Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/10471 |
Resumo: | Taenia crassiceps cysticercosis is a rare disease in humans and domestic animals, however there are reports of cases of humans as paratenic hosts, especially immunocompromised ones which indicates this parasite as of zoonotic risk. Albendazole and praziquantel are widely used drugs in cysticercosis treatment as they are well tolerated by patients. Biochemically, the active forms of albendazole inhibit the glucose uptake which leads to energy production breakdown and death of the parasite. The active forms of praziquantel induce muscle contractions, tegument damages and metabolic alterations. Throughout the life cycle, the energetic metabolism of T. crassiceps varies accordingly to nutrient sources and oxygen tension. Cysticerci use glucose and glycogen as energy source and reserve substances, respectively, and the products generated from their degradation are used in other metabolic pathways. Besides, the parasite is capable of fatty acid oxidation and amino acids breakdown as alternative energy production sources. The aim of this study was to evaluate the biochemical alterations, in vivo, in T. crassiceps cysticerci after treatment with low doses of albendazole and praziquantel. The cysticerci were inoculated into the peritoneal cavity of female BALB/c mice which after 30 days of infection received treatment with one of the following dosages: 5,75 or 11,5 mg/kg of albendazole or 3,83 or 7,67 mg/kg of praziquantel. After 24h the animals were euthanized and the cysticerci removed and classified as belonging to initial, larval or final stage accordingly to their morphologic characteristics. The cysticerci were analyzed through high performance liquid chromatography aiming the detection of organic acids from glycolisis, tricarboxylic acid cycle and fatty acid oxidation, and through spectrophotometry aiming the quantification of glucose, urea and creatinine. The low dosage treatment induced a partial blockage of the glucose uptake by the cysticerci. The concentrations of the drugs used were capable of activation of the tricarboxylic acid cycle which are considered energetically more profitable which was reflected by the increase in the concentrations of citrate, malate and α-cetoglutarato, which was reported in cysticerci for the first time. The used dosages of the drugs induced alterations in the parasite’s metabolism increasing the use of alternative energy production pathways such as the fatty acid oxidation, detected by the presence of propionate and β-hydroxibutyrate, and the aminoacids breakdown, detected by the urea production. |
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Vinaud, Marina Clarehttp://lattes.cnpq.br/1921551651088660Lino Junior, Ruy de Souzahttp://lattes.cnpq.br/0372118837748010Vinaud, Marina ClareOliveira, Valéria deBezerra, José Clecildo Barretohttp://lattes.cnpq.br/4916519165378614Fraga, Carolina Miguel2020-03-27T13:21:24Z2011-02-21FRAGA, C. M. Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps. 2011. 67 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2011.http://repositorio.bc.ufg.br/tede/handle/tede/10471ark:/38995/0013000005661Taenia crassiceps cysticercosis is a rare disease in humans and domestic animals, however there are reports of cases of humans as paratenic hosts, especially immunocompromised ones which indicates this parasite as of zoonotic risk. Albendazole and praziquantel are widely used drugs in cysticercosis treatment as they are well tolerated by patients. Biochemically, the active forms of albendazole inhibit the glucose uptake which leads to energy production breakdown and death of the parasite. The active forms of praziquantel induce muscle contractions, tegument damages and metabolic alterations. Throughout the life cycle, the energetic metabolism of T. crassiceps varies accordingly to nutrient sources and oxygen tension. Cysticerci use glucose and glycogen as energy source and reserve substances, respectively, and the products generated from their degradation are used in other metabolic pathways. Besides, the parasite is capable of fatty acid oxidation and amino acids breakdown as alternative energy production sources. The aim of this study was to evaluate the biochemical alterations, in vivo, in T. crassiceps cysticerci after treatment with low doses of albendazole and praziquantel. The cysticerci were inoculated into the peritoneal cavity of female BALB/c mice which after 30 days of infection received treatment with one of the following dosages: 5,75 or 11,5 mg/kg of albendazole or 3,83 or 7,67 mg/kg of praziquantel. After 24h the animals were euthanized and the cysticerci removed and classified as belonging to initial, larval or final stage accordingly to their morphologic characteristics. The cysticerci were analyzed through high performance liquid chromatography aiming the detection of organic acids from glycolisis, tricarboxylic acid cycle and fatty acid oxidation, and through spectrophotometry aiming the quantification of glucose, urea and creatinine. The low dosage treatment induced a partial blockage of the glucose uptake by the cysticerci. The concentrations of the drugs used were capable of activation of the tricarboxylic acid cycle which are considered energetically more profitable which was reflected by the increase in the concentrations of citrate, malate and α-cetoglutarato, which was reported in cysticerci for the first time. The used dosages of the drugs induced alterations in the parasite’s metabolism increasing the use of alternative energy production pathways such as the fatty acid oxidation, detected by the presence of propionate and β-hydroxibutyrate, and the aminoacids breakdown, detected by the urea production.A cisticercose por Taenia crassiceps é uma doença rara em humanos e em animais domésticos, entretanto existem relatos de casos indicando que o homem pode atuar como hospedeiro paratênico, atingindo principalmente pacientes imunocomprometidos, constituindo-se assim um risco zoonótico. O albendazol e o praziquantel são fármacos amplamente utilizados no tratamento de cisticercose, por serem muito bem tolerados pelo organismo. Bioquimicamente, as formas ativas do albendazol inibem a captação de glicose, tornando o nível de energia inadequado para a sobrevivência do helminto. Já as formas ativas do praziquantel provocam contrações musculares, danos no tegumento e consequentes alterações metabólicas. Ao longo de seu ciclo de vida, o metabolismo energético da T. crassiceps varia de acordo com o predomínio das fontes de nutrientes e tensão de oxigênio. Os cisticercos utilizam a glicose e o glicogênio como fonte e reserva energética. Os produtos gerados a partir da degradação dessas substâncias são utilizados em vias do metabolismo. Além disso, o parasito também é capaz de oxidar ácidos graxos e de utilizar aminoácidos como fontes alternativas de produção de energia. O objetivo deste trabalho foi o de avaliar as alterações bioquímicas, in vivo, de cisticercos de T. crassiceps após o tratamento com baixas doses de albendazol e praziquantel. Os cisticercos foram inoculados na cavidade peritoneal de camundongos BALB/c fêmeas que, após 30 dias de infecção, foram tratados por gavagem com 5,75 e 11,5 mg/kg de albendazol e 3,83 e 7,67 mg/kg de praziquantel. Após 24h, os animais foram eutanasiados e os cisticercos retirados foram classificados, segundo seu estádio evolutivo, em inicial, larval e final, de acordo com características morfológicas. Em seguida, fez-se a análise cromatográfica para detecção de ácidos orgânicos que indicam glicólise, ciclo do ácido cítrico e oxidação de ácidos graxos; e análise espectrofotométrica para quantificação de glicose, uréia e creatinina, nas diferentes formas evolutivas do parasito. O tratamento com baixas doses dos fármacos causou um bloqueio parcial da captação de glicose pelos cisticercos. As concentrações dos fármacos utilizadas foram capazes de ativar vias do ciclo do ácido cítrico consideradas mais rentáveis energeticamente, diante do aumento nas concentrações detectadas de citrato, malato, α-cetoglutarato, sendo este último relatado pela primeira vez. As dosagens utilizadas foram capazes de alterar o metabolismo do parasito, induzindo a utilização de vias alternativas de produção de energia, como a oxidação de ácidos graxos, pela detecção de propionato e β-hidroxibutirato, e a utilização de aminoácidos, pela detecção de uréia.Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2020-03-27T12:17:29Z No. of bitstreams: 2 Dissertação - Carolina Miguel Fraga - 2011.pdf: 4974413 bytes, checksum: 4e284710a62110d7ae957a18b0f4afeb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2020-03-27T13:21:24Z (GMT) No. of bitstreams: 2 Dissertação - Carolina Miguel Fraga - 2011.pdf: 4974413 bytes, checksum: 4e284710a62110d7ae957a18b0f4afeb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2020-03-27T13:21:24Z (GMT). No. of bitstreams: 2 Dissertação - Carolina Miguel Fraga - 2011.pdf: 4974413 bytes, checksum: 4e284710a62110d7ae957a18b0f4afeb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2011-02-21Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTaenia crassicepsTratamento in vivoMetabolismo energéticoMetabolismo respiratórioÁcidos orgânicosIn vivo treatmentEnergetic metabolismRespiratory metabolismOrganic acidsCIENCIAS BIOLOGICAS::PARASITOLOGIAAvaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassicepsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6085308344741430434600600600600-7769011444564556288-4544576747271574306-2555911436985713659reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Carolina Miguel Fraga - 2011.pdfDissertação - Carolina Miguel Fraga - 2011.pdfapplication/pdf4974413http://repositorio.bc.ufg.br/tede/bitstreams/e4c14334-b736-4298-828f-d7daa4899a7d/download4e284710a62110d7ae957a18b0f4afebMD55LICENSElicense.txtlicense.txttext/plain; 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dc.title.eng.fl_str_mv |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
title |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
spellingShingle |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps Fraga, Carolina Miguel Taenia crassiceps Tratamento in vivo Metabolismo energético Metabolismo respiratório Ácidos orgânicos In vivo treatment Energetic metabolism Respiratory metabolism Organic acids CIENCIAS BIOLOGICAS::PARASITOLOGIA |
title_short |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
title_full |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
title_fullStr |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
title_full_unstemmed |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
title_sort |
Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps |
author |
Fraga, Carolina Miguel |
author_facet |
Fraga, Carolina Miguel |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vinaud, Marina Clare |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1921551651088660 |
dc.contributor.advisor-co1.fl_str_mv |
Lino Junior, Ruy de Souza |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0372118837748010 |
dc.contributor.referee1.fl_str_mv |
Vinaud, Marina Clare |
dc.contributor.referee2.fl_str_mv |
Oliveira, Valéria de |
dc.contributor.referee3.fl_str_mv |
Bezerra, José Clecildo Barreto |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4916519165378614 |
dc.contributor.author.fl_str_mv |
Fraga, Carolina Miguel |
contributor_str_mv |
Vinaud, Marina Clare Lino Junior, Ruy de Souza Vinaud, Marina Clare Oliveira, Valéria de Bezerra, José Clecildo Barreto |
dc.subject.por.fl_str_mv |
Taenia crassiceps Tratamento in vivo Metabolismo energético Metabolismo respiratório Ácidos orgânicos |
topic |
Taenia crassiceps Tratamento in vivo Metabolismo energético Metabolismo respiratório Ácidos orgânicos In vivo treatment Energetic metabolism Respiratory metabolism Organic acids CIENCIAS BIOLOGICAS::PARASITOLOGIA |
dc.subject.eng.fl_str_mv |
In vivo treatment Energetic metabolism Respiratory metabolism Organic acids |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::PARASITOLOGIA |
description |
Taenia crassiceps cysticercosis is a rare disease in humans and domestic animals, however there are reports of cases of humans as paratenic hosts, especially immunocompromised ones which indicates this parasite as of zoonotic risk. Albendazole and praziquantel are widely used drugs in cysticercosis treatment as they are well tolerated by patients. Biochemically, the active forms of albendazole inhibit the glucose uptake which leads to energy production breakdown and death of the parasite. The active forms of praziquantel induce muscle contractions, tegument damages and metabolic alterations. Throughout the life cycle, the energetic metabolism of T. crassiceps varies accordingly to nutrient sources and oxygen tension. Cysticerci use glucose and glycogen as energy source and reserve substances, respectively, and the products generated from their degradation are used in other metabolic pathways. Besides, the parasite is capable of fatty acid oxidation and amino acids breakdown as alternative energy production sources. The aim of this study was to evaluate the biochemical alterations, in vivo, in T. crassiceps cysticerci after treatment with low doses of albendazole and praziquantel. The cysticerci were inoculated into the peritoneal cavity of female BALB/c mice which after 30 days of infection received treatment with one of the following dosages: 5,75 or 11,5 mg/kg of albendazole or 3,83 or 7,67 mg/kg of praziquantel. After 24h the animals were euthanized and the cysticerci removed and classified as belonging to initial, larval or final stage accordingly to their morphologic characteristics. The cysticerci were analyzed through high performance liquid chromatography aiming the detection of organic acids from glycolisis, tricarboxylic acid cycle and fatty acid oxidation, and through spectrophotometry aiming the quantification of glucose, urea and creatinine. The low dosage treatment induced a partial blockage of the glucose uptake by the cysticerci. The concentrations of the drugs used were capable of activation of the tricarboxylic acid cycle which are considered energetically more profitable which was reflected by the increase in the concentrations of citrate, malate and α-cetoglutarato, which was reported in cysticerci for the first time. The used dosages of the drugs induced alterations in the parasite’s metabolism increasing the use of alternative energy production pathways such as the fatty acid oxidation, detected by the presence of propionate and β-hydroxibutyrate, and the aminoacids breakdown, detected by the urea production. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-21 |
dc.date.accessioned.fl_str_mv |
2020-03-27T13:21:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FRAGA, C. M. Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps. 2011. 67 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/10471 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000005661 |
identifier_str_mv |
FRAGA, C. M. Avaliação bioquímica in vivo do tratamento anti-helmíntico de cisticercos de Taenia crassiceps. 2011. 67 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2011. ark:/38995/0013000005661 |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/10471 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6085308344741430434 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-7769011444564556288 |
dc.relation.cnpq.fl_str_mv |
-4544576747271574306 |
dc.relation.sponsorship.fl_str_mv |
-2555911436985713659 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
reponame_str |
Repositório Institucional da UFG |
collection |
Repositório Institucional da UFG |
bitstream.url.fl_str_mv |
http://repositorio.bc.ufg.br/tede/bitstreams/e4c14334-b736-4298-828f-d7daa4899a7d/download http://repositorio.bc.ufg.br/tede/bitstreams/aca6a2b6-0bf8-4d3d-8494-5c703c793ae2/download http://repositorio.bc.ufg.br/tede/bitstreams/94c2d965-8fa6-49fd-850e-9af4f80b3d6d/download http://repositorio.bc.ufg.br/tede/bitstreams/d105589d-7691-4343-8354-acdd891cabc5/download http://repositorio.bc.ufg.br/tede/bitstreams/f111c16d-95fb-4a58-8580-addf76e2db6a/download |
bitstream.checksum.fl_str_mv |
4e284710a62110d7ae957a18b0f4afeb bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1811721396440530944 |