Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL

Detalhes bibliográficos
Autor(a) principal: Rodrigues Poester, Vanice
Data de Publicação: 2018
Outros Autores: Furtado Mendes, Josiara, Von Groll, Andrea, Baracy Klafke, Gabriel, Martinez Brandolt, Tchana, Orzechowski Xavier, Melissa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/e-52571
Resumo: The increase in zoonotic sporotrichosis cases has become a public health problem in hyperendemic areas for the feline disease. Domestic cats involvement in this transmission and the continuous contact of these animals with soil suggest that the environment may be an essential source and/or reservoir of Sporothrix spp. Thus, the current study aimed to evaluate the presence of Sporothrix spp. in the soil of places where infected cats in Southern Brazil is present. In total, 101 soil samples were collected from the residence of cats with sporotrichosis, from regions around to these residences, and also from public places. These samples were processed using the direct plating technique being incubated at 25 °C for up to 15 days. Additionally, seventeen samples were also submitted to DNA extraction (NORGEN BIOTEK CORP(r) kit), PCR-specific and Nested-PCR technique. Although the presence of Sporothrix in soil has been undetected, by culture or molecular biology, this study does not reject the role of the environment in the triad of the infectious process (soil-animal-human). Thus, further studies are necessary for expanding the area of coverage, volume, type and method of sampling, and/or detection techniques.Keywords: Environment; Geophilic; S. brasiliensis; S. schenckii
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spelling Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZILAvaliação da presença de Sporothrix spp. Em solo de área hiperendêmica para esporotricose no extremo sul do BrasilThe increase in zoonotic sporotrichosis cases has become a public health problem in hyperendemic areas for the feline disease. Domestic cats involvement in this transmission and the continuous contact of these animals with soil suggest that the environment may be an essential source and/or reservoir of Sporothrix spp. Thus, the current study aimed to evaluate the presence of Sporothrix spp. in the soil of places where infected cats in Southern Brazil is present. In total, 101 soil samples were collected from the residence of cats with sporotrichosis, from regions around to these residences, and also from public places. These samples were processed using the direct plating technique being incubated at 25 °C for up to 15 days. Additionally, seventeen samples were also submitted to DNA extraction (NORGEN BIOTEK CORP(r) kit), PCR-specific and Nested-PCR technique. Although the presence of Sporothrix in soil has been undetected, by culture or molecular biology, this study does not reject the role of the environment in the triad of the infectious process (soil-animal-human). Thus, further studies are necessary for expanding the area of coverage, volume, type and method of sampling, and/or detection techniques.Keywords: Environment; Geophilic; S. brasiliensis; S. schenckiiO aumento de casos de esporotricose zoonótica tornou-se um problema de saúde pública em áreas hiperendêmicas para a doença em felinos. O envolvimento dos gatos domésticos nesta transmissão e o contato contínuo destes animais com o solo sugerem que o ambiente possa ser uma importante fonte e/ou reservatório de fungos Sporothrix spp. Assim, este estudo objetivou avaliar a presença de fungos do complexo Sporothrix em amostras de solo provenientes de locais de acesso de felinos infectados no sul do Brasil. Foram coletadas amostras de solo de residências com felinos com esporotricose, de locais próximos a estas residências e também de locais públicos, totalizando 101 amostras. Estas foram processadas pela técnica de plaqueamento direto e incubadas a 25°C por até 15 dias. Dezessete amostras foram paralelamente submetidas a extração de DNA (kitNORGEN BIOTEK CORP(r)) e técnica de PCR espécie-específico e Nested-PCR. Embora em nenhuma amostra avaliada tenha sido detectada a presença de Sporothrix spp., seja por cultivo ou por biologia molecular, este estudo não descarta o papel do ambiente na tríade do processo infeccioso (solo - animal - humano), sendo necessários outros estudos ampliando área de abrangência, volume, tipo e método de coleta das amostras, bem como técnicas de detecção.Palavras-chave: Ambiente; Geofílico; S. brasiliensis; S. schenckiiUniversidade Federal de Goiás2018-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-5257110.1590/cab19052571Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-8Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-81809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-52571/26515https://revistas.ufg.br/vet/article/view/e-52571/26516Copyright (c) 2018 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessRodrigues Poester, VaniceFurtado Mendes, JosiaraVon Groll, AndreaBaracy Klafke, GabrielMartinez Brandolt, TchanaOrzechowski Xavier, Melissa2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/52571Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:22.587991Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
Avaliação da presença de Sporothrix spp. Em solo de área hiperendêmica para esporotricose no extremo sul do Brasil
title Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
spellingShingle Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
Rodrigues Poester, Vanice
title_short Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
title_full Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
title_fullStr Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
title_full_unstemmed Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
title_sort Sporothrix spp. EVALUATION IN SOIL OF A HYPERENDENIC AREA FOR SPOROTRICHOSIS IN SOUTHERN BRAZIL
author Rodrigues Poester, Vanice
author_facet Rodrigues Poester, Vanice
Furtado Mendes, Josiara
Von Groll, Andrea
Baracy Klafke, Gabriel
Martinez Brandolt, Tchana
Orzechowski Xavier, Melissa
author_role author
author2 Furtado Mendes, Josiara
Von Groll, Andrea
Baracy Klafke, Gabriel
Martinez Brandolt, Tchana
Orzechowski Xavier, Melissa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues Poester, Vanice
Furtado Mendes, Josiara
Von Groll, Andrea
Baracy Klafke, Gabriel
Martinez Brandolt, Tchana
Orzechowski Xavier, Melissa
description The increase in zoonotic sporotrichosis cases has become a public health problem in hyperendemic areas for the feline disease. Domestic cats involvement in this transmission and the continuous contact of these animals with soil suggest that the environment may be an essential source and/or reservoir of Sporothrix spp. Thus, the current study aimed to evaluate the presence of Sporothrix spp. in the soil of places where infected cats in Southern Brazil is present. In total, 101 soil samples were collected from the residence of cats with sporotrichosis, from regions around to these residences, and also from public places. These samples were processed using the direct plating technique being incubated at 25 °C for up to 15 days. Additionally, seventeen samples were also submitted to DNA extraction (NORGEN BIOTEK CORP(r) kit), PCR-specific and Nested-PCR technique. Although the presence of Sporothrix in soil has been undetected, by culture or molecular biology, this study does not reject the role of the environment in the triad of the infectious process (soil-animal-human). Thus, further studies are necessary for expanding the area of coverage, volume, type and method of sampling, and/or detection techniques.Keywords: Environment; Geophilic; S. brasiliensis; S. schenckii
publishDate 2018
dc.date.none.fl_str_mv 2018-10-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/e-52571
10.1590/cab19052571
url https://revistas.ufg.br/vet/article/view/e-52571
identifier_str_mv 10.1590/cab19052571
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/e-52571/26515
https://revistas.ufg.br/vet/article/view/e-52571/26516
dc.rights.driver.fl_str_mv Copyright (c) 2018 Ciência Animal Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Ciência Animal Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-8
Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-8
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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