Efeito do BAP no cultivo in vitro de Ocimum selloi Benth

Detalhes bibliográficos
Autor(a) principal: Monfort, L. E. F.
Data de Publicação: 2012
Outros Autores: Pinto, J. E. B. P., Bertolucci, S. K. V., Rossi, Z. T. T., Santos, F. M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38317
Resumo: The present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment) and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1). After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the tested conditions, use of the first and the second nodal segments is recommended in addition to supplementing the culture medium with BAP for in vitro proliferation of O. selloi sprouts.
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spelling Efeito do BAP no cultivo in vitro de Ocimum selloi BenthEffect of BAP on in vitro culture of Ocimum selloi BenthPlantas medicinaisBenzilaminopurinaAtroveran - MicropropagationMedicinal plantsBenzylaminopurineAtroveran - MicropropagaçãoThe present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment) and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1). After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the tested conditions, use of the first and the second nodal segments is recommended in addition to supplementing the culture medium with BAP for in vitro proliferation of O. selloi sprouts.Visando promover a proliferação de brotações em segmentos apicais e nodais de Ocimum selloi em diferentes concentrações de BAP, plantas jovens de 60 dias serviram de doadoras de segmentos apicais e nodais. Os segmentos foram inoculados em meio MS preparado com a metade da concentração dos sais, e acrescido de 1,5% de sacarose e diferentes concentrações de BAP. O experimento foi conduzido no delineamento fatorial, 3 x 4, sendo 3 as posições dos segmentos de O. selloi (segmento apical, primeiro e segundo segmentos nodais) e 4 concentrações de BAP (0 - controle; 2; 4 e 6 mg L-1). Aos 30 dias, foram avaliados o número, comprimento e biomassa fresca e seca de brotos e raízes. Os primeiros e segundos segmentos apresentaram melhores resultados na indução de brotos de O. selloi, 7 e 8 brotos/explante, nas diferentes concentrações de BAP; porém, não houve formação de raízes na presença da citocinina. Nas condições testadas, recomenda-se o uso do primeiro e segundo segmento nodal suplementando o meio de cultivo com BAP para a proliferação in vitro de brotações de O. selloi.Sociedade Brasileira de Plantas Medicinais2019-12-19T17:12:45Z2019-12-19T17:12:45Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMONFORT, L. E. F.; PINTO, J. E. P. B.; BERTOLUCCI, S. K. V.; ROSSI, Z. T. T.; SANTOS, F. M. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth. Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 3, p. 458-463, 2012.http://repositorio.ufla.br/jspui/handle/1/38317Revista Brasileira de Plantas Medicinaisreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMonfort, L. E. F.Pinto, J. E. B. P.Bertolucci, S. K. V.Rossi, Z. T. T.Santos, F. M.por2023-05-26T18:51:18Zoai:localhost:1/38317Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:51:18Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
Effect of BAP on in vitro culture of Ocimum selloi Benth
title Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
spellingShingle Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
Monfort, L. E. F.
Plantas medicinais
Benzilaminopurina
Atroveran - Micropropagation
Medicinal plants
Benzylaminopurine
Atroveran - Micropropagação
title_short Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
title_full Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
title_fullStr Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
title_full_unstemmed Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
title_sort Efeito do BAP no cultivo in vitro de Ocimum selloi Benth
author Monfort, L. E. F.
author_facet Monfort, L. E. F.
Pinto, J. E. B. P.
Bertolucci, S. K. V.
Rossi, Z. T. T.
Santos, F. M.
author_role author
author2 Pinto, J. E. B. P.
Bertolucci, S. K. V.
Rossi, Z. T. T.
Santos, F. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Monfort, L. E. F.
Pinto, J. E. B. P.
Bertolucci, S. K. V.
Rossi, Z. T. T.
Santos, F. M.
dc.subject.por.fl_str_mv Plantas medicinais
Benzilaminopurina
Atroveran - Micropropagation
Medicinal plants
Benzylaminopurine
Atroveran - Micropropagação
topic Plantas medicinais
Benzilaminopurina
Atroveran - Micropropagation
Medicinal plants
Benzylaminopurine
Atroveran - Micropropagação
description The present study was undertaken to develop the proliferation of sprouts in apical and nodal segments of Ocimum selloi with different BAP levels. Young plants aged 60 days were used as donors of nodal and apical segments. The segments were inoculated in MS medium at half the concentration of salts supplemented with 1.5% of sucrose and different BAP levels. The experiment was in 3 x 4 factorial arrangement, 3 positions of O. selloi segments (apical segment; first and second nodal segment) and 3 BAP levels (0 - control; 2; 4 and 6 mg L-1). After 30 days, the number, the length, and the fresh and dry biomass of sprouts and roots were evaluated. The first and the second segments showed better results in inducing O. selloi sprouts, 7 and 8 sprouts/explant, at the different BAP levels, but there was not root formation in the presence of the cytokinin. Under the tested conditions, use of the first and the second nodal segments is recommended in addition to supplementing the culture medium with BAP for in vitro proliferation of O. selloi sprouts.
publishDate 2012
dc.date.none.fl_str_mv 2012
2019-12-19T17:12:45Z
2019-12-19T17:12:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MONFORT, L. E. F.; PINTO, J. E. P. B.; BERTOLUCCI, S. K. V.; ROSSI, Z. T. T.; SANTOS, F. M. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth. Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 3, p. 458-463, 2012.
http://repositorio.ufla.br/jspui/handle/1/38317
identifier_str_mv MONFORT, L. E. F.; PINTO, J. E. P. B.; BERTOLUCCI, S. K. V.; ROSSI, Z. T. T.; SANTOS, F. M. Efeito do BAP no cultivo in vitro de Ocimum selloi Benth. Revista Brasileira de Plantas Medicinais, Botucatu, v. 14, n. 3, p. 458-463, 2012.
url http://repositorio.ufla.br/jspui/handle/1/38317
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Plantas Medicinais
publisher.none.fl_str_mv Sociedade Brasileira de Plantas Medicinais
dc.source.none.fl_str_mv Revista Brasileira de Plantas Medicinais
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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