IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)

Detalhes bibliográficos
Autor(a) principal: Pereira, José Gomes
Data de Publicação: 2015
Outros Autores: Silva, Silvana Regina, Gonçalves, Mysa Tatiana Corrêa, Melo, Ferdinan Almeida, Viana, Diego Carvalho, Oliveira, Antonia Santos, Machado, Célio Raimundo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/30969
Resumo: The scorpion mud turtle (Kinosternon scorpioides) is a small chelonian, typical of the floodplains of the Amazon. This species is an important source of food and income and it has being studied because of its vulnerability to indiscriminate hunting, deforestation and burning; for this reason, it has been researched in order to supply data for the preservation of the species. The immunohistochemistry activity of endocrine cells present in the stomach of scorpion mud turtle is not totally known. Therefore, the aim of this work was to identify the presence of enteroglucagon hormone and to classify the endocrine cells in the stomach of the scorpion mud turtle. The fragments were submitted to Hematoxylin-Eosin technique and streptavidin peroxidase for staining and detection of antigen, respectively. Immunoreactivity to enteroglucagon in cells was found on the three portions of the stomach (gastric, fundica and pyloric); however, the immunoreactivity was more evident in the first two regions than in the last one. Endocrine cells of stomach were classified as argyrophil and argentaffin and were found among cells that comprise the gastric glands and were classified as “open type” and “closed type”. There is no difference between the biochemistry and immunohistochemistry of enteroglucagon K. scorpioides and other animal species.Keywords: endocrine cells; immunohistochemistry; Kinosternon; turtle. 
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spelling IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)IMUNOLOCALIZAÇÃO DE ENTEROGLUCAGON EM CÉLULAS ENDÓCRINAS PRESENTES NO ESTÔMAGO DO MUÇUÃ Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)Animal SanitySanidade AnimalThe scorpion mud turtle (Kinosternon scorpioides) is a small chelonian, typical of the floodplains of the Amazon. This species is an important source of food and income and it has being studied because of its vulnerability to indiscriminate hunting, deforestation and burning; for this reason, it has been researched in order to supply data for the preservation of the species. The immunohistochemistry activity of endocrine cells present in the stomach of scorpion mud turtle is not totally known. Therefore, the aim of this work was to identify the presence of enteroglucagon hormone and to classify the endocrine cells in the stomach of the scorpion mud turtle. The fragments were submitted to Hematoxylin-Eosin technique and streptavidin peroxidase for staining and detection of antigen, respectively. Immunoreactivity to enteroglucagon in cells was found on the three portions of the stomach (gastric, fundica and pyloric); however, the immunoreactivity was more evident in the first two regions than in the last one. Endocrine cells of stomach were classified as argyrophil and argentaffin and were found among cells that comprise the gastric glands and were classified as “open type” and “closed type”. There is no difference between the biochemistry and immunohistochemistry of enteroglucagon K. scorpioides and other animal species.Keywords: endocrine cells; immunohistochemistry; Kinosternon; turtle. O muçuã ou jurará (Kinosternon scorpioides) é um quelônio de pequeno porte de água doce, nativo das varzéas da região amazônica. Essa espécie é importante fonte de alimentação e renda e vem sendo estudada por apresentar vulnerabilidade em consequência da caça indiscriminada, queimadas e desmatamento e, por esta razão, tem sido fonte de pesquisa para o fornecimento de dados para a preservação da espécie. Ainda não é totalmente compreendida a atividade imunohistoquímica das células endócrinas presentes no estômago do muçuã. Sendo assim, este trabalho tem por objetivo identificar a presença do hormônio enteroglucagon e classificar as células endócrinas do estômago de muçuã. Os fragmentos de estômago foram submetidos à técnica de Hematoxilina-Eosina e da Estreptovidina peroxidase para coloração e detecção do antígeno, respectivamente. Foram encontradas células imunorreativas ao enteroglucagon em uma das três porções gástricas (cárdica, fúndica e pilórica); contudo, nas duas primeiras regiões, a imunoreatividade foi mais evidente do que na última. As células endócrinas do estômago de muçuã foram classificadas em argirófilas e argentafins e encontradas entre as células que compõem as glândulas gástricas, sendo classificadas como do “tipo aberta” e do “tipo fechada”. Não houve diferença bioquímica e imunohistoquímica entre o enteroglucagon do K. scorpioides com as de outras espécies animais.Palavras-chave: células endócrinas; imunohistoquímica; Kinosternon; tartaruga. Universidade Federal de Goiás2015-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/3096910.1590/cab.v16i3.30969Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 3 (2015); 448-455Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 3 (2015); 448-4551809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/30969/18835https://revistas.ufg.br/vet/article/view/30969/18836Pereira, José GomesSilva, Silvana ReginaGonçalves, Mysa Tatiana CorrêaMelo, Ferdinan AlmeidaViana, Diego CarvalhoOliveira, Antonia SantosMachado, Célio Raimundoinfo:eu-repo/semantics/openAccess2015-07-31T20:01:58Zoai:ojs.revistas.ufg.br:article/30969Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:06.038130Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
IMUNOLOCALIZAÇÃO DE ENTEROGLUCAGON EM CÉLULAS ENDÓCRINAS PRESENTES NO ESTÔMAGO DO MUÇUÃ Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
title IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
spellingShingle IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
Pereira, José Gomes
Animal Sanity
Sanidade Animal
title_short IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
title_full IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
title_fullStr IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
title_full_unstemmed IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
title_sort IMMUNOLOCALIZATION OF ENTEROGLUCAGON IN ENDOCRINE CELLS IN THE STOMACH OF SCORPION MUD TURTLE Kinosternon scorpioides (REPTILIA, CHELONIA, KINOSTERNIDAE)
author Pereira, José Gomes
author_facet Pereira, José Gomes
Silva, Silvana Regina
Gonçalves, Mysa Tatiana Corrêa
Melo, Ferdinan Almeida
Viana, Diego Carvalho
Oliveira, Antonia Santos
Machado, Célio Raimundo
author_role author
author2 Silva, Silvana Regina
Gonçalves, Mysa Tatiana Corrêa
Melo, Ferdinan Almeida
Viana, Diego Carvalho
Oliveira, Antonia Santos
Machado, Célio Raimundo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, José Gomes
Silva, Silvana Regina
Gonçalves, Mysa Tatiana Corrêa
Melo, Ferdinan Almeida
Viana, Diego Carvalho
Oliveira, Antonia Santos
Machado, Célio Raimundo
dc.subject.por.fl_str_mv Animal Sanity
Sanidade Animal
topic Animal Sanity
Sanidade Animal
description The scorpion mud turtle (Kinosternon scorpioides) is a small chelonian, typical of the floodplains of the Amazon. This species is an important source of food and income and it has being studied because of its vulnerability to indiscriminate hunting, deforestation and burning; for this reason, it has been researched in order to supply data for the preservation of the species. The immunohistochemistry activity of endocrine cells present in the stomach of scorpion mud turtle is not totally known. Therefore, the aim of this work was to identify the presence of enteroglucagon hormone and to classify the endocrine cells in the stomach of the scorpion mud turtle. The fragments were submitted to Hematoxylin-Eosin technique and streptavidin peroxidase for staining and detection of antigen, respectively. Immunoreactivity to enteroglucagon in cells was found on the three portions of the stomach (gastric, fundica and pyloric); however, the immunoreactivity was more evident in the first two regions than in the last one. Endocrine cells of stomach were classified as argyrophil and argentaffin and were found among cells that comprise the gastric glands and were classified as “open type” and “closed type”. There is no difference between the biochemistry and immunohistochemistry of enteroglucagon K. scorpioides and other animal species.Keywords: endocrine cells; immunohistochemistry; Kinosternon; turtle. 
publishDate 2015
dc.date.none.fl_str_mv 2015-07-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/30969
10.1590/cab.v16i3.30969
url https://revistas.ufg.br/vet/article/view/30969
identifier_str_mv 10.1590/cab.v16i3.30969
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/30969/18835
https://revistas.ufg.br/vet/article/view/30969/18836
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 3 (2015); 448-455
Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 3 (2015); 448-455
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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