EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS

Detalhes bibliográficos
Autor(a) principal: Ramos, Cristiane Regina
Data de Publicação: 2008
Outros Autores: Brasil, Edward Madureira, Geraldine, Robson Maia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/4158
Resumo: A comparative evaluation process was carried out between two methods of aflatoxins extraction, cleaning, and purification, for High Performance Liquid Chromatography (HPLC) analysis. The first one is the physical-chemical method, based on chemical reactions for all the stages, and the second makes use of a immunoaffinity column (IAC). The methods were evaluated as related to recovery percentage, cost, time spent for analysis, and number of samples analyzed per day. The recovery percentage obtained through the physical-chemical method was lower than the IAC method to B1, B2 and G1 aflatoxins, and higher to G2 aflatoxin. Both techniques presented recovery percentages in accordance with established values by the European Community (70% to 120%). Concerning costs, the physical-chemical method showed to be the least expensive, but its analysis process takes more time, because it involves more steps and demands more caution from laboratory technicians. Despite its higher cost, about six times higher than the physicalchemical method, the IAC method showed to be faster and easier to perform. KEY-WORDS: Recovery; aflatoxins; extraction methods; immunoaffinity; HPLC.
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spelling EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSISAVALIAÇÃO DE MÉTODOS DE EXTRAÇÃO, LIMPEZA E PURIFICAÇÃO DE AFLATOXINAS PARA ANÁLISE EM CROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIARecoveryaflatoxinsextraction methodsimmunoaffinityHPLCRecuperaçãoaflatoxinasmétodos de extraçãoimunoafinidadeCLAE A comparative evaluation process was carried out between two methods of aflatoxins extraction, cleaning, and purification, for High Performance Liquid Chromatography (HPLC) analysis. The first one is the physical-chemical method, based on chemical reactions for all the stages, and the second makes use of a immunoaffinity column (IAC). The methods were evaluated as related to recovery percentage, cost, time spent for analysis, and number of samples analyzed per day. The recovery percentage obtained through the physical-chemical method was lower than the IAC method to B1, B2 and G1 aflatoxins, and higher to G2 aflatoxin. Both techniques presented recovery percentages in accordance with established values by the European Community (70% to 120%). Concerning costs, the physical-chemical method showed to be the least expensive, but its analysis process takes more time, because it involves more steps and demands more caution from laboratory technicians. Despite its higher cost, about six times higher than the physicalchemical method, the IAC method showed to be faster and easier to perform. KEY-WORDS: Recovery; aflatoxins; extraction methods; immunoaffinity; HPLC. Foi realizada avaliação comparativa de dois métodos de extração, limpeza e purificação de aflatoxinas, para análise em cromatógrafo líquido de alta eficiência. Um dos métodos é físicoquímico e baseia-se em reações químicas para todas as etapas. O outro utiliza coluna de imunoafinidade. Os métodos foram avaliados quanto à porcentagem de recuperação, custo do processo, tempo gasto para análise e número de amostras analisadas por dia. A recuperação obtida pelo método físicoquímico foi mais baixa do que a do método de imunoafinidade, para as aflatoxinas B1, B2 e G1, porém mais alta para G2. Ambos os métodos apresentaram valores de recuperação dentro da faixa estabelecida pela Comunidade Européia, que vai de 70% a 120%. A análise comparativa de custos revelou que estes foram menores no método físico-químico. Porém, suas análises são demoradas, por envolverem várias etapas, e exigem maior cautela por parte do laboratorista. Apesar do custo mais elevado, aproximadamente seis vezes superior ao do método físicoquímico, o método de imunoafinidade apresenta maior rapidez e facilidade de execução. PALAVRAS-CHAVE: Recuperação; Aflatoxinas; métodos de extração; imunoafinidade; CLAE.Escola de Agronomia - Universidade Federal de Goiás2008-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa Experimentaapplication/pdfhttps://revistas.ufg.br/pat/article/view/4158Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 38, n. 2, Apr./Jun. 2008; 103-108Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 38, n. 2, abr./jun. 2008; 103-108Pesquisa Agropecuária Tropical; v. 38, n. 2, abr./jun. 2008; 103-1081983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/pat/article/view/4158/3657Ramos, Cristiane ReginaBrasil, Edward MadureiraGeraldine, Robson Maiainfo:eu-repo/semantics/openAccess2020-02-27T00:02:32Zoai:ojs.revistas.ufg.br:article/4158Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:55:38.099076Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
AVALIAÇÃO DE MÉTODOS DE EXTRAÇÃO, LIMPEZA E PURIFICAÇÃO DE AFLATOXINAS PARA ANÁLISE EM CROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIA
title EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
spellingShingle EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
Ramos, Cristiane Regina
Recovery
aflatoxins
extraction methods
immunoaffinity
HPLC
Recuperação
aflatoxinas
métodos de extração
imunoafinidade
CLAE
title_short EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
title_full EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
title_fullStr EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
title_full_unstemmed EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
title_sort EVALUATION OF EXTRACTION, CLEANING AND PURIFICATION METHODS OF AFLATOXINS FOR HPLC ANALYSIS
author Ramos, Cristiane Regina
author_facet Ramos, Cristiane Regina
Brasil, Edward Madureira
Geraldine, Robson Maia
author_role author
author2 Brasil, Edward Madureira
Geraldine, Robson Maia
author2_role author
author
dc.contributor.author.fl_str_mv Ramos, Cristiane Regina
Brasil, Edward Madureira
Geraldine, Robson Maia
dc.subject.por.fl_str_mv Recovery
aflatoxins
extraction methods
immunoaffinity
HPLC
Recuperação
aflatoxinas
métodos de extração
imunoafinidade
CLAE
topic Recovery
aflatoxins
extraction methods
immunoaffinity
HPLC
Recuperação
aflatoxinas
métodos de extração
imunoafinidade
CLAE
description A comparative evaluation process was carried out between two methods of aflatoxins extraction, cleaning, and purification, for High Performance Liquid Chromatography (HPLC) analysis. The first one is the physical-chemical method, based on chemical reactions for all the stages, and the second makes use of a immunoaffinity column (IAC). The methods were evaluated as related to recovery percentage, cost, time spent for analysis, and number of samples analyzed per day. The recovery percentage obtained through the physical-chemical method was lower than the IAC method to B1, B2 and G1 aflatoxins, and higher to G2 aflatoxin. Both techniques presented recovery percentages in accordance with established values by the European Community (70% to 120%). Concerning costs, the physical-chemical method showed to be the least expensive, but its analysis process takes more time, because it involves more steps and demands more caution from laboratory technicians. Despite its higher cost, about six times higher than the physicalchemical method, the IAC method showed to be faster and easier to perform. KEY-WORDS: Recovery; aflatoxins; extraction methods; immunoaffinity; HPLC.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa Experimenta
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/4158
url https://revistas.ufg.br/pat/article/view/4158
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/4158/3657
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 38, n. 2, Apr./Jun. 2008; 103-108
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 38, n. 2, abr./jun. 2008; 103-108
Pesquisa Agropecuária Tropical; v. 38, n. 2, abr./jun. 2008; 103-108
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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