigate the protozoan by the techniques of Woo, Brener and cPCR. Based on the results, it can be concluded that in the in vitro stage, 13 of the 21 disinfectant solutions tested demonstrated 100% efficacy against T. vivax at all times of evaluation. Among these 13, alcohol 46%, alcohol 70% and iodine 0.5% were selected for in vivo stage. When the efficiency of these three disinfectants was evaluated in vivo, it was possible to verify that 60% and 20%, respectively, became infected by the protozoan in question, when inoculated with the total solution containing alcohol 46% + 1x106 trypomastigotes of T. vivax, after of 30 seconds and 1 minute of disinfection process. These results highlight the importance of performing the in vivo step in cattle when evaluating the immediate efficacy of disinfectants against T. vivax. |
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Lopes, Welber Daniel Zanettihttp://lattes.cnpq.br/4480577038117234Borges, Ligia Mirandahttp://lattes.cnpq.br/0186857706444299Lopes, Welber Daniel ZanettiFelippelli, GustavoTeixeira, Weslen Fabricio Pireshttp://lattes.cnpq.br/7185946583433370Couto, Luiz Fellipe Monteiro2021-01-27T14:56:54Z2021-01-27T14:56:54Z2019-03-01COUTO, L. F. M. Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax. 2019. 45 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2019.http://repositorio.bc.ufg.br/tede/handle/tede/11070The present study aimed to evaluate the in vitro and in vivo efficacy of different disinfectants against Trypanosoma vivax. In the in vitro phase, 21 disinfectant solutions were tested in quintuplicate. Disinfectants added to micro tubes containing blood with approximately 1x106(1.000.000) trypomastigotes of T. vivax were evaluated at the 30 seconds, one minute, 10, 15 and 30 minutes of homogenization. After the feasibility evaluation from the thick drop technique, the mobile trypomastigotes were quantified for the calculation of efficacy. In the in vivo step, only disinfectants that demonstrated 100% efficacy and easy acquisition in vitro against the protozoan in question were used. To that end, 30 calves, crossbreed, three to six months old, negative for T. vivax (thick drop, Woo and cPCR) were divided into six different groups of five animals each, which were then inoculated with 46% and 70% alcohol disinfectant solutions or iodine 0.5% + 1x106 trypomastigotes of the protozoan in question at the time of 30 seconds or 1 minute of preparation. One group of animals was inoculated with only 1x106 T. vivax trypomastigotes (positive control), while another group of cattle were not infected (negative control). In order to diagnose the possible presence of T. vivax in the blood stream of the calves, on the 7th, 14th and 21st day after inoculation (DPI), blood was collected from each animal to investigate the protozoan by the techniques of Woo, Brener and cPCR. Based on the results, it can be concluded that in the in vitro stage, 13 of the 21 disinfectant solutions tested demonstrated 100% efficacy against T. vivax at all times of evaluation. Among these 13, alcohol 46%, alcohol 70% and iodine 0.5% were selected for in vivo stage. When the efficiency of these three disinfectants was evaluated in vivo, it was possible to verify that 60% and 20%, respectively, became infected by the protozoan in question, when inoculated with the total solution containing alcohol 46% + 1x106 trypomastigotes of T. vivax, after of 30 seconds and 1 minute of disinfection process. These results highlight the importance of performing the in vivo step in cattle when evaluating the immediate efficacy of disinfectants against T. vivax.O presente estudo teve como objetivo, avaliar in vitro e in vivo a eficácia de diferentes desinfetantes contra Trypanosoma vivax. Na etapa laboratorial, in vitro, 21 soluções desinfetantes foram testadas em quintuplicada. Os desinfetantes adicionados a micro tubos contendo sangue com aproximadamente 1x106(1.000.000) tripomastigotas de T. vivax, foram avaliados nos intervalos de 30 segundos, um minuto, 10, 15 e 30 minutos de homogeneização. Após a avaliação da viabilidade a partir da técnica da gota espessa, as tripomastigostas móveis foram quantificadas para o cálculo de eficácia. Na etapa in vivo, foram utilizados apenas os desinfetantes que demonstraram 100% de eficácia e fácil aquisição contra o protozoário em questão na etapa in vitro. Para tal, 30 bezerros, mestiços, de três a seis meses de idade, negativos para T. vivax (gota espessa, Woo e cPCR,) foram divididos em seis diferentes grupos, de cinco animais cada, que foram posteriormente inoculados com soluções de desinfetantes de álcool 46% e 70% ou iodo 0,5% + 1x106 tripomastigotas do protozoário em questão, nos tempos de 30 segundos ou 1 minuto de preparo. Um grupo de animais foi inoculado apenas com 1x106 tripomastigotas de T. vivax (controle postivo), enquanto outro grupo de bovinos não foi infectado (controle negativo). Com o objetivo de se diagnosticar a possível presença de T. vivax na corrente sanguínea dos bezerros, nos 7º, 14º e 21º dia pós-inoculação (DPI), foi colhido sangue de cada animal para pesquisa do referido protozoário pelas técnicas de Woo, Brener e cPCR. Com base nos resultados encontrados, pode-se concluir que na etapa in vitro, 13 das 21 soluções desinfetantes testadas demonstraram 100% de eficácia contra T. vivax, em todos os tempos de avaliação. Dentre estes 13, o álcool 46%, álcool 70% e iodo 0,5% foram selecionados para etapa in vivo. Quando a eficiência destes três desinfetantes foi avaliada in vivo, foi possível inferir que 60% e 40% dos bovinos, respectivamente, se infectaram pelo protozoário em questão, o ao serem inoculados com a solução total contendo álcool 46% + 1x106 tripomastigotas de T. vivax, depois de 30 segundos e 1 minuto de processo de desinfecção. Estes resultados destacam a importância de realizar a etapa in vivo em bovinos, quando se pretende avaliar a eficácia imediata de desinfetantes contra T. vivax.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2021-01-27T13:18:08Z No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Dissertação - Luiz Fellipe Monteiro Couto - 2019.pdf: 2025462 bytes, checksum: 9f3fbf6f7188efb97a30f4506eb37c51 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2021-01-27T14:56:54Z (GMT) No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Dissertação - Luiz Fellipe Monteiro Couto - 2019.pdf: 2025462 bytes, checksum: 9f3fbf6f7188efb97a30f4506eb37c51 (MD5)Made available in DSpace on 2021-01-27T14:56:54Z (GMT). No. of bitstreams: 2 license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Dissertação - Luiz Fellipe Monteiro Couto - 2019.pdf: 2025462 bytes, checksum: 9f3fbf6f7188efb97a30f4506eb37c51 (MD5) Previous issue date: 2019-03-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessDesinfecçãoHemoparasitoTripanossomoseHigienizaçãoDisinfectionHemoparasiteSanitationTrypanosomiasisCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE PRODUTOS DE ORIGEM ANIMALAvaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivaxIn vitro and in vivo evaluation of disinfectants against Trypanosoma vivaxinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis18500500500500611991reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/566746a5-52d8-47e4-abed-f8740fe1aa9c/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/88f422b8-948d-41fc-8ed5-b18f9c53b2f3/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDissertação - Luiz Fellipe Monteiro Couto - 2019.pdfDissertação - Luiz Fellipe Monteiro Couto - 2019.pdfapplication/pdf2025462http://repositorio.bc.ufg.br/tede/bitstreams/8d98de0d-a874-4a1b-be69-8b14f4bfda40/download9f3fbf6f7188efb97a30f4506eb37c51MD53tede/110702021-01-27 11:56:54.968http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/11070http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2021-01-27T14:56:54Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
dc.title.alternative.eng.fl_str_mv |
In vitro and in vivo evaluation of disinfectants against Trypanosoma vivax |
title |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
spellingShingle |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax Couto, Luiz Fellipe Monteiro Desinfecção Hemoparasito Tripanossomose Higienização Disinfection Hemoparasite Sanitation Trypanosomiasis CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE PRODUTOS DE ORIGEM ANIMAL |
title_short |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
title_full |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
title_fullStr |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
title_full_unstemmed |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
title_sort |
Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax |
author |
Couto, Luiz Fellipe Monteiro |
author_facet |
Couto, Luiz Fellipe Monteiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lopes, Welber Daniel Zanetti |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4480577038117234 |
dc.contributor.advisor-co1.fl_str_mv |
Borges, Ligia Miranda |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0186857706444299 |
dc.contributor.referee1.fl_str_mv |
Lopes, Welber Daniel Zanetti |
dc.contributor.referee2.fl_str_mv |
Felippelli, Gustavo |
dc.contributor.referee3.fl_str_mv |
Teixeira, Weslen Fabricio Pires |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7185946583433370 |
dc.contributor.author.fl_str_mv |
Couto, Luiz Fellipe Monteiro |
contributor_str_mv |
Lopes, Welber Daniel Zanetti Borges, Ligia Miranda Lopes, Welber Daniel Zanetti Felippelli, Gustavo Teixeira, Weslen Fabricio Pires |
dc.subject.por.fl_str_mv |
Desinfecção Hemoparasito Tripanossomose Higienização |
topic |
Desinfecção Hemoparasito Tripanossomose Higienização Disinfection Hemoparasite Sanitation Trypanosomiasis CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE PRODUTOS DE ORIGEM ANIMAL |
dc.subject.eng.fl_str_mv |
Disinfection Hemoparasite Sanitation Trypanosomiasis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE PRODUTOS DE ORIGEM ANIMAL |
description |
The present study aimed to evaluate the in vitro and in vivo efficacy of different disinfectants against Trypanosoma vivax. In the in vitro phase, 21 disinfectant solutions were tested in quintuplicate. Disinfectants added to micro tubes containing blood with approximately 1x106(1.000.000) trypomastigotes of T. vivax were evaluated at the 30 seconds, one minute, 10, 15 and 30 minutes of homogenization. After the feasibility evaluation from the thick drop technique, the mobile trypomastigotes were quantified for the calculation of efficacy. In the in vivo step, only disinfectants that demonstrated 100% efficacy and easy acquisition in vitro against the protozoan in question were used. To that end, 30 calves, crossbreed, three to six months old, negative for T. vivax (thick drop, Woo and cPCR) were divided into six different groups of five animals each, which were then inoculated with 46% and 70% alcohol disinfectant solutions or iodine 0.5% + 1x106 trypomastigotes of the protozoan in question at the time of 30 seconds or 1 minute of preparation. One group of animals was inoculated with only 1x106 T. vivax trypomastigotes (positive control), while another group of cattle were not infected (negative control). In order to diagnose the possible presence of T. vivax in the blood stream of the calves, on the 7th, 14th and 21st day after inoculation (DPI), blood was collected from each animal to investigate the protozoan by the techniques of Woo, Brener and cPCR. Based on the results, it can be concluded that in the in vitro stage, 13 of the 21 disinfectant solutions tested demonstrated 100% efficacy against T. vivax at all times of evaluation. Among these 13, alcohol 46%, alcohol 70% and iodine 0.5% were selected for in vivo stage. When the efficiency of these three disinfectants was evaluated in vivo, it was possible to verify that 60% and 20%, respectively, became infected by the protozoan in question, when inoculated with the total solution containing alcohol 46% + 1x106 trypomastigotes of T. vivax, after of 30 seconds and 1 minute of disinfection process. These results highlight the importance of performing the in vivo step in cattle when evaluating the immediate efficacy of disinfectants against T. vivax. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-03-01 |
dc.date.accessioned.fl_str_mv |
2021-01-27T14:56:54Z |
dc.date.available.fl_str_mv |
2021-01-27T14:56:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COUTO, L. F. M. Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax. 2019. 45 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2019. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/11070 |
identifier_str_mv |
COUTO, L. F. M. Avaliação in vitro e in vivo da eficácia de desinfetantes contra Trypanosoma vivax. 2019. 45 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2019. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/11070 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
18 |
dc.relation.confidence.fl_str_mv |
500 500 500 500 |
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6 |
dc.relation.cnpq.fl_str_mv |
1199 |
dc.relation.sponsorship.fl_str_mv |
1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciência Animal (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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Universidade Federal de Goiás (UFG) |
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UFG |
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