Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/58640 |
Resumo: | The aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution.Keywords: dog, mammary gland, neoplasm, matrix metalloproteinases. |
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Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissueImunomarcação de metaloproteinases 9 (MMP-9) e 2 (MMP-2) e seus inibidores (TIMP-1) e (TIMP-2) no tecido mamário canino normal e neoplásicoThe aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution.Keywords: dog, mammary gland, neoplasm, matrix metalloproteinases.Este estudo teve como objetivo realizar a imunomarcação de MMP-9 e MMP-2 e seus inibidores, TIMP-1 e TIMP-2, no tecido mamário canino normal e neoplásico, a fim de avaliar o comportamento dessas proteínas no remodelamento da matriz extracelular (MEC) em diferentes tipos neoplásicos mamários. Foram analisadas 48 amostras de tecido mamário canino, sendo 14 carcinomas complexos, 13 carcinomas tubulopapilares, seis de adenomas simples e 15 mamas sem alterações. Houve diferença em MMP-9, TIMP-1 e TIMP-2 de acordo com a histomorfologia mamária, sendo que MMP-9 apresentou maior imunoexpressão em células epiteliais e estromais em carcinomas tubulopapilares e complexos. TIMP-1 exibiu menor imunomarcação nas células estromais dos carcinomas complexos e TIMP-2 maior imunomarcação nas células epiteliais dos carcinomas tubulopapilares. Houve correlação positiva entre MMP-9 e TIMP-1 nas células epiteliais e estromais quanto à intensidade de imunomarcação e número de células marcadas na mama normal. Houve correlação positiva entre MMP-9 e TIMP-2 nas células epiteliais dos carcinomas tubulopapilares. Conclui-se que a atividade equilibrada entre MMP-9, MMP-2, TIMP-1 e TIMP-2 mantém a homeostase do tecido mamário canino normal enquanto a imunoexpressão aumentada de MMP-9 e TIMP-2 e a imunoexpressão reduzida de TIMP-1 nos carcinomas sugere condição propícia à evolução tumoral.Palavras-chave: cão, glândula mamária, neoplasia, metaloproteinases de matriz.Universidade Federal de Goiás2020-08-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/58640Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 21 (2020): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 21 (2020): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/58640/35218https://revistas.ufg.br/vet/article/view/58640/35219https://revistas.ufg.br/vet/article/view/58640/35220https://revistas.ufg.br/vet/article/view/58640/35221Copyright (c) 2020 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessFerreira, Hugo HenriqueToledo, Denise CarolineFaria, Adriana MarquesAraújo, Diego Pereiradi Nardi, Andrigo BarbozaPorto, Regiani Nascimento GagnoMoura, Veridiana Maria Brianezi Dignani de2023-03-23T18:46:38Zoai:ojs.revistas.ufg.br:article/58640Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:25.230093Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue Imunomarcação de metaloproteinases 9 (MMP-9) e 2 (MMP-2) e seus inibidores (TIMP-1) e (TIMP-2) no tecido mamário canino normal e neoplásico |
title |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
spellingShingle |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue Ferreira, Hugo Henrique |
title_short |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_full |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_fullStr |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_full_unstemmed |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
title_sort |
Immunoexpression of metalloproteinases 9 (MMP-9) and 2 (MMP-2) and their inhibitors (TIMP-1 and TIMP-2) in normal and neoplastic canine mammary tissue |
author |
Ferreira, Hugo Henrique |
author_facet |
Ferreira, Hugo Henrique Toledo, Denise Caroline Faria, Adriana Marques Araújo, Diego Pereira di Nardi, Andrigo Barboza Porto, Regiani Nascimento Gagno Moura, Veridiana Maria Brianezi Dignani de |
author_role |
author |
author2 |
Toledo, Denise Caroline Faria, Adriana Marques Araújo, Diego Pereira di Nardi, Andrigo Barboza Porto, Regiani Nascimento Gagno Moura, Veridiana Maria Brianezi Dignani de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Hugo Henrique Toledo, Denise Caroline Faria, Adriana Marques Araújo, Diego Pereira di Nardi, Andrigo Barboza Porto, Regiani Nascimento Gagno Moura, Veridiana Maria Brianezi Dignani de |
description |
The aim of this study was to perform the immunostaining of MMP-9 and MMP-2 and its inhibitors, TIMP-1 and TIMP-2, on normal and neoplastic canine mammary tissue in order to evaluate the behavior of these proteins in extracellular matrix (ECM) remodeling in different neoplastic mammary types. Thus, 48 samples of canine mammary tissue were analyzed, 14 of which complex carcinomas, 13 tubulopapillary carcinomas, six single adenomas and 15 normal mammary tissue. There were differences in MMP-9, TIMP-1 and TIMP-2 according to mammary histomorphology, and MMP-9 presented increased immunoexpression in epithelial and stromal cells in tubulopapillary and complex carcinomas. TIMP-1 exhibited reduced immunostaining in the stromal cells of the complex carcinomas and TIMP-2 enhanced immunostaining in the epithelial cells of tubulopapillary carcinomas. There was a positive correlation between MMP-9 and TIMP-1 in epithelial and stromal cells regarding immunostaining intensity and number of labeled cells in the normal breast. There was a positive correlation between MMP-9 and TIMP-2 in the epithelial cells of tubulopapillary carcinomas. It is concluded that balanced activity between MMP-9, MMP-2, TIMP-1 and TIMP-2 maintains normal canine mammary tissue homeostasis while increased immunoexpression of MMP-9 and TIMP-2 and reduced TIMP- 1 in carcinomas suggest a favorable condition for tumor evolution.Keywords: dog, mammary gland, neoplasm, matrix metalloproteinases. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/58640 |
url |
https://revistas.ufg.br/vet/article/view/58640 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/58640/35218 https://revistas.ufg.br/vet/article/view/58640/35219 https://revistas.ufg.br/vet/article/view/58640/35220 https://revistas.ufg.br/vet/article/view/58640/35221 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Ciência Animal Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Ciência Animal Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 21 (2020): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 21 (2020): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874790057574400 |