CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES

Detalhes bibliográficos
Autor(a) principal: Lima, Juliana Domingues
Data de Publicação: 2007
Outros Autores: Moraes, Wilson da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/2164
Resumo: The purpose of this study was to evaluate a protocol for in vitro multiplication of four Musa spp. genotypes. Previous tests indicated the utilization of an ideal benzilaminopurin concentration of 5.0 mg L-1; 5.0 mg L-1; 4.5 mg L-1 e 4.0 mg L-1 in culture medium MS, during the multiplication of Caipira, Thap maeo, PV03-44 and FHIA-01 genotypes, respectively. After that, the multiplication rate, the size of shoots produced per explant, fungal and bacterial contamination rate, oxidation rate, and abnormality occurrence in the multiplication phases were evaluated. The multiplication rate and the size of shoots produced per explant varied among genotypes in the five subcultures studied. The highest contamination rate was caused by bacteria and was more frequent in the in vitro establishment, tending to be reduced with the number of subcultures. The most of the genotypes did not present problems of microorganism contamination, except for Caipira cultivar which presented high bacterial contamination rate during in vitro establishment. This problem is a limitation of the protocol to obtain micropropagated plantlets of this genotype. KEY-WORDS: Musa spp.; micropropagation; tissue culture; benzilaminopurin.
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spelling CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPESCONCENTRAÇÃO DE BENZILAMINOPURINA E AVALIAÇÃO DE PROTOCOLO PARA MULTIPLICAÇÃO IN VITRO DE GENÓTIPOS DE BANANEIRAMusa spp.micropropagationtissue culturebenzilaminopurinMusa spp.micropropagaçãocultura de tecidosbenzilaminopurina The purpose of this study was to evaluate a protocol for in vitro multiplication of four Musa spp. genotypes. Previous tests indicated the utilization of an ideal benzilaminopurin concentration of 5.0 mg L-1; 5.0 mg L-1; 4.5 mg L-1 e 4.0 mg L-1 in culture medium MS, during the multiplication of Caipira, Thap maeo, PV03-44 and FHIA-01 genotypes, respectively. After that, the multiplication rate, the size of shoots produced per explant, fungal and bacterial contamination rate, oxidation rate, and abnormality occurrence in the multiplication phases were evaluated. The multiplication rate and the size of shoots produced per explant varied among genotypes in the five subcultures studied. The highest contamination rate was caused by bacteria and was more frequent in the in vitro establishment, tending to be reduced with the number of subcultures. The most of the genotypes did not present problems of microorganism contamination, except for Caipira cultivar which presented high bacterial contamination rate during in vitro establishment. This problem is a limitation of the protocol to obtain micropropagated plantlets of this genotype. KEY-WORDS: Musa spp.; micropropagation; tissue culture; benzilaminopurin. Este trabalho teve por objetivo avaliar um protocolo para multiplicação in vitro para quatro genótipos de bananeira (Musa spp.) em escala comercial. Testes preliminares indicaram a utilização das concentrações ótimas de 5,0 mg L-1; 5,0 mg L-1; 4,5 mg L-1 e 4,0 mg L-1 de benzilaminopurina no meio de cultura MS, para a multiplicação dos genótipos Caipira, Thap maeo, PV03- 44 e FHIA-01, respectivamente. Em seguida, avaliou-se a taxa de multiplicação, o tamanho de brotos produzidos por explante, a taxa de contaminação por fungos e bactérias, a taxa de oxidação e a ocorrência de anormalidades durante as fases da multiplicação. A taxa de multiplicação e o tamanho de brotos por explante variou entre genótipos nos cinco subcultivos realizados. A taxa de contaminação mais elevada foi de origem bacteriana e mais freqüente na fase de estabelecimento in vitro, com tendência de redução à medida que foram realizados os subcultivos. A maioria dos genótipos não apresentou problemas de contaminação por microorganismos, exceto a cultivar Caipira que apresentou elevada taxa de contaminação por bactérias na fase de estabelecimento in vitro, o que representa uma limitação do protocolo para a multiplicação comercial desse genótipo. PALAVRAS-CHAVE: Musa spp.; micropropagação; cultura de tecidos; benzilaminopurina.Escola de Agronomia - Universidade Federal de Goiás2007-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa de Laboratórioapplication/pdfhttps://revistas.ufg.br/pat/article/view/2164Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 36, n. 1, Jan./Apr. 2006; 13-19Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 36, n. 1, jan./abr. 2006; 13-19Pesquisa Agropecuária Tropical; v. 36, n. 1, jan./abr. 2006; 13-191983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/pat/article/view/2164/2115Lima, Juliana DominguesMoraes, Wilson da Silvainfo:eu-repo/semantics/openAccess2010-02-19T11:32:16Zoai:ojs.revistas.ufg.br:article/2164Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:54:57.003017Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
CONCENTRAÇÃO DE BENZILAMINOPURINA E AVALIAÇÃO DE PROTOCOLO PARA MULTIPLICAÇÃO IN VITRO DE GENÓTIPOS DE BANANEIRA
title CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
spellingShingle CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
Lima, Juliana Domingues
Musa spp.
micropropagation
tissue culture
benzilaminopurin
Musa spp.
micropropagação
cultura de tecidos
benzilaminopurina
title_short CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
title_full CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
title_fullStr CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
title_full_unstemmed CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
title_sort CONCENTRATION OF BAP AND EVALUATION OF IN VITRO MULTIPLICATION PROTOCOL FOR BANANA GENOTYPES
author Lima, Juliana Domingues
author_facet Lima, Juliana Domingues
Moraes, Wilson da Silva
author_role author
author2 Moraes, Wilson da Silva
author2_role author
dc.contributor.author.fl_str_mv Lima, Juliana Domingues
Moraes, Wilson da Silva
dc.subject.por.fl_str_mv Musa spp.
micropropagation
tissue culture
benzilaminopurin
Musa spp.
micropropagação
cultura de tecidos
benzilaminopurina
topic Musa spp.
micropropagation
tissue culture
benzilaminopurin
Musa spp.
micropropagação
cultura de tecidos
benzilaminopurina
description The purpose of this study was to evaluate a protocol for in vitro multiplication of four Musa spp. genotypes. Previous tests indicated the utilization of an ideal benzilaminopurin concentration of 5.0 mg L-1; 5.0 mg L-1; 4.5 mg L-1 e 4.0 mg L-1 in culture medium MS, during the multiplication of Caipira, Thap maeo, PV03-44 and FHIA-01 genotypes, respectively. After that, the multiplication rate, the size of shoots produced per explant, fungal and bacterial contamination rate, oxidation rate, and abnormality occurrence in the multiplication phases were evaluated. The multiplication rate and the size of shoots produced per explant varied among genotypes in the five subcultures studied. The highest contamination rate was caused by bacteria and was more frequent in the in vitro establishment, tending to be reduced with the number of subcultures. The most of the genotypes did not present problems of microorganism contamination, except for Caipira cultivar which presented high bacterial contamination rate during in vitro establishment. This problem is a limitation of the protocol to obtain micropropagated plantlets of this genotype. KEY-WORDS: Musa spp.; micropropagation; tissue culture; benzilaminopurin.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa de Laboratório
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/2164
url https://revistas.ufg.br/pat/article/view/2164
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/2164/2115
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 36, n. 1, Jan./Apr. 2006; 13-19
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 36, n. 1, jan./abr. 2006; 13-19
Pesquisa Agropecuária Tropical; v. 36, n. 1, jan./abr. 2006; 13-19
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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