SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/31444 |
Resumo: | Among different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility.Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originally developed for the isolation of fungi that concerns Human Medicine. In Experiment 1, standard(ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completionof uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopicobservation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2,five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowingin Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterizedby microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determiningspecificity of 85%. However, the results did not compromise the efficiency of Dermatobac® systemgiven all contaminants presented differentiated morphology and higher growing time (P<0.05)when compared to the major etiological agents of equine fungal endometritis. The Dermatobac®system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showingconclusive information only after 24 hours of culture.Keywords: Dermatobac®; diagnosis; endometritis; fungi; mare. |
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SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARESSENSIBILIDADE DO LAMINOCULTIVO DERMATOBAC® PARA O DIAGNÓSTICO DA ENDOMETRITE FÚNGICA EM ÉGUASAnimal ReproductionReprodução AnimalSanidade AnimalClínica Médica de EquinosAmong different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility.Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originally developed for the isolation of fungi that concerns Human Medicine. In Experiment 1, standard(ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completionof uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopicobservation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2,five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowingin Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterizedby microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determiningspecificity of 85%. However, the results did not compromise the efficiency of Dermatobac® systemgiven all contaminants presented differentiated morphology and higher growing time (P<0.05)when compared to the major etiological agents of equine fungal endometritis. The Dermatobac®system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showingconclusive information only after 24 hours of culture.Keywords: Dermatobac®; diagnosis; endometritis; fungi; mare.Dentre os diferentes fatores etiológicos, a endometrite fúngica representa uma causa frequente deinfertilidade na égua. Nesse sentido, dois experimentos foram realizados para avaliar a sensibilidadedo Dermatobac®, sistema originalmente desenvolvido para o isolamento de fungos de interesse emMedicina Humana. No Experimento 1, cepas padrão de Candida (n=4) e Aspergyllus (n=1) foramsemeadas assepticamente e em duplicata no sistema Dermatobac®, acompanhando-se o crescimentofúngico a cada 24 horas por 7 dias. Para o Experimento 2, foram selecionadas 34 éguas, submetidasà colheita de material uterino para o plaqueamento em Dermatobac® e realização de esfregaços parapesquisa direta de fungos (Controle). No Experimento 1, foi possível a observação macroscópica detodas as cepas de Candida e Aspergyllus a partir de 24 horas do início do cultivo microbiológico.No Experimento 2, foi constatada a presença de leveduras nas lâminas citológicas de cinco animais(14,71%), resultados também confirmados na semeadura em Dermatobac® (sensibilidade de 100%).Foram observados cinco casos falso-positivos caracterizados pelo crescimento microbiológico emsistema Dermatobac® não confirmados através da citologia endometrial, resultados que determinarama especificidade de 85%. No entanto, todos os casos falso-positivos foram atribuídos a microrganismoscontaminantes que apresentaram morfologia diferenciada e tempo de crescimento superior (P<0,05)ao apresentado pelos principais agentes etiológicos das endometrites fúngicas equinas. Conclui-seque o sistema Dermatobac® é eficiente para o diagnóstico macroscópico da endometrite fúngica emequinos, gerando informações visuais a partir de 24 horas de cultivo.Palavras-Chave: Dermatobac®; diagnóstico; éguas; endometrite; fungos.Universidade Federal de Goiás2015-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/3144410.1590/cab.v16i4.31444Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 4 (2015); 598-607Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 4 (2015); 598-6071809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/31444/19470https://revistas.ufg.br/vet/article/view/31444/19471Tongu, Eriky Akio de OliveiraTaborda, Carlos PeleschiMarques, Shirlei Aparecida VieiraGomes, Gustavo MendesGomes, Letícia Patrão de MacedoPeixoto Jr., Kleber da CunhaCrespilho, André Macielinfo:eu-repo/semantics/openAccess2015-11-10T17:44:06Zoai:ojs.revistas.ufg.br:article/31444Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:06.999894Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES SENSIBILIDADE DO LAMINOCULTIVO DERMATOBAC® PARA O DIAGNÓSTICO DA ENDOMETRITE FÚNGICA EM ÉGUAS |
title |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES |
spellingShingle |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES Tongu, Eriky Akio de Oliveira Animal Reproduction Reprodução Animal Sanidade Animal Clínica Médica de Equinos |
title_short |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES |
title_full |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES |
title_fullStr |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES |
title_full_unstemmed |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES |
title_sort |
SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES |
author |
Tongu, Eriky Akio de Oliveira |
author_facet |
Tongu, Eriky Akio de Oliveira Taborda, Carlos Peleschi Marques, Shirlei Aparecida Vieira Gomes, Gustavo Mendes Gomes, Letícia Patrão de Macedo Peixoto Jr., Kleber da Cunha Crespilho, André Maciel |
author_role |
author |
author2 |
Taborda, Carlos Peleschi Marques, Shirlei Aparecida Vieira Gomes, Gustavo Mendes Gomes, Letícia Patrão de Macedo Peixoto Jr., Kleber da Cunha Crespilho, André Maciel |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Tongu, Eriky Akio de Oliveira Taborda, Carlos Peleschi Marques, Shirlei Aparecida Vieira Gomes, Gustavo Mendes Gomes, Letícia Patrão de Macedo Peixoto Jr., Kleber da Cunha Crespilho, André Maciel |
dc.subject.por.fl_str_mv |
Animal Reproduction Reprodução Animal Sanidade Animal Clínica Médica de Equinos |
topic |
Animal Reproduction Reprodução Animal Sanidade Animal Clínica Médica de Equinos |
description |
Among different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility.Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originally developed for the isolation of fungi that concerns Human Medicine. In Experiment 1, standard(ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completionof uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopicobservation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2,five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowingin Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterizedby microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determiningspecificity of 85%. However, the results did not compromise the efficiency of Dermatobac® systemgiven all contaminants presented differentiated morphology and higher growing time (P<0.05)when compared to the major etiological agents of equine fungal endometritis. The Dermatobac®system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showingconclusive information only after 24 hours of culture.Keywords: Dermatobac®; diagnosis; endometritis; fungi; mare. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/31444 10.1590/cab.v16i4.31444 |
url |
https://revistas.ufg.br/vet/article/view/31444 |
identifier_str_mv |
10.1590/cab.v16i4.31444 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/31444/19470 https://revistas.ufg.br/vet/article/view/31444/19471 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 4 (2015); 598-607 Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 4 (2015); 598-607 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788697571328 |