Neurocisticercose experimental: avaliação da atividade da nitazoxanida no metabolismo de cisticercos de Taenia crassiceps

• Autor(a) principal: Lima, Nayana Ferreira de
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/001300000dmwb
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9900
• topic: Piruvato ferrodoxina oxidoredutase; Metabolismo; Neurocisticercose; Nitazoxanida; Taenia crassiceps; Pyruvate ferredoxin oxidoreductase enzyme; Metabolism, experimental neurocysticercosis; Nitazoxanide; CIENCIAS BIOLOGICAS::PARASITOLOGIA

Neurocysticercosis (NCC) is the most frequente helminthiasis that aflicts the central nervous system and is caused by the presence of Taenia solium cysticerci. Experimental models are usefull tools to understand the host-parasite interaction in human cysticercosis studies. The most used parasite for such studies is T. crassiceps cysticercus due to its quick developing cycle, easy maintenance and, mainly, due to its antigenic similarity to T. solium. Nitazoxanide (NTZ) is an antiparasitary drug from the nitrotiazol group with ample spectre of activity against protozoans, bacteria, nematodes and trematodes. NTZ presents as mode of action the blockage of pyruvate ferredoxin oxidoreductase enzyme (PFOR). The aim of this study was to determine the effect of two dosages of NTZ on the energetic metabolism of T. crassiceps cysticerci (ORF strain) inoculated in BALB/c female mice brain. The animals were divided into three groups that received one oral dose of physiological solution (NaCl 0.9%) – Group 1; 20 mg/Kg of NTZ – Group 2; 40 mg/Kg of NTZ – Group 3. All animals were treated after 30 days of infection and were euthanized 24h after treatment. The experiment was performed in 6 independent repetitions. The metabolic pathways of glycolysis, citric acid cycle, fatty acids oxidation and proteins catabolism were quantified through high performance liquid chromatography (HPLC) and spectrophotometry. The organic acids detected in the treated samples indicated the glycolytic pathway and lactic fermentation, fatty acids oxidation and proteins catabolism in order to produce energy. Acetate and acetoacetate were not detected in the NTZ treated groups as a consequence of the drug’s mode of action. In conclusion the NTZ treated cysticerci used the fumarate reductase, fatty acids oxidation and proteins catabolism as energy production pathways probably due to the impairment of the PFOR enzyme activity.