In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM

Detalhes bibliográficos
Autor(a) principal: Barbosa, Cristiano Pereira
Data de Publicação: 2013
Outros Autores: Toniollo, Gilson Hélio, Guimarães, Ednaldo Carvalho
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/12588
Resumo: This study aimed to compare the quality and quantity of oocytes aspirated from ovaries containing or not corpus luteum (CL), the development of embryos, pregnancy rate and sex ratio among embryos after IVP in Nellore cows. Follicular aspirations were performed in both ovaries of 219 cows in 250 sections, and equally divided into five groups: G1 = pregnant, G2 = not pregnant, with CL and submitted to hormonal therapy with progestin+BE+PGF2µ; G3 = not pregnant, without CL and with the same treatment of G2; G4 = not pregnant, with the presence of CL and no treatment; G5 = not pregnant, without the presence of CL and no treatment. The statistical analysis was performed with analysis of variance (ANOVA) and Tukey Test was applied to compare means at 5% significance level, by SISVAR program. For the comparison of sex ratio within and between groups, the multiple comparison test of proportions was used. The G4 produced higher mean of total aspirated oocytes than G2 and G3. Comparing the three groups with CL, G4 showed higher number of oocytes than G1 and G2 in both ovaries. The G2 presented more viable oocytes than G1 in the CL-containing ovary. The ovaries without CL showed better production in pregnant cows despite of viable oocytes (p<0.05), and similar embryo production (p>0.05) when compared with ovaries presenting CL. Oocytes obtained from ovaries with CL resulted in better rates of embryo production. Pregnancy rates and sex ratio were similar among groups (p>0.05). The P4 concentration was different between groups, but it did not influence any variable. The use of hormonal treatment did not improve the results of any variable. Therefore, choosing Nellore donors for IVP programs depends on knowing each animal individually along the sections of OPU. KEYWORDS: animal reproduction; follicular aspiration; serum progesterone
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spelling In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUMPRODUÇÃO in vitro DE EMBRIÕES DE BOVINOS DA RAÇA NELORE ORIUNDOS DE OVÓCITOS DE OVÁRIOS COM E SEM CORPO LÚTEOAnimal reproductionbovinocorpo lúteoFIVThis study aimed to compare the quality and quantity of oocytes aspirated from ovaries containing or not corpus luteum (CL), the development of embryos, pregnancy rate and sex ratio among embryos after IVP in Nellore cows. Follicular aspirations were performed in both ovaries of 219 cows in 250 sections, and equally divided into five groups: G1 = pregnant, G2 = not pregnant, with CL and submitted to hormonal therapy with progestin+BE+PGF2µ; G3 = not pregnant, without CL and with the same treatment of G2; G4 = not pregnant, with the presence of CL and no treatment; G5 = not pregnant, without the presence of CL and no treatment. The statistical analysis was performed with analysis of variance (ANOVA) and Tukey Test was applied to compare means at 5% significance level, by SISVAR program. For the comparison of sex ratio within and between groups, the multiple comparison test of proportions was used. The G4 produced higher mean of total aspirated oocytes than G2 and G3. Comparing the three groups with CL, G4 showed higher number of oocytes than G1 and G2 in both ovaries. The G2 presented more viable oocytes than G1 in the CL-containing ovary. The ovaries without CL showed better production in pregnant cows despite of viable oocytes (p<0.05), and similar embryo production (p>0.05) when compared with ovaries presenting CL. Oocytes obtained from ovaries with CL resulted in better rates of embryo production. Pregnancy rates and sex ratio were similar among groups (p>0.05). The P4 concentration was different between groups, but it did not influence any variable. The use of hormonal treatment did not improve the results of any variable. Therefore, choosing Nellore donors for IVP programs depends on knowing each animal individually along the sections of OPU. KEYWORDS: animal reproduction; follicular aspiration; serum progesteroneComparou-se a produção de embriões a partir de ovócitos oriundos de ovários com e sem corpo lúteo de vacas da raça Nelore, em relação à qualidade e quantidade de ovócitos obtidos, embriões produzidos, taxa de prenhez e proporção dos sexos. Foram realizadas aspirações foliculares dos dois ovários de 219 vacas em 250 seções, igualmente distribuídas em cinco grupos, sendo G1=fêmeas gestantes; G2=não gestantes, com CL e submetidas a tratamento hormonal com progestágeno+BE+PGF2µ; G3=não gestantes, sem CL e com o mesmo tratamento de G2; G4=não gestantes, com presença de CL e sem tratamento; G5=não gestantes, sem presença de CL e sem tratamento. Os resultados foram avaliados pela análise de variância (ANOVA) e aplicado o Teste de Tukey para comparação entre médias, a 5% de significância, pelo programa SISVAR. Já para comparação de proporção de sexos dentro e entre os grupos, foi utilizado o teste de comparação múltipla de proporções. O G4 produziu maior média de ovócitos totais aspirados que G2 e G3. Comparando-se os três grupos que apresentavam CL (G1, G2, G4), o G4 foi superior a G1 e G2 em ovócitos totais, no ovário com e sem CL. Em ovócitos viáveis, G2 foi superior a G1 no ovário com CL. Os ovócitos obtidos de ovários com CL é que resultaram em melhores índices de produção de embriões. As vacas gestantes produziram melhor nos ovários sem CL, com mais ovócitos viáveis (p<0,05) e iguais em embriões (p>0,05), em relação ao ovário com CL. As taxas de prenhez e proporção dos sexos foram semelhantes entre os grupos (p>0,05). A concentração de progesterona (P4) foi diferente entre os grupos, mas não influenciou as variáveis analisadas. A utilização do tratamento hormonal não melhorou os resultados de nenhuma variável. Portanto, o melhor critério de escolha de doadoras Nelore para programas de Produção in vitro (PIV) de embriões é o conhecimento individual de cada animal ao longo das seções de aspiração folicular.  PALAVRAS-CHAVE: aspiração folicular; progesterona sérica; reprodução animal.Universidade Federal de Goiás2013-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/1258810.5216/cab.v14i1.12588Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 1 (2013); 81-90Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 1 (2013); 81-901809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/12588/13724https://revistas.ufg.br/vet/article/view/12588/13723Barbosa, Cristiano PereiraToniollo, Gilson HélioGuimarães, Ednaldo Carvalhoinfo:eu-repo/semantics/openAccess2013-04-03T11:54:30Zoai:ojs.revistas.ufg.br:article/12588Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:46.055735Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
PRODUÇÃO in vitro DE EMBRIÕES DE BOVINOS DA RAÇA NELORE ORIUNDOS DE OVÓCITOS DE OVÁRIOS COM E SEM CORPO LÚTEO
title In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
spellingShingle In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
Barbosa, Cristiano Pereira
Animal reproduction
bovino
corpo lúteo
FIV
title_short In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
title_full In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
title_fullStr In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
title_full_unstemmed In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
title_sort In vitro PRODUCTION OF NELORE BOVINE EMBRYOS ORIGINATED FROM OOCYTES FROM OVARIES WITH AND WITHOUT CORPUS LUTEUM
author Barbosa, Cristiano Pereira
author_facet Barbosa, Cristiano Pereira
Toniollo, Gilson Hélio
Guimarães, Ednaldo Carvalho
author_role author
author2 Toniollo, Gilson Hélio
Guimarães, Ednaldo Carvalho
author2_role author
author
dc.contributor.author.fl_str_mv Barbosa, Cristiano Pereira
Toniollo, Gilson Hélio
Guimarães, Ednaldo Carvalho
dc.subject.por.fl_str_mv Animal reproduction
bovino
corpo lúteo
FIV
topic Animal reproduction
bovino
corpo lúteo
FIV
description This study aimed to compare the quality and quantity of oocytes aspirated from ovaries containing or not corpus luteum (CL), the development of embryos, pregnancy rate and sex ratio among embryos after IVP in Nellore cows. Follicular aspirations were performed in both ovaries of 219 cows in 250 sections, and equally divided into five groups: G1 = pregnant, G2 = not pregnant, with CL and submitted to hormonal therapy with progestin+BE+PGF2µ; G3 = not pregnant, without CL and with the same treatment of G2; G4 = not pregnant, with the presence of CL and no treatment; G5 = not pregnant, without the presence of CL and no treatment. The statistical analysis was performed with analysis of variance (ANOVA) and Tukey Test was applied to compare means at 5% significance level, by SISVAR program. For the comparison of sex ratio within and between groups, the multiple comparison test of proportions was used. The G4 produced higher mean of total aspirated oocytes than G2 and G3. Comparing the three groups with CL, G4 showed higher number of oocytes than G1 and G2 in both ovaries. The G2 presented more viable oocytes than G1 in the CL-containing ovary. The ovaries without CL showed better production in pregnant cows despite of viable oocytes (p<0.05), and similar embryo production (p>0.05) when compared with ovaries presenting CL. Oocytes obtained from ovaries with CL resulted in better rates of embryo production. Pregnancy rates and sex ratio were similar among groups (p>0.05). The P4 concentration was different between groups, but it did not influence any variable. The use of hormonal treatment did not improve the results of any variable. Therefore, choosing Nellore donors for IVP programs depends on knowing each animal individually along the sections of OPU. KEYWORDS: animal reproduction; follicular aspiration; serum progesterone
publishDate 2013
dc.date.none.fl_str_mv 2013-03-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/12588
10.5216/cab.v14i1.12588
url https://revistas.ufg.br/vet/article/view/12588
identifier_str_mv 10.5216/cab.v14i1.12588
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/12588/13724
https://revistas.ufg.br/vet/article/view/12588/13723
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 1 (2013); 81-90
Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 1 (2013); 81-90
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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