Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES

Detalhes bibliográficos
Autor(a) principal: Dias Filho, Francisco de Carvalho
Data de Publicação: 2008
Outros Autores: Linhares, Guido Fontgalland Coelho, Duarte, Sabrina Castilho, Quaiotti, Sônia Maria Santana, Jorge, Paula Marina Brito
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/4240
Resumo: This study was conducted with the objective of obtaining pure autochthonous isolates of Anaplasma marginale from blood samples of bovines that have been raised in the tick-borne disease endemic area of the municipality of Goiânia. The inoculum of A. marginale was prepared from a donor animal, known to be natural carrier of hemoparasite mixed infections, after treatment with a babesicid sterilizing dose of imidocarb dipropionate. A 20 mL blood sample was collected from the donor animal 2 days post-infection and then transferred to a colostrum-deprived and hemoparasite free newborn calf. The receptor animal showed signs of fever and apathy with patent A. marginale parasitemia 30 days after inoculation. Blood sample was then collected and prepared as stabilates to be cryopreserved and to take part of an autochthonous blood parasites isolates library. The isolate purity was assured concomitantly by specific seroconversion tests, by PCR and by finally by the subinoculation of susceptible calves with the cryopreseved stabilates. It was confirmed that the use of colostrum-deprived free newborn calves as susceptible animals may be considered a practical and effective model to obtain pure hemoparasite isolates in endemic areas with additional advantages such as feasibility and low cost. Key words: Anaplasma marginale, isolates, calves, bovine anaplasmosis.
id UFG-7_24e7fd2137cb63b5de4d3a93d61aebd2
oai_identifier_str oai:ojs.revistas.ufg.br:article/4240
network_acronym_str UFG-7
network_name_str Ciência animal brasileira (Online)
repository_id_str
spelling Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVESOBTENÇÃO DE ISOLADO PURO DE Anaplasma marginale EM BEZERROS NEONATOS PRIVADOS DE COLOSTROSanidade AnimalThis study was conducted with the objective of obtaining pure autochthonous isolates of Anaplasma marginale from blood samples of bovines that have been raised in the tick-borne disease endemic area of the municipality of Goiânia. The inoculum of A. marginale was prepared from a donor animal, known to be natural carrier of hemoparasite mixed infections, after treatment with a babesicid sterilizing dose of imidocarb dipropionate. A 20 mL blood sample was collected from the donor animal 2 days post-infection and then transferred to a colostrum-deprived and hemoparasite free newborn calf. The receptor animal showed signs of fever and apathy with patent A. marginale parasitemia 30 days after inoculation. Blood sample was then collected and prepared as stabilates to be cryopreserved and to take part of an autochthonous blood parasites isolates library. The isolate purity was assured concomitantly by specific seroconversion tests, by PCR and by finally by the subinoculation of susceptible calves with the cryopreseved stabilates. It was confirmed that the use of colostrum-deprived free newborn calves as susceptible animals may be considered a practical and effective model to obtain pure hemoparasite isolates in endemic areas with additional advantages such as feasibility and low cost. Key words: Anaplasma marginale, isolates, calves, bovine anaplasmosis.O trabalho foi realizado com o objetivo principal de obter isolados autóctones puros de Anaplasma marginale a partir de amostra de sangue de bovino criado em uma área endêmica para a tristeza parasitária bovina no município de Goiânia. Obteve-se o inóculo para o isolamento de A. marginale de animal doador, portador de infecções naturais mistas, após receber tratamento seletivo com dose babesicida esterelizante de dipropionato de imidocarb. Dois dias após esse tratamento, colheu-se dele uma amostra de sangue de 20 mL do animal doador para inoculação em bezerro neonato privado de colostro e livre de infecções por hemoparasitos. Após trinta dias da inoculação, o receptor apresentou febre, apatia e parasitemia patente por A. marginale. Amostras de sangue foram então colhidas e preparadas na forma de estabilizados para serem criopreservadas e, assim, comporem banco de isolados de hemoparasitos autóctones. Realizou-se a comprovação da pureza do isolado, concomitantemente, pela demonstração de soroconversão específica, pela reação de PCR e, ainda, pela subinoculação da amostra criopreservada em bezerro suscetível. Constatou-se ainda que o uso de bezerros neonatos privados de colostro, como animais suscetíveis, pode ser considerado como modelo prático, eficaz e relativamente barato para o isolamento de hemoparasitos em regiões endêmicas. PALAVRAS-CHAVES: Anaplasma marginale, anaplasmose bovina, bezerros, isolados.Universidade Federal de Goiás2008-07-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisaapplication/pdfhttps://revistas.ufg.br/vet/article/view/424010.5216/cab.v9i2.4240Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 2 (2008); 390-395Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 2 (2008); 390-3951809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/4240/3715Dias Filho, Francisco de CarvalhoLinhares, Guido Fontgalland CoelhoDuarte, Sabrina CastilhoQuaiotti, Sônia Maria SantanaJorge, Paula Marina Britoinfo:eu-repo/semantics/openAccess2008-07-10T00:26:37Zoai:ojs.revistas.ufg.br:article/4240Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:13.918570Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
OBTENÇÃO DE ISOLADO PURO DE Anaplasma marginale EM BEZERROS NEONATOS PRIVADOS DE COLOSTRO
title Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
spellingShingle Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
Dias Filho, Francisco de Carvalho
Sanidade Animal
title_short Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
title_full Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
title_fullStr Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
title_full_unstemmed Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
title_sort Anaplasma marginale PURE ISOLATE OBTAINED IN COLOSTRUM-DEPRIVED NEWBORN CALVES
author Dias Filho, Francisco de Carvalho
author_facet Dias Filho, Francisco de Carvalho
Linhares, Guido Fontgalland Coelho
Duarte, Sabrina Castilho
Quaiotti, Sônia Maria Santana
Jorge, Paula Marina Brito
author_role author
author2 Linhares, Guido Fontgalland Coelho
Duarte, Sabrina Castilho
Quaiotti, Sônia Maria Santana
Jorge, Paula Marina Brito
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Dias Filho, Francisco de Carvalho
Linhares, Guido Fontgalland Coelho
Duarte, Sabrina Castilho
Quaiotti, Sônia Maria Santana
Jorge, Paula Marina Brito
dc.subject.por.fl_str_mv Sanidade Animal
topic Sanidade Animal
description This study was conducted with the objective of obtaining pure autochthonous isolates of Anaplasma marginale from blood samples of bovines that have been raised in the tick-borne disease endemic area of the municipality of Goiânia. The inoculum of A. marginale was prepared from a donor animal, known to be natural carrier of hemoparasite mixed infections, after treatment with a babesicid sterilizing dose of imidocarb dipropionate. A 20 mL blood sample was collected from the donor animal 2 days post-infection and then transferred to a colostrum-deprived and hemoparasite free newborn calf. The receptor animal showed signs of fever and apathy with patent A. marginale parasitemia 30 days after inoculation. Blood sample was then collected and prepared as stabilates to be cryopreserved and to take part of an autochthonous blood parasites isolates library. The isolate purity was assured concomitantly by specific seroconversion tests, by PCR and by finally by the subinoculation of susceptible calves with the cryopreseved stabilates. It was confirmed that the use of colostrum-deprived free newborn calves as susceptible animals may be considered a practical and effective model to obtain pure hemoparasite isolates in endemic areas with additional advantages such as feasibility and low cost. Key words: Anaplasma marginale, isolates, calves, bovine anaplasmosis.
publishDate 2008
dc.date.none.fl_str_mv 2008-07-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/4240
10.5216/cab.v9i2.4240
url https://revistas.ufg.br/vet/article/view/4240
identifier_str_mv 10.5216/cab.v9i2.4240
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/4240/3715
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 2 (2008); 390-395
Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 2 (2008); 390-395
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874784989806592