Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/e-45994 |
Resumo: | The aim of the study was to evaluate the effect of recombinant bovine somatotropin (rbST) and thyroxine (T4) on survival and growth of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovarian fragments were collected in local abattoirs and immediately fixed for classical histology and transmission electron microscopy (non-cultured control). The other fragments were then cultured in situ for seven days in minimum essential medium alone (MEM+ - cultured control) or in the presence of 1,000 ng/mL rbST and 20 ng/mL T4, isolated or associated. After seven days, there was a reduction (P<0.05) in the percentage of normal follicles in MEM+ alone or with T4. In oocyte diameter, there was a reduction in MEM+ alone. There was no influence (P>0.01) of the medium used on the follicular diameter of the PAOF cultured for seven days. Ultrastructural analysis showed cell damage. In conclusion, the presence of rbST maintains the rate of morphologically normal follicles during the culture for seven days (observed by optical microscopy), but it does not exert beneficial effects on its ultrastructural integrity and oocyte and follicular growth. |
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Effect of somatotropin and thyroxine on the in vitro development of bovine preantral folliclesThe aim of the study was to evaluate the effect of recombinant bovine somatotropin (rbST) and thyroxine (T4) on survival and growth of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovarian fragments were collected in local abattoirs and immediately fixed for classical histology and transmission electron microscopy (non-cultured control). The other fragments were then cultured in situ for seven days in minimum essential medium alone (MEM+ - cultured control) or in the presence of 1,000 ng/mL rbST and 20 ng/mL T4, isolated or associated. After seven days, there was a reduction (P<0.05) in the percentage of normal follicles in MEM+ alone or with T4. In oocyte diameter, there was a reduction in MEM+ alone. There was no influence (P>0.01) of the medium used on the follicular diameter of the PAOF cultured for seven days. Ultrastructural analysis showed cell damage. In conclusion, the presence of rbST maintains the rate of morphologically normal follicles during the culture for seven days (observed by optical microscopy), but it does not exert beneficial effects on its ultrastructural integrity and oocyte and follicular growth. Universidade Federal de Goiás2018-04-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-4599410.1590/cab19145994Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-9Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-91809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/vet/article/view/e-45994/25400https://revistas.ufg.br/vet/article/view/e-45994/25401Copyright (c) 2018 Ciência Animal Brasileirainfo:eu-repo/semantics/openAccessFernandes da Silva, TalitaPaulino da Costa, EduardoLourenço da Costa, SanelyDomingos Guimarães, José2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/45994Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:17.741867Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
title |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
spellingShingle |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles Fernandes da Silva, Talita |
title_short |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
title_full |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
title_fullStr |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
title_full_unstemmed |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
title_sort |
Effect of somatotropin and thyroxine on the in vitro development of bovine preantral follicles |
author |
Fernandes da Silva, Talita |
author_facet |
Fernandes da Silva, Talita Paulino da Costa, Eduardo Lourenço da Costa, Sanely Domingos Guimarães, José |
author_role |
author |
author2 |
Paulino da Costa, Eduardo Lourenço da Costa, Sanely Domingos Guimarães, José |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Fernandes da Silva, Talita Paulino da Costa, Eduardo Lourenço da Costa, Sanely Domingos Guimarães, José |
description |
The aim of the study was to evaluate the effect of recombinant bovine somatotropin (rbST) and thyroxine (T4) on survival and growth of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovarian fragments were collected in local abattoirs and immediately fixed for classical histology and transmission electron microscopy (non-cultured control). The other fragments were then cultured in situ for seven days in minimum essential medium alone (MEM+ - cultured control) or in the presence of 1,000 ng/mL rbST and 20 ng/mL T4, isolated or associated. After seven days, there was a reduction (P<0.05) in the percentage of normal follicles in MEM+ alone or with T4. In oocyte diameter, there was a reduction in MEM+ alone. There was no influence (P>0.01) of the medium used on the follicular diameter of the PAOF cultured for seven days. Ultrastructural analysis showed cell damage. In conclusion, the presence of rbST maintains the rate of morphologically normal follicles during the culture for seven days (observed by optical microscopy), but it does not exert beneficial effects on its ultrastructural integrity and oocyte and follicular growth. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-45994 10.1590/cab19145994 |
url |
https://revistas.ufg.br/vet/article/view/e-45994 |
identifier_str_mv |
10.1590/cab19145994 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-45994/25400 https://revistas.ufg.br/vet/article/view/e-45994/25401 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Ciência Animal Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Ciência Animal Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-9 Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-9 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874789380194304 |