Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/10676 |
Resumo: | Vulvovaginal candidiasis is an infection caused by fungi of the genus Candida. The increased incidence of vulvovaginal candidiasis maybe related to the advancement of chronic and immunosuppressive diseases along with the emergence of new mechanisms of fungal resistance. The objective of the present work was to prepare and characterize chitosan nanoparticles containing fluconazole and trans-cinnamaldehyde co-encapsulated for the topical treatment of vulvovaginal candidiasis. The empty nanoparticles, fluconazole, trans-cinnamaldehyde and coencapsulated were prepared using the ionotropic gelling technique. The nanoparticles were characterized as to their average size and polydispersity index by the dynamic light scattering technique. The surface charge of the nanoparticles was obtained by the electrophoretic migration technique. The empty nanoparticles had an average size of 448,9 ± 32,9 nm, PdI of 0,4 ± 0,1 and a surface load of 31 ± 0,4 mV, the nanoparticles of fluconazole exhibited an average size of 175,3 ± 8,9 nm, PdI of 0,2 ± 0,01, surface load of 33,2 ± 0,3 mV and encapsulation efficiency of 37,5% ± 3,1. Trans-cinnamaldehyde nanoparticles showed an average size of 352,6 ± 71,6 nm, PdI of 0,4 ± 0,05, surface load of 44,6 ± 3,7 mV and encapsulation efficiency of 44,6 % ± 3,7 and the coencapsulated nanoparticles had an average size of 234,5 ± 60,2 nm, PdI of 0,4 ± 0,07, surface load of 33,2 ± 0,5 mV and encapsulation efficiency of 56,8% ± 3 for fluconazole and 46,1% ± 5,1 for trans-cinnamaldehyde. The scanning electron microscopy of the nanoparticles presented an oval shape. The nanoparticles had their antifungal efficacy against the strain of C. albicans ATCC 10231 investigated by means of the minimum inhibitory concentration by the broth microdilution technique. The fluconazole nanoparticle showed antifungal efficacy with a minimum inhibitory concentration of 2 μg/mL and the trans-cinnamaldehyde was effective in the concentration of 75 μg/mL. The coencapsulated nanoparticle exhibited a minimum inhibitory concentration of 2 μg/mL for fluconazole and 37,5 μg/mL for trans-cinnamaldehyde. The coencapsulated nanoparticle exhibited the same minimal inhibitory concentrations as the fluconazole and trans-cinnamaldehyde nanoparticles. As for in vitro toxicity, nanoparticles were considered non-toxic. Regarding the in vivo toxicity test, only the empty nanoparticles exhibited a cytotoxic effect. All groups of nanoparticles proved to be effective in inhibiting fungal growth. |
id |
UFG-2_eccc5d648f1295cc7de3a9e220e49dcd |
---|---|
oai_identifier_str |
oai:repositorio.bc.ufg.br:tede/10676 |
network_acronym_str |
UFG-2 |
network_name_str |
Repositório Institucional da UFG |
repository_id_str |
|
spelling |
Amaral, André Corrêahttp://lattes.cnpq.br/8801299423520104Amaral, André CorrêaAlves, Suzana FerreiraSilva, Luís Antônio Dantashttp://lattes.cnpq.br/0867077587097317Sousa, Paulo Henrique Dantas de2020-09-14T11:04:28Z2020-09-14T11:04:28Z2020-04-03SOUSA, P. H. D. Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal. 2020. 79 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/tede/handle/tede/10676Vulvovaginal candidiasis is an infection caused by fungi of the genus Candida. The increased incidence of vulvovaginal candidiasis maybe related to the advancement of chronic and immunosuppressive diseases along with the emergence of new mechanisms of fungal resistance. The objective of the present work was to prepare and characterize chitosan nanoparticles containing fluconazole and trans-cinnamaldehyde co-encapsulated for the topical treatment of vulvovaginal candidiasis. The empty nanoparticles, fluconazole, trans-cinnamaldehyde and coencapsulated were prepared using the ionotropic gelling technique. The nanoparticles were characterized as to their average size and polydispersity index by the dynamic light scattering technique. The surface charge of the nanoparticles was obtained by the electrophoretic migration technique. The empty nanoparticles had an average size of 448,9 ± 32,9 nm, PdI of 0,4 ± 0,1 and a surface load of 31 ± 0,4 mV, the nanoparticles of fluconazole exhibited an average size of 175,3 ± 8,9 nm, PdI of 0,2 ± 0,01, surface load of 33,2 ± 0,3 mV and encapsulation efficiency of 37,5% ± 3,1. Trans-cinnamaldehyde nanoparticles showed an average size of 352,6 ± 71,6 nm, PdI of 0,4 ± 0,05, surface load of 44,6 ± 3,7 mV and encapsulation efficiency of 44,6 % ± 3,7 and the coencapsulated nanoparticles had an average size of 234,5 ± 60,2 nm, PdI of 0,4 ± 0,07, surface load of 33,2 ± 0,5 mV and encapsulation efficiency of 56,8% ± 3 for fluconazole and 46,1% ± 5,1 for trans-cinnamaldehyde. The scanning electron microscopy of the nanoparticles presented an oval shape. The nanoparticles had their antifungal efficacy against the strain of C. albicans ATCC 10231 investigated by means of the minimum inhibitory concentration by the broth microdilution technique. The fluconazole nanoparticle showed antifungal efficacy with a minimum inhibitory concentration of 2 μg/mL and the trans-cinnamaldehyde was effective in the concentration of 75 μg/mL. The coencapsulated nanoparticle exhibited a minimum inhibitory concentration of 2 μg/mL for fluconazole and 37,5 μg/mL for trans-cinnamaldehyde. The coencapsulated nanoparticle exhibited the same minimal inhibitory concentrations as the fluconazole and trans-cinnamaldehyde nanoparticles. As for in vitro toxicity, nanoparticles were considered non-toxic. Regarding the in vivo toxicity test, only the empty nanoparticles exhibited a cytotoxic effect. All groups of nanoparticles proved to be effective in inhibiting fungal growth.A candidíase vulvovaginal é uma infecção causada por fungos do gênero Candida. O aumento da incidência de candidíase vulvovaginal pode estar relacionada com o avanço das doenças crônicas e imunossupressoras juntamente com o surgimento de novos mecanismos de resistência fúngica. O objetivo do presente trabalho foi preparar e caracterizar nanopartículas de quitosana contendo fluconazol e trans-cinamaldeído coencapsulados para o tratamento tópico da candidíase vulvovaginal. As nanopartículas vazias, de fluconazol, trans-cinamaldeído e coencapsuladas foram preparadas por meio da técnica de gelificação ionotrópica. As nanopartículas foram caracterizadas quanto ao tamanho médio e índice de polidispersão pela técnica de espalhamento dinâmico de luz. A carga superficial das nanopartículas foi obtida pela técnica de migração eletroforética. As nanopartículas vazias apresentaram tamanho médio de 448,9 ±32,9 nm, PdI de 0,4 ±0,1 e uma carga superficial de 31 ±0,4 mV, as nanopartículas de fluconazol exibiram um tamanho médio de 175,3 ±8,9 nm, PdI de 0,2 ±0,01, carga superficial de 33,2 ±0,3 mV e eficiência de encapsulação de 37,5 % ±3,1. Já as nanopartículas de trans-cinamaldeído demonstraram um tamanho médio de 352,6 ±71,6 nm, PdI de 0,4 ±0,05, carga superficial de 44,6 ±3,7 mV e eficiência de encapsulação de 44,6% ±3,7 e as nanopartículas coencapsuladas apresentaram um tamanho médio de 234,5 ±60,2 nm, PdI de 0,4 ±0,07, carga superficial de 33,2 ±0,5 mV e eficiência de encapsulação de 56,8% ±3 para o fluconazol e 46,1% ±5,1 para o trans-cinamaldeído. A microscopia eletrônica de varredura as nanopartículas apresentaram um formato oval. As nanopartículas tiveram sua eficácia antifúngica frente a cepa de C. albicans ATCC 10231 investigada por meio da concentração inibitória mínima pela técnica de microdiluição em caldo. A nanopartícula de fluconazol apresentou eficácia antifúngica com concentração inibitória mínima de 2 μg/mL e a de trans-cinamaldeído foi eficaz na concentração de 75 μg/mL. A nanopartícula coencapsulada exibiu uma concentração inibitória mínima de 2 μg/mL para o fluconazol e 37,5 μg/mL para o trans-cinamaldeído. Quanto a toxicidade in vitro as nanopartículas foram consideradas atóxicas. Em relação ao teste in vivo de toxicidade, apenas as nanopartículas vazias exibiram um efeito citotóxico. Todos os grupos de nanopartículas mostraram-se eficazes no processo de inibição do crescimento fúngico.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2020-09-13T18:34:20Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Paulo Henrique Dantas de Sousa - 2020.pdf: 3787003 bytes, checksum: cf51136bc130a68e0427f7e77588aaa4 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-09-14T11:04:27Z (GMT) No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Paulo Henrique Dantas de Sousa - 2020.pdf: 3787003 bytes, checksum: cf51136bc130a68e0427f7e77588aaa4 (MD5)Made available in DSpace on 2020-09-14T11:04:28Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação - Paulo Henrique Dantas de Sousa - 2020.pdf: 3787003 bytes, checksum: cf51136bc130a68e0427f7e77588aaa4 (MD5) Previous issue date: 2020-04-03OutroporUniversidade Federal de GoiásPrograma de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCandidíase vulvovaginalGelificação ionotrópicaNanopartículas poliméricasCandidíase vulvovaginal murinaResistência fúngicaFungal resistanceKey wordsVulvovaginal candidiasisIonotropic gelationPolymeric nanoparticlesMurine vulvovaginal candidiasisCIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIACo-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginalCo-encapsulation of trans-cinnamaldehyde and fluconazole in chitosan nanoparticles for topical treatment of vulvovaginal candidiasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis15500500500500291115reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/599d257d-45c6-457e-9f97-556cf458145a/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811http://repositorio.bc.ufg.br/tede/bitstreams/0cb02a54-e3d7-4663-9567-b2718a13b2dc/downloade39d27027a6cc9cb039ad269a5db8e34MD52ORIGINALDissertação - Paulo Henrique Dantas de Sousa - 2020.pdfDissertação - Paulo Henrique Dantas de Sousa - 2020.pdfapplication/pdf3787003http://repositorio.bc.ufg.br/tede/bitstreams/d16c0fcc-1a04-4c67-869b-8e970e166f06/downloadcf51136bc130a68e0427f7e77588aaa4MD53tede/106762020-09-14 08:04:28.281http://creativecommons.org/licenses/by-nc-nd/3.0/br/Attribution-NonCommercial-NoDerivs 3.0 Brazilopen.accessoai:repositorio.bc.ufg.br:tede/10676http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2020-09-14T11:04:28Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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 |
dc.title.pt_BR.fl_str_mv |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
dc.title.alternative.eng.fl_str_mv |
Co-encapsulation of trans-cinnamaldehyde and fluconazole in chitosan nanoparticles for topical treatment of vulvovaginal candidiasis |
title |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
spellingShingle |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal Sousa, Paulo Henrique Dantas de Candidíase vulvovaginal Gelificação ionotrópica Nanopartículas poliméricas Candidíase vulvovaginal murina Resistência fúngica Fungal resistance Key words Vulvovaginal candidiasis Ionotropic gelation Polymeric nanoparticles Murine vulvovaginal candidiasis CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
title_short |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
title_full |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
title_fullStr |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
title_full_unstemmed |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
title_sort |
Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal |
author |
Sousa, Paulo Henrique Dantas de |
author_facet |
Sousa, Paulo Henrique Dantas de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Amaral, André Corrêa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8801299423520104 |
dc.contributor.referee1.fl_str_mv |
Amaral, André Corrêa |
dc.contributor.referee2.fl_str_mv |
Alves, Suzana Ferreira |
dc.contributor.referee3.fl_str_mv |
Silva, Luís Antônio Dantas |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0867077587097317 |
dc.contributor.author.fl_str_mv |
Sousa, Paulo Henrique Dantas de |
contributor_str_mv |
Amaral, André Corrêa Amaral, André Corrêa Alves, Suzana Ferreira Silva, Luís Antônio Dantas |
dc.subject.por.fl_str_mv |
Candidíase vulvovaginal Gelificação ionotrópica Nanopartículas poliméricas Candidíase vulvovaginal murina Resistência fúngica Fungal resistance |
topic |
Candidíase vulvovaginal Gelificação ionotrópica Nanopartículas poliméricas Candidíase vulvovaginal murina Resistência fúngica Fungal resistance Key words Vulvovaginal candidiasis Ionotropic gelation Polymeric nanoparticles Murine vulvovaginal candidiasis CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
dc.subject.eng.fl_str_mv |
Key words Vulvovaginal candidiasis Ionotropic gelation Polymeric nanoparticles Murine vulvovaginal candidiasis |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA |
description |
Vulvovaginal candidiasis is an infection caused by fungi of the genus Candida. The increased incidence of vulvovaginal candidiasis maybe related to the advancement of chronic and immunosuppressive diseases along with the emergence of new mechanisms of fungal resistance. The objective of the present work was to prepare and characterize chitosan nanoparticles containing fluconazole and trans-cinnamaldehyde co-encapsulated for the topical treatment of vulvovaginal candidiasis. The empty nanoparticles, fluconazole, trans-cinnamaldehyde and coencapsulated were prepared using the ionotropic gelling technique. The nanoparticles were characterized as to their average size and polydispersity index by the dynamic light scattering technique. The surface charge of the nanoparticles was obtained by the electrophoretic migration technique. The empty nanoparticles had an average size of 448,9 ± 32,9 nm, PdI of 0,4 ± 0,1 and a surface load of 31 ± 0,4 mV, the nanoparticles of fluconazole exhibited an average size of 175,3 ± 8,9 nm, PdI of 0,2 ± 0,01, surface load of 33,2 ± 0,3 mV and encapsulation efficiency of 37,5% ± 3,1. Trans-cinnamaldehyde nanoparticles showed an average size of 352,6 ± 71,6 nm, PdI of 0,4 ± 0,05, surface load of 44,6 ± 3,7 mV and encapsulation efficiency of 44,6 % ± 3,7 and the coencapsulated nanoparticles had an average size of 234,5 ± 60,2 nm, PdI of 0,4 ± 0,07, surface load of 33,2 ± 0,5 mV and encapsulation efficiency of 56,8% ± 3 for fluconazole and 46,1% ± 5,1 for trans-cinnamaldehyde. The scanning electron microscopy of the nanoparticles presented an oval shape. The nanoparticles had their antifungal efficacy against the strain of C. albicans ATCC 10231 investigated by means of the minimum inhibitory concentration by the broth microdilution technique. The fluconazole nanoparticle showed antifungal efficacy with a minimum inhibitory concentration of 2 μg/mL and the trans-cinnamaldehyde was effective in the concentration of 75 μg/mL. The coencapsulated nanoparticle exhibited a minimum inhibitory concentration of 2 μg/mL for fluconazole and 37,5 μg/mL for trans-cinnamaldehyde. The coencapsulated nanoparticle exhibited the same minimal inhibitory concentrations as the fluconazole and trans-cinnamaldehyde nanoparticles. As for in vitro toxicity, nanoparticles were considered non-toxic. Regarding the in vivo toxicity test, only the empty nanoparticles exhibited a cytotoxic effect. All groups of nanoparticles proved to be effective in inhibiting fungal growth. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-09-14T11:04:28Z |
dc.date.available.fl_str_mv |
2020-09-14T11:04:28Z |
dc.date.issued.fl_str_mv |
2020-04-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUSA, P. H. D. Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal. 2020. 79 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2020. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10676 |
identifier_str_mv |
SOUSA, P. H. D. Co-encapsulação do trans-cinamaldeído e do fluconazol em nanopartículas de quitosana para o tratamento tópico da candidíase vulvovaginal. 2020. 79 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2020. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10676 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
15 |
dc.relation.confidence.fl_str_mv |
500 500 500 500 |
dc.relation.department.fl_str_mv |
29 |
dc.relation.cnpq.fl_str_mv |
111 |
dc.relation.sponsorship.fl_str_mv |
5 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/599d257d-45c6-457e-9f97-556cf458145a/download http://repositorio.bc.ufg.br/tede/bitstreams/0cb02a54-e3d7-4663-9567-b2718a13b2dc/download http://repositorio.bc.ufg.br/tede/bitstreams/d16c0fcc-1a04-4c67-869b-8e970e166f06/download |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 e39d27027a6cc9cb039ad269a5db8e34 cf51136bc130a68e0427f7e77588aaa4 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1798044301896187904 |