Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/11160 |
Resumo: | This work describes the development and characterization of Magnetosomes Paclitaxel loaded (MS PTX-loaded) for cutaneous applications in magnetohyperthermia. Magnetic nanoparticles (MP) were prepared by coprecipitation of Fe (II) and Fe (III) or Mn (II) and Fe (III) salts in alkaline medium. MS were produced by hydration method of a preformed lipid film. The nanostructured system was characterized in terms of morphology, mean diameter and size distribution, encapsulation efficiency of PTX, stability and magnetic properties of magnetometry and magnetohyperthermia. MS PTX-loading had an average diameter of approximately 90nm with polydispersion index of 0.190. The PTX: lipid ratio was 1:50 (m/m) with 81% PTX encapsulation efficiency. Stability study of lyophilized MS PTX-loading showed 98% of the encapsulation efficiency for 60 days. MS PTX-loading presented superparamagnetic behavior in the presence of magnetic field, with saturation magnetization of 0.25 emu/g and volumetric fraction of 11.3x10-3. The concentration of MP in the formulation was 5.56mg/mL. The effect of magnetohyperthermia with variation of temperature of up to 30ºC was verified from the application of alternating magnetic field. This increase in MS temperature influenced the rate of in vitro release of the encapsulated PTX, allowing an increase of 400% release comparing temperatures of 25ºC and 43ºC. Thus, through the in vitro release study it was possible to perceive that magnetohyperthermia could act as a release trigger of PTX encapsulated in MS. From the biological assay of cytotoxicity with B16F10 cells it was possible to verify the cellular viability of the nanosystem and to determine the IC50. The application of a magnetic field together with the release of PTX showed a great potential for the cytotoxic action under the B16F10 strain by the impossibility of approximately 100% of the cells in a period of 1.5h. |
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Lima, Eliana Martinshttp://lattes.cnpq.br/7248774319455970Lima, Eliana MartinsSantos, Marcus CarriãoCastro, Elisandra Gava deRahal, Rosemar Macedo de SouzaNascimento, Thais Leitehttp://lattes.cnpq.br/4031577881091710Oliveira, Relton Romeis de2021-03-17T12:07:03Z2021-03-17T12:07:03Z2018-10-10OLIVEIRA, R. R. Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia. 2020. 106 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/11160This work describes the development and characterization of Magnetosomes Paclitaxel loaded (MS PTX-loaded) for cutaneous applications in magnetohyperthermia. Magnetic nanoparticles (MP) were prepared by coprecipitation of Fe (II) and Fe (III) or Mn (II) and Fe (III) salts in alkaline medium. MS were produced by hydration method of a preformed lipid film. The nanostructured system was characterized in terms of morphology, mean diameter and size distribution, encapsulation efficiency of PTX, stability and magnetic properties of magnetometry and magnetohyperthermia. MS PTX-loading had an average diameter of approximately 90nm with polydispersion index of 0.190. The PTX: lipid ratio was 1:50 (m/m) with 81% PTX encapsulation efficiency. Stability study of lyophilized MS PTX-loading showed 98% of the encapsulation efficiency for 60 days. MS PTX-loading presented superparamagnetic behavior in the presence of magnetic field, with saturation magnetization of 0.25 emu/g and volumetric fraction of 11.3x10-3. The concentration of MP in the formulation was 5.56mg/mL. The effect of magnetohyperthermia with variation of temperature of up to 30ºC was verified from the application of alternating magnetic field. This increase in MS temperature influenced the rate of in vitro release of the encapsulated PTX, allowing an increase of 400% release comparing temperatures of 25ºC and 43ºC. Thus, through the in vitro release study it was possible to perceive that magnetohyperthermia could act as a release trigger of PTX encapsulated in MS. From the biological assay of cytotoxicity with B16F10 cells it was possible to verify the cellular viability of the nanosystem and to determine the IC50. The application of a magnetic field together with the release of PTX showed a great potential for the cytotoxic action under the B16F10 strain by the impossibility of approximately 100% of the cells in a period of 1.5h.Este trabalho descreve o desenvolvimento e caracterização de magnetossomas (MS) contendo Paclitaxel (PTX) para aplicação cutânea em magnetohipertermia. Nanopartículas magnéticas (PM) foram obtidas pelo método de co-precipitação de sais de Fe(II) e Fe(III) ou Mn(II) e Fe(III) em meio alcalino. MS foram produzidos pelo método de hidratação de um filme lipídico pré-formado. O sistema nanoestruturado foi caracterizado quanto à morfologia, diâmetro médio e distribuição de tamanho, eficiência de encapsulação do PTX, estabilidade e propriedades magnéticas por magnetometria e magnetohipertermia. MS contendo PTX apresentaram diâmetro médio de aproximadamente 90nm com índice de polidispersão de 0,190. A relação PTX: lipídeo foi de 1:50 (m/m) com eficiência de encapsulação de PTX de 81%. Estudo de estabilidade de MS liofilizados contendo PTX apresentaram 98% de estabilidade da eficiência de encapsulação por 60 dias. MS contendo PTX apresentaram comportamento superparamagnético na presença de campo, com magnetização de saturação de 0,25 emu/g e fração volumétrica de 11,3x10-3. A concentração de PM na formulação foi de 5,56mg/mL. O efeito de magnetohipertermia com variação de temperatura de até 30ºC foi verificado a partir da aplicação de campo magnético alternado. Esse aumento da temperatura dos MS influenciou na taxa de liberação in vitro do PTX encapsulado, possibilitando um aumento da liberação em 400% comparando temperaturas de 25ºC e 43ºC. Desta forma, através do estudo de liberação in vitro foi possível perceber que a magnetohipertermia pode funcionar como gatilho de liberação do PTX encapsulado em MS. A partir de ensaio biológico de citotoxicidade com células B16F10 foi possível verificar a viabilidade celular do nanossistema e determinar o IC50. E a aplicação de campo magnético conjunta à liberação do PTX mostrou grande potencial para a ação citotóxica sob a linhagem de melanoma murino B16F10 pela inviabilização de aproximadamente 100% das células em um período de 1,5h.Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2021-03-15T16:14:09Z No. of bitstreams: 2 Tese - Relton Romeis de Oliveira - 2020.pdf: 4855498 bytes, checksum: 0577b0ef6f1b0ebe1ce549c7b51190b7 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2021-03-17T12:07:03Z (GMT) No. of bitstreams: 2 Tese - Relton Romeis de Oliveira - 2020.pdf: 4855498 bytes, checksum: 0577b0ef6f1b0ebe1ce549c7b51190b7 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2021-03-17T12:07:03Z (GMT). No. of bitstreams: 2 Tese - Relton Romeis de Oliveira - 2020.pdf: 4855498 bytes, checksum: 0577b0ef6f1b0ebe1ce549c7b51190b7 (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2018-10-10Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciências da Saúde (FM)UFGBrasilFaculdade de Medicina - FM (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPaclitaxelMagnetossomasLiberação controlada e magnetohipertermiaPaclitaxelMagnetossomesControlled release and magnetohyperthermiaCIENCIAS DA SAUDEDesenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermiaDevelopment and evaluation of antitumoral activity in paclitaxel glass associated with magnetosomes by magnetohipertermiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis24500500500500191821reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALTese - Relton Romeis de Oliveira - 2020.pdfTese - Relton Romeis de Oliveira - 2020.pdfapplication/pdf4855498http://repositorio.bc.ufg.br/tede/bitstreams/5d47949c-ada4-4123-bbfb-ab98fcfb6706/download0577b0ef6f1b0ebe1ce549c7b51190b7MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/e500fd6f-ff06-474d-8015-ac230c762d8a/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/9d7c6885-796f-4c65-84db-cafad9e5d928/download4460e5956bc1d1639be9ae6146a50347MD52tede/111602021-03-17 09:07:04.523http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/11160http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2021-03-17T12:07:04Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
dc.title.alternative.eng.fl_str_mv |
Development and evaluation of antitumoral activity in paclitaxel glass associated with magnetosomes by magnetohipertermia |
title |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
spellingShingle |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia Oliveira, Relton Romeis de Paclitaxel Magnetossomas Liberação controlada e magnetohipertermia Paclitaxel Magnetossomes Controlled release and magnetohyperthermia CIENCIAS DA SAUDE |
title_short |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
title_full |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
title_fullStr |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
title_full_unstemmed |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
title_sort |
Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia |
author |
Oliveira, Relton Romeis de |
author_facet |
Oliveira, Relton Romeis de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lima, Eliana Martins |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7248774319455970 |
dc.contributor.referee1.fl_str_mv |
Lima, Eliana Martins |
dc.contributor.referee2.fl_str_mv |
Santos, Marcus Carrião |
dc.contributor.referee3.fl_str_mv |
Castro, Elisandra Gava de |
dc.contributor.referee4.fl_str_mv |
Rahal, Rosemar Macedo de Souza |
dc.contributor.referee5.fl_str_mv |
Nascimento, Thais Leite |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4031577881091710 |
dc.contributor.author.fl_str_mv |
Oliveira, Relton Romeis de |
contributor_str_mv |
Lima, Eliana Martins Lima, Eliana Martins Santos, Marcus Carrião Castro, Elisandra Gava de Rahal, Rosemar Macedo de Souza Nascimento, Thais Leite |
dc.subject.por.fl_str_mv |
Paclitaxel Magnetossomas Liberação controlada e magnetohipertermia |
topic |
Paclitaxel Magnetossomas Liberação controlada e magnetohipertermia Paclitaxel Magnetossomes Controlled release and magnetohyperthermia CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
Paclitaxel Magnetossomes Controlled release and magnetohyperthermia |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
This work describes the development and characterization of Magnetosomes Paclitaxel loaded (MS PTX-loaded) for cutaneous applications in magnetohyperthermia. Magnetic nanoparticles (MP) were prepared by coprecipitation of Fe (II) and Fe (III) or Mn (II) and Fe (III) salts in alkaline medium. MS were produced by hydration method of a preformed lipid film. The nanostructured system was characterized in terms of morphology, mean diameter and size distribution, encapsulation efficiency of PTX, stability and magnetic properties of magnetometry and magnetohyperthermia. MS PTX-loading had an average diameter of approximately 90nm with polydispersion index of 0.190. The PTX: lipid ratio was 1:50 (m/m) with 81% PTX encapsulation efficiency. Stability study of lyophilized MS PTX-loading showed 98% of the encapsulation efficiency for 60 days. MS PTX-loading presented superparamagnetic behavior in the presence of magnetic field, with saturation magnetization of 0.25 emu/g and volumetric fraction of 11.3x10-3. The concentration of MP in the formulation was 5.56mg/mL. The effect of magnetohyperthermia with variation of temperature of up to 30ºC was verified from the application of alternating magnetic field. This increase in MS temperature influenced the rate of in vitro release of the encapsulated PTX, allowing an increase of 400% release comparing temperatures of 25ºC and 43ºC. Thus, through the in vitro release study it was possible to perceive that magnetohyperthermia could act as a release trigger of PTX encapsulated in MS. From the biological assay of cytotoxicity with B16F10 cells it was possible to verify the cellular viability of the nanosystem and to determine the IC50. The application of a magnetic field together with the release of PTX showed a great potential for the cytotoxic action under the B16F10 strain by the impossibility of approximately 100% of the cells in a period of 1.5h. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-10-10 |
dc.date.accessioned.fl_str_mv |
2021-03-17T12:07:03Z |
dc.date.available.fl_str_mv |
2021-03-17T12:07:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, R. R. Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia. 2020. 106 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/11160 |
identifier_str_mv |
OLIVEIRA, R. R. Desenvolvimento e avaliação da atividade antitumoral in vitro de paclitaxel associado a magnetossomas por magnetohipertermia. 2020. 106 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/11160 |
dc.language.iso.fl_str_mv |
por |
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por |
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24 |
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500 500 500 500 |
dc.relation.department.fl_str_mv |
19 |
dc.relation.cnpq.fl_str_mv |
182 |
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1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Ciências da Saúde (FM) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Faculdade de Medicina - FM (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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