Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos

Detalhes bibliográficos
Autor(a) principal: Batista, Mariana Pedrosa
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
dARK ID: ark:/38995/00130000083xz
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/3014
Resumo: Rural workers are constantly exposed to pesticides they use on crops, and this exposure may be responsible for genetic damage causing a health risk. A problem with the use of pesticides is the genotoxicity, which can lead to the onset of disease. Little is known about the relationship between genotoxicity and the variation of genetic polymorphisms of xenobiotic metabolism that may modify individual susceptibility to the possible genotoxic effects of pesticides. Therefore, there is a need to study genes as glutathione-S-transferase mu (GSTM1) and theta glutathione S-transferase (GSTT1) encoding detoxification enzymes of genotoxic compounds. Another important assessment in individuals exposed to pesticides is the presence of chromosomal translocation t(14;18) (q31,q21), which can be investigated at the molecular level. Thus, this study evaluated the polymorphisms of GSTM1 and GSTT1 genes in 120 individuals occupationally exposed to pesticides and 115 controls (without exposure to pesticides), by real-time PCR. The frequencies of GSTM1 and GSTT1 genotypes were found in 49 % and 18 %, respectively in the exposed group and 37 % and 18 %, respectively, for the control group. It has been found that there is a greater possibility of poisoning in workers who have null genotypes. There was no statistically significant correlation between the increased risk of intoxication and alcohol consumption, smoking and use of PPE. In addition, 29 workers exposed to pesticides for more than 15 years and with null genotypes in GSTT1 and / or GSTM1, were evaluated for the presence of the t (14;18)(q31, q21), in 100 nuclei, by fluorescent in situ hybridization (FISH) . The translocation was observed in only one individual, which may have been caused by prolonged exposure to pesticides, increasing age or alcohol consumption. Thus, the study of genetic polymorphisms and translocations as biomarkers of susceptibility is of fundamental importance in understanding the processes involved in genotype distribution mutagenesis and carcinogenesis and could help minimize the risks for susceptible individuals who are exposed to pesticides.
id UFG-2_e97902c9c7d3210d1187ec43172fe51c
oai_identifier_str oai:repositorio.bc.ufg.br:tde/3014
network_acronym_str UFG-2
network_name_str Repositório Institucional da UFG
repository_id_str
spelling Silva, Daniela de Melo ehttp://lattes.cnpq.br/9895211901348365http://lattes.cnpq.br/2464432014109246Batista, Mariana Pedrosa2014-09-04T19:33:18Z2014-09-042014-02-28BATISTA, Mariana Pedrosa. Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos. 2014. 54 f. Dissertação (Mestrado em Genetica e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tde/3014ark:/38995/00130000083xzRural workers are constantly exposed to pesticides they use on crops, and this exposure may be responsible for genetic damage causing a health risk. A problem with the use of pesticides is the genotoxicity, which can lead to the onset of disease. Little is known about the relationship between genotoxicity and the variation of genetic polymorphisms of xenobiotic metabolism that may modify individual susceptibility to the possible genotoxic effects of pesticides. Therefore, there is a need to study genes as glutathione-S-transferase mu (GSTM1) and theta glutathione S-transferase (GSTT1) encoding detoxification enzymes of genotoxic compounds. Another important assessment in individuals exposed to pesticides is the presence of chromosomal translocation t(14;18) (q31,q21), which can be investigated at the molecular level. Thus, this study evaluated the polymorphisms of GSTM1 and GSTT1 genes in 120 individuals occupationally exposed to pesticides and 115 controls (without exposure to pesticides), by real-time PCR. The frequencies of GSTM1 and GSTT1 genotypes were found in 49 % and 18 %, respectively in the exposed group and 37 % and 18 %, respectively, for the control group. It has been found that there is a greater possibility of poisoning in workers who have null genotypes. There was no statistically significant correlation between the increased risk of intoxication and alcohol consumption, smoking and use of PPE. In addition, 29 workers exposed to pesticides for more than 15 years and with null genotypes in GSTT1 and / or GSTM1, were evaluated for the presence of the t (14;18)(q31, q21), in 100 nuclei, by fluorescent in situ hybridization (FISH) . The translocation was observed in only one individual, which may have been caused by prolonged exposure to pesticides, increasing age or alcohol consumption. Thus, the study of genetic polymorphisms and translocations as biomarkers of susceptibility is of fundamental importance in understanding the processes involved in genotype distribution mutagenesis and carcinogenesis and could help minimize the risks for susceptible individuals who are exposed to pesticides.Os trabalhadores rurais estão constantemente expostos aos agrotóxicos que utilizam nas lavouras, e esta exposição pode ser responsável por danos genéticos causando um risco para a saúde. Um dos problemas da utilização de agrotóxicos é a genotoxicidade, que pode levar ao aparecimento de doenças. Pouco se sabe sobre a relação entre a genotoxicidade e a variação de polimorfismos genéticos de metabolização de xenobióticos que podem modificar a suscetibilidade individual aos possíveis efeitos genotóxicos dos agrotóxicos. Por isso, há a necessidade do estudo de genes como a glutationa-S-tranferase mu (GSTM1) e glutationa-S–transferase teta (GSTT1) que codificam enzimas de detoxificação de compostos genotóxicos. Outra avaliação importante em indivíduos expostos a agrotóxicos é a pesquisa da translocação cromossômica t(14;18)(q31;q21), a qual pode ser investigada em nível molecular. Nesse sentido, esse estudo avaliou os polimorfismos dos genes GSTT1 e GSTM1, em 120 indivíduos ocupacionalmente expostos aos agrotóxicos e de 115 controles (sem exposição aos agrotóxicos), pela metodologia de PCR em tempo real. As frequências de genótipos GSTM1 e GSTT1 encontradas foram de 49% e 18%, respectivamente, para o grupo exposto e 37% e 18%, respectivamente, para o grupo controle. Verificou-se que não há maior possibilidade de intoxicação em trabalhadores que apresentam genótipos nulos. Não houve correlação estatisticamente significativa entre o aumento do risco de intoxicação e o consumo de álcool, tabaco e uso de EPI. Além disso, 29 trabalhadores, expostos a agrotóxicos por mais de 15 anos e com genótipos nulos, GSTT1 e/ou GSTM1, foram avaliados quanto à presença da translocação t(14;18)(q31; q21), pela análise de 100 células, por Hibridação Fluorescente in situ (FISH). Com essa análise, a translocação foi observada em apenas um indivíduo, a qual pode ter sido causada pela exposição prolongada a agrotóxicos, aumento da idade ou consumo de álcool. Dessa forma, o estudo de polimorfismos genéticos e translocações, como biomarcadores de suscetibilidade é de importância fundamental na compreensão dos processos de distribuição genotípica envolvidos na mutagênese e carcinogênese e poderia ajudar a minimizar os riscos para indivíduos suscetíveis que são expostos a agrotóxicos.Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-09-04T19:33:18Z No. of bitstreams: 2 Batista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdf: 1556018 bytes, checksum: 89fbad76ace350a8cf9c7db5c92f015d (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-09-04T19:33:18Z (GMT). No. of bitstreams: 2 Batista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdf: 1556018 bytes, checksum: 89fbad76ace350a8cf9c7db5c92f015d (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-02-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/7167/Batista%2c%20Mariana%20Pedrosa%20-%20Avalia%c3%a7%c3%a3o%20citogen%c3%a9tica%20e%20molecular%20de%20indiv%c3%adduos%20ocupacionalmente%20expostos%20aos%20agrot%c3%b3xicos%20-%202014.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Genetica e Biologia MolecularUFGBrasilInstituto de Ciências Biológicas - ICB (RG)ABBAS, A.; DELVINQUIÈRE, K.; LECHEVREL, M.; LEBAILLY, P. et al. GSTM1, GSTT1, GSTP1 and CYP1A1 genetic polymorphisms and susceptibility to esophageal cancer in a French population: different pattern of squamous cell carcinoma and adenocarcinoma. World J Gastroenterol, v. 10, n. 23, p. 3389-93, 2004. ABDEL-RAHMAN SZ.; EL-ZEIN RA, ANWAR WA.; AU WW. A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies. Cancer Lett.; 10: 229- 233,1996. ABHISHEK, S.; KAUR, N.; KAUR, S.; LATA, M. et al. Association of GSTM1 and GSTT1 gene deletions with susceptibility to DNA damage in the pesticide-exposed workers of Punjab. Rejuvenation Research, v. 13, n. 2-3, p. 281-284, 2010. ABRASCO, Associação Brasileira de Saúde Coletiva. DOSSIÊ ABRASCO – Uma alerta sobre os impactos dos Agrotóxicos na Saúde ABRASCO, Rio de Janeiro, abril de 2012, 1a Parte.98p. Disponível em:<http://www.abrasco.org.br/UserFiles/File ABRASCODIVULGA/2012/DossieAGT.pdf>. Acesso em : 13 de jan,2014, 20:50. ALAVANJA M.C.R, HOPPIN J.A. & KAMEL F.Health effects of chronic pesticide exposure: cancer and neurotoxity. Annu. Rev. Public Health 25:157-197,2004. ANGERER, J.; EWERS, U.; WILHELM, M. Human biomonitoring: state of the art. International journal of hygiene and environmental health, v. 210, n. 3-4, p. 201-228, 2007. ANVISA. Agrotóxico e toxicologia. Site ANVISA, 2011. Disponível em: < http://portal.anvisa.gov.br/wps/portal/anvisa/anvisa/home/agrotoxicotoxicologia >. Acesso em: 08/01/14 ARRUDA, V.R.; GRIGNOLI, C.E.; GONÇALVES, Prevalence of homozygosity for the deleted alleles of glutathione S-transferase mu (GSTM1) and theta (GSTT1) among distinct ethnic groups from Brazil: relevance to environmental carcinogenesis? Clinical Genetics,54:210-214,1998. AYRES M, AYRES JR. M, AYRES DL, SANTOS AS. BioEstat: aplicações estatísticas nas áreas das ciências bio-médicas. Belém: Sociedade Civil Mamirauá; 132-214 p.2007. BAUMAN, J.G.J.; WIEGANT, J.; BORST, P.; DUIJN, P.A new method for microscopial localization of specific DNA sequences by in situ hybridization of fluorchrome-labelled RNA. Experimental Cell Research, v.128, p.485- 490, 1980. BELL, D. A., LIU, Y., CORTOPASSI, G. A. Occurrence of bcl-2 oncogene translocation with increased frequency in the peripheral blood of heavy smokers.J. Natl Cancer Inst. n. 87. p. 223– 224,1995. BENEDETTI.D, NUNES.E, SARMENTO.M, PORTO.C, DIAS.J.F, SANTOS.C.E.I,BLAIR, A.; ZAHM S. H.; PEARCE, N. E.; HEINEMAN, E. F.; FRAUMENI, J. F. Clues to cancer etiology from studies of farmers. Scandinavian Journal of Work, Environment & Health. n. 18. Helsinque, p. 209-215,1992. BOCHNER, R. Sistema Nacional de Informações Tóxico-Farmacológicas–SINITOX e as intoxicações humanas por agrotóxicos no Brasil. Cien Saude Colet, v. 12, n. 1, p. 73-89, 2007. BOLOGNESI, C. Genotoxicity of pesticides: a review of human biomonitoring studies. Mutation Research/Reviews in Mutation Research, v. 543, n. 3, p. 251-272, 2003. BRASIL, Ministério da Saúde. Secretaria de Vigilância Sanitária. Manual de Vigilância da Saúde de Populações Expostas a Agrotóxicos. Organização Pan-Americana de Saúde/Organização Mundial de Saúde. Brasília.1997. BUCHARD, A.; SANCHEZ, J. J.; DALHOFF, K.; MORLING, N.et al. Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants: Simultaneously Detecting GSTM1 and GSTT1 Gene Copy Number and the Allelic Status of the GSTP1 Ile105Val Genetic Variant. The Journal of molecular diagnostics: JMD, v. 9, n. 5, p. 612, 2007. BUFALO NE, LEITE JL, GUILHEN AC, MORARI EC, GRANJA F, ASSUMPCAO LV, WARD LS. Smoking and susceptibility to thyroid cancer: an inverse association with CYP1A1 allelic variants. Endocr Relat Cancer.; 13(4):1185-93,2006. BUGANO, D.; CONFORTI-FROES, N.; YAMAGUCHI, N.; BARACAT, E. Genetic polymorphisms, the metabolism of estrogens and breast cancer: a review. European journal of gynaecological oncology, v. 29, n. 4, p. 313, 2008. BULL, S.; FLETCHER, K.; BOOBIS, A.; BATTERSHILL, J. Evidence for genotoxicity of pesticides in pesticide applicators: a review. Mutagenesis, v. 21, n. 2, p. 93-103, 2006. BURIM, R. V.; CANALLE, R.; MARTINELLI, A. L. C.; TAKAHASHI, C. S. Polymorphisms in glutathione S_transferases GSTM1, GSTT1 and GSTP1 and cytochromes P450 CYP2E1 and CYP1A1 and susceptibility to cirrhosis or pancreatitis in alcoholics. Mutagenesis, v. 19, n. 4, p. 291-298, 2004. BUSTILLOS, N. T.; ASCARRUNZ, M. E.; AGUILAR, X.; RADA, A. Polimorfismos genéticos de la GSTM1 y GSTT1 como modificadores de riesgo mutagénico en agricultores bolivianos expuestos a plaguicidas. BIOFARBO, v. 20, n. 1, 2012. BUTLER, M. W.; HACKETT, N. R.; SALIT, J.; STRULOVICI-BAREL, Y. et al. Glutathione Stransferase copy number variation alters lung gene expression. European Respiratory Journal, v. 38, n. 1, p. 15-28, 2011. CALDERÓN-SEGURA, M. E.; GÓMEZ-ARROYO, S.; VILLALOBOS-PIETRINI, R.; MARTÍNEZ-VALENZUELA, C. et al. Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News. Journal of Toxicology, v. 2012, 2012. CARNEIRO, F. F. P., W; RIGOTTO, R M; AUGUSTO, L G S. RIZZOLO, A; MULLER, N M; ALEXANDRE, V P.; FRIEDRICH, K; MELLO, M S C. Dossiê ABRASCO – Um alerta sobre os impactos dos agrotóxicos na saúde. Parte 1 - Agrotóxicos, Segurança Alimentar e Nutricional e Saúde. Rio de Janeiro: ABRASCO, 2012. CARVALHO, C.A.M.C. Aplicação médico-legal da PCR em tempo real na caracterização de SNPs. Tese de Mestrado, Faculdade de Medicina da Universidade do Porto. Porto, 75 pp.2007. CERRONI L., WIESNER T.. Linfomas cutáneos: de lamorfología a latecnología de microprocesador. ActasDermo-Sifiliográficas Volume 100, Supplement 1, November, p.3–17, 2009. CHANG XIN S., L.H., LIN X., YIRONG L. The detection of circulating tumor cells of breast cancer patients by using multimarker (Survivin, hTERT and hMAM) quantitative real-time PCR.Clinical Biochemistry 42, 194-200, (2010). CHAVES, T. V. S. Dissertação: Avaliação do impacto do uso de agrotóxicos nos trabalhadores rurais dos municípios de ribeiro gonçalves, baixa grande do ribeiro e uruçuí – piauí. Fortaleza, 2007 CHIU, B. C.; DAVE, B. J.; BLAIR, A.; GAPSTUR, S. M.; ZAHM, S. H.; WEISENBURGER, D. D. Agricultural pesticide use and risk of t(14;18)-defined subtypes of non-Hodgkin lymphoma. Blood. v. 108 n. 4. Washington, DC: American Society of Hematology,. p. 1363-1369.2006. COLOSSO C, Tiramani M &Maroni M.Neurobehavioral effects of pesticides: state of the art. Neurotoxicology24:577-591, 2003. CONAB, Companhia Nacional de Abastecimento. Acompanhamento da Safra Disponível:<http://www.conab.gov.br/OlalaCMS/uploads/arquivos/12_04_11_15_04_18_boletim_ abril_2012.pdf>. Acesso em: 13 jan, 2014, 21:56. CORREIA, F.L.A. Desenho e montagem de método rápido para diagnóstico da Borreliose de Lyme por PCR em tempo real. Tese de Mestrado, Universidade Fernando Pessoa. Porto, 70 pp.2007. DE OLIVEIRA HIRAGI, C.; MIRANDA-VILELA, A. L.; ROCHA, D. M. S.; DE OLIVEIRA, S. F. et al. Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups. Genetics and molecular biology, v. 34, n. 1, p. 11-18, 2011. DOLKEN, G.; DOLKEN, L.; HIRT, C.; FUSH, C.; RABKIN, C. S.; SCHULER, F. Agedependent prevalence and frequency of circulating t(14;18)-positive cells in the peripheral blood of healthy individuals. Journal of the National Cancer Institute Monographs.n. 39. Oxford: Oxford University Press, p. 44-47.2008. DUARTE RLM & PASCHOAL MEM. Marcadores moleculares no câncer de pulmão: papel prognóstico e sua relação com o tabagismo. J. Bras. Pneumol. 32 (1): 56-65.2005. DUELL, E. J.; HOLLY, E. A.; BRACCI, P. M.; LIU, M. et al. A population-based, case–control study of polymorphisms in carcinogen-metabolizing genes, smoking, and pancreatic adenocarcinoma risk. Journal of the National Cancer Institute, v. 94, n. 4, p. 297-306, 2002. ESPY, M.J., UHL, J.R., SLOAN, L.M., BUCKWALTER, S.P., JONES, M.F., VETTER, E.A., YAO, J.D.C., WENGENACK, N.L., ROSENBLATT, J.E., COCKERILL, F.R., SMITH, T.F. Real- Time PCR in clinical microbiology: applications for routine laboratory testing. Clinical Microbiology Reviews 19,165–256.2006. EVANS P.A.S., BASTARD C., DELABESSE E., MACINTYRE E.A., WIJERS- KOSTER P., SCHUURIG E., MOREAU E., GONZÁLEZ D., MILLS K.I., JENNINGS B.A., MILNER B.J., BLOXHAM D., STAROSTIK P., DELFAU-LARUE M.H., OTT M., SALLES G., MORGAN G.J.; t(14;18) with BCL2-IGH rearrangement. Leukemia 17, 2298-2301, 2003. FRESHNEY, R. I. Culture of Animal Cells: A Manual of Basic Technique. 4. ed. Nova York: Wiley-Liss, 397 p.1994. FUSCOE, J. C.; SETZER, R. W.; COLLARD, D. D.; MOORE, M. M. Quantification of t(14;18) in the lymphocytes of healthy adult humans as a possible biomarker for environmental exposure to carcinogens. Carcinogenesis. v. 17, n. 5. Oxford: Oxford University Press, p. 1013-1020.1996. GALL, J.G.; PARDUE, M.L. Formation and detection of RNADNA hybrid molecules in cytological preparations.Proceedings of the National Academy of Sciences of the United States of America, v.63, p.378-383, 1969. GARCIA, E. G. Segurança e saúde no trabalho rural: a questão dos agrotóxicos. 2001. GATEVA, S.; VARADINOVA, E.; GEORGIEVA, V. Genotoxic Activity of the Pesticide Devrinol in Human Lymphocytes in vitro.Comptes Rendus de l'Academie Bulgare des Sciences, v. 54, n. 2, p. 81, 2001. GILMAN, Goodman. As bases farmacológicas da terapêutica. 9. ed. Rio de Janeiro: MacGrawHill, 2006. GRIFFITHS, A. J. F.; LEWONTIN, R. C.; CARROLL S. B.; WESSLER S. R. Introdução à Genética. Guanabara Koogan, 10ª Ed. 2013 HARADA, S.; MISAWA, S.; NAKAMURA, T.; TANAKA, N. et al. Detection of GST1 gene deletion by the polymerase chain reaction and its possible correlation with stomach cancer in Japanese. Human genetics, v. 90, n. 1, p. 62-64, 1992. HATAGIMA, A.; KLAUTAU-GUIMARÃES, M. N.; SILVA, F. P.; CABELLO, P. H. Glutathione S-transferase M1 (GSTM1) polymorphism in two Brazilian populations. Genetics and molecular biology, v. 23, n. 4, p. 709-713, 2002. HEID. C.A., S., J., LIVAK, K.J., WILLIAMS, P.M.. Real time quantitative PCR. Genome Research 6, 986-994,1996. HIGUCHI, R., FOCKLER, C., DOLLINGER, G., WATSON, R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology Nature Publishing Company 11, 1026-1030.1993. HOLLAND, N., BOLOGNESI, C., KIRSCH-VOLDERS, M., BONASSI, S., ZEIGER, E., KNASMUELLER, S. & FENECH, M.The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps.Mutation Research,659(1-2):93-108, 2008. JACOBSON,L.S.V;HACON,S.S;ALVARENGA.L;GOLDSTEIN,R.A;GUMS,C;BUSS,D.F;LEDA ,L.R.Comunidade pomerana e uso de agrotóxicos:uma realidade pouco conhecida. Ciência e Saúde Coletiva,14(6): 2239-2249,2009. JONG D.; Molecular Pathogenesis of Follicular Lymphoma: A Cross Talk of Genetic and Immunologic Factors. Journal of Clinical Oncology, (23) 26: 6358-6363, 2005. KELADA, S. N.; KARDIA, S. L. R.; WALKER, A. H.; WEIN, A. J. et al. The Glutathione STransferase- μ and-θ Genotypes in the Etiology of Prostate Cancer: Genotype-Environment Interactions with Smoking. Cancer Epidemiology Biomarkers & Prevention, v. 9, n. 12, p. 1329-1334, 2000. KLUG, W. S.; CUMMINGS, M. R.; SPENCER, C. A.; PALLADINO, M. A. Conceitos de Genética – 9a Edição, Artmed, 2010. KUBISTA, M., ANDRADE, J.M., BENGTSSON, M., FOROOTAN, A., JONÁK, J.,LIND, K., SINDELKA, R., SJÖBACK, R., SJÖGREEN, B., STRÖMBOM, L., STÅHLBERG, A., ZORIC, N. The real- time polymerase chain reaction.Molecular Aspects of Medicine 27, 95-125, 2006. KUMAR, A.; YADAV, A.; GIRI, S. K.; DEV, K. et al. Allelic variation of GSTM1 and GSTT1 genes in Haryana population. Genomic Medicine, Biomarkers, and Health Sciences, 2012. KUMAR,R;DAS,B.C. Role os environmentalfactorsiscarcinogenesis.Environment & We an International Journal of Science & Technology, 4,53-59.2009. KVITKO, K.; BANDINELLI, E.; HENRIQUES, J. A. P.; HEUSER, V. D. et al. Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining. Genetics and molecular biology, v. 35, n. 4, p. 1060-1068, 2012. LEVSKY, J.M.; SINGER, R.H. Fluorescence in situ hybridization: past, present and Future. Journal of Cell Science, v.116, p.2833-2838, 2003. LUBIN, J. H.; BOICE JR, J. D. Lung cancer risk from residential radon: meta-analysis of eight epidemiologic studies. Journal of the National Cancer Institute, v. 89, n. 1, p. 49-57, 1997. MACHADO I.; ILLUECA C.; GARCÍA-CASADO Z.; LÓPEZ-GUERRERO J.; LORENTE P; LAVERNIA J. Linfoma de Hodgkin con presencia de t(14;18) en paciente con antecedentes de linfoma follicular. Revista Española de PatologíaVolume 46, Issue 2, April–Junep.106–111,2013. MACKAY, I. M., MACKAY, J.F., NISSEN, M.D., SLOOTS, T.P. Real-time PCR: History and Fluorogenic Chemistries in Mackay, p.1-40, I.M. (Ed.), Real-Time PCR in Microbiology, From Diagnosis to Characterization. Caister Academic Press Norfolk, UK.2007. MAPA, Ministério da Agricultura, Pecuária e Abastecimento. Brasil Projeções do Agronegócio 2010/2011 a 2020/2021, Assessoria De Gestão Estratégica Brasília, 2012. Disponível em<http://www.agricultura.gov.br/arq_editor/file/Ministerio/gestao/ projecao/PROJECOES%20DO%20AGRONEGOCIO%202010-11%20a%202020- 21%20- %202_0.pdf> Acesso em 10 jan,2014, 21:15 MASIR N, CAMPBELL LJ, GOFF LK, JONES M, MARAFIOTI T, CORDELL J, BCL2 protein expression in follicular lymphomas with t(14;18) chromosomal translocations. Br J Haematol.;144:716–25.2009. NAGEM, A. F. S. R.Dissertação: Avaliação Do Risco Ocupacional De Trabalhadores Expostos A Agrotóxicos No Município De Touros/Rio Grande Do Norte. Natal, 2013. NEVES S.M.N.; GUEDES R.M.C. Arq. Inst. Biol., São Paulo, v.79, n.4, p.627-632, out./dez., 2012 NIWA, O. Indirect mechanisms of genomic instability and the biological significance of mutations at tandem repeat loci. Mutation research. n. 598. Amsterdã: Elsevier,.p.61-72. 2006. NOVATO-SILVA. et al. A study of immunological alterations in rural workers laboriously exposed to pesticides.In: congresso mundial sobre segurança e saúde no trabalho, 15, São Paulo, 1999. OLIVEIRA, T. M. D. S. PCR em tempo real: métodos e aplicações. Departamento de Biologia, Universidade de Aveiro, 2010. OMS (Organização Mundial da Saúde). Public Health Impact of Pesticides Used in Agriculture.Genebra: The World Health Organization, 2010. PELT-VERKUIL, E., VAN BELKUM, A., HAYS, J.P. Principles and technical aspects of PCR amplification, Springer, 332 pp.2008. PERES, F.; MOREIRA, J. C. Saúde e ambiente em sua relação com o consumo de agrotóxicos em um pólo agrícola do Estado do Rio de Janeiro, Brasil Health, environment, andpesticide use in a farmingarea in Rio de Janeiro State, Brazil. Cad. Saúde Pública, v. 23, n. Sup 4, p. S612-S621, 2007. PIGNATI,W.A;MACHADO,J.M.H;CABRAL,J.F. Acidente rural ampliado: o caso das “chuvas” de agrotóxicos sobre a cidade de Lucas do Rio Verde – MT. Ciência-saúde coletiva,Rio de Janeiro.2007. PINHEL, M. A. S.; SADO, C. L.; LONGO, G. S.; GREGÓRIO, M. L.; AMORIM G. S.; FLORIM G. M. S.; MAZETI C. M.; MARTINS D. P.; OLIVEIRA, F. N.; NAKAZONE, M. A.; TOGNOLA W. A.; SOUZA D. R. S. Nullityof GSTT1/GSTM1 relatedtopesticidesisassociatedwithParkinson’sdisease. Arq Neuropsiquiatr 71(8):527-532, 2013. PLOEG, M. Cytochemical nucleic acid research during the twentieth century. European Journal of Histochemistry, v.44, p.7-42, 2000. RADIS Comunicação em Saúde, Agrotóxicos Proteção para quem? Manguinhos Rio de Janeiro,RJ No 95 Julho de 2010,Disponível em:<http://www4.ensp.fiocruz.br/radis/95/ pdf/radis_95.pdf> Acesso em 08 jan 2014, 22h38min. REIS, A. A. S. Estudo da associação do polimorfismo genético em carcinomas da tiróide. Revista de Biologia Neotropical, v. 7, n. 1, p. 61-62, 2011. RIBEIRO, L. R., Ed. Toxicologia GenéticaMutagenese Ambiental.: SBMCTA, v.1ª ediçao, 2003. RIBEIRO, M. L.; LOURENCETTI, C.; POLESE, L.; NAVICKIENE, S. et al. Pesticidas: Usos e Riscos para o Meio Ambiente. Holos Environment, v. 8, n. 1, p. 53-71, 2009. ROSSINI, A.; RAPOZO, D. C. M.; AMORIM, L. M. F.; MACEDO, J. M. B. et al. Frequencies of GSTM1, GSTT1, and GSTP1 polymorphisms in a Brazilian population. Genet Mol Res, v. 1, n. 3, p. 233-40, 2002. ROUISSI, K.; OUERHANI, S.; MARRAKCHI, R.; SLAMA, M. R. B. et al. Combined effect of smoking and inherited polymorphisms in arylamine N-acetyltransferase 2, glutathione Stransferases M1 and T1 on bladder cancer in a Tunisian population. Cancer genetics and cytogenetics, v. 190, n. 2, p. 101-107, 2009. ROULLAND, S.; LEBAILLY P.; ROUSSEL, G.; BRIAND M.; CAPPELLEN D.; POTTIER, D.; HARDOUIN, A.; TROUSSARD, X.; BASTARD, C.; HENRY-AMAR, M.; GAUDUCHON, P. BCL-2/JH translocation in peripheral blood lymphocytes of unexposed individuals: lack of seasonal variations in frequency and molecular features. International Journal of Cancer. v. 104. Heidelberg, Alemanha: German Cancer Research Center, 2003. SAFARINEJAD, M. R.; SAFARINEJAD, S.; SHAFIEI, N.; SAFARINEJAD, S. Association of genetic polymorphism of glutathione S-transferase (GSTM1, GSTT1, GSTP1) with bladder cancer susceptibility. Urologic oncology, 2011. SCHNEIDER, N. B. Avaliação de polimorfismo de genes de metabolização GSTT1, GSTM1, GSTP1 e PON1 na suscetibilidade individual a danos de DNA em sojicultores de Espumoso-RS. 2011. SCHÜLER, F.; HIRT, C.; DÖLKEN, G. Chromosomal translocation t(14;18) in healthy individuals. Seminars in Cancer Biology.v. 13. Amsterdã: Elsevier, 2003. SILVA, J. M. et al. Agrotóxico e trabalho: uma combinação perigosa para a saúde do trabalhador rural. Revista de Ciência e Saúde Coletiva, Rio de Janeiro, v. 10, n. 4, p. 891-903, 2005. SILVA.J. Genetic damage in soybean workers exposed to pesticides: evaluation with the comet and buccal micronucleus cytome assays.Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2013. SINDAG – Mercado de defensivos. Câmara Temática de Insumos Agropecuários Janeiro a outubro/2011.Disponívelem:<http://www.agricultura.gov.br/arq_editor/file/camaras_tematicas/Insu mos_agropecuari os/57RO/App_Defensivos.pdf Acesso em 08 jan, 2014, 21:46. SINGH, S.; KUMAR, V.; SINGH, P.; THAKUR, S. et al. Genetic polymorphisms of GSTM1, GSTT1 and GSTP1 and susceptibility to DNA damage in workers occupationally exposed to organophosphate pesticides. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, v. 725, n. 1–2, p. 36-42, 2011. SINITOX. Notificações de Intoxicações no Brasil - 2011. 2011. Disponível em: < http://www.fiocruz.br/sinitox_novo/cgi/cgilua.exe/sys/start.htm?sid=386>. Acesso em: 30/01/14. SOARES, W.; ALMEIDA, R. M. V. R; MORO, S. Trabalho rural e fatores de risco associados ao regime de uso de agrotóxicos em Minas Gerais, Brasil. Cadernos de Saúde Pública, Rio de Janeiro, v. 19, n. 4, p. 1117-1127, 2003. SOLDINI D., C. MONTAGNA, P. SCHÜFFLER, V. MARTIN, A. GEORGIS, T. THIESLER, A. CURIONI-FONTECEDRO, P. WENT, G. BOSSHARD, S. DEHLER, L. MAZZUCHELLI, AND M. TINGUELY. A new diagnostic algorithm for Burkitt and diffuse large B-cell lymphomas based on the expression of CSE1L and STAT3 and on MYC rearrangement predicts outcome.Ann Oncol (2013) 24 (1): 193-201 first published online September 11, 2012 SPRENGER, R; SCHLAGENHAUFER, R; KERB, R; BRUHN, C; BROCKMOLLER, J, ROOTS, I; BRINKMANN, U. Characterization of glutathione S-transferase GSTT1 deletion: discrimination of all genotypes by polymerase chain reaction indicates a trimodular genotype phenotype correlation. Pharmacogenetics; 10: 557-565.2000. SWINGER R.R.; TUCKER J.D. Fluorescence in situ hybridization: a brief review. Environmental and Molecular Mutagenesis, v.27, p.245-254, 1996. THOMPSON, J. S.; THOMPSON, M.W. Genética Médica.Elsevier, 2008. VERMA, R.S.; BABU, A. Human Chromosomes: Manual of Basic Techniques. São Paulo: Pergamon, 1989. VOLPI, E.V.; BRIDGER, J.M. FISH glossary: an overview of the fluorescence in situ hybridization technique. BioTechniques, v.45, p.385-409, 2008. WATSON, J. D. et al. Recombinant DNA: Genes and genomes: a short course. 3rd ed. New York: W. H. Freeman and Company, 2007. WESTMAN, J. A. Genética médica.Rio de Janeiro: Editora Guanabara Koogan, 2006. YASUKAWA, M.; BANDO, S.; DÖLKEN, G.; SADA, E.; YAKUSHIJIN, Y.; FUJITA, S.; MAKINO, H. Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy japanese individuals. Blood. v. 98. n. 2. Washington, DC: American Society of Hematology, 2001. YOUNG K.S. Clinical significance of cytogenetic aberrations in bone marrow of patients with diffuse large B-cell lymphoma: prognostic significance and relevance to histologic involvement.Journal of Hematology & Oncology 6, 2013. ZHANG, F., Immunophenotypic features and t(14;18) (q32;q21) translocation of Chinese follicular lymphomas helps to distinguish subgroups.Diagnostic Pathology 8, 154. 2013.-7235106986317239737600600600600-3872772117827373404-7683082515614575372075167498588264571http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPolimorfismosGSTTGSTMTranslocação 14;18AgrotóxicosIntoxicaçãoPolymorphismTranslocation 14;18AgrochemicalsPoisoningGSTT1GSTM1GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOSAvaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicosCytogenetic and molecular assessment of individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82142http://repositorio.bc.ufg.br/tede/bitstreams/4a9d8a70-0e19-4c67-8285-ca11b7e98614/download232e528055260031f4e2af4136033daaMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/dac69a4b-8f9a-48bf-95c7-f3e0d9501b1f/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822117http://repositorio.bc.ufg.br/tede/bitstreams/64b1edcf-c707-4eef-9fcc-f3bc197577cf/downloaddd6580d2d5007383f0e67b904850adc9MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.bc.ufg.br/tede/bitstreams/e1f126d2-95e6-4dbf-b290-99a96bcadc74/download9da0b6dfac957114c6a7714714b86306MD54ORIGINALBatista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdfBatista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdfDissertaçãoapplication/pdf1556018http://repositorio.bc.ufg.br/tede/bitstreams/0ee63b4a-c95c-49d8-ba04-dd2ba7140b4f/download89fbad76ace350a8cf9c7db5c92f015dMD55TEXTBatista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdf.txtBatista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdf.txtExtracted Texttext/plain101351http://repositorio.bc.ufg.br/tede/bitstreams/aa6484c4-1bcb-4bdd-82d4-043ff0186994/downloadf9e4a6acb07a06ffcff9a6fd119cbdebMD56THUMBNAILBatista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdf.jpgBatista, Mariana Pedrosa - Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos - 2014.pdf.jpgGenerated Thumbnailimage/jpeg2116http://repositorio.bc.ufg.br/tede/bitstreams/cfeb24f7-e435-443f-8df2-527e3f3fea10/downloadae44b72de53515029092bf0cbf233ea5MD57tde/30142014-09-19 09:13:28.356http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso abertoopen.accessoai:repositorio.bc.ufg.br:tde/3014http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-09-19T12:13:28Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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
dc.title.por.fl_str_mv Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
dc.title.alternative.eng.fl_str_mv Cytogenetic and molecular assessment of individuals
title Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
spellingShingle Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
Batista, Mariana Pedrosa
Polimorfismos
GSTT
GSTM
Translocação 14;18
Agrotóxicos
Intoxicação
Polymorphism
Translocation 14;18
Agrochemicals
Poisoning
GSTT1
GSTM1
GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
title_short Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
title_full Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
title_fullStr Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
title_full_unstemmed Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
title_sort Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos
author Batista, Mariana Pedrosa
author_facet Batista, Mariana Pedrosa
author_role author
dc.contributor.advisor1.fl_str_mv Silva, Daniela de Melo e
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9895211901348365
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2464432014109246
dc.contributor.author.fl_str_mv Batista, Mariana Pedrosa
contributor_str_mv Silva, Daniela de Melo e
dc.subject.por.fl_str_mv Polimorfismos
GSTT
GSTM
Translocação 14;18
Agrotóxicos
Intoxicação
topic Polimorfismos
GSTT
GSTM
Translocação 14;18
Agrotóxicos
Intoxicação
Polymorphism
Translocation 14;18
Agrochemicals
Poisoning
GSTT1
GSTM1
GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
dc.subject.eng.fl_str_mv Polymorphism
Translocation 14;18
Agrochemicals
Poisoning
GSTT1
GSTM1
dc.subject.cnpq.fl_str_mv GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
description Rural workers are constantly exposed to pesticides they use on crops, and this exposure may be responsible for genetic damage causing a health risk. A problem with the use of pesticides is the genotoxicity, which can lead to the onset of disease. Little is known about the relationship between genotoxicity and the variation of genetic polymorphisms of xenobiotic metabolism that may modify individual susceptibility to the possible genotoxic effects of pesticides. Therefore, there is a need to study genes as glutathione-S-transferase mu (GSTM1) and theta glutathione S-transferase (GSTT1) encoding detoxification enzymes of genotoxic compounds. Another important assessment in individuals exposed to pesticides is the presence of chromosomal translocation t(14;18) (q31,q21), which can be investigated at the molecular level. Thus, this study evaluated the polymorphisms of GSTM1 and GSTT1 genes in 120 individuals occupationally exposed to pesticides and 115 controls (without exposure to pesticides), by real-time PCR. The frequencies of GSTM1 and GSTT1 genotypes were found in 49 % and 18 %, respectively in the exposed group and 37 % and 18 %, respectively, for the control group. It has been found that there is a greater possibility of poisoning in workers who have null genotypes. There was no statistically significant correlation between the increased risk of intoxication and alcohol consumption, smoking and use of PPE. In addition, 29 workers exposed to pesticides for more than 15 years and with null genotypes in GSTT1 and / or GSTM1, were evaluated for the presence of the t (14;18)(q31, q21), in 100 nuclei, by fluorescent in situ hybridization (FISH) . The translocation was observed in only one individual, which may have been caused by prolonged exposure to pesticides, increasing age or alcohol consumption. Thus, the study of genetic polymorphisms and translocations as biomarkers of susceptibility is of fundamental importance in understanding the processes involved in genotype distribution mutagenesis and carcinogenesis and could help minimize the risks for susceptible individuals who are exposed to pesticides.
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-09-04T19:33:18Z
dc.date.available.fl_str_mv 2014-09-04
dc.date.issued.fl_str_mv 2014-02-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BATISTA, Mariana Pedrosa. Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos. 2014. 54 f. Dissertação (Mestrado em Genetica e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tde/3014
dc.identifier.dark.fl_str_mv ark:/38995/00130000083xz
identifier_str_mv BATISTA, Mariana Pedrosa. Avaliação citogenética e molecular de indivíduos ocupacionalmente expostos aos agrotóxicos. 2014. 54 f. Dissertação (Mestrado em Genetica e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.
ark:/38995/00130000083xz
url http://repositorio.bc.ufg.br/tede/handle/tde/3014
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv -7235106986317239737
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -3872772117827373404
dc.relation.cnpq.fl_str_mv -768308251561457537
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.relation.references.por.fl_str_mv ABBAS, A.; DELVINQUIÈRE, K.; LECHEVREL, M.; LEBAILLY, P. et al. GSTM1, GSTT1, GSTP1 and CYP1A1 genetic polymorphisms and susceptibility to esophageal cancer in a French population: different pattern of squamous cell carcinoma and adenocarcinoma. World J Gastroenterol, v. 10, n. 23, p. 3389-93, 2004. ABDEL-RAHMAN SZ.; EL-ZEIN RA, ANWAR WA.; AU WW. A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies. Cancer Lett.; 10: 229- 233,1996. ABHISHEK, S.; KAUR, N.; KAUR, S.; LATA, M. et al. Association of GSTM1 and GSTT1 gene deletions with susceptibility to DNA damage in the pesticide-exposed workers of Punjab. Rejuvenation Research, v. 13, n. 2-3, p. 281-284, 2010. ABRASCO, Associação Brasileira de Saúde Coletiva. DOSSIÊ ABRASCO – Uma alerta sobre os impactos dos Agrotóxicos na Saúde ABRASCO, Rio de Janeiro, abril de 2012, 1a Parte.98p. Disponível em:<http://www.abrasco.org.br/UserFiles/File ABRASCODIVULGA/2012/DossieAGT.pdf>. Acesso em : 13 de jan,2014, 20:50. ALAVANJA M.C.R, HOPPIN J.A. & KAMEL F.Health effects of chronic pesticide exposure: cancer and neurotoxity. Annu. Rev. Public Health 25:157-197,2004. ANGERER, J.; EWERS, U.; WILHELM, M. Human biomonitoring: state of the art. International journal of hygiene and environmental health, v. 210, n. 3-4, p. 201-228, 2007. ANVISA. Agrotóxico e toxicologia. Site ANVISA, 2011. Disponível em: < http://portal.anvisa.gov.br/wps/portal/anvisa/anvisa/home/agrotoxicotoxicologia >. Acesso em: 08/01/14 ARRUDA, V.R.; GRIGNOLI, C.E.; GONÇALVES, Prevalence of homozygosity for the deleted alleles of glutathione S-transferase mu (GSTM1) and theta (GSTT1) among distinct ethnic groups from Brazil: relevance to environmental carcinogenesis? Clinical Genetics,54:210-214,1998. AYRES M, AYRES JR. M, AYRES DL, SANTOS AS. BioEstat: aplicações estatísticas nas áreas das ciências bio-médicas. Belém: Sociedade Civil Mamirauá; 132-214 p.2007. BAUMAN, J.G.J.; WIEGANT, J.; BORST, P.; DUIJN, P.A new method for microscopial localization of specific DNA sequences by in situ hybridization of fluorchrome-labelled RNA. Experimental Cell Research, v.128, p.485- 490, 1980. BELL, D. A., LIU, Y., CORTOPASSI, G. A. Occurrence of bcl-2 oncogene translocation with increased frequency in the peripheral blood of heavy smokers.J. Natl Cancer Inst. n. 87. p. 223– 224,1995. BENEDETTI.D, NUNES.E, SARMENTO.M, PORTO.C, DIAS.J.F, SANTOS.C.E.I,BLAIR, A.; ZAHM S. H.; PEARCE, N. E.; HEINEMAN, E. F.; FRAUMENI, J. F. Clues to cancer etiology from studies of farmers. Scandinavian Journal of Work, Environment & Health. n. 18. Helsinque, p. 209-215,1992. BOCHNER, R. Sistema Nacional de Informações Tóxico-Farmacológicas–SINITOX e as intoxicações humanas por agrotóxicos no Brasil. Cien Saude Colet, v. 12, n. 1, p. 73-89, 2007. BOLOGNESI, C. Genotoxicity of pesticides: a review of human biomonitoring studies. Mutation Research/Reviews in Mutation Research, v. 543, n. 3, p. 251-272, 2003. BRASIL, Ministério da Saúde. Secretaria de Vigilância Sanitária. Manual de Vigilância da Saúde de Populações Expostas a Agrotóxicos. Organização Pan-Americana de Saúde/Organização Mundial de Saúde. Brasília.1997. BUCHARD, A.; SANCHEZ, J. J.; DALHOFF, K.; MORLING, N.et al. Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants: Simultaneously Detecting GSTM1 and GSTT1 Gene Copy Number and the Allelic Status of the GSTP1 Ile105Val Genetic Variant. The Journal of molecular diagnostics: JMD, v. 9, n. 5, p. 612, 2007. BUFALO NE, LEITE JL, GUILHEN AC, MORARI EC, GRANJA F, ASSUMPCAO LV, WARD LS. Smoking and susceptibility to thyroid cancer: an inverse association with CYP1A1 allelic variants. Endocr Relat Cancer.; 13(4):1185-93,2006. BUGANO, D.; CONFORTI-FROES, N.; YAMAGUCHI, N.; BARACAT, E. Genetic polymorphisms, the metabolism of estrogens and breast cancer: a review. European journal of gynaecological oncology, v. 29, n. 4, p. 313, 2008. BULL, S.; FLETCHER, K.; BOOBIS, A.; BATTERSHILL, J. Evidence for genotoxicity of pesticides in pesticide applicators: a review. Mutagenesis, v. 21, n. 2, p. 93-103, 2006. BURIM, R. V.; CANALLE, R.; MARTINELLI, A. L. C.; TAKAHASHI, C. S. Polymorphisms in glutathione S_transferases GSTM1, GSTT1 and GSTP1 and cytochromes P450 CYP2E1 and CYP1A1 and susceptibility to cirrhosis or pancreatitis in alcoholics. Mutagenesis, v. 19, n. 4, p. 291-298, 2004. BUSTILLOS, N. T.; ASCARRUNZ, M. E.; AGUILAR, X.; RADA, A. Polimorfismos genéticos de la GSTM1 y GSTT1 como modificadores de riesgo mutagénico en agricultores bolivianos expuestos a plaguicidas. BIOFARBO, v. 20, n. 1, 2012. BUTLER, M. W.; HACKETT, N. R.; SALIT, J.; STRULOVICI-BAREL, Y. et al. Glutathione Stransferase copy number variation alters lung gene expression. European Respiratory Journal, v. 38, n. 1, p. 15-28, 2011. CALDERÓN-SEGURA, M. E.; GÓMEZ-ARROYO, S.; VILLALOBOS-PIETRINI, R.; MARTÍNEZ-VALENZUELA, C. et al. Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes Exposed In Vitro to Neonicotinoid Insecticides News. Journal of Toxicology, v. 2012, 2012. CARNEIRO, F. F. P., W; RIGOTTO, R M; AUGUSTO, L G S. RIZZOLO, A; MULLER, N M; ALEXANDRE, V P.; FRIEDRICH, K; MELLO, M S C. Dossiê ABRASCO – Um alerta sobre os impactos dos agrotóxicos na saúde. Parte 1 - Agrotóxicos, Segurança Alimentar e Nutricional e Saúde. Rio de Janeiro: ABRASCO, 2012. CARVALHO, C.A.M.C. Aplicação médico-legal da PCR em tempo real na caracterização de SNPs. Tese de Mestrado, Faculdade de Medicina da Universidade do Porto. Porto, 75 pp.2007. CERRONI L., WIESNER T.. Linfomas cutáneos: de lamorfología a latecnología de microprocesador. ActasDermo-Sifiliográficas Volume 100, Supplement 1, November, p.3–17, 2009. CHANG XIN S., L.H., LIN X., YIRONG L. The detection of circulating tumor cells of breast cancer patients by using multimarker (Survivin, hTERT and hMAM) quantitative real-time PCR.Clinical Biochemistry 42, 194-200, (2010). CHAVES, T. V. S. Dissertação: Avaliação do impacto do uso de agrotóxicos nos trabalhadores rurais dos municípios de ribeiro gonçalves, baixa grande do ribeiro e uruçuí – piauí. Fortaleza, 2007 CHIU, B. C.; DAVE, B. J.; BLAIR, A.; GAPSTUR, S. M.; ZAHM, S. H.; WEISENBURGER, D. D. Agricultural pesticide use and risk of t(14;18)-defined subtypes of non-Hodgkin lymphoma. Blood. v. 108 n. 4. Washington, DC: American Society of Hematology,. p. 1363-1369.2006. COLOSSO C, Tiramani M &Maroni M.Neurobehavioral effects of pesticides: state of the art. Neurotoxicology24:577-591, 2003. CONAB, Companhia Nacional de Abastecimento. Acompanhamento da Safra Disponível:<http://www.conab.gov.br/OlalaCMS/uploads/arquivos/12_04_11_15_04_18_boletim_ abril_2012.pdf>. Acesso em: 13 jan, 2014, 21:56. CORREIA, F.L.A. Desenho e montagem de método rápido para diagnóstico da Borreliose de Lyme por PCR em tempo real. Tese de Mestrado, Universidade Fernando Pessoa. Porto, 70 pp.2007. DE OLIVEIRA HIRAGI, C.; MIRANDA-VILELA, A. L.; ROCHA, D. M. S.; DE OLIVEIRA, S. F. et al. Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups. Genetics and molecular biology, v. 34, n. 1, p. 11-18, 2011. DOLKEN, G.; DOLKEN, L.; HIRT, C.; FUSH, C.; RABKIN, C. S.; SCHULER, F. Agedependent prevalence and frequency of circulating t(14;18)-positive cells in the peripheral blood of healthy individuals. Journal of the National Cancer Institute Monographs.n. 39. Oxford: Oxford University Press, p. 44-47.2008. DUARTE RLM & PASCHOAL MEM. Marcadores moleculares no câncer de pulmão: papel prognóstico e sua relação com o tabagismo. J. Bras. Pneumol. 32 (1): 56-65.2005. DUELL, E. J.; HOLLY, E. A.; BRACCI, P. M.; LIU, M. et al. A population-based, case–control study of polymorphisms in carcinogen-metabolizing genes, smoking, and pancreatic adenocarcinoma risk. Journal of the National Cancer Institute, v. 94, n. 4, p. 297-306, 2002. ESPY, M.J., UHL, J.R., SLOAN, L.M., BUCKWALTER, S.P., JONES, M.F., VETTER, E.A., YAO, J.D.C., WENGENACK, N.L., ROSENBLATT, J.E., COCKERILL, F.R., SMITH, T.F. Real- Time PCR in clinical microbiology: applications for routine laboratory testing. Clinical Microbiology Reviews 19,165–256.2006. EVANS P.A.S., BASTARD C., DELABESSE E., MACINTYRE E.A., WIJERS- KOSTER P., SCHUURIG E., MOREAU E., GONZÁLEZ D., MILLS K.I., JENNINGS B.A., MILNER B.J., BLOXHAM D., STAROSTIK P., DELFAU-LARUE M.H., OTT M., SALLES G., MORGAN G.J.; t(14;18) with BCL2-IGH rearrangement. Leukemia 17, 2298-2301, 2003. FRESHNEY, R. I. Culture of Animal Cells: A Manual of Basic Technique. 4. ed. Nova York: Wiley-Liss, 397 p.1994. FUSCOE, J. C.; SETZER, R. W.; COLLARD, D. D.; MOORE, M. M. Quantification of t(14;18) in the lymphocytes of healthy adult humans as a possible biomarker for environmental exposure to carcinogens. Carcinogenesis. v. 17, n. 5. Oxford: Oxford University Press, p. 1013-1020.1996. GALL, J.G.; PARDUE, M.L. Formation and detection of RNADNA hybrid molecules in cytological preparations.Proceedings of the National Academy of Sciences of the United States of America, v.63, p.378-383, 1969. GARCIA, E. G. Segurança e saúde no trabalho rural: a questão dos agrotóxicos. 2001. GATEVA, S.; VARADINOVA, E.; GEORGIEVA, V. Genotoxic Activity of the Pesticide Devrinol in Human Lymphocytes in vitro.Comptes Rendus de l'Academie Bulgare des Sciences, v. 54, n. 2, p. 81, 2001. GILMAN, Goodman. As bases farmacológicas da terapêutica. 9. ed. Rio de Janeiro: MacGrawHill, 2006. GRIFFITHS, A. J. F.; LEWONTIN, R. C.; CARROLL S. B.; WESSLER S. R. Introdução à Genética. Guanabara Koogan, 10ª Ed. 2013 HARADA, S.; MISAWA, S.; NAKAMURA, T.; TANAKA, N. et al. Detection of GST1 gene deletion by the polymerase chain reaction and its possible correlation with stomach cancer in Japanese. Human genetics, v. 90, n. 1, p. 62-64, 1992. HATAGIMA, A.; KLAUTAU-GUIMARÃES, M. N.; SILVA, F. P.; CABELLO, P. H. Glutathione S-transferase M1 (GSTM1) polymorphism in two Brazilian populations. Genetics and molecular biology, v. 23, n. 4, p. 709-713, 2002. HEID. C.A., S., J., LIVAK, K.J., WILLIAMS, P.M.. Real time quantitative PCR. Genome Research 6, 986-994,1996. HIGUCHI, R., FOCKLER, C., DOLLINGER, G., WATSON, R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology Nature Publishing Company 11, 1026-1030.1993. HOLLAND, N., BOLOGNESI, C., KIRSCH-VOLDERS, M., BONASSI, S., ZEIGER, E., KNASMUELLER, S. & FENECH, M.The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps.Mutation Research,659(1-2):93-108, 2008. JACOBSON,L.S.V;HACON,S.S;ALVARENGA.L;GOLDSTEIN,R.A;GUMS,C;BUSS,D.F;LEDA ,L.R.Comunidade pomerana e uso de agrotóxicos:uma realidade pouco conhecida. Ciência e Saúde Coletiva,14(6): 2239-2249,2009. JONG D.; Molecular Pathogenesis of Follicular Lymphoma: A Cross Talk of Genetic and Immunologic Factors. Journal of Clinical Oncology, (23) 26: 6358-6363, 2005. KELADA, S. N.; KARDIA, S. L. R.; WALKER, A. H.; WEIN, A. J. et al. The Glutathione STransferase- μ and-θ Genotypes in the Etiology of Prostate Cancer: Genotype-Environment Interactions with Smoking. Cancer Epidemiology Biomarkers & Prevention, v. 9, n. 12, p. 1329-1334, 2000. KLUG, W. S.; CUMMINGS, M. R.; SPENCER, C. A.; PALLADINO, M. A. Conceitos de Genética – 9a Edição, Artmed, 2010. KUBISTA, M., ANDRADE, J.M., BENGTSSON, M., FOROOTAN, A., JONÁK, J.,LIND, K., SINDELKA, R., SJÖBACK, R., SJÖGREEN, B., STRÖMBOM, L., STÅHLBERG, A., ZORIC, N. The real- time polymerase chain reaction.Molecular Aspects of Medicine 27, 95-125, 2006. KUMAR, A.; YADAV, A.; GIRI, S. K.; DEV, K. et al. Allelic variation of GSTM1 and GSTT1 genes in Haryana population. Genomic Medicine, Biomarkers, and Health Sciences, 2012. KUMAR,R;DAS,B.C. Role os environmentalfactorsiscarcinogenesis.Environment & We an International Journal of Science & Technology, 4,53-59.2009. KVITKO, K.; BANDINELLI, E.; HENRIQUES, J. A. P.; HEUSER, V. D. et al. Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining. Genetics and molecular biology, v. 35, n. 4, p. 1060-1068, 2012. LEVSKY, J.M.; SINGER, R.H. Fluorescence in situ hybridization: past, present and Future. Journal of Cell Science, v.116, p.2833-2838, 2003. LUBIN, J. H.; BOICE JR, J. D. Lung cancer risk from residential radon: meta-analysis of eight epidemiologic studies. Journal of the National Cancer Institute, v. 89, n. 1, p. 49-57, 1997. MACHADO I.; ILLUECA C.; GARCÍA-CASADO Z.; LÓPEZ-GUERRERO J.; LORENTE P; LAVERNIA J. Linfoma de Hodgkin con presencia de t(14;18) en paciente con antecedentes de linfoma follicular. Revista Española de PatologíaVolume 46, Issue 2, April–Junep.106–111,2013. MACKAY, I. M., MACKAY, J.F., NISSEN, M.D., SLOOTS, T.P. Real-time PCR: History and Fluorogenic Chemistries in Mackay, p.1-40, I.M. (Ed.), Real-Time PCR in Microbiology, From Diagnosis to Characterization. Caister Academic Press Norfolk, UK.2007. MAPA, Ministério da Agricultura, Pecuária e Abastecimento. Brasil Projeções do Agronegócio 2010/2011 a 2020/2021, Assessoria De Gestão Estratégica Brasília, 2012. Disponível em<http://www.agricultura.gov.br/arq_editor/file/Ministerio/gestao/ projecao/PROJECOES%20DO%20AGRONEGOCIO%202010-11%20a%202020- 21%20- %202_0.pdf> Acesso em 10 jan,2014, 21:15 MASIR N, CAMPBELL LJ, GOFF LK, JONES M, MARAFIOTI T, CORDELL J, BCL2 protein expression in follicular lymphomas with t(14;18) chromosomal translocations. Br J Haematol.;144:716–25.2009. NAGEM, A. F. S. R.Dissertação: Avaliação Do Risco Ocupacional De Trabalhadores Expostos A Agrotóxicos No Município De Touros/Rio Grande Do Norte. Natal, 2013. NEVES S.M.N.; GUEDES R.M.C. Arq. Inst. Biol., São Paulo, v.79, n.4, p.627-632, out./dez., 2012 NIWA, O. Indirect mechanisms of genomic instability and the biological significance of mutations at tandem repeat loci. Mutation research. n. 598. Amsterdã: Elsevier,.p.61-72. 2006. NOVATO-SILVA. et al. A study of immunological alterations in rural workers laboriously exposed to pesticides.In: congresso mundial sobre segurança e saúde no trabalho, 15, São Paulo, 1999. OLIVEIRA, T. M. D. S. PCR em tempo real: métodos e aplicações. Departamento de Biologia, Universidade de Aveiro, 2010. OMS (Organização Mundial da Saúde). Public Health Impact of Pesticides Used in Agriculture.Genebra: The World Health Organization, 2010. PELT-VERKUIL, E., VAN BELKUM, A., HAYS, J.P. Principles and technical aspects of PCR amplification, Springer, 332 pp.2008. PERES, F.; MOREIRA, J. C. Saúde e ambiente em sua relação com o consumo de agrotóxicos em um pólo agrícola do Estado do Rio de Janeiro, Brasil Health, environment, andpesticide use in a farmingarea in Rio de Janeiro State, Brazil. Cad. Saúde Pública, v. 23, n. Sup 4, p. S612-S621, 2007. PIGNATI,W.A;MACHADO,J.M.H;CABRAL,J.F. Acidente rural ampliado: o caso das “chuvas” de agrotóxicos sobre a cidade de Lucas do Rio Verde – MT. Ciência-saúde coletiva,Rio de Janeiro.2007. PINHEL, M. A. S.; SADO, C. L.; LONGO, G. S.; GREGÓRIO, M. L.; AMORIM G. S.; FLORIM G. M. S.; MAZETI C. M.; MARTINS D. P.; OLIVEIRA, F. N.; NAKAZONE, M. A.; TOGNOLA W. A.; SOUZA D. R. S. Nullityof GSTT1/GSTM1 relatedtopesticidesisassociatedwithParkinson’sdisease. Arq Neuropsiquiatr 71(8):527-532, 2013. PLOEG, M. Cytochemical nucleic acid research during the twentieth century. European Journal of Histochemistry, v.44, p.7-42, 2000. RADIS Comunicação em Saúde, Agrotóxicos Proteção para quem? Manguinhos Rio de Janeiro,RJ No 95 Julho de 2010,Disponível em:<http://www4.ensp.fiocruz.br/radis/95/ pdf/radis_95.pdf> Acesso em 08 jan 2014, 22h38min. REIS, A. A. S. Estudo da associação do polimorfismo genético em carcinomas da tiróide. Revista de Biologia Neotropical, v. 7, n. 1, p. 61-62, 2011. RIBEIRO, L. R., Ed. Toxicologia GenéticaMutagenese Ambiental.: SBMCTA, v.1ª ediçao, 2003. RIBEIRO, M. L.; LOURENCETTI, C.; POLESE, L.; NAVICKIENE, S. et al. Pesticidas: Usos e Riscos para o Meio Ambiente. Holos Environment, v. 8, n. 1, p. 53-71, 2009. ROSSINI, A.; RAPOZO, D. C. M.; AMORIM, L. M. F.; MACEDO, J. M. B. et al. Frequencies of GSTM1, GSTT1, and GSTP1 polymorphisms in a Brazilian population. Genet Mol Res, v. 1, n. 3, p. 233-40, 2002. ROUISSI, K.; OUERHANI, S.; MARRAKCHI, R.; SLAMA, M. R. B. et al. Combined effect of smoking and inherited polymorphisms in arylamine N-acetyltransferase 2, glutathione Stransferases M1 and T1 on bladder cancer in a Tunisian population. Cancer genetics and cytogenetics, v. 190, n. 2, p. 101-107, 2009. ROULLAND, S.; LEBAILLY P.; ROUSSEL, G.; BRIAND M.; CAPPELLEN D.; POTTIER, D.; HARDOUIN, A.; TROUSSARD, X.; BASTARD, C.; HENRY-AMAR, M.; GAUDUCHON, P. BCL-2/JH translocation in peripheral blood lymphocytes of unexposed individuals: lack of seasonal variations in frequency and molecular features. International Journal of Cancer. v. 104. Heidelberg, Alemanha: German Cancer Research Center, 2003. SAFARINEJAD, M. R.; SAFARINEJAD, S.; SHAFIEI, N.; SAFARINEJAD, S. Association of genetic polymorphism of glutathione S-transferase (GSTM1, GSTT1, GSTP1) with bladder cancer susceptibility. Urologic oncology, 2011. SCHNEIDER, N. B. Avaliação de polimorfismo de genes de metabolização GSTT1, GSTM1, GSTP1 e PON1 na suscetibilidade individual a danos de DNA em sojicultores de Espumoso-RS. 2011. SCHÜLER, F.; HIRT, C.; DÖLKEN, G. Chromosomal translocation t(14;18) in healthy individuals. Seminars in Cancer Biology.v. 13. Amsterdã: Elsevier, 2003. SILVA, J. M. et al. Agrotóxico e trabalho: uma combinação perigosa para a saúde do trabalhador rural. Revista de Ciência e Saúde Coletiva, Rio de Janeiro, v. 10, n. 4, p. 891-903, 2005. SILVA.J. Genetic damage in soybean workers exposed to pesticides: evaluation with the comet and buccal micronucleus cytome assays.Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2013. SINDAG – Mercado de defensivos. Câmara Temática de Insumos Agropecuários Janeiro a outubro/2011.Disponívelem:<http://www.agricultura.gov.br/arq_editor/file/camaras_tematicas/Insu mos_agropecuari os/57RO/App_Defensivos.pdf Acesso em 08 jan, 2014, 21:46. SINGH, S.; KUMAR, V.; SINGH, P.; THAKUR, S. et al. Genetic polymorphisms of GSTM1, GSTT1 and GSTP1 and susceptibility to DNA damage in workers occupationally exposed to organophosphate pesticides. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, v. 725, n. 1–2, p. 36-42, 2011. SINITOX. Notificações de Intoxicações no Brasil - 2011. 2011. Disponível em: < http://www.fiocruz.br/sinitox_novo/cgi/cgilua.exe/sys/start.htm?sid=386>. Acesso em: 30/01/14. SOARES, W.; ALMEIDA, R. M. V. R; MORO, S. Trabalho rural e fatores de risco associados ao regime de uso de agrotóxicos em Minas Gerais, Brasil. Cadernos de Saúde Pública, Rio de Janeiro, v. 19, n. 4, p. 1117-1127, 2003. SOLDINI D., C. MONTAGNA, P. SCHÜFFLER, V. MARTIN, A. GEORGIS, T. THIESLER, A. CURIONI-FONTECEDRO, P. WENT, G. BOSSHARD, S. DEHLER, L. MAZZUCHELLI, AND M. TINGUELY. A new diagnostic algorithm for Burkitt and diffuse large B-cell lymphomas based on the expression of CSE1L and STAT3 and on MYC rearrangement predicts outcome.Ann Oncol (2013) 24 (1): 193-201 first published online September 11, 2012 SPRENGER, R; SCHLAGENHAUFER, R; KERB, R; BRUHN, C; BROCKMOLLER, J, ROOTS, I; BRINKMANN, U. Characterization of glutathione S-transferase GSTT1 deletion: discrimination of all genotypes by polymerase chain reaction indicates a trimodular genotype phenotype correlation. Pharmacogenetics; 10: 557-565.2000. SWINGER R.R.; TUCKER J.D. Fluorescence in situ hybridization: a brief review. Environmental and Molecular Mutagenesis, v.27, p.245-254, 1996. THOMPSON, J. S.; THOMPSON, M.W. Genética Médica.Elsevier, 2008. VERMA, R.S.; BABU, A. Human Chromosomes: Manual of Basic Techniques. São Paulo: Pergamon, 1989. VOLPI, E.V.; BRIDGER, J.M. FISH glossary: an overview of the fluorescence in situ hybridization technique. BioTechniques, v.45, p.385-409, 2008. WATSON, J. D. et al. Recombinant DNA: Genes and genomes: a short course. 3rd ed. New York: W. H. Freeman and Company, 2007. WESTMAN, J. A. Genética médica.Rio de Janeiro: Editora Guanabara Koogan, 2006. YASUKAWA, M.; BANDO, S.; DÖLKEN, G.; SADA, E.; YAKUSHIJIN, Y.; FUJITA, S.; MAKINO, H. Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy japanese individuals. Blood. v. 98. n. 2. Washington, DC: American Society of Hematology, 2001. YOUNG K.S. Clinical significance of cytogenetic aberrations in bone marrow of patients with diffuse large B-cell lymphoma: prognostic significance and relevance to histologic involvement.Journal of Hematology & Oncology 6, 2013. ZHANG, F., Immunophenotypic features and t(14;18) (q32;q21) translocation of Chinese follicular lymphomas helps to distinguish subgroups.Diagnostic Pathology 8, 154. 2013.
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Genetica e Biologia Molecular
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ciências Biológicas - ICB (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Repositório Institucional da UFG
collection Repositório Institucional da UFG
bitstream.url.fl_str_mv http://repositorio.bc.ufg.br/tede/bitstreams/4a9d8a70-0e19-4c67-8285-ca11b7e98614/download
http://repositorio.bc.ufg.br/tede/bitstreams/dac69a4b-8f9a-48bf-95c7-f3e0d9501b1f/download
http://repositorio.bc.ufg.br/tede/bitstreams/64b1edcf-c707-4eef-9fcc-f3bc197577cf/download
http://repositorio.bc.ufg.br/tede/bitstreams/e1f126d2-95e6-4dbf-b290-99a96bcadc74/download
http://repositorio.bc.ufg.br/tede/bitstreams/0ee63b4a-c95c-49d8-ba04-dd2ba7140b4f/download
http://repositorio.bc.ufg.br/tede/bitstreams/aa6484c4-1bcb-4bdd-82d4-043ff0186994/download
http://repositorio.bc.ufg.br/tede/bitstreams/cfeb24f7-e435-443f-8df2-527e3f3fea10/download
bitstream.checksum.fl_str_mv 232e528055260031f4e2af4136033daa
4afdbb8c545fd630ea7db775da747b2f
dd6580d2d5007383f0e67b904850adc9
9da0b6dfac957114c6a7714714b86306
89fbad76ace350a8cf9c7db5c92f015d
f9e4a6acb07a06ffcff9a6fd119cbdeb
ae44b72de53515029092bf0cbf233ea5
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
_version_ 1815172596415594496

Registros relacionados