SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES

• Autor(a) principal: Tongu, Eriky Akio de Oliveira
• Data de Publicação: 2015
• Outros Autores: Taborda, Carlos Peleschi, Marques, Shirlei Aparecida Vieira, Gomes, Gustavo Mendes, Gomes, Letícia Patrão de Macedo, Peixoto Jr., Kleber da Cunha, Crespilho, André Maciel
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/31444
• topic: Animal Reproduction; Reprodução Animal; Sanidade Animal; Clínica Médica de Equinos

Among different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility.Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originally developed for the isolation of fungi that concerns Human Medicine. In Experiment 1, standard(ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completionof uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopicobservation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2,five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowingin Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterizedby microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determiningspecificity of 85%. However, the results did not compromise the efficiency of Dermatobac® systemgiven all contaminants presented differentiated morphology and higher growing time (P<0.05)when compared to the major etiological agents of equine fungal endometritis. The Dermatobac®system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showingconclusive information only after 24 hours of culture.Keywords: Dermatobac®; diagnosis; endometritis; fungi; mare.