Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/5049 |
Resumo: | Staphylococcus saprophyticus is characterized as uropathogenic bacteria that causes urinary tract infections especially in young women. Some virulence factors were elucidated, however, little is known about how this bacteria install itself at host human. The urease was the first virulence factor described in S. saprophyticus, being responsible by increasing of the bladder pH in infected patients. This is the first study about S. saprophyticus in proteomics and immunoproteomics perspective of the secreted proteins (exoproteome) of this bacterium. A total of 44 new secreted proteins were detected by mass spectrometry. Among the proteins found, five of them have a crucial role in the glycolytic pathway: Triosephosphate isomerase (TPI), enolase (ENO), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glucose-6-phosphate isomerase (GPI), however, its role as moonlighting proteins have been observed in various microorganisms. Through of the immunoproteomics, it was possible to detect 18 protein species that reacted onto Western-blotting, and 5 of them could be identified by mass spectrometry. The most abundant protein specie identified as immunogenic in S. saprophyticus, it was transglycosilase IsaA (Immunodominant staphylococcal antigen A), being it well described in Staphylococcus aureus as virulence factor. Another abundant protein found as immunogenic was Ssa (Staphylococcal secretory antigen), which, it was identified under 3 isoforms. The enolase was the last protein identified at immunoproteome of S. saprophyticus. It was possible to conclude from these results that S. saprophyticus has a wide of extracellular proteins capable to promote bacteria adaption, and some of them are involved in oxidative/nitrosative stress (SOD and AhpC), besides possess proteins that have the capacity to incite the humoral immune response in BalbC mice. All this lead us to hypothesize that S. saprophyticus have defense and virulence mechanisms in order to protect itself against extracellular stresses and appropriate mechanisms to promote urinary tract infections. |
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Bailão , Alexandre Melohttp://lattes.cnpq.br/5415221996976886Rocha, Juliana Alves Parentehttp://lattes.cnpq.br/7089231795367245Bailão, Alexandre MeloMarval, Márcia Giambiagi deSoares, Célia Maria de AlmeidaRocha, Juliana Alves Parentehttp://lattes.cnpq.br/3023497366488504Oliveira, Lucas Silva de2015-12-11T07:57:23Z2014-04-23OLIVEIRA, L. S. Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus. 2014. 81 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/5049Staphylococcus saprophyticus is characterized as uropathogenic bacteria that causes urinary tract infections especially in young women. Some virulence factors were elucidated, however, little is known about how this bacteria install itself at host human. The urease was the first virulence factor described in S. saprophyticus, being responsible by increasing of the bladder pH in infected patients. This is the first study about S. saprophyticus in proteomics and immunoproteomics perspective of the secreted proteins (exoproteome) of this bacterium. A total of 44 new secreted proteins were detected by mass spectrometry. Among the proteins found, five of them have a crucial role in the glycolytic pathway: Triosephosphate isomerase (TPI), enolase (ENO), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glucose-6-phosphate isomerase (GPI), however, its role as moonlighting proteins have been observed in various microorganisms. Through of the immunoproteomics, it was possible to detect 18 protein species that reacted onto Western-blotting, and 5 of them could be identified by mass spectrometry. The most abundant protein specie identified as immunogenic in S. saprophyticus, it was transglycosilase IsaA (Immunodominant staphylococcal antigen A), being it well described in Staphylococcus aureus as virulence factor. Another abundant protein found as immunogenic was Ssa (Staphylococcal secretory antigen), which, it was identified under 3 isoforms. The enolase was the last protein identified at immunoproteome of S. saprophyticus. It was possible to conclude from these results that S. saprophyticus has a wide of extracellular proteins capable to promote bacteria adaption, and some of them are involved in oxidative/nitrosative stress (SOD and AhpC), besides possess proteins that have the capacity to incite the humoral immune response in BalbC mice. All this lead us to hypothesize that S. saprophyticus have defense and virulence mechanisms in order to protect itself against extracellular stresses and appropriate mechanisms to promote urinary tract infections.Staphylococcus saprophyticus caracteriza-se por ser uma bactéria uropatogênica que causa infecção no trato genito-urinário especialmente de mulheres jovens. Alguns fatores de virulência já foram elucidados, no entanto, pouco se conhece do modo pelo qual esta bactéria acomete o hospedeiro humano. A uréase foi o primeiro fator de virulência descrito em S. saprophyticus, sendo responsável pela alcalinização do pH da bexiga de pacientes contaminados. Este estudo é o pioneiro tratando-se de S. saprophyticus na abordagem da proteômica e imunoproteômica do perfil de proteínas secretadas (exoproteoma) por esta bactéria. Um total de 44 proteínas secretadas inéditas foram detectadas por espectrometria de massas. Dentre as proteínas encontradas, cinco delas possuem papel fundamental na via glicolítica: triose-fosfato isomerase (TPI), enolase (ENO), gliceraldeído-3-fosfato-desidrogenase (GAPDH) e glicose-6-fosfato isomerase (GPI), no entanto, seu papel como proteína “moonlighting” já foi observado em diversos microrganismos. Através da imunoproteômica, foi possível detectar 18 espécies proteicas que reagiram no Wester-blotting, e 5 delas puderam ser identificadas por espectrometria de massas. A espécie proteica mais abundante identificada como imunogênica em S. saprophyticus, foi a transglicosilase IsaA (Immunodominant staphylococcal antigen A), sendo bem descrita em S. aureus como um fator de virulência. Outra proteína abundante identificada como imunogênica foi a Ssa (Staphylococcal secretory antigen), no qual, foi identificada em 3 isoformas. A enolase também foi identificada no imunoproteoma de S. saprophyticus. Foi possível concluir através destes resultados que S. saprophyticus possui uma gama de proteínas extracelulares capazes de promover a adaptação desta bactéria, algumas delas envolvidas na proteção contra o estresse oxidativo/nitrosativo (SOD e AhpC), além de possuir proteínas que tem a característica de incitar a resposta imune humoral de camundongos BalbC. Isso tudo nos leva a hipotetizar que S. saprophyticus possui mecanismos de defesa e virulência a fim de se proteger contra estresses exteriores e mecanismos para promover a patogênese no trato genito-urinário.Submitted by Cláudia Bueno (claudiamoura18@gmail.com) on 2015-12-10T14:10:12Z No. of bitstreams: 2 Dissertação - Lucas Silva de Oliveira - 2014.pdf: 3199133 bytes, checksum: cb42a7815c2701477249ff4d0cb2a05b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-12-11T07:57:23Z (GMT) No. of bitstreams: 2 Dissertação - Lucas Silva de Oliveira - 2014.pdf: 3199133 bytes, checksum: cb42a7815c2701477249ff4d0cb2a05b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2015-12-11T07:57:23Z (GMT). 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dc.title.por.fl_str_mv |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
title |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
spellingShingle |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus Oliveira, Lucas Silva de Staphylococcus saprophyticus Exoproteoma Imunoproteômica Transglicosilase IsaA Virulência Staphylococcus saprophyticus Exoproteome Immunoproteomics Transglicosilase IsaA Virulence MICROBIOLOGIA::MICROBIOLOGIA APLICADA |
title_short |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
title_full |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
title_fullStr |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
title_full_unstemmed |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
title_sort |
Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus |
author |
Oliveira, Lucas Silva de |
author_facet |
Oliveira, Lucas Silva de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bailão , Alexandre Melo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5415221996976886 |
dc.contributor.advisor-co1.fl_str_mv |
Rocha, Juliana Alves Parente |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7089231795367245 |
dc.contributor.referee1.fl_str_mv |
Bailão, Alexandre Melo |
dc.contributor.referee2.fl_str_mv |
Marval, Márcia Giambiagi de |
dc.contributor.referee3.fl_str_mv |
Soares, Célia Maria de Almeida |
dc.contributor.referee4.fl_str_mv |
Rocha, Juliana Alves Parente |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3023497366488504 |
dc.contributor.author.fl_str_mv |
Oliveira, Lucas Silva de |
contributor_str_mv |
Bailão , Alexandre Melo Rocha, Juliana Alves Parente Bailão, Alexandre Melo Marval, Márcia Giambiagi de Soares, Célia Maria de Almeida Rocha, Juliana Alves Parente |
dc.subject.por.fl_str_mv |
Staphylococcus saprophyticus Exoproteoma Imunoproteômica Transglicosilase IsaA Virulência Staphylococcus saprophyticus Exoproteome Immunoproteomics Transglicosilase IsaA Virulence |
topic |
Staphylococcus saprophyticus Exoproteoma Imunoproteômica Transglicosilase IsaA Virulência Staphylococcus saprophyticus Exoproteome Immunoproteomics Transglicosilase IsaA Virulence MICROBIOLOGIA::MICROBIOLOGIA APLICADA |
dc.subject.cnpq.fl_str_mv |
MICROBIOLOGIA::MICROBIOLOGIA APLICADA |
description |
Staphylococcus saprophyticus is characterized as uropathogenic bacteria that causes urinary tract infections especially in young women. Some virulence factors were elucidated, however, little is known about how this bacteria install itself at host human. The urease was the first virulence factor described in S. saprophyticus, being responsible by increasing of the bladder pH in infected patients. This is the first study about S. saprophyticus in proteomics and immunoproteomics perspective of the secreted proteins (exoproteome) of this bacterium. A total of 44 new secreted proteins were detected by mass spectrometry. Among the proteins found, five of them have a crucial role in the glycolytic pathway: Triosephosphate isomerase (TPI), enolase (ENO), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glucose-6-phosphate isomerase (GPI), however, its role as moonlighting proteins have been observed in various microorganisms. Through of the immunoproteomics, it was possible to detect 18 protein species that reacted onto Western-blotting, and 5 of them could be identified by mass spectrometry. The most abundant protein specie identified as immunogenic in S. saprophyticus, it was transglycosilase IsaA (Immunodominant staphylococcal antigen A), being it well described in Staphylococcus aureus as virulence factor. Another abundant protein found as immunogenic was Ssa (Staphylococcal secretory antigen), which, it was identified under 3 isoforms. The enolase was the last protein identified at immunoproteome of S. saprophyticus. It was possible to conclude from these results that S. saprophyticus has a wide of extracellular proteins capable to promote bacteria adaption, and some of them are involved in oxidative/nitrosative stress (SOD and AhpC), besides possess proteins that have the capacity to incite the humoral immune response in BalbC mice. All this lead us to hypothesize that S. saprophyticus have defense and virulence mechanisms in order to protect itself against extracellular stresses and appropriate mechanisms to promote urinary tract infections. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-04-23 |
dc.date.accessioned.fl_str_mv |
2015-12-11T07:57:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, L. S. Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus. 2014. 81 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/5049 |
identifier_str_mv |
OLIVEIRA, L. S. Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus. 2014. 81 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2014. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/5049 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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6085308344741430434 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
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dc.relation.cnpq.fl_str_mv |
-203884118251241698 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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Universidade Federal de Goiás (UFG) |
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UFG |
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Repositório Institucional da UFG |
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Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/0eac9244-55b7-413c-9292-2473b63595a8/download http://repositorio.bc.ufg.br/tede/bitstreams/d952eddf-4d5d-4461-97c4-0936819826e9/download http://repositorio.bc.ufg.br/tede/bitstreams/7ea50f88-246c-47a4-bee4-cfd5e6eea1ac/download http://repositorio.bc.ufg.br/tede/bitstreams/7b1b23e9-0635-4711-a5cf-5ca67f26ee4c/download http://repositorio.bc.ufg.br/tede/bitstreams/da1ea0ab-e5cc-4805-9928-c535d37a3d41/download http://repositorio.bc.ufg.br/tede/bitstreams/9ec9d667-db43-48b8-b157-3693b206e96e/download http://repositorio.bc.ufg.br/tede/bitstreams/b3d967a8-42ba-46bc-b076-4021225d993e/download |
bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f ae2fe251842ade1134c5d9bb99b6eefe d41d8cd98f00b204e9800998ecf8427e cb42a7815c2701477249ff4d0cb2a05b 98abcd42af83965bb5146701ec0e4e0d 3d0400eac98bbda23ba58014f063fca2 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044358576963584 |