Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/6638 |
Resumo: | Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. The fungus grows as mycelium in the saprophytic environment, with the infection caused mycelium propagules and/or conidia, that will differentiate into the yeast form. Copper is an essential micronutrient for eukaryotes and bacteria, is extremely important to various proteins, and cofactor of many enzymes and used in many biochemical processes. However, excessive, copper can cause damage to proteins, lipids and nucleic acids, inferring the importance of this metal homeostasis. The objective of this study was to evaluate the fungus Paracoccidioides sp behavior in the presence of copper excess, through proteomics analysis. In the presence of copper excess, the yeast colonies of Paracoccidioides sp. depicted brown color. Expression of transcripts encoding CTR3, a copper transporter and the copper chaperone ATX1 showed inhibition and induction, respectively in the presence of excess copper, as verified by qRT-PCR. Proteins related to stress responses, with melanin production and pathways for energy, are regulated, suggesting that Paracoccidioides sp. responds to copper excess with detoxification strategies, protection and induction of various pathways of obtaining energy. |
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Soares, Célia Maria de Almeidahttp://lattes.cnpq.br/8539946335852637Lima, Patrícia de Sousahttp://lattes.cnpq.br/8330747475582900Soares, Célia Maria de AlmeidaBorges, Clayton LuizBaeza, Lilian CristianeTauhata, SinjiBrito, Wesley de Almeidahttp://lattes.cnpq.br/7779174594279592Portis, Igor Godinho2016-12-26T14:18:16Z2015-02-24PORTIS, I. G. Análise proteômica em células leveduriformes do fungo paracoccidioides sp. em excesso de cobre. 2015. 65 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2015.http://repositorio.bc.ufg.br/tede/handle/tede/6638Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. The fungus grows as mycelium in the saprophytic environment, with the infection caused mycelium propagules and/or conidia, that will differentiate into the yeast form. Copper is an essential micronutrient for eukaryotes and bacteria, is extremely important to various proteins, and cofactor of many enzymes and used in many biochemical processes. However, excessive, copper can cause damage to proteins, lipids and nucleic acids, inferring the importance of this metal homeostasis. The objective of this study was to evaluate the fungus Paracoccidioides sp behavior in the presence of copper excess, through proteomics analysis. In the presence of copper excess, the yeast colonies of Paracoccidioides sp. depicted brown color. Expression of transcripts encoding CTR3, a copper transporter and the copper chaperone ATX1 showed inhibition and induction, respectively in the presence of excess copper, as verified by qRT-PCR. Proteins related to stress responses, with melanin production and pathways for energy, are regulated, suggesting that Paracoccidioides sp. responds to copper excess with detoxification strategies, protection and induction of various pathways of obtaining energy.Paracoccidioidomicose é a micose sistêmica mais prevalente da América Latina, causada por fungos do gênero Paracoccidioides spp. O fungo cresce como micélio no ambiente saprofítico, sendo a infecção causada por propágulos de micélio e ou conídios, que se diferenciam na forma leveduriforme no hospedeiro. O cobre é um micronutriente essencial para eucariotos e bactérias, de extrema importância a várias proteínas, sendo cofator de muitas enzimas e utilizado em diversos processos bioquímicos. Entretanto em excesso, o cobre pode provocar danos a proteínas, lipídeos e ácidos nucléicos, por isso a importância da homeostase desse metal. O objetivo deste estudo foi avaliar o comportamento do fungo Paracoccidioides sp. em presença de excesso de cobre, através de análise proteômica. Na presença de excesso de cobre, as colônias leveduriformes de Paracoccidioides sp. apresentaram coloração marrom. A expressão dos transcritos codificantes do transportador de cobre Ctr3 e da chaperona Atx1, apresentaram inibição e indução, respectivamente na presença de excesso de cobre, como verificado através de qRT-PCR. Proteínas relacionadas a respostas ao estresse, com produção de melanina e com vias para obtenção de energia, foram reguladas, o que sugere que Paracoccidioides sp. responde ao estresse de excesso de cobre com estratégias de desintoxicação celular, proteção e indução de várias vias de obtenção de energia.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-12-21T15:53:06Z No. of bitstreams: 2 Dissertação - Igor Godinho Portis - 2015.pdf: 1381751 bytes, checksum: 82c1841407115b20ec4e6f5d2f786b17 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-12-26T14:18:16Z (GMT) No. of bitstreams: 2 Dissertação - Igor Godinho Portis - 2015.pdf: 1381751 bytes, checksum: 82c1841407115b20ec4e6f5d2f786b17 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2016-12-26T14:18:16Z (GMT). No. of bitstreams: 2 Dissertação - Igor Godinho Portis - 2015.pdf: 1381751 bytes, checksum: 82c1841407115b20ec4e6f5d2f786b17 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-02-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Genética e Biologia MolecularUFGBrasilInstituto de Ciências Biológicas - ICB (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFungoMetabolismoMelaninaVirulênciaBiologia molecularFungiMetabolismoMelaninVirulenceMolecular biologyCIENCIAS BIOLOGICAS::GENETICAAnálise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobreProteomic analysis in yeast cells Paracoccidioides sp. on copper overloadinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7015780075895009588600600600600-3872772117827373404-55181442685852520512075167498588264571reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
dc.title.alternative.eng.fl_str_mv |
Proteomic analysis in yeast cells Paracoccidioides sp. on copper overload |
title |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
spellingShingle |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre Portis, Igor Godinho Fungo Metabolismo Melanina Virulência Biologia molecular Fungi Metabolismo Melanin Virulence Molecular biology CIENCIAS BIOLOGICAS::GENETICA |
title_short |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
title_full |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
title_fullStr |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
title_full_unstemmed |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
title_sort |
Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre |
author |
Portis, Igor Godinho |
author_facet |
Portis, Igor Godinho |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Soares, Célia Maria de Almeida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8539946335852637 |
dc.contributor.advisor-co1.fl_str_mv |
Lima, Patrícia de Sousa |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8330747475582900 |
dc.contributor.referee1.fl_str_mv |
Soares, Célia Maria de Almeida |
dc.contributor.referee2.fl_str_mv |
Borges, Clayton Luiz |
dc.contributor.referee3.fl_str_mv |
Baeza, Lilian Cristiane |
dc.contributor.referee4.fl_str_mv |
Tauhata, Sinji |
dc.contributor.referee5.fl_str_mv |
Brito, Wesley de Almeida |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7779174594279592 |
dc.contributor.author.fl_str_mv |
Portis, Igor Godinho |
contributor_str_mv |
Soares, Célia Maria de Almeida Lima, Patrícia de Sousa Soares, Célia Maria de Almeida Borges, Clayton Luiz Baeza, Lilian Cristiane Tauhata, Sinji Brito, Wesley de Almeida |
dc.subject.por.fl_str_mv |
Fungo Metabolismo Melanina Virulência Biologia molecular |
topic |
Fungo Metabolismo Melanina Virulência Biologia molecular Fungi Metabolismo Melanin Virulence Molecular biology CIENCIAS BIOLOGICAS::GENETICA |
dc.subject.eng.fl_str_mv |
Fungi Metabolismo Melanin Virulence Molecular biology |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::GENETICA |
description |
Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. The fungus grows as mycelium in the saprophytic environment, with the infection caused mycelium propagules and/or conidia, that will differentiate into the yeast form. Copper is an essential micronutrient for eukaryotes and bacteria, is extremely important to various proteins, and cofactor of many enzymes and used in many biochemical processes. However, excessive, copper can cause damage to proteins, lipids and nucleic acids, inferring the importance of this metal homeostasis. The objective of this study was to evaluate the fungus Paracoccidioides sp behavior in the presence of copper excess, through proteomics analysis. In the presence of copper excess, the yeast colonies of Paracoccidioides sp. depicted brown color. Expression of transcripts encoding CTR3, a copper transporter and the copper chaperone ATX1 showed inhibition and induction, respectively in the presence of excess copper, as verified by qRT-PCR. Proteins related to stress responses, with melanin production and pathways for energy, are regulated, suggesting that Paracoccidioides sp. responds to copper excess with detoxification strategies, protection and induction of various pathways of obtaining energy. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-24 |
dc.date.accessioned.fl_str_mv |
2016-12-26T14:18:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTIS, I. G. Análise proteômica em células leveduriformes do fungo paracoccidioides sp. em excesso de cobre. 2015. 65 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/6638 |
identifier_str_mv |
PORTIS, I. G. Análise proteômica em células leveduriformes do fungo paracoccidioides sp. em excesso de cobre. 2015. 65 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2015. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/6638 |
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por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidade Federal de Goiás |
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Brasil |
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Universidade Federal de Goiás |
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